##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043396_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1229049
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.530171701860546	31.0	31.0	33.0	30.0	34.0
2	30.576322831717857	31.0	31.0	34.0	26.0	34.0
3	31.50143647649524	31.0	31.0	34.0	28.0	34.0
4	35.11016810558407	37.0	35.0	37.0	32.0	37.0
5	35.42559735209906	37.0	35.0	37.0	33.0	37.0
6	35.834300341158084	37.0	35.0	37.0	35.0	37.0
7	33.78930132159092	37.0	35.0	37.0	32.0	37.0
8	34.788331466035935	37.0	35.0	37.0	31.0	37.0
9	36.7535061661496	39.0	37.0	39.0	32.0	39.0
10	36.64951763517972	39.0	35.0	39.0	32.0	39.0
11	36.530051283553384	39.0	35.0	39.0	32.0	39.0
12	36.48252022498696	39.0	35.0	39.0	32.0	39.0
13	36.5385139241804	39.0	35.0	39.0	32.0	39.0
14	37.86526005065705	40.0	36.0	41.0	33.0	41.0
15	37.98498595255356	40.0	36.0	41.0	33.0	41.0
16	37.93772258062941	40.0	36.0	41.0	33.0	41.0
17	37.89132898688335	40.0	36.0	41.0	33.0	41.0
18	37.672999205076444	39.0	36.0	41.0	33.0	41.0
19	37.42723845835276	39.0	36.0	41.0	33.0	41.0
20	37.15089390252138	39.0	35.0	41.0	33.0	41.0
21	37.05096786214382	38.0	35.0	41.0	32.0	41.0
22	36.967990698499406	38.0	35.0	41.0	32.0	41.0
23	36.90355225869758	38.0	35.0	40.0	32.0	41.0
24	36.770564883906175	38.0	35.0	40.0	32.0	41.0
25	36.66986507454137	38.0	35.0	40.0	32.0	41.0
26	36.38817980405989	38.0	35.0	40.0	31.0	41.0
27	36.305181485848	38.0	35.0	40.0	31.0	41.0
28	36.21946806026448	37.0	35.0	40.0	31.0	41.0
29	36.16771910639852	37.0	35.0	40.0	31.0	41.0
30	35.97144947028149	37.0	35.0	40.0	30.0	41.0
31	35.588039207549905	37.0	35.0	40.0	29.0	41.0
32	35.436840191074566	38.0	35.0	40.0	28.0	41.0
33	35.31415997246652	38.0	35.0	40.0	27.0	41.0
34	35.11788219997738	38.0	35.0	40.0	25.0	41.0
35	34.91916514313099	38.0	35.0	40.0	24.0	41.0
36	34.762160011521104	38.0	34.0	40.0	23.0	41.0
37	34.67803236486096	37.0	34.0	40.0	23.0	41.0
38	34.59207891630032	37.0	34.0	40.0	23.0	41.0
39	34.450155364025356	37.0	34.0	40.0	22.0	41.0
40	34.37672297849801	37.0	34.0	40.0	22.0	41.0
41	34.27688643821361	37.0	34.0	40.0	21.0	41.0
42	34.176675624812354	37.0	34.0	40.0	21.0	41.0
43	34.02939183059422	36.0	34.0	40.0	20.0	41.0
44	33.8383319135364	36.0	33.0	40.0	19.0	41.0
45	33.66764384495655	36.0	33.0	40.0	19.0	41.0
46	33.665001964933865	36.0	33.0	40.0	20.0	41.0
47	33.647414382990426	36.0	33.0	40.0	20.0	41.0
48	33.58790658468458	36.0	33.0	40.0	20.0	41.0
49	33.49034253312927	35.0	33.0	40.0	18.0	41.0
50	33.38022650032668	35.0	33.0	40.0	18.0	41.0
51	32.94204136694306	35.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	7.0
13	14.0
14	33.0
15	56.0
16	136.0
17	319.0
18	659.0
19	1234.0
20	2157.0
21	3378.0
22	5379.0
23	8336.0
24	13345.0
25	20176.0
26	25870.0
27	27778.0
28	27726.0
29	28265.0
30	30860.0
31	36502.0
32	44871.0
33	57680.0
34	97682.0
35	135250.0
36	122130.0
37	117314.0
38	159914.0
39	261861.0
40	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.05567393976969	18.527577012796073	25.935662451212277	29.481086596221957
2	30.616761414719836	21.36700815020394	28.989405629881315	19.026824805194913
3	22.025403380988067	21.87878595564538	36.12532942136563	19.97048124200093
4	22.85832379343704	25.187523036103528	33.29468556583179	18.659467604627643
5	20.366478472379864	32.25908812423264	30.872894408603724	16.501538994783772
6	81.13289217923777	1.8488278335526087	15.842330126789086	1.1759498604205365
7	77.47388427963409	6.948136323287354	14.354757214724556	1.223222182353999
8	77.47494200800782	3.9371904618937084	16.632127767078448	1.9557397630200262
9	36.187003121925976	32.04184698901346	22.240529059459792	9.530620829600773
10	25.586449360440472	23.80149204791672	38.64972023084515	11.962338360797657
11	25.0216224088706	21.581727010070388	38.50993735807116	14.886713222987854
12	18.221079875578596	29.42518971985657	37.118861819178896	15.234868585385936
13	15.464639733647722	32.2744658675122	36.419540636703665	15.841353762136414
14	14.101797405961843	25.622168033984	44.32077158843952	15.955262971614639
15	16.377866138778842	23.788067033942504	42.98152473985984	16.852542087418808
16	17.394261742208812	23.706133766839237	36.41148562831913	22.488118862632817
17	17.144231027404114	24.17967062338442	40.94499080183133	17.73110754738013
18	20.157861891592603	25.855112367366967	36.72026094972617	17.26676479131426
19	21.384745441394116	26.06698349699646	36.046732066825655	16.501538994783772
20	17.78928260793508	27.909302232864597	37.287772904090886	17.01364225510944
21	18.310824059903226	24.149566046593748	36.64971860357073	20.889891289932297
22	15.157003504335464	26.609760880160188	37.79304161184786	20.440194003656487
23	14.470456426066008	24.182518353621376	44.55225137484348	16.794773845469138
24	15.70246589029404	26.629206809492544	40.2602337254251	17.40809357478831
25	16.582902715839644	29.93119070110305	36.51384118940742	16.97206539364989
26	15.007212893871602	30.33019838916105	36.72953641392654	17.933052303040807
27	15.019986998077375	27.69401382695076	40.43337572383201	16.852623451139863
28	14.616911123966577	25.853403729224787	39.31055637326095	20.21912877354768
29	15.615488072485311	28.42677549877995	38.57022787537356	17.387508553361176
30	19.365460612229455	28.85605049107074	35.568150659575004	16.2103382371248
31	20.146633698086895	27.865772642099707	34.92684181021261	17.060751849600788
32	19.05603438105397	29.461233848284323	34.51302592492244	16.969705845739266
33	16.55442541347009	28.380560905220214	34.96394366701409	20.101070014295605
34	15.997328015400525	28.38381545406245	35.591176592633815	20.02767993790321
35	20.716423836641177	27.871630830015725	33.88261981418154	17.529325519161564
36	16.794448390584915	30.78249931451065	34.45867495925712	17.964377335647317
37	16.819833871554348	30.307660638428572	34.19684650489931	18.675658985117764
38	17.377663543113414	30.81797389689101	33.032775747752936	18.771586812242635
39	19.904657991666728	28.065927395897155	32.896816969868574	19.132597642567546
40	16.663127344800735	27.83265760762996	34.13785780713381	21.36635724043549
41	18.505771535553098	28.20294390215524	31.94063052001995	21.350654042271707
42	18.100987023300128	28.12393972900999	32.99827753002525	20.776795717664633
43	17.583757848547943	28.972319248459584	33.99229811016485	19.451624792827626
44	16.15598727145948	32.98485251605103	31.42543543829416	19.433724774195333
45	18.600641634304246	30.278044243964235	32.44118013195568	18.680133989775836
46	17.568380105268382	30.464285801461127	31.44455591274229	20.5227781805282
47	17.454959078116495	29.011292470845344	33.51103170011936	20.0227167509188
48	18.332954992030423	27.650646963627977	34.31140662414599	19.704991420195615
49	17.94834868259931	30.270151963021817	32.70919222911373	19.072307125265144
50	16.873615291172282	29.600203083847752	32.81008324322301	20.716098381756954
51	16.647342782915896	28.14216520252651	33.951372158473745	21.25911985608385
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	941.0
1	5718.5
2	10496.0
3	40442.0
4	70388.0
5	42445.5
6	14503.0
7	14189.5
8	13876.0
9	15354.0
10	16832.0
11	18366.0
12	19900.0
13	19629.0
14	19358.0
15	17552.0
16	15746.0
17	14376.5
18	13007.0
19	12026.0
20	11045.0
21	11258.0
22	11471.0
23	11000.0
24	10529.0
25	11850.5
26	15113.5
27	17055.0
28	18766.5
29	20478.0
30	23409.5
31	26341.0
32	30204.5
33	34068.0
34	39414.0
35	44760.0
36	46705.5
37	48651.0
38	49963.5
39	51276.0
40	58293.0
41	65310.0
42	72277.5
43	79245.0
44	83282.5
45	87320.0
46	86088.0
47	84856.0
48	82956.5
49	81057.0
50	78255.0
51	75453.0
52	72098.0
53	68743.0
54	60750.0
55	52757.0
56	46782.5
57	40808.0
58	36328.0
59	31848.0
60	27074.5
61	22301.0
62	18650.5
63	15000.0
64	12754.5
65	10509.0
66	8908.5
67	7308.0
68	6419.5
69	5531.0
70	4914.0
71	4297.0
72	4715.0
73	5133.0
74	4222.5
75	2814.0
76	2316.0
77	1694.0
78	1072.0
79	805.5
80	539.0
81	388.0
82	237.0
83	178.5
84	120.0
85	82.5
86	45.0
87	32.0
88	19.0
89	13.0
90	7.0
91	7.5
92	8.0
93	5.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1229049.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.72937980385432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.18042874526743	25.915263254358823
2	8.885015333892127	5.816020828520499
3	3.2082625798422684	3.1501333345845337
4	1.706134871902001	2.2336294487632373
5	1.0385561893137356	1.699564998384644
6	0.7360229380802189	1.4453744570865743
7	0.5141708423917585	1.1779944949295307
8	0.3887725501710383	1.017942756148874
9	0.3283069821523033	0.9670755520007962
>10	3.007995206971467	22.168291507729364
>50	0.773037987569083	17.65468444563761
>100	0.2291893562108771	10.69382433301101
>500	0.002823161162030674	0.6437781871634164
>1k	7.699530441901837E-4	0.37178526309828824
>5k	2.5665101473006124E-4	0.4789696272450157
>10k+	2.5665101473006124E-4	4.5656675113378355
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	54353	4.4223623305498805	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5702	0.4639359374605894	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2253	0.1833124635388825	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05939551637078749	0.0
2	0.0	0.0	0.0	0.19218110913397268	0.0
3	0.0	0.0	0.0	0.3157726014178442	0.0
4	0.0	0.0	0.0	0.3996585978264496	0.0
5	0.0	0.0	0.0	0.8564345278341221	0.0
6	0.0	0.0	0.0	0.9858028443129607	0.0
7	0.0	0.0	0.0	1.3805796188760577	0.0
8	0.0	0.0	0.0	1.8197809851356619	0.0
9	0.0	0.0	0.0	2.1686686210232464	0.0
10	0.0	0.0	0.0	2.4085288706959607	0.0
11	0.0	0.0	0.0	2.697939626491702	0.0
12	0.0	0.0	0.0	2.953340346886088	0.0
13	0.0	0.0	0.0	3.0690395582275403	0.0
14	0.0	0.0	0.0	3.1602482895311743	0.0
15	0.0	0.0	0.0	3.4696745207066604	0.0
16	0.0	0.0	0.0	3.8727503948174564	0.0
17	0.0	0.0	0.0	4.355155896957729	0.0
18	0.0	0.0	0.0	4.618367534573479	0.0
19	0.0	0.0	0.0	4.915101025264249	0.0
20	0.0	0.0	0.0	5.230873626682094	0.0
21	0.0	0.0	0.0	5.566254884874403	0.0
22	0.0	0.0	0.0	5.9264520779887535	0.0
23	8.136372105587328E-5	0.0	0.0	6.234901944511569	0.0
24	4.0681860527936643E-4	0.0	0.0	6.498764491895766	0.0
25	4.0681860527936643E-4	0.0	0.0	6.750747936005806	0.0
26	4.0681860527936643E-4	0.0	0.0	7.006555475005472	0.0
27	4.0681860527936643E-4	0.0	0.0	7.269360294015942	0.0
28	4.0681860527936643E-4	0.0	0.0	7.566500603311992	0.0
29	4.0681860527936643E-4	0.0	0.0	7.916852786178582	0.0
30	4.0681860527936643E-4	0.0	0.0	8.23669357364922	0.0
31	4.0681860527936643E-4	0.0	0.0	8.51528295454453	0.0
32	4.0681860527936643E-4	0.0	0.0	8.810795989419463	0.0
33	4.0681860527936643E-4	0.0	0.0	9.126568590837305	0.0
34	4.0681860527936643E-4	0.0	0.0	9.492786699309791	0.0
35	4.0681860527936643E-4	0.0	0.0	9.827272956570487	0.0
36	4.0681860527936643E-4	0.0	0.0	10.158667392431058	0.0
37	4.0681860527936643E-4	0.0	0.0	10.516179582750565	0.0
38	8.136372105587329E-4	0.0	0.0	10.864416308869703	0.0
39	8.136372105587329E-4	0.0	0.0	11.237468969910882	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACGG	45	3.8562575E-10	45.000004	1
TACCGTC	35	1.212029E-7	45.000004	18
CTAAGCG	25	3.890964E-5	45.0	22
AATGCGC	20	7.0334785E-4	45.0	40
CCGTAGA	20	7.0334785E-4	45.0	39
CGGATAG	20	7.0334785E-4	45.0	29
CTCGCTA	25	3.890964E-5	45.0	9
ACGTCCG	80	0.0	45.0	39
CGACTGG	25	3.890964E-5	45.0	1
CGACTCA	25	3.890964E-5	45.0	21
CGGTACG	20	7.0334785E-4	45.0	21
ATACCGT	20	7.0334785E-4	45.0	16
GTACGTC	20	7.0334785E-4	45.0	23
TACGTCG	20	7.0334785E-4	45.0	24
CTAGCGG	315	0.0	44.285713	1
CGTTTTT	14035	0.0	44.1824	1
TTCACGG	195	0.0	42.692307	1
ACGGGAT	855	0.0	42.368423	4
CACGTCC	85	0.0	42.35294	38
CCACGTC	85	0.0	42.35294	37
>>END_MODULE