##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043399_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3158269
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58421844371078	31.0	31.0	33.0	30.0	34.0
2	30.60537876919287	31.0	31.0	34.0	26.0	34.0
3	31.606012344103686	31.0	31.0	34.0	28.0	34.0
4	35.22591235895359	37.0	35.0	37.0	33.0	37.0
5	35.553875873144435	37.0	35.0	37.0	33.0	37.0
6	35.78490179272253	37.0	35.0	37.0	35.0	37.0
7	33.659210155943015	37.0	35.0	37.0	32.0	37.0
8	34.72383321370029	37.0	35.0	37.0	31.0	37.0
9	36.792808655627496	39.0	37.0	39.0	32.0	39.0
10	36.81089451215207	39.0	35.0	39.0	32.0	39.0
11	36.682765464246394	39.0	35.0	39.0	33.0	39.0
12	36.69355586873696	39.0	35.0	39.0	33.0	39.0
13	36.71586967417912	39.0	35.0	39.0	33.0	39.0
14	37.97651624988245	40.0	36.0	41.0	33.0	41.0
15	38.05463467488045	40.0	36.0	41.0	33.0	41.0
16	37.89389187558121	40.0	36.0	41.0	33.0	41.0
17	37.85378414568233	40.0	36.0	41.0	33.0	41.0
18	37.67014969275891	39.0	36.0	41.0	33.0	41.0
19	37.46172824417426	39.0	36.0	41.0	33.0	41.0
20	37.21015625964729	39.0	35.0	41.0	33.0	41.0
21	37.1113078081696	39.0	35.0	41.0	32.0	41.0
22	37.0329234146933	38.0	35.0	41.0	32.0	41.0
23	36.96777950199935	38.0	35.0	41.0	32.0	41.0
24	36.82820146099018	38.0	35.0	40.0	32.0	41.0
25	36.721094055002915	38.0	35.0	40.0	32.0	41.0
26	36.45484377676506	38.0	35.0	40.0	31.0	41.0
27	36.32579302143041	38.0	35.0	40.0	31.0	41.0
28	36.15787192287927	38.0	35.0	40.0	31.0	41.0
29	36.05178184632151	38.0	35.0	40.0	30.0	41.0
30	35.88060136739461	38.0	35.0	40.0	30.0	41.0
31	35.67349171334044	38.0	35.0	40.0	29.0	41.0
32	35.54661620020334	38.0	35.0	40.0	28.0	41.0
33	35.266424107636176	38.0	34.0	40.0	26.0	41.0
34	35.134277035933295	38.0	34.0	40.0	25.0	41.0
35	34.98632542066556	37.0	34.0	40.0	25.0	41.0
36	34.86150293087764	37.0	34.0	40.0	24.0	41.0
37	34.719832921134966	37.0	34.0	40.0	24.0	41.0
38	34.58230853673326	37.0	34.0	40.0	23.0	41.0
39	34.447558456863554	37.0	34.0	40.0	23.0	41.0
40	34.33851233064695	36.0	34.0	40.0	23.0	41.0
41	34.22886144277134	36.0	34.0	40.0	23.0	41.0
42	34.10283829528137	36.0	33.0	40.0	23.0	41.0
43	33.919520788127926	35.0	33.0	40.0	22.0	41.0
44	33.70685239287724	35.0	33.0	40.0	21.0	41.0
45	33.47836267271724	35.0	33.0	40.0	20.0	41.0
46	33.47825185251794	35.0	33.0	40.0	20.0	41.0
47	33.43389369303248	35.0	33.0	39.0	21.0	41.0
48	33.34322408889173	35.0	33.0	39.0	20.0	41.0
49	33.289077972775594	35.0	33.0	39.0	20.0	41.0
50	33.219368584499925	35.0	33.0	39.0	20.0	41.0
51	32.76662849174659	35.0	32.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	10.0
12	19.0
13	33.0
14	48.0
15	148.0
16	279.0
17	695.0
18	1439.0
19	2714.0
20	4918.0
21	7786.0
22	12383.0
23	18928.0
24	29432.0
25	43881.0
26	58771.0
27	69168.0
28	74512.0
29	77277.0
30	83773.0
31	96727.0
32	117856.0
33	156968.0
34	278207.0
35	313055.0
36	328031.0
37	367703.0
38	453275.0
39	559918.0
40	312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.355890837670888	17.107947423097905	24.124575835687207	29.411585903543997
2	31.921821732094386	20.419888236245868	26.824187553371797	20.834102478287946
3	25.920496322510843	21.73009962102658	33.050762933746306	19.298641122716273
4	23.441131835192	24.62494486695085	30.23836791609581	21.695555381761338
5	21.906303737901997	33.19967361868162	28.07142140204017	16.82260124137621
6	81.79220959329302	2.1995276526477	14.563040703625942	1.4452220504333229
7	77.26491948595893	7.576935340213263	13.624741907671575	1.5334032661562393
8	76.79048238132977	3.65282374617235	16.489159093161476	3.0675347793364023
9	44.236827198696496	18.110173642587128	22.936108355558062	14.71689080315831
10	28.11194993206722	19.049422325964002	30.25866384402342	22.579963897945362
11	25.03311149240296	15.620328730706598	39.55125418385831	19.795305593032133
12	21.312275806778967	17.375245743791933	39.5677188991818	21.744759550247302
13	18.02721680768801	20.657296766044944	41.55969615001129	19.75579027625576
14	18.266968393129275	18.83512772344598	41.55323691553823	21.34466696788652
15	22.989333714132645	15.418604305079775	40.2610417288711	21.331020251916478
16	22.95231976756888	16.500779382630167	35.96321275990107	24.58368808989988
17	22.93367031117362	19.674004969177737	35.30728383174454	22.085040887904103
18	24.3635041853623	22.67102643884989	31.969949361501506	20.995520014286306
19	25.463188854400936	20.733351085673828	31.63923022389796	22.164229836027268
20	26.01181216672804	21.824106812940887	33.72831763222195	18.43576338810912
21	23.051994621104154	18.52878902968683	34.33133783094473	24.087878518264276
22	24.33032145140265	20.345702028547915	33.80253550283399	21.52144101721544
23	24.978524628522777	19.986327953698687	34.48338314437434	20.551764273404196
24	26.15173058406361	17.77429344998795	32.914264111131764	23.159711854816674
25	21.250533124315883	19.900489793617957	33.58032517179505	25.2686519102711
26	20.07960689858907	20.11858394582602	35.244907890999784	24.556901264585125
27	21.480944150102477	22.037736494263154	34.898642262581184	21.58267709305319
28	23.326195457068412	22.540860199052076	32.42684521172832	21.706099132151188
29	22.428836809024183	19.79989671557426	36.150087278822674	21.621179196578886
30	26.95451210774003	20.636050950694827	31.746915794696402	20.66252114686874
31	27.784428748786123	20.999857833515765	30.4716919299781	20.744021487720012
32	27.15452673600634	19.863760813280944	29.757503239907685	23.224209210805032
33	22.610360295465647	22.787260996450904	32.60612063126985	21.996258076813596
34	23.275376479964184	23.41614979598001	31.350717750767902	21.9577559732879
35	25.93170499409645	25.939209104734275	27.857506754491148	20.271579146678135
36	25.015095294289374	25.287839636205785	27.843543409380267	21.85352166012458
37	24.942682209780102	27.189450930240582	27.028603326695734	20.83926353328358
38	24.103045054110336	23.93551657569384	27.289379087088527	24.6720592831073
39	23.508700493846472	22.50707586972484	31.648095839841382	22.336127796587306
40	21.194901384270942	23.410988740984383	31.60658575947774	23.78752411526694
41	21.438230879003655	26.182095318669816	28.317410581555908	24.062263220770618
42	21.20569843797346	21.882936507308276	29.667612226824254	27.243752827894014
43	22.212674094575224	21.674341229325307	32.243168647129174	23.869816028970302
44	24.39301402128824	23.502177933545244	29.91961736001588	22.185190685150634
45	24.523750193539563	23.559044527239447	30.20638837287134	21.71081690634965
46	22.572618101877957	23.791418653699225	30.33791611797475	23.29804712644806
47	21.63374937347009	25.870912199055873	31.23815609120059	21.257182336273445
48	23.042749050191734	25.171003483237183	30.45367573186451	21.332571734706576
49	21.25898712237621	23.018526920917758	32.49720020682215	23.225285749883877
50	20.940679847093456	23.714794401616835	32.944723834480214	22.39980191680949
51	20.820297447747485	21.882588215253357	32.48184369349159	24.815270643507567
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1449.0
1	13395.0
2	25341.0
3	92124.5
4	158908.0
5	97539.0
6	36170.0
7	33110.5
8	30051.0
9	29970.0
10	29889.0
11	29984.0
12	30079.0
13	28771.5
14	27464.0
15	25402.0
16	23340.0
17	21438.5
18	19537.0
19	18198.0
20	16859.0
21	16716.5
22	16574.0
23	16007.5
24	15441.0
25	15960.5
26	17987.5
27	19495.0
28	21666.5
29	23838.0
30	25815.5
31	27793.0
32	31946.0
33	36099.0
34	42662.0
35	49225.0
36	54424.0
37	59623.0
38	61800.5
39	63978.0
40	72046.5
41	80115.0
42	92610.0
43	105105.0
44	114904.0
45	124703.0
46	136000.5
47	147298.0
48	163932.0
49	180566.0
50	175323.5
51	170081.0
52	157506.5
53	144932.0
54	152865.0
55	160798.0
56	170515.5
57	180233.0
58	189323.0
59	198413.0
60	200007.0
61	201601.0
62	197387.0
63	193173.0
64	178369.0
65	163565.0
66	139576.0
67	115587.0
68	96505.5
69	77424.0
70	69160.0
71	60896.0
72	55951.5
73	51007.0
74	43314.0
75	27779.0
76	19937.0
77	15050.5
78	10164.0
79	7671.0
80	5178.0
81	3799.5
82	2421.0
83	1686.0
84	951.0
85	687.0
86	423.0
87	326.5
88	230.0
89	184.5
90	139.0
91	96.5
92	54.0
93	34.0
94	14.0
95	9.5
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3158269.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.834223187696914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.8500359274202	19.085109442040793
2	8.88504386886799	4.413063249438915
3	2.912185201669709	2.169655717865211
4	1.4930541677755977	1.4831536173544106
5	0.9658146072664998	1.199262775739705
6	0.651484005805601	0.9707459522034676
7	0.5254411300310209	0.9134245606630234
8	0.44826373429516003	0.8905825299549178
9	0.3723984740960197	0.8323404138412489
>10	5.477207812318871	33.24978826828327
>50	1.2340326083839852	20.474306877334254
>100	0.17559077530210568	6.498755318980661
>500	0.006161534848281729	0.9764520461898527
>1k	0.002601536935941175	1.3965345290784665
>5k	1.3692299662848288E-4	0.2774366988739033
>10k+	5.476919865139315E-4	5.169388002157897
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	104008	3.2931963680104515	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	26588	0.8418535596556216	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11340	0.3590574457083928	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	10088	0.31941547727568487	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8159	0.25833771600835775	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4769	0.1510004372648435	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4605	0.14580771935512776	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	4160	0.13171772258791128	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3973	0.125796757654272	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	3166	0.10024478598878056	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.035335812117333894	0.0
2	0.0	0.0	0.0	0.11756439999252755	0.0
3	0.0	0.0	0.0	0.19339707922282745	0.0
4	0.0	0.0	0.0	0.24168302320036703	0.0
5	0.0	0.0	0.0	0.5214881949574276	0.0
6	0.0	0.0	0.0	0.6377860783866098	0.0
7	0.0	0.0	0.0	0.9323145051925597	0.0
8	0.0	0.0	0.0	1.2431176698374964	0.0
9	0.0	0.0	0.0	1.5471133079544523	0.0
10	0.0	0.0	0.0	1.7341778043605531	0.0
11	0.0	0.0	0.0	1.9233003901820902	0.0
12	0.0	0.0	0.0	2.07357258042301	0.0
13	0.0	0.0	0.0	2.1540280451095204	0.0
14	0.0	0.0	0.0	2.2120345037107354	0.0
15	0.0	0.0	0.0	2.3700957708162287	0.0
16	0.0	0.0	0.0	2.5473764267704873	0.0
17	0.0	0.0	0.0	2.790959224815872	0.0
18	3.166291408363252E-5	0.0	0.0	2.9248616884755543	0.0
19	3.166291408363252E-5	0.0	0.0	3.081529787361368	0.0
20	3.166291408363252E-5	0.0	0.0	3.2488049624651985	0.0
21	3.166291408363252E-5	0.0	0.0	3.428080382006726	0.0
22	3.166291408363252E-5	0.0	0.0	3.6028280048342936	0.0
23	3.166291408363252E-5	0.0	0.0	3.764467181231238	0.0
24	9.498874225089756E-5	0.0	0.0	3.9034990369724683	0.0
25	9.498874225089756E-5	0.0	0.0	4.043480780136208	0.0
26	9.498874225089756E-5	0.0	0.0	4.196855935957323	0.0
27	9.498874225089756E-5	0.0	0.0	4.348394642761589	0.0
28	9.498874225089756E-5	0.0	0.0	4.510730403268373	0.0
29	9.498874225089756E-5	0.0	0.0	4.680981892296065	0.0
30	9.498874225089756E-5	0.0	0.0	4.852626549543436	0.0
31	9.498874225089756E-5	0.0	0.0	5.023194667711965	0.0
32	9.498874225089756E-5	0.0	0.0	5.220802914507916	0.0
33	9.498874225089756E-5	0.0	0.0	5.4169863301701024	0.0
34	9.498874225089756E-5	0.0	0.0	5.638246773786527	0.0
35	9.498874225089756E-5	0.0	0.0	5.836203312637397	0.0
36	9.498874225089756E-5	0.0	0.0	6.052366027086356	0.0
37	1.583145704181626E-4	0.0	0.0	6.318999426584626	0.0
38	1.583145704181626E-4	0.0	0.0	6.6171690885101935	0.0
39	1.583145704181626E-4	0.0	0.0	6.941523980382925	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGA	30	2.166711E-6	45.000004	1
GACGTAT	20	7.035162E-4	45.0	37
CGTTTTT	31185	0.0	43.59307	1
ACTACGG	190	0.0	42.63158	1
TACGGGT	335	0.0	42.313435	3
CACTTAC	8240	0.0	41.204494	36
CTTACTG	8245	0.0	40.96119	38
TAGCATA	2105	0.0	40.831352	29
ACTTACT	8330	0.0	40.65126	37
CGCACTT	8350	0.0	40.607784	34
AAACACG	710	0.0	40.56338	39
GGTACCT	2065	0.0	40.423725	7
ATCCTGC	2200	0.0	40.295456	19
CGTTTTA	1620	0.0	40.27778	1
CATATGC	2140	0.0	40.268692	32
CATGCGG	605	0.0	40.16529	1
GCACTTA	8465	0.0	40.162437	35
CCGCACT	8465	0.0	40.162437	33
GCATATG	2165	0.0	40.115475	31
ACCCGCA	8590	0.0	39.630383	31
>>END_MODULE