FastQCFastQC Report
Thu 26 May 2016
SRR1043400_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043400_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1685961
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT657733.901217169317677No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC115360.6842388406374762No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74680.44295212048202776No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC56370.3343493710708611No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC47270.2803742198069825No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA37730.2237892810094658No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30780.1825665006485915No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC27210.16139163361430067No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC24990.1482240692400358No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG23820.14128440693468No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTATG302.1661072E-645.00000444
CGACGTA207.034226E-445.036
TATGCGA207.034226E-445.01
GTATATC207.034226E-445.07
AATACGG253.8915834E-545.01
ATACCCG253.8915834E-545.038
TCTATCG207.034226E-445.025
CAGACGA351.2123746E-745.01
ACATACG3850.044.41558516
ACGGGTA2250.044.04
CGTTTTT158400.043.9914781
CATGCGG3200.043.593751
TCACGAC12350.042.81376324
TTAACGG900.042.5000041
ATCGGGT850.042.352943
CATATGC11550.042.27272832
CACTTAC40050.042.24719236
TAGTAGG4750.042.1578941
ACGGTCT12100.042.0247929
CGCACTT40150.041.9738534