##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043395_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	804207
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.506605886295443	31.0	31.0	33.0	30.0	34.0
2	30.552337893104635	31.0	31.0	34.0	26.0	34.0
3	31.46406211336136	31.0	31.0	34.0	28.0	34.0
4	35.081022672023494	37.0	35.0	37.0	32.0	37.0
5	35.39360761594963	37.0	35.0	37.0	33.0	37.0
6	35.79486500366199	37.0	35.0	37.0	35.0	37.0
7	33.76015752163311	37.0	35.0	37.0	32.0	37.0
8	34.770632436673644	37.0	35.0	37.0	31.0	37.0
9	36.744677676269916	39.0	37.0	39.0	32.0	39.0
10	36.6429638140429	39.0	35.0	39.0	32.0	39.0
11	36.188376873118486	38.0	35.0	39.0	32.0	39.0
12	35.96848696915098	38.0	35.0	39.0	31.0	39.0
13	36.057059936061236	38.0	35.0	39.0	31.0	39.0
14	37.42503857837597	39.0	35.0	41.0	32.0	41.0
15	37.6240234168566	40.0	35.0	41.0	33.0	41.0
16	37.59644469645253	40.0	35.0	41.0	33.0	41.0
17	37.59231391917753	39.0	36.0	41.0	33.0	41.0
18	37.30299910346466	39.0	36.0	40.0	33.0	41.0
19	37.00642123234441	38.0	35.0	40.0	32.0	41.0
20	36.69749330707144	38.0	35.0	40.0	32.0	41.0
21	36.58536172900758	37.0	35.0	40.0	32.0	41.0
22	36.50825595897574	37.0	35.0	40.0	32.0	41.0
23	36.411058346918146	37.0	35.0	40.0	32.0	41.0
24	36.26420063491116	37.0	35.0	40.0	32.0	41.0
25	36.17747420751125	36.0	35.0	40.0	31.0	41.0
26	35.88955455498398	36.0	35.0	40.0	31.0	41.0
27	35.823789148813674	36.0	35.0	40.0	31.0	41.0
28	35.71691616710623	36.0	35.0	40.0	31.0	41.0
29	35.694643294574654	36.0	35.0	40.0	31.0	41.0
30	35.50521569695365	35.0	35.0	40.0	30.0	41.0
31	35.06645925738025	35.0	34.0	40.0	29.0	41.0
32	34.92183480123899	35.0	34.0	40.0	27.0	41.0
33	34.80446700911581	35.0	34.0	40.0	25.0	41.0
34	34.60747046469379	35.0	34.0	40.0	24.0	41.0
35	34.37000548366279	35.0	34.0	40.0	23.0	41.0
36	34.183537323102136	35.0	34.0	40.0	23.0	41.0
37	34.111278563852345	35.0	33.0	40.0	22.0	41.0
38	34.03572463308576	35.0	34.0	40.0	22.0	41.0
39	33.9023696635319	35.0	33.0	40.0	21.0	41.0
40	33.85626959228159	35.0	33.0	40.0	21.0	41.0
41	33.78700757392065	35.0	33.0	40.0	21.0	41.0
42	33.69080473062284	35.0	33.0	40.0	21.0	41.0
43	33.538734430314584	35.0	33.0	40.0	20.0	41.0
44	33.33641089918392	35.0	33.0	40.0	18.0	41.0
45	33.1772404368527	35.0	33.0	40.0	18.0	41.0
46	33.18998093774364	35.0	33.0	40.0	19.0	41.0
47	33.19289063636601	35.0	33.0	40.0	19.0	41.0
48	33.14635411032234	35.0	33.0	40.0	18.0	41.0
49	33.05207987495757	35.0	33.0	40.0	18.0	41.0
50	32.93758447762827	35.0	33.0	39.0	18.0	41.0
51	32.522937502409206	35.0	32.0	39.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	9.0
14	15.0
15	54.0
16	100.0
17	256.0
18	536.0
19	932.0
20	1660.0
21	2511.0
22	3938.0
23	5951.0
24	9284.0
25	13857.0
26	17528.0
27	19531.0
28	19490.0
29	20134.0
30	22104.0
31	25924.0
32	32341.0
33	42609.0
34	76133.0
35	108453.0
36	82411.0
37	69398.0
38	88211.0
39	140767.0
40	67.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.96943324293372	18.498844203047227	24.72970267605231	30.802019877966742
2	30.953473421643928	21.59879235072562	29.500862340168638	17.94687188746181
3	20.89051699375907	21.499439820842145	38.118792798371565	19.491250387027222
4	22.110352185444793	25.868215521625654	34.52568803803001	17.49574425489955
5	19.849491486644606	31.48070086432971	32.34254364858799	16.3272640004377
6	79.45516515026604	2.1688445885201197	17.0676206499073	1.3083696113065417
7	76.12480368860255	7.159475110263899	15.464550793514606	1.251170407618934
8	75.70240000397908	3.832844031449614	18.172187011552996	2.292568953018315
9	43.523744508565585	22.698509214667368	23.75545102193838	10.02229525482867
10	34.248644938430026	22.06036505526562	33.831961174175305	9.859028832129042
11	32.1989239088941	20.453937854308656	35.1082494929788	12.238888743818444
12	18.767929152568925	33.978565220148546	33.04621820004054	14.20728742724199
13	13.562179886521752	35.52045679781449	34.25784654945804	16.659516766205716
14	12.085570008716662	24.056368571773188	47.62231614497263	16.235745274537525
15	14.347301130181659	20.910039330669843	46.69631077570824	18.04634876344026
16	15.553955635800234	23.15112900036931	34.43678058012427	26.858134783706184
17	17.828743097237403	23.16791572319067	41.10098519411047	17.902355985461455
18	21.920351352325955	24.937982385132187	34.41153832284474	18.730127939697116
19	23.25607710452657	24.67648254740384	35.598546145457576	16.468894202612013
20	17.40857764232343	30.047487773670213	36.14106815782504	16.40286642618132
21	19.453076135870493	25.740387735993348	32.740078114216864	22.066458013919302
22	15.014417929712126	29.431228526983727	34.12181192155751	21.43254162174664
23	14.203805736582744	24.487849521329707	46.788202539893334	14.520142202194211
24	14.043523620162471	26.78874966271122	42.13952377932547	17.02820293780084
25	14.035192431799276	32.00171100226683	35.934777986264734	18.028318579669165
26	13.782148128529098	34.01910204710976	35.486385967791875	16.712363856569265
27	14.295075770292973	29.303773779636337	40.81648132881211	15.584669121258582
28	12.403398627467805	25.478390513885106	40.713149723889494	21.405061134757595
29	14.202810967822959	31.636382175235976	37.22959387321921	16.931212983721853
30	21.202003961666584	29.291214824044058	33.56822310673744	15.938558107551911
31	20.71494030765711	27.310630223313154	34.92906677012262	17.04536269890712
32	19.126916328756153	30.802517262346633	32.95009866862636	17.12046774027085
33	15.554950404560019	29.669475644952108	33.241690261338185	21.533883689149686
34	15.830252658830377	28.790473099587544	35.52903667836763	19.850237563214446
35	23.26291613975009	28.075856091777368	31.131288337455405	17.529939431017137
36	16.082053501150824	33.051067697744486	33.48777118328987	17.379107617814814
37	16.90820895615184	31.450982147631144	33.44661262585379	18.19419627036323
38	15.760370153455517	33.243182414477864	33.36529027974141	17.631157152325212
39	19.831088264588594	29.658035804214588	31.12171368814248	19.38916224305434
40	14.70616396027391	27.733406946221557	34.35197654335265	23.20845255015189
41	16.537781939227088	28.124226722721886	32.172313844569864	23.16567749348116
42	16.566008502785976	29.16910695878051	32.72092881559101	21.543955722842504
43	16.467402049472337	29.864450321869867	33.39413857377516	20.274009054882637
44	14.1393944593867	36.878813539300204	29.73065392367885	19.25113807763424
45	17.886812723589824	32.407701002353875	31.394902058798298	18.31058421525801
46	16.433331219449716	31.55244856112916	31.1253197248967	20.88890049452442
47	14.912454131834215	29.517400370800058	35.32423865994701	20.245906837418726
48	16.876376355838733	27.631940532723544	35.68484233536888	19.80684077606885
49	17.2407104141098	31.474483559581053	32.08241161790434	19.202394408404803
50	14.730784487078575	31.651055014442797	31.539267875062016	22.078892623416607
51	14.857368811761152	28.634170058206408	35.19230745318059	21.316153676851858
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	756.0
1	4574.0
2	8392.0
3	30800.5
4	53209.0
5	31644.0
6	10079.0
7	9958.5
8	9838.0
9	11561.0
10	13284.0
11	14537.0
12	15790.0
13	15652.5
14	15515.0
15	13792.0
16	12069.0
17	10482.0
18	8895.0
19	7931.0
20	6967.0
21	6586.0
22	6205.0
23	5629.0
24	5053.0
25	5034.5
26	5841.0
27	6666.0
28	6971.0
29	7276.0
30	8921.5
31	10567.0
32	12981.0
33	15395.0
34	18791.0
35	22187.0
36	24002.5
37	25818.0
38	27511.0
39	29204.0
40	35388.5
41	41573.0
42	49578.5
43	57584.0
44	61934.0
45	66284.0
46	62749.5
47	59215.0
48	56149.5
49	53084.0
50	53654.5
51	54225.0
52	51163.5
53	48102.0
54	44443.5
55	40785.0
56	35043.5
57	29302.0
58	25521.5
59	21741.0
60	17805.5
61	13870.0
62	12055.0
63	10240.0
64	8116.0
65	5992.0
66	4804.0
67	3616.0
68	3193.5
69	2771.0
70	2561.5
71	2352.0
72	2275.0
73	2198.0
74	1885.0
75	1196.5
76	821.0
77	588.5
78	356.0
79	261.0
80	166.0
81	126.0
82	86.0
83	72.0
84	58.0
85	34.5
86	11.0
87	11.0
88	11.0
89	8.0
90	5.0
91	5.0
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	804207.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.58834402074905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.50966998726075	25.91088478521079
2	8.947001488913285	5.8313592494972015
3	3.164715717567616	3.0939853359589544
4	1.7170807823254919	2.2382727698336016
5	1.0508275740660376	1.7122365245076592
6	0.7060598459593886	1.3805592695616982
7	0.5060073416190457	1.1542958927994305
8	0.37166834458436127	0.9689644699949974
9	0.32069661907210506	0.9405874573751585
>10	2.6915978265057734	19.616848185820118
>50	0.7279962215793611	16.898576074376205
>100	0.2827131295157706	13.26383872085535
>500	0.0019825605155383633	0.4016055913843273
>1k	0.0015860484124306908	1.1297418550428486
>5k	0.0	0.0
>10k+	3.965121031076727E-4	5.45824381778165
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42241	5.252503397757045	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4608	0.5729868056358625	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1804	0.22432035533140102	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	1169	0.14536058502350763	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1162	0.1444901623586962	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	822	0.10221249006785565	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05757224197252697	0.0
2	0.0	0.0	0.0	0.2136265911637178	0.0
3	0.0	0.0	0.0	0.3461795284050002	0.0
4	0.0	0.0	0.0	0.4379469464951188	0.0
5	0.0	0.0	0.0	0.9691534642200329	0.0
6	0.0	0.0	0.0	1.1271973509307927	0.0
7	0.0	0.0	0.0	1.5590513387722316	0.0
8	0.0	0.0	0.0	2.055813988189608	0.0
9	0.0	0.0	0.0	2.414055087806995	0.0
10	0.0	0.0	0.0	2.6826426529488057	0.0
11	0.0	0.0	0.0	2.9785863589846895	0.0
12	0.0	0.0	0.0	3.224915973126322	0.0
13	0.0	0.0	0.0	3.336330074222184	0.0
14	0.0	0.0	0.0	3.4360556423905786	0.0
15	0.0	0.0	0.0	3.756619875231128	0.0
16	0.0	0.0	0.0	4.15813341589914	0.0
17	0.0	0.0	0.0	4.610504509411134	0.0
18	0.0	0.0	0.0	4.865040965820989	0.0
19	1.2434609497306043E-4	0.0	0.0	5.141337988851129	0.0
20	1.2434609497306043E-4	0.0	0.0	5.455187532563134	0.0
21	8.70422664811423E-4	0.0	0.0	5.791792411655208	0.0
22	8.70422664811423E-4	0.0	0.0	6.132127673596474	0.0
23	8.70422664811423E-4	0.0	0.0	6.403202160637746	0.0
24	0.0018651914245959064	0.0	0.0	6.658982077997331	0.0
25	0.0018651914245959064	0.0	0.0	6.897975272535554	0.0
26	0.0018651914245959064	0.0	0.0	7.157982957124223	0.0
27	0.0018651914245959064	0.0	0.0	7.412395067439105	0.0
28	0.0018651914245959064	0.0	0.0	7.681231324770861	0.0
29	0.0018651914245959064	0.0	0.0	7.994459138008001	0.0
30	0.0018651914245959064	0.0	0.0	8.299728801166864	0.0
31	0.0018651914245959064	0.0	0.0	8.582491821135603	0.0
32	0.0018651914245959064	0.0	0.0	8.872093876327861	0.0
33	0.0018651914245959064	0.0	0.0	9.171021888643098	0.0
34	0.0018651914245959064	0.0	0.0	9.54406017356228	0.0
35	0.0018651914245959064	0.0	0.0	9.83950649521827	0.0
36	0.0018651914245959064	0.0	0.0	10.138061469248589	0.0
37	0.0018651914245959064	0.0	0.0	10.444325901167236	0.0
38	0.0018651914245959064	0.0	0.0	10.756807637834537	0.0
39	0.0018651914245959064	0.0	0.0	11.101619359194833	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTTACT	30	2.1646847E-6	45.000004	8
TTTAGCG	25	3.8897575E-5	45.0	23
AACCGTA	20	7.0320227E-4	45.0	36
AACCGGT	25	3.8897575E-5	45.0	35
CGAACCT	20	7.0320227E-4	45.0	23
CTCGTCG	20	7.0320227E-4	45.0	39
TTTACGG	50	2.1827873E-11	45.0	1
CGCGGAT	20	7.0320227E-4	45.0	22
CCCTAAG	35	1.2113196E-7	45.0	18
TTATGTC	20	7.0320227E-4	45.0	14
TAGGTCG	20	7.0320227E-4	45.0	1
CATTGCG	20	7.0320227E-4	45.0	12
CGCATAC	25	3.8897575E-5	45.0	23
CAACCCG	25	3.8897575E-5	45.0	22
GCACGCT	25	3.8897575E-5	45.0	23
ATTGTCG	25	3.8897575E-5	45.0	35
TACGGTA	20	7.0320227E-4	45.0	44
TTATCGG	20	7.0320227E-4	45.0	1
ATCCGGA	25	3.8897575E-5	45.0	10
CCGTATG	20	7.0320227E-4	45.0	34
>>END_MODULE