FastQCFastQC Report
Thu 26 May 2016
SRR1043392_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043392_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3264783
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1217123.728027253266144No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC328931.0075095343243334No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124050.37996399760719163No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC116840.35787983458624967No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC83640.2561885430057679No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC54500.1669329937089234No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC53530.16396189271997558No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51040.15633504585143945No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA50100.1534558345837993No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT372000.044.1411251
CGTTTTA19600.042.5892871
CGCACTT100400.041.43675634
CCCGCAC101500.041.25369632
ACCCGCA100550.041.218331
CCGCACT101300.041.0908233
GCACTTA101700.040.8849635
CTTACTG101000.040.63366738
TAGGGCG11250.040.64
ACTACGG2400.040.3124961
CTAGCGG8750.040.1142851
CAACGCA107600.040.04414716
TCAACGC107950.039.9768415
CGCAAGC107800.039.90723819
ATCAACG108100.039.90055514
AACGCAA107800.039.86549417
ACGCAAG107900.039.849418
TGACCCG104200.039.73128529
CACTTAC104750.039.69450836
GCTTATG107750.039.6125324