##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043394_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1136632
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50852958565305	31.0	31.0	33.0	30.0	34.0
2	30.51858825019883	31.0	31.0	34.0	26.0	34.0
3	31.442108791587778	31.0	31.0	34.0	28.0	34.0
4	35.054225993989256	37.0	35.0	37.0	32.0	37.0
5	35.366725554093144	37.0	35.0	37.0	33.0	37.0
6	35.813810450524	37.0	35.0	37.0	35.0	37.0
7	33.700002287459796	37.0	35.0	37.0	32.0	37.0
8	34.74198157363157	37.0	35.0	37.0	31.0	37.0
9	36.72992314135094	39.0	37.0	39.0	32.0	39.0
10	36.67799340507746	39.0	35.0	39.0	32.0	39.0
11	36.343510476565854	38.0	35.0	39.0	32.0	39.0
12	36.25858237318675	39.0	35.0	39.0	32.0	39.0
13	36.284210720796175	39.0	35.0	39.0	32.0	39.0
14	37.628641460032796	40.0	35.0	41.0	32.0	41.0
15	37.80829327346054	40.0	35.0	41.0	33.0	41.0
16	37.769782128252594	40.0	35.0	41.0	33.0	41.0
17	37.70868583675279	40.0	36.0	41.0	33.0	41.0
18	37.42241992131138	39.0	36.0	41.0	33.0	41.0
19	37.10622259447209	38.0	35.0	41.0	32.0	41.0
20	36.80381073205752	38.0	35.0	40.0	32.0	41.0
21	36.67452174494471	38.0	35.0	40.0	32.0	41.0
22	36.60310812998402	38.0	35.0	40.0	32.0	41.0
23	36.49920114865673	37.0	35.0	40.0	32.0	41.0
24	36.34006257082327	37.0	35.0	40.0	32.0	41.0
25	36.2319264282547	37.0	35.0	40.0	31.0	41.0
26	35.960080307434595	36.0	35.0	40.0	31.0	41.0
27	35.842788167146445	36.0	35.0	40.0	31.0	41.0
28	35.77499313762062	36.0	35.0	40.0	31.0	41.0
29	35.70169764708366	36.0	35.0	40.0	30.0	41.0
30	35.48479543071108	36.0	35.0	40.0	30.0	41.0
31	35.02009797366254	36.0	34.0	40.0	27.0	41.0
32	34.83451636061628	36.0	34.0	40.0	25.0	41.0
33	34.680432189134216	36.0	34.0	40.0	24.0	41.0
34	34.43376396230266	36.0	34.0	40.0	23.0	41.0
35	34.19979729587061	36.0	34.0	40.0	21.0	41.0
36	34.025050324115455	36.0	33.0	40.0	21.0	41.0
37	33.9406588939956	36.0	33.0	40.0	20.0	41.0
38	33.854940737195506	36.0	33.0	40.0	20.0	41.0
39	33.729047748083815	35.0	33.0	40.0	18.0	41.0
40	33.682299108242596	35.0	33.0	40.0	18.0	41.0
41	33.604584421343056	35.0	33.0	40.0	18.0	41.0
42	33.523412150986424	35.0	33.0	40.0	18.0	41.0
43	33.36278232532605	35.0	33.0	40.0	18.0	41.0
44	33.16204893052457	35.0	33.0	40.0	17.0	41.0
45	32.97727760612054	35.0	33.0	40.0	15.0	41.0
46	32.9998785886725	35.0	33.0	40.0	17.0	41.0
47	32.974827384764815	35.0	33.0	40.0	17.0	41.0
48	32.9033416268414	35.0	33.0	40.0	15.0	41.0
49	32.8144421413439	35.0	33.0	40.0	15.0	41.0
50	32.702678615418186	35.0	32.0	40.0	15.0	41.0
51	32.26687793410708	35.0	31.0	39.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	1.0
13	14.0
14	29.0
15	62.0
16	157.0
17	357.0
18	770.0
19	1406.0
20	2372.0
21	3778.0
22	6039.0
23	9288.0
24	14546.0
25	21703.0
26	27117.0
27	29594.0
28	28839.0
29	29093.0
30	31819.0
31	36475.0
32	44503.0
33	58269.0
34	97959.0
35	135603.0
36	118777.0
37	100987.0
38	131415.0
39	205548.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.375935219138647	18.606813814849485	25.28285320138796	30.734397764623907
2	32.71454613278528	19.68068820867264	29.421747760048987	18.18301789849309
3	20.62347356048396	21.452677735625954	40.10832001914428	17.81552868474581
4	21.904539024064075	24.48224227366465	36.78173762484252	16.83148107742875
5	19.153692663940483	30.641227767650392	34.431196728580574	15.773882839828545
6	77.16217738018989	2.1114133686188667	19.228299044897557	1.4981102062936817
7	73.67732036402283	7.43600391331583	17.37844790574258	1.5082278169187564
8	72.40003800702426	4.233208285531289	20.433086522286896	2.933667185157553
9	40.97729080300396	20.889082834197875	26.435468999641042	11.698157363157117
10	31.13743058439319	23.7680269427572	35.04749118448187	10.04705128836774
11	28.896247862104886	21.250413502347286	37.9188690798781	11.934469555669732
12	20.33349404204703	28.835366239908783	35.913558653988275	14.917581064055913
13	14.70871838906524	31.71774153815835	36.64352226578171	16.930017806994698
14	13.827078597118506	23.924717938611618	45.29108805664454	16.957115407625334
15	16.290056940153015	21.499394702946955	44.95808669824534	17.252461658654692
16	17.636578945516227	23.622245370533296	36.22597287424602	22.51520280970446
17	18.878581634161275	23.084252422947795	40.91746493148178	17.11970101140915
18	22.661160340374018	24.679315732796542	36.35477445646436	16.304749470365078
19	22.127214437038546	24.859321222700046	36.72604677679319	16.287417563468214
20	17.59223741721155	28.390367330851145	37.04593923099121	16.971456020946093
21	18.244867292140288	24.989970368597753	36.24031348756678	20.524848851695186
22	15.280407378993377	29.152707296644824	36.21691101429486	19.349974310066933
23	15.262635575982376	24.99674476875541	45.51807445153753	14.222545203724687
24	15.71124163317591	25.9887984853497	41.396071903659234	16.90388797781516
25	15.727077893284722	29.8946360827427	37.65290788927287	16.72537813469971
26	15.136385391226009	30.61826519049261	37.08192273312734	17.163426685154036
27	15.42812449411947	27.852198424819996	41.17638778426087	15.543289296799667
28	14.64528536940716	25.433209693198854	41.16099142026619	18.760513517127794
29	16.140140344456253	30.09118166653763	38.462316739278855	15.306361249727265
30	20.6686948810169	27.620373172671542	36.33709063267619	15.373841313635372
31	20.129910120425958	27.43948789053977	36.220958058544895	16.209643930489374
32	19.024715123276486	28.536060923852226	36.69191083833642	15.747313114534872
33	16.98773217716904	27.556852173790634	36.04271215309792	19.4127034959424
34	16.43900576439868	27.612279084171483	38.26568317626109	17.683031975168745
35	21.46710632816954	28.196285165295365	34.373042462292105	15.963566044242992
36	16.845469773858206	30.975636793614818	35.84220750427579	16.336685928251185
37	16.908023001288015	31.402951878884284	35.00746063809571	16.681564481731996
38	17.266626313529798	31.388347327895044	34.65703939357681	16.68798696499835
39	20.13228555944228	28.40602763251431	33.91537454514742	17.546312262895995
40	15.708074381154146	27.73888118581916	36.096555437467885	20.456488995558807
41	17.277183820269006	27.941409356766307	33.7738159756192	21.00759084734549
42	17.350822429774983	27.541015913681825	35.45026006658268	19.657901589960513
43	17.70221144574497	29.064200198481128	34.765869692213485	18.467718663560415
44	15.089844382350664	33.6678889913358	33.154530226141794	18.087736400171735
45	17.404665714144947	30.853961528445446	34.381136550792164	17.360236206617447
46	17.288357181568	29.922261558710296	33.69859374010234	19.09078751961937
47	16.164510589179258	28.892112838632027	36.667452614390584	18.275923957798128
48	17.46581127400953	26.998096129618034	37.13022332645922	18.405869269913218
49	17.554670289064532	30.15215126795656	34.191101429486416	18.102077013492494
50	15.98406520316162	30.071826237515747	34.32139865849281	19.622709900829822
51	16.078378930031885	27.12399439748309	36.981362481436385	19.816264191048642
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1110.0
1	6807.5
2	12505.0
3	46311.0
4	80117.0
5	48369.0
6	16621.0
7	16496.0
8	16371.0
9	18475.0
10	20579.0
11	22358.0
12	24137.0
13	23161.5
14	22186.0
15	19877.5
16	17569.0
17	15324.5
18	13080.0
19	11456.5
20	9833.0
21	9060.0
22	8287.0
23	7597.0
24	6907.0
25	7015.5
26	7532.0
27	7940.0
28	8975.5
29	10011.0
30	12960.5
31	15910.0
32	18533.5
33	21157.0
34	25278.0
35	29399.0
36	32719.0
37	36039.0
38	40180.5
39	44322.0
40	51023.0
41	57724.0
42	68411.0
43	79098.0
44	87011.0
45	94924.0
46	95576.5
47	96229.0
48	89790.5
49	83352.0
50	76844.0
51	70336.0
52	64389.5
53	58443.0
54	52453.0
55	46463.0
56	42825.0
57	39187.0
58	35289.5
59	31392.0
60	26157.0
61	20922.0
62	16685.0
63	12448.0
64	10457.0
65	8466.0
66	6729.5
67	4993.0
68	4392.5
69	3792.0
70	3205.0
71	2618.0
72	2403.5
73	2189.0
74	1778.5
75	1071.5
76	775.0
77	603.5
78	432.0
79	312.5
80	193.0
81	115.5
82	38.0
83	25.5
84	13.0
85	14.5
86	16.0
87	10.5
88	5.0
89	6.5
90	8.0
91	6.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1136632.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.15684947730441
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.38625276863898	27.11584286385642
2	9.240113120943638	6.312263060506747
3	3.255177192916915	3.335597922012522
4	1.6938781995064993	2.3143017077372363
5	1.0002221218241503	1.708221822950877
6	0.7294403109285799	1.494922974183938
7	0.5036911938860558	1.2043153004826832
8	0.4006716404843875	1.0948544731079883
9	0.32275133296508296	0.9921751828818928
>10	2.589910113899681	19.433098276320003
>50	0.6249766579079148	15.148986004909041
>100	0.24733046463888192	12.167070030004828
>500	0.003989201042356431	0.9136589271186509
>1k	0.0010637869446283818	0.5327708895954353
>5k	2.6594673615709544E-4	0.6067138570516474
>10k+	2.6594673615709544E-4	5.625206707280022
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	61925	5.448113373545704	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6679	0.587613229259778	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2587	0.22760224945276922	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1211	0.1065428388431788	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08833113971804418	0.0
2	0.0	0.0	0.0	0.2934107081271687	0.0
3	0.0	0.0	0.0	0.47684738772091584	0.0
4	0.0	0.0	0.0	0.5978188191076795	0.0
5	0.0	0.0	0.0	1.3114182954553453	0.0
6	0.0	0.0	0.0	1.5069081285763555	0.0
7	0.0	0.0	0.0	2.073230385912063	0.0
8	0.0	0.0	0.0	2.7248924893897057	0.0
9	0.0	0.0	0.0	3.1970769783008044	0.0
10	0.0	0.0	0.0	3.5606951062437093	0.0
11	0.0	0.0	0.0	3.9604726947684035	0.0
12	0.0	0.0	0.0	4.2774618346131374	0.0
13	0.0	0.0	0.0	4.431249516114274	0.0
14	0.0	0.0	0.0	4.551605092941251	0.0
15	0.0	0.0	0.0	4.970914068933481	0.0
16	0.0	0.0	0.0	5.467292844121932	0.0
17	0.0	0.0	0.0	6.017514903680347	0.0
18	0.0	0.0	0.0	6.3382871501066305	0.0
19	0.0	0.0	0.0	6.6736639475221535	0.0
20	0.0	0.0	0.0	7.08065583231864	0.0
21	0.0	0.0	0.0	7.495829784838013	0.0
22	0.0	0.0	0.0	7.905373067096474	0.0
23	8.797922282673724E-5	0.0	0.0	8.245676700990295	0.0
24	0.0020235221250149565	0.0	0.0	8.563281695394815	0.0
25	0.0020235221250149565	0.0	0.0	8.868745557049246	0.0
26	0.0020235221250149565	0.0	0.0	9.227700786182336	0.0
27	0.0020235221250149565	0.0	0.0	9.560702144581535	0.0
28	0.0020235221250149565	0.0	0.0	9.894847232877483	0.0
29	0.0020235221250149565	0.0	0.0	10.285562961451024	0.0
30	0.0020235221250149565	0.0	0.0	10.660970305252711	0.0
31	0.0020235221250149565	0.0	0.0	11.012183362777046	0.0
32	0.0020235221250149565	0.0	0.0	11.3618127942905	0.0
33	0.0020235221250149565	0.0	0.0	11.724287192336657	0.0
34	0.0020235221250149565	0.0	0.0	12.155649321856151	0.0
35	0.0020235221250149565	0.0	0.0	12.511701236635956	0.0
36	0.0020235221250149565	0.0	0.0	12.86511377473096	0.0
37	0.0020235221250149565	0.0	0.0	13.257501108538207	0.0
38	0.0020235221250149565	0.0	0.0	13.656486884057461	0.0
39	0.0020235221250149565	0.0	0.0	14.058463953152824	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCAG	95	0.0	45.000004	34
GTACCGG	20	7.0332544E-4	45.0	1
CGACGTG	20	7.0332544E-4	45.0	24
CGAGTAC	20	7.0332544E-4	45.0	16
TAGCGTA	20	7.0332544E-4	45.0	38
CCTACGG	20	7.0332544E-4	45.0	11
TTCGTGG	70	0.0	45.0	1
TCGATCT	20	7.0332544E-4	45.0	10
GCGCAGA	20	7.0332544E-4	45.0	1
CCGTCGC	40	6.8157533E-9	45.0	29
CGTTAGG	20	7.0332544E-4	45.0	38
ACCTATC	50	2.1827873E-11	45.0	20
CTAACGG	55	1.8189894E-12	45.0	1
CGCTAGC	35	1.2119199E-7	45.0	36
ACAATCG	20	7.0332544E-4	45.0	9
CGTGTAT	20	7.0332544E-4	45.0	21
ACGTAGG	70	0.0	45.0	1
CCGCGAA	25	3.890779E-5	45.0	18
CGACTTT	20	7.0332544E-4	45.0	15
GACGATT	35	1.2119199E-7	45.0	8
>>END_MODULE