FastQCFastQC Report
Thu 26 May 2016
SRR1043393_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043393_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1225225
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT503364.1083066375563675No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54950.4484890530310759No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22270.1817625334122304No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGTC351.2120108E-745.00000437
TATCACG207.03347E-445.033
ATCTACG207.03347E-445.011
ACGTTAC253.890958E-545.023
CGCATCG207.03347E-445.020
GCCGGAT253.890958E-545.017
CGACATC207.03347E-445.010
CGTTACA253.890958E-545.024
ACGATAA253.890958E-545.017
CGTAGTC207.03347E-445.030
TTGACGT207.03347E-445.017
TTGACGG1000.045.01
CACAGCG253.890958E-545.045
GCGATCG453.8562575E-1045.08
GCGATAT800.045.08
AACTCGC207.03347E-445.012
GCGTCTC207.03347E-445.08
ATACCCG207.03347E-445.030
GCGAATA302.1656197E-644.99999616
TCTTCGC302.1656197E-644.9999961