FastQCFastQC Report
Thu 26 May 2016
SRR1043397_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043397_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences861574
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387874.501876797582099No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40320.4679806957962984No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14890.17282322818469453No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT10340.12001290661045945No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTTCG253.8899903E-545.044
GATCGGA207.032303E-445.08
GTCGACA207.032303E-445.013
ACGTTGG253.8899903E-545.01
CACATCG253.8899903E-545.016
TCGATTG253.8899903E-545.013
CTGGTCG207.032303E-445.043
ATCGTAA253.8899903E-545.013
CGTTAGC207.032303E-445.018
TGGCATA207.032303E-445.025
CGTTACT253.8899903E-545.028
AAACGCG207.032303E-445.020
AACGGAC502.1827873E-1145.014
CCCGTAC207.032303E-445.036
CATACGT406.8121153E-945.012
ACGTCTT207.032303E-445.028
CGTAAGT207.032303E-445.038
CGTAAGG207.032303E-445.01
GCGTCAA253.8899903E-545.022
AATACGT207.032303E-445.021