FastQCFastQC Report
Thu 26 May 2016
SRR1043395_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043395_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences804207
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT422415.252503397757045No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46080.5729868056358625No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18040.22432035533140102No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT11690.14536058502350763No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT11620.1444901623586962No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8220.10221249006785565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTACT302.1646847E-645.0000048
TTTAGCG253.8897575E-545.023
AACCGTA207.0320227E-445.036
AACCGGT253.8897575E-545.035
CGAACCT207.0320227E-445.023
CTCGTCG207.0320227E-445.039
TTTACGG502.1827873E-1145.01
CGCGGAT207.0320227E-445.022
CCCTAAG351.2113196E-745.018
TTATGTC207.0320227E-445.014
TAGGTCG207.0320227E-445.01
CATTGCG207.0320227E-445.012
CGCATAC253.8897575E-545.023
CAACCCG253.8897575E-545.022
GCACGCT253.8897575E-545.023
ATTGTCG253.8897575E-545.035
TACGGTA207.0320227E-445.044
TTATCGG207.0320227E-445.01
ATCCGGA253.8897575E-545.010
CCGTATG207.0320227E-445.034