FastQCFastQC Report
Thu 26 May 2016
SRR1043401_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043401_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2312724
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT694803.0042495343153788No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC165330.7148712946291905No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC77740.33614041277731366No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76070.32891949060934206No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC74870.32373080402157806No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC29850.1290685788706305No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29100.12582564975327795No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA28020.12115583182429031No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAA207.0347695E-445.029
ATACGTT253.8920356E-545.013
CGACGGT10350.044.5652227
CGGTCTA10400.044.3509630
CACGACG10500.044.1428625
CGTTTTT210800.043.8152281
TCACGAC10900.043.7614724
CTTGCGG6350.042.5196841
ACGTAGG2650.042.452831
CTAACCT11200.042.38839714
GACGGTC11100.041.55405428
TTCCGTA1900.041.4473745
ACGGGCT8100.041.3888854
ACGGTCT11200.041.3839329
CACTTAC54350.041.14995636
CTTACTG54000.041.08333238
CGCACTT54350.041.0257634
CCGCACT54400.040.94669333
CTAGCGG8600.040.8139531
GCGCGAC25400.040.748038