FastQCFastQC Report
Thu 26 May 2016
SRR1043398_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043398_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences779695
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT453765.819711553876837No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48600.6233206574365617No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19120.24522409403677078No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT11200.14364591282488665No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8790.11273639051167443No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG1350.045.0000041
ACTATTG207.031889E-445.016
AGCCGGA253.889647E-545.010
GTACCGG453.8380676E-1045.01
GATAATG207.031889E-445.08
CCCTCGA207.031889E-445.020
ATTAGCG207.031889E-445.09
CTCCGGT406.8102963E-945.030
CTCCGAT207.031889E-445.014
CACGGCG207.031889E-445.03
GCGAATA253.889647E-545.027
TTAGCGT207.031889E-445.010
GACAATC207.031889E-445.030
CGCATAT207.031889E-445.017
CGACCCC453.8380676E-1045.019
TCGATGG700.045.01
TTATCGG253.889647E-545.01
AATGCGG502.1827873E-1145.01
CTACGTC207.031889E-445.013
AGTTGCG207.031889E-445.013