##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043401_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2312724
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.512295457650804	31.0	31.0	33.0	30.0	34.0
2	30.56333224370915	31.0	31.0	34.0	26.0	34.0
3	31.54513595223641	31.0	31.0	34.0	28.0	34.0
4	35.159217009898285	37.0	35.0	37.0	32.0	37.0
5	35.48456149544866	37.0	35.0	37.0	33.0	37.0
6	35.80047986703126	37.0	35.0	37.0	35.0	37.0
7	33.77473446896387	37.0	35.0	37.0	32.0	37.0
8	34.75271238591375	37.0	35.0	37.0	31.0	37.0
9	36.51954405281391	39.0	35.0	39.0	32.0	39.0
10	36.59601491574438	39.0	35.0	39.0	32.0	39.0
11	36.33161371612004	38.0	35.0	39.0	32.0	39.0
12	36.29766716651014	39.0	35.0	39.0	32.0	39.0
13	36.33899591996278	39.0	35.0	39.0	32.0	39.0
14	37.53352842794904	40.0	35.0	41.0	33.0	41.0
15	37.61283836722411	40.0	35.0	41.0	33.0	41.0
16	37.425575641537854	40.0	35.0	41.0	32.0	41.0
17	37.37257580238714	39.0	35.0	41.0	32.0	41.0
18	37.19282888922327	39.0	35.0	41.0	32.0	41.0
19	37.07635887377828	38.0	35.0	41.0	32.0	41.0
20	36.8870556970914	38.0	35.0	41.0	32.0	41.0
21	36.81278137814975	38.0	35.0	41.0	32.0	41.0
22	36.74720632466304	38.0	35.0	40.0	32.0	41.0
23	36.71957224467771	38.0	35.0	40.0	32.0	41.0
24	36.61232425486137	38.0	35.0	40.0	32.0	41.0
25	36.53593770808795	38.0	35.0	40.0	32.0	41.0
26	36.284946236559136	38.0	35.0	40.0	31.0	41.0
27	36.164195987069796	37.0	35.0	40.0	31.0	41.0
28	36.02210639920717	37.0	35.0	40.0	31.0	41.0
29	35.90883607382463	37.0	35.0	40.0	30.0	41.0
30	35.73040189836747	37.0	34.0	40.0	30.0	41.0
31	35.51582462931158	37.0	34.0	40.0	29.0	41.0
32	35.3874573879114	37.0	34.0	40.0	28.0	41.0
33	35.17525221340722	37.0	34.0	40.0	26.0	41.0
34	35.05712008869195	37.0	34.0	40.0	25.0	41.0
35	34.94929139836833	37.0	34.0	40.0	25.0	41.0
36	34.906113310537705	37.0	34.0	40.0	25.0	41.0
37	34.81215743858757	37.0	34.0	40.0	24.0	41.0
38	34.745808838408735	37.0	34.0	40.0	24.0	41.0
39	34.64331325311624	37.0	34.0	40.0	23.0	41.0
40	34.590342816522856	36.0	34.0	40.0	23.0	41.0
41	34.537767152500685	36.0	34.0	40.0	23.0	41.0
42	34.45390024922991	36.0	34.0	40.0	23.0	41.0
43	34.30182935793463	36.0	34.0	40.0	23.0	41.0
44	34.09558468714814	35.0	33.0	40.0	23.0	41.0
45	33.88358792488857	35.0	33.0	40.0	22.0	41.0
46	33.87888048898182	35.0	33.0	40.0	22.0	41.0
47	33.85896155356195	35.0	33.0	40.0	22.0	41.0
48	33.77728427603121	35.0	33.0	40.0	22.0	41.0
49	33.73835269578212	35.0	33.0	40.0	22.0	41.0
50	33.67515708748645	35.0	33.0	40.0	22.0	41.0
51	33.280347330680186	35.0	33.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	6.0
12	10.0
13	20.0
14	56.0
15	99.0
16	254.0
17	532.0
18	1089.0
19	2093.0
20	3596.0
21	5770.0
22	9144.0
23	13740.0
24	21321.0
25	31040.0
26	41483.0
27	48040.0
28	53552.0
29	57956.0
30	66096.0
31	76322.0
32	91988.0
33	120326.0
34	226625.0
35	250661.0
36	206506.0
37	229363.0
38	305427.0
39	449374.0
40	230.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.394558105506754	17.96526520241931	24.879190080614894	27.760986611459042
2	30.777299842090972	21.569499862499804	28.511832799763397	19.141367495645827
3	26.936115161169255	21.541351237761187	32.770101404231546	18.752432196838015
4	23.44486415153732	24.90020426129534	30.17748767254545	21.47744391462189
5	21.539578436510364	34.004014313856736	28.2852169130428	16.1711903365901
6	82.94288466760409	2.1144330235687443	13.591332126098921	1.351350182728246
7	78.87845674624381	7.225635224955507	12.427682680683038	1.468225348117631
8	77.51703186372434	3.429419161127744	16.138934001636166	2.914614973511755
9	41.572881156592835	17.709030563093563	28.432099982531422	12.285988297782183
10	34.86568219986475	19.54042073329978	27.80725240019994	17.786644666635535
11	31.530221505030433	17.486695342807877	34.3279180740979	16.655165078063792
12	24.48943323976402	20.277862814585742	34.61956549938514	20.613138446265097
13	18.605116736800415	24.23293916610888	38.10212545898256	19.059818638108137
14	20.798547513667863	20.723398036255084	40.0387594888106	18.439294961266455
15	27.277617216753924	17.004666358804595	37.99887059588606	17.71884582855542
16	24.53868252329288	19.874139759002805	34.34322469953181	21.24395301817251
17	25.59008338219347	21.366449260698637	34.982297930924744	18.06116942618315
18	24.24777016193891	26.63910609307466	31.96542259257914	17.14770115240729
19	26.627388309197293	23.552961788782405	31.06600701164514	18.75364289037516
20	25.257920962466773	26.905285715026956	32.71077742091145	15.12601590159483
21	21.88752311127484	21.66151257132282	35.85071110949685	20.60025320790548
22	22.684375653990703	22.489713428839757	34.71949095525449	20.10641996191504
23	25.331643551067916	21.05482539204851	36.38748073700104	17.22605031988253
24	25.019544052813913	19.20946900711023	33.760708151945494	22.01027878813036
25	18.892224061323358	22.190801842329652	34.86741176206067	24.04956233428632
26	17.347768259420494	21.26682647821357	36.366120643881416	25.019284618484523
27	18.13532440533328	24.835734830442373	36.10750785653627	20.921432907688075
28	22.638715212018383	24.909976287702293	33.06659160366736	19.384716896611963
29	22.362633846494436	22.24536952961097	36.384324285993486	19.00767233790111
30	27.979862707352886	21.595745968822914	32.26627993655966	18.15811138726454
31	28.165098818536066	22.092605948656217	32.27609520202151	17.466200030786208
32	26.361511360629287	22.209913504594585	32.147804926139045	19.280770208637087
33	20.94711690629751	23.647957992393383	35.27070242709463	20.134222674214474
34	20.346396716599127	26.48828826959032	32.93004266829938	20.23527234551118
35	22.82870761924034	30.505369425837237	28.98642466632421	17.679498288598207
36	23.31890878461935	28.337017300810647	28.428511140974884	19.91556277359512
37	22.659124045930255	31.282504959519596	27.324185678879104	18.734185315671045
38	22.742315987554072	27.613498195201846	27.420954683740906	22.223231133503177
39	21.6773380654155	22.706643767263195	31.668456763539442	23.94756140378186
40	18.563996395592383	25.02901340583658	32.17011627846643	24.236873920104603
41	18.627168654798414	27.426489282767854	28.47789014166844	25.468451920765293
42	18.13091402173368	22.790181621326195	31.58353525971971	27.49536909722042
43	20.14377850534694	21.85440199522295	33.64353031317183	24.358289186258283
44	21.6421847137834	25.484234175803078	30.85236284139396	22.021218269019563
45	22.553101883320277	25.391918793595774	30.85936756828744	21.19561175479651
46	20.530984241958834	25.11475645169938	30.773754239589334	23.58050506675245
47	18.67248318433155	25.841042856821655	33.93889629718029	21.5475776616665
48	21.838533262075373	22.927076469133368	33.75664368078508	21.477746588006177
49	19.724229955671323	22.691856010488067	34.81755713176324	22.766356902077376
50	17.661943232309604	23.354018897196553	35.76306554521854	23.2209723252753
51	16.57906434144325	21.323426401075096	35.520235012911186	26.57727424457047
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1662.0
1	10610.0
2	19558.0
3	67406.5
4	115255.0
5	69903.5
6	24552.0
7	21801.0
8	19050.0
9	19694.5
10	20339.0
11	20723.0
12	21107.0
13	20171.5
14	19236.0
15	17552.0
16	15868.0
17	14642.5
18	13417.0
19	12672.0
20	11927.0
21	11646.0
22	11365.0
23	10530.0
24	9695.0
25	10933.0
26	13947.0
27	15723.0
28	18740.5
29	21758.0
30	24787.0
31	27816.0
32	30994.0
33	34172.0
34	39516.5
35	44861.0
36	47651.5
37	50442.0
38	56504.0
39	62566.0
40	70404.0
41	78242.0
42	86894.0
43	95546.0
44	103420.0
45	111294.0
46	121580.0
47	131866.0
48	149094.5
49	166323.0
50	161400.0
51	156477.0
52	145448.5
53	134420.0
54	136488.0
55	138556.0
56	136521.0
57	134486.0
58	132362.0
59	130238.0
60	124680.0
61	119122.0
62	114261.0
63	109400.0
64	96484.5
65	83569.0
66	68028.5
67	52488.0
68	41452.0
69	30416.0
70	27095.0
71	23774.0
72	21709.5
73	19645.0
74	16177.0
75	9726.5
76	6744.0
77	4573.0
78	2402.0
79	1806.0
80	1210.0
81	958.0
82	706.0
83	531.0
84	356.0
85	221.5
86	87.0
87	61.0
88	35.0
89	36.0
90	37.0
91	30.0
92	23.0
93	13.0
94	3.0
95	6.0
96	9.0
97	4.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2312724.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.048782666993382
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.3149243573409	20.13959661499406
2	9.704459579880464	5.055787169938562
3	3.2675126674649126	2.55344182009324
4	1.591277628828635	1.658033804648227
5	0.9676967518711188	1.26036611885231
6	0.6611957000646865	1.0334005854801338
7	0.4730653440584863	0.8625943434266172
8	0.353105972921833	0.7358384597646462
9	0.30373218859611467	0.7120668392739393
>10	3.8244531693578434	25.114764745528483
>50	1.2196537661425466	21.84480941105785
>100	0.308623820388149	11.76220025729055
>500	0.00680784864909041	1.1593238429522885
>1k	0.0026184033265732347	1.1568684190615048
>5k	5.23680665314647E-4	1.039826546934144
>10k+	3.4912044354309794E-4	3.911081020703451
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	69480	3.0042495343153788	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	16533	0.7148712946291905	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	7774	0.33614041277731366	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7607	0.32891949060934206	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	7487	0.32373080402157806	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2985	0.1290685788706305	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2910	0.12582564975327795	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	2802	0.12115583182429031	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05050321612090332	0.0
2	0.0	0.0	0.0	0.16707570812600206	0.0
3	0.0	0.0	0.0	0.258180396796159	0.0
4	0.0	0.0	0.0	0.3204446358493275	0.0
5	0.0	0.0	0.0	0.6883225149217979	0.0
6	0.0	0.0	0.0	0.784875324509107	0.0
7	0.0	0.0	0.0	1.082705934646763	0.0
8	0.0	0.0	0.0	1.4197976066318332	0.0
9	0.0	0.0	0.0	1.709758708778047	0.0
10	0.0	0.0	0.0	1.9174791285082007	0.0
11	0.0	0.0	0.0	2.156461384929633	0.0
12	0.0	0.0	0.0	2.3496967212689452	0.0
13	0.0	0.0	0.0	2.4493195037540145	0.0
14	0.0	0.0	0.0	2.52572291375884	0.0
15	0.0	0.0	0.0	2.7772877351556002	0.0
16	0.0	0.0	0.0	3.071010635077943	0.0
17	0.0	0.0	0.0	3.410480455082405	0.0
18	0.0	0.0	0.0	3.6150444238050023	0.0
19	0.0	0.0	0.0	3.851562054097246	0.0
20	0.0	0.0	0.0	4.117179568335867	0.0
21	0.0	0.0	0.0	4.370128039489364	0.0
22	0.0	0.0	0.0	4.619703864360814	0.0
23	0.0	0.0	0.0	4.860545400142862	0.0
24	8.647810979606732E-5	0.0	0.0	5.067703712159341	0.0
25	8.647810979606732E-5	0.0	0.0	5.273608091583777	0.0
26	8.647810979606732E-5	0.0	0.0	5.488981824030883	0.0
27	8.647810979606732E-5	0.0	0.0	5.723596935907613	0.0
28	8.647810979606732E-5	0.0	0.0	5.961109064462512	0.0
29	8.647810979606732E-5	0.0	0.0	6.2076581554911	0.0
30	8.647810979606732E-5	0.0	0.0	6.456542155484183	0.0
31	8.647810979606732E-5	0.0	0.0	6.6973836912662295	0.0
32	8.647810979606732E-5	0.0	0.0	6.992447001890412	0.0
33	8.647810979606732E-5	0.0	0.0	7.267966259700682	0.0
34	8.647810979606732E-5	0.0	0.0	7.578898303472442	0.0
35	8.647810979606732E-5	0.0	0.0	7.849315352804744	0.0
36	8.647810979606732E-5	0.0	0.0	8.13218524994768	0.0
37	8.647810979606732E-5	0.0	0.0	8.52116378781039	0.0
38	8.647810979606732E-5	0.0	0.0	8.908931632135957	0.0
39	8.647810979606732E-5	0.0	0.0	9.374702731497576	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATAA	20	7.0347695E-4	45.0	29
ATACGTT	25	3.8920356E-5	45.0	13
CGACGGT	1035	0.0	44.56522	27
CGGTCTA	1040	0.0	44.35096	30
CACGACG	1050	0.0	44.14286	25
CGTTTTT	21080	0.0	43.815228	1
TCACGAC	1090	0.0	43.76147	24
CTTGCGG	635	0.0	42.519684	1
ACGTAGG	265	0.0	42.45283	1
CTAACCT	1120	0.0	42.388397	14
GACGGTC	1110	0.0	41.554054	28
TTCCGTA	190	0.0	41.44737	45
ACGGGCT	810	0.0	41.388885	4
ACGGTCT	1120	0.0	41.38393	29
CACTTAC	5435	0.0	41.149956	36
CTTACTG	5400	0.0	41.083332	38
CGCACTT	5435	0.0	41.02576	34
CCGCACT	5440	0.0	40.946693	33
CTAGCGG	860	0.0	40.813953	1
GCGCGAC	2540	0.0	40.74803	8
>>END_MODULE