##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043397_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	861574
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5211531452899	31.0	31.0	33.0	30.0	34.0
2	30.60495906329578	31.0	31.0	34.0	26.0	34.0
3	31.494941815792956	31.0	31.0	34.0	28.0	34.0
4	35.09041359186791	37.0	35.0	37.0	32.0	37.0
5	35.39363769101667	37.0	35.0	37.0	33.0	37.0
6	35.83624389779636	37.0	35.0	37.0	35.0	37.0
7	33.96267180764508	37.0	35.0	37.0	32.0	37.0
8	34.881487835055374	37.0	35.0	37.0	32.0	37.0
9	36.78630042225044	39.0	37.0	39.0	32.0	39.0
10	36.635597174473695	39.0	35.0	39.0	32.0	39.0
11	36.427086936235305	38.0	35.0	39.0	32.0	39.0
12	36.34748843395924	39.0	35.0	39.0	32.0	39.0
13	36.41922458198599	39.0	35.0	39.0	32.0	39.0
14	37.72268545708204	40.0	36.0	41.0	33.0	41.0
15	37.84585653698928	40.0	36.0	41.0	33.0	41.0
16	37.80790042410751	40.0	36.0	41.0	33.0	41.0
17	37.7989238301063	40.0	36.0	41.0	33.0	41.0
18	37.52113457462737	39.0	36.0	41.0	33.0	41.0
19	37.254640924633286	38.0	35.0	41.0	33.0	41.0
20	36.960411990148266	38.0	35.0	41.0	32.0	41.0
21	36.86158704881995	38.0	35.0	40.0	32.0	41.0
22	36.77154370953627	38.0	35.0	40.0	32.0	41.0
23	36.70243647092415	38.0	35.0	40.0	32.0	41.0
24	36.554033663968504	38.0	35.0	40.0	32.0	41.0
25	36.46460083521555	37.0	35.0	40.0	32.0	41.0
26	36.217031851007576	37.0	35.0	40.0	31.0	41.0
27	36.14402941592945	37.0	35.0	40.0	31.0	41.0
28	36.03054293653244	37.0	35.0	40.0	31.0	41.0
29	35.921036382249234	37.0	35.0	40.0	31.0	41.0
30	35.74562602864061	37.0	35.0	40.0	30.0	41.0
31	35.308074524068736	37.0	34.0	40.0	29.0	41.0
32	35.11031437810333	37.0	34.0	40.0	27.0	41.0
33	34.93702108002331	37.0	34.0	40.0	25.0	41.0
34	34.72878127705803	37.0	34.0	40.0	24.0	41.0
35	34.512050038650194	37.0	34.0	40.0	23.0	41.0
36	34.32769442903337	37.0	34.0	40.0	22.0	41.0
37	34.22294312502466	37.0	34.0	40.0	21.0	41.0
38	34.12058047248408	36.0	34.0	40.0	21.0	41.0
39	33.98671269095864	36.0	33.0	40.0	20.0	41.0
40	33.95014705643392	36.0	33.0	40.0	20.0	41.0
41	33.836413355091956	36.0	33.0	40.0	19.0	41.0
42	33.724774656616844	36.0	33.0	40.0	18.0	41.0
43	33.5404585096579	35.0	33.0	40.0	18.0	41.0
44	33.35526722022716	35.0	33.0	40.0	18.0	41.0
45	33.216278578508636	35.0	33.0	40.0	17.0	41.0
46	33.20950260801742	35.0	33.0	40.0	18.0	41.0
47	33.16454419469483	35.0	33.0	40.0	18.0	41.0
48	33.09622736990671	35.0	33.0	40.0	17.0	41.0
49	32.98065169097489	35.0	33.0	40.0	15.0	41.0
50	32.87720845800825	35.0	33.0	40.0	15.0	41.0
51	32.39397312360865	35.0	31.0	39.0	14.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	4.0
13	10.0
14	16.0
15	58.0
16	117.0
17	234.0
18	515.0
19	978.0
20	1647.0
21	2629.0
22	4055.0
23	6502.0
24	10386.0
25	15601.0
26	19709.0
27	21145.0
28	21395.0
29	21274.0
30	23189.0
31	27066.0
32	32695.0
33	42284.0
34	71853.0
35	98674.0
36	84019.0
37	81383.0
38	108784.0
39	165265.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.764124729854892	18.066469043866228	24.93401611469241	31.23539011158647
2	31.665881282397102	20.255137689856006	28.87308577092623	19.205895256820657
3	22.289089503629402	21.90328398953543	37.56032563656749	18.247300870267676
4	22.98154308277641	24.77512088340642	34.1848755881677	18.058460445649473
5	19.902295101755623	31.25500537388547	32.67171479176484	16.170984732594064
6	79.32934373599946	1.9965783554285528	17.412317456190646	1.2617604523813393
7	76.17685770461968	6.396316509086858	15.936065851569337	1.4907599347241212
8	75.26677917393052	3.9146956616610993	18.691720037977007	2.1268051264313916
9	38.6960377170156	27.43014529222098	24.429706560318674	9.444110430444743
10	28.15741886361473	22.290482303319273	37.835751775239274	11.716347057826722
11	26.741986178784412	21.387483837720264	37.788628719065336	14.081901264429986
12	18.530271340592915	29.5256124256303	37.55324557147732	14.390870662299465
13	15.034924452223489	31.495147253747213	37.80023538314759	15.669692910881711
14	14.758105513861839	23.539359358569317	46.82998790585602	14.872547221712818
15	16.31165750127093	23.16167850933292	44.43460457256138	16.09205941683477
16	16.732166940970828	21.9418181142885	37.73570233085028	23.59031261389039
17	18.534914006225815	21.858714399459593	41.35268705880168	18.25368453551291
18	20.94585026939067	24.026026783538036	38.616416001411366	16.411706945659922
19	22.54385578023478	24.19548407913888	36.262816658812824	16.99784348181352
20	18.58772432780005	26.429418715049437	38.414227913098586	16.56862904405193
21	18.372536775715144	23.677478661148086	36.859283125999625	21.090701437137145
22	15.28539626311843	26.445203778201293	37.993254206835395	20.27614575184488
23	15.054771847804135	23.98993005824224	44.75576096771722	16.1995371262364
24	15.388347373527985	24.398252500655776	42.79771673704174	17.4156833887745
25	15.327412387096176	29.216991227683287	37.77690598834227	17.67869039687827
26	15.589142662150902	28.469986327349712	37.30486296011718	18.63600805038221
27	15.438139962440836	26.503933498457478	41.764375433799074	16.29355110530262
28	15.05825384702881	23.946637201215452	40.56621950058846	20.428889451167283
29	16.820841854559212	27.25186693191763	38.85110274915446	17.076188464368702
30	21.111129165921906	26.32519087159083	35.76639963601502	16.797280326472247
31	20.861818021435187	25.396657745010877	37.32157655639562	16.419947677158316
32	20.753759978829443	27.213564940446204	34.88173969966596	17.15093538105839
33	17.11994558795878	26.821491827747817	36.36611596914484	19.692446615148555
34	16.32430876512058	26.844124822708203	37.392493273938165	19.43907313823305
35	22.009484965888014	27.19313721166145	34.22828451183532	16.569093310615223
36	18.1684916211492	31.05386188534009	34.22631137894133	16.551335114569383
37	17.053787602689958	29.634366868080974	35.052705861597495	18.25913966763157
38	17.65153080292581	29.72803264722473	34.16734952540351	18.453087024445956
39	19.775666396618284	27.552595598288715	33.131338689421916	19.54039931567109
40	16.588824639555046	26.710880319043984	34.568824036008515	22.131471005392456
41	19.069168753931756	27.27380352703308	31.829419179315998	21.827608539719165
42	18.38483983964233	27.163192018329248	33.50298407333555	20.94898406869288
43	17.8315501628415	28.711869206823792	34.4758546567097	18.980725973625017
44	16.309568301736125	32.020116670187356	32.42356431368635	19.246750714390174
45	18.71296023324752	29.765406105569575	33.041735242706956	18.47989841847595
46	18.09722670368419	28.62493529284774	32.985326855267225	20.292511148200852
47	17.8093814344444	27.925053448688097	35.08973112001987	19.17583399684763
48	18.940682982541258	25.917100562458938	35.12037271319701	20.021843741802794
49	18.763797421927777	29.603841341544662	33.00819198350925	18.624169253018312
50	17.422995587146318	28.114938473073696	33.90353005081398	20.55853588896601
51	17.18169304087635	26.306736275700054	35.581273343903135	20.93029733952046
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	440.0
1	3948.0
2	7456.0
3	29370.0
4	51284.0
5	31016.0
6	10748.0
7	10613.0
8	10478.0
9	11321.5
10	12165.0
11	13123.5
12	14082.0
13	13973.0
14	13864.0
15	12750.0
16	11636.0
17	10635.0
18	9634.0
19	9150.5
20	8667.0
21	8289.5
22	7912.0
23	7597.5
24	7283.0
25	8396.5
26	9534.0
27	9558.0
28	11138.5
29	12719.0
30	14003.0
31	15287.0
32	16790.5
33	18294.0
34	20671.5
35	23049.0
36	26024.0
37	28999.0
38	30879.0
39	32759.0
40	38285.0
41	43811.0
42	51248.5
43	58686.0
44	65018.0
45	71350.0
46	71521.0
47	71692.0
48	66916.5
49	62141.0
50	57387.0
51	52633.0
52	46877.0
53	41121.0
54	36751.0
55	32381.0
56	30223.0
57	28065.0
58	25265.0
59	22465.0
60	19088.5
61	15712.0
62	13757.5
63	11803.0
64	9978.5
65	8154.0
66	7473.0
67	6792.0
68	5515.0
69	4238.0
70	3853.0
71	3468.0
72	3677.5
73	3887.0
74	3517.5
75	2534.5
76	1921.0
77	1577.5
78	1234.0
79	878.0
80	522.0
81	393.0
82	264.0
83	214.0
84	164.0
85	107.5
86	51.0
87	34.0
88	17.0
89	12.0
90	7.0
91	7.0
92	7.0
93	10.5
94	14.0
95	8.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	861574.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.33788913317736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.94313121773429	27.896836187981673
2	9.38948437893495	6.636091160010079
3	3.4123614286607844	3.6175694954503657
4	1.7196282724195822	2.4307213296416204
5	1.034015807694736	1.8269967987134719
6	0.6786496631344698	1.4389227933668436
7	0.5107656750879084	1.2634566559502296
8	0.3919880580825927	1.108162443044172
9	0.304442650075838	0.9682524554211607
>10	2.798521479753274	22.27356579580154
>50	0.6326128948529716	15.54313023553124
>100	0.18064915155524383	8.984599745866774
>500	0.002385932190352263	0.5503282645672531
>1k	0.0010225423672938268	0.7895385804855208
>5k	0.0	0.0
>10k+	3.4084745576460897E-4	4.671828058168099
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38787	4.501876797582099	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4032	0.4679806957962984	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1489	0.17282322818469453	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	1034	0.12001290661045945	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08380011467384113	0.0
2	0.0	0.0	0.0	0.2744976055451998	0.0
3	0.0	0.0	0.0	0.4511510328770367	0.0
4	0.0	0.0	0.0	0.5804492707532957	0.0
5	0.0	0.0	0.0	1.2582784531566644	0.0
6	0.0	0.0	0.0	1.4314498812638263	0.0
7	0.0	0.0	0.0	2.009345685919027	0.0
8	0.0	0.0	0.0	2.622873949306734	0.0
9	0.0	0.0	0.0	3.149584365359215	0.0
10	0.0	0.0	0.0	3.4991770875165686	0.0
11	0.0	0.0	0.0	3.9281593919965085	0.0
12	0.0	0.0	0.0	4.288082045187065	0.0
13	0.0	0.0	0.0	4.457191140865439	0.0
14	0.0	0.0	0.0	4.587185778586633	0.0
15	0.0	0.0	0.0	5.0412384774842325	0.0
16	0.0	0.0	0.0	5.591974688186969	0.0
17	0.0	0.0	0.0	6.299632997281719	0.0
18	0.0	0.0	0.0	6.675224646983312	0.0
19	0.0	0.0	0.0	7.079020490404771	0.0
20	0.0	0.0	0.0	7.532376789457435	0.0
21	0.0	0.0	0.0	8.004187684400875	0.0
22	0.0	0.0	0.0	8.49387284203098	0.0
23	1.1606664082249465E-4	0.0	0.0	8.903588084134386	0.0
24	0.0019731328939824088	0.0	0.0	9.244127608307586	0.0
25	0.0019731328939824088	0.0	0.0	9.586640265374768	0.0
26	0.0019731328939824088	0.0	0.0	9.937741853862814	0.0
27	0.0019731328939824088	0.0	0.0	10.30137863955969	0.0
28	0.0019731328939824088	0.0	0.0	10.669774157530288	0.0
29	0.0019731328939824088	0.0	0.0	11.140192252783859	0.0
30	0.0019731328939824088	0.0	0.0	11.566505024524881	0.0
31	0.0019731328939824088	0.0	0.0	11.927124077560372	0.0
32	0.0019731328939824088	0.0	0.0	12.312349258450231	0.0
33	0.0019731328939824088	0.0	0.0	12.699431505593251	0.0
34	0.0019731328939824088	0.0	0.0	13.143734606661761	0.0
35	0.0019731328939824088	0.0	0.0	13.564940446206593	0.0
36	0.0019731328939824088	0.0	0.0	13.98951221833528	0.0
37	0.0019731328939824088	0.0	0.0	14.423717521652232	0.0
38	0.0019731328939824088	0.0	0.0	14.855601492152735	0.0
39	0.0019731328939824088	0.0	0.0	15.308029258078818	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTTCG	25	3.8899903E-5	45.0	44
GATCGGA	20	7.032303E-4	45.0	8
GTCGACA	20	7.032303E-4	45.0	13
ACGTTGG	25	3.8899903E-5	45.0	1
CACATCG	25	3.8899903E-5	45.0	16
TCGATTG	25	3.8899903E-5	45.0	13
CTGGTCG	20	7.032303E-4	45.0	43
ATCGTAA	25	3.8899903E-5	45.0	13
CGTTAGC	20	7.032303E-4	45.0	18
TGGCATA	20	7.032303E-4	45.0	25
CGTTACT	25	3.8899903E-5	45.0	28
AAACGCG	20	7.032303E-4	45.0	20
AACGGAC	50	2.1827873E-11	45.0	14
CCCGTAC	20	7.032303E-4	45.0	36
CATACGT	40	6.8121153E-9	45.0	12
ACGTCTT	20	7.032303E-4	45.0	28
CGTAAGT	20	7.032303E-4	45.0	38
CGTAAGG	20	7.032303E-4	45.0	1
GCGTCAA	25	3.8899903E-5	45.0	22
AATACGT	20	7.032303E-4	45.0	21
>>END_MODULE