FastQCFastQC Report
Thu 26 May 2016
SRR1043396_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043396_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1229049
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT543534.4223623305498805No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57020.4639359374605894No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22530.1833124635388825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGG453.8562575E-1045.0000041
TACCGTC351.212029E-745.00000418
CTAAGCG253.890964E-545.022
AATGCGC207.0334785E-445.040
CCGTAGA207.0334785E-445.039
CGGATAG207.0334785E-445.029
CTCGCTA253.890964E-545.09
ACGTCCG800.045.039
CGACTGG253.890964E-545.01
CGACTCA253.890964E-545.021
CGGTACG207.0334785E-445.021
ATACCGT207.0334785E-445.016
GTACGTC207.0334785E-445.023
TACGTCG207.0334785E-445.024
CTAGCGG3150.044.2857131
CGTTTTT140350.044.18241
TTCACGG1950.042.6923071
ACGGGAT8550.042.3684234
CACGTCC850.042.3529438
CCACGTC850.042.3529437