##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043392_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3264783
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53961748759412	31.0	31.0	33.0	30.0	34.0
2	30.582898771526317	31.0	31.0	34.0	26.0	34.0
3	31.547438221774616	31.0	31.0	34.0	28.0	34.0
4	35.155341717964106	37.0	35.0	37.0	32.0	37.0
5	35.475465291261315	37.0	35.0	37.0	33.0	37.0
6	35.79676107110335	37.0	35.0	37.0	35.0	37.0
7	33.75177645803718	37.0	35.0	37.0	32.0	37.0
8	34.77755703824726	37.0	35.0	37.0	32.0	37.0
9	36.72626542100961	39.0	37.0	39.0	32.0	39.0
10	36.80696879394434	39.0	35.0	39.0	32.0	39.0
11	36.67503475728709	39.0	35.0	39.0	33.0	39.0
12	36.66127733451197	39.0	35.0	39.0	33.0	39.0
13	36.68317159210888	39.0	35.0	39.0	33.0	39.0
14	37.951796489996426	40.0	36.0	41.0	33.0	41.0
15	38.02614538240367	40.0	36.0	41.0	33.0	41.0
16	37.88772668811373	40.0	36.0	41.0	33.0	41.0
17	37.84575422011203	40.0	36.0	41.0	33.0	41.0
18	37.654243482644944	39.0	36.0	41.0	33.0	41.0
19	37.43704650508165	39.0	36.0	41.0	33.0	41.0
20	37.18651316182423	39.0	35.0	41.0	33.0	41.0
21	37.079563327792386	39.0	35.0	41.0	32.0	41.0
22	37.004547928606584	38.0	35.0	41.0	32.0	41.0
23	36.93454266332556	38.0	35.0	40.0	32.0	41.0
24	36.79338871833136	38.0	35.0	40.0	32.0	41.0
25	36.721786409694	38.0	35.0	40.0	32.0	41.0
26	36.46044193442566	38.0	35.0	40.0	31.0	41.0
27	36.35117525422057	38.0	35.0	40.0	31.0	41.0
28	36.183118755519125	38.0	35.0	40.0	31.0	41.0
29	36.106664363297654	38.0	35.0	40.0	30.0	41.0
30	35.91396518543499	38.0	35.0	40.0	30.0	41.0
31	35.64392886142816	38.0	35.0	40.0	29.0	41.0
32	35.50975669745891	38.0	35.0	40.0	27.0	41.0
33	35.23444192156109	38.0	35.0	40.0	25.0	41.0
34	35.04131484389621	38.0	34.0	40.0	24.0	41.0
35	34.874233907735984	38.0	34.0	40.0	23.0	41.0
36	34.74134574947248	38.0	34.0	40.0	23.0	41.0
37	34.61836636615665	38.0	34.0	40.0	22.0	41.0
38	34.49466534222948	37.0	34.0	40.0	22.0	41.0
39	34.383824591098396	37.0	34.0	40.0	21.0	41.0
40	34.27710876955681	37.0	34.0	40.0	21.0	41.0
41	34.18430811481192	37.0	34.0	40.0	21.0	41.0
42	34.08297059865847	37.0	33.0	40.0	20.0	41.0
43	33.91880440445812	37.0	33.0	40.0	20.0	41.0
44	33.701688289849585	36.0	33.0	40.0	18.0	41.0
45	33.496734392454265	36.0	33.0	40.0	18.0	41.0
46	33.49639807607428	36.0	33.0	40.0	19.0	41.0
47	33.45400383425177	36.0	33.0	40.0	19.0	41.0
48	33.372423527076684	35.0	33.0	40.0	18.0	41.0
49	33.319208964271134	35.0	33.0	40.0	18.0	41.0
50	33.243876239247754	35.0	33.0	40.0	18.0	41.0
51	32.819221369383506	35.0	32.0	39.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	7.0
11	8.0
12	15.0
13	27.0
14	90.0
15	156.0
16	368.0
17	909.0
18	1832.0
19	3393.0
20	5716.0
21	9017.0
22	13939.0
23	22187.0
24	34629.0
25	53120.0
26	69458.0
27	78752.0
28	81337.0
29	81814.0
30	87014.0
31	99287.0
32	118396.0
33	153109.0
34	263142.0
35	297598.0
36	307923.0
37	339328.0
38	466483.0
39	675393.0
40	330.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.880811680286254	17.599270763171702	24.261551227141283	30.25836632940076
2	32.69133048046379	20.06212970356682	27.60958385289313	19.63695596307626
3	24.88447164788594	20.956002282540677	35.547079239263375	18.61244683031001
4	22.059475315817316	24.25012627179203	32.68005867465005	21.010339737740612
5	19.82796406376779	33.21127315352965	31.02350140882258	15.937261373879979
6	80.44991045346659	2.006657104009669	16.224631162316147	1.3188012802075972
7	76.43390081362223	7.1613029104844035	15.041734779922585	1.363061495970789
8	76.08671081661477	3.254060070761211	17.7754539888256	2.883775123798427
9	35.500919969259826	20.28269566461232	25.28171091309897	18.934673453028882
10	28.99175841089592	23.467501515414654	30.77659372766888	16.764146346020546
11	27.447429124692206	19.05753001041723	36.170122179636444	17.32491868525412
12	23.316924892098495	19.99388627054233	36.60699654464018	20.082192292718997
13	19.928368899249964	24.081937451891903	36.47112227673325	19.518571372124885
14	18.907167796450793	22.45748032870791	38.73228327885804	19.903068595983257
15	22.137765358371446	18.640810124286975	39.77250555396791	19.448918963373675
16	21.80056071107942	19.55315253724367	36.505703441852035	22.14058330982488
17	20.79023322530165	22.778267345792965	36.98885959648773	19.44263983241765
18	23.31147276863424	25.428703837284132	33.82123712356993	17.4385862705117
19	23.427590746460027	23.104322706899662	33.68266742383797	19.78541912280234
20	23.61035940214097	24.504231981114824	35.6493218691717	16.236086747572504
21	20.275803935514244	21.57984160049841	36.0425486165543	22.101805847433045
22	20.399885689186693	24.17339835450013	35.81196667588627	19.614749280426906
23	21.45422835147083	23.514885981702307	37.74949207956547	17.28139358726139
24	24.220721560973576	21.420474193843816	35.313587457420596	19.045216787762005
25	19.33819184919794	23.408140755449903	35.127541401679686	22.126125993672474
26	17.909796761377404	22.564868783009466	38.27448868730326	21.25084576830987
27	19.786154240572802	22.998802676931362	38.06954397888006	19.14549910361577
28	20.146668247169874	25.048433540605913	35.60781834504774	19.19707986717647
29	20.76373835565794	22.094975378149176	38.889751631272276	18.251534634920606
30	24.439939806106562	22.44758686871379	35.38728301390935	17.725190311270307
31	23.485971349397495	23.851692440202	33.90274330636983	18.75959290403068
32	23.848139370978103	21.948380642756348	33.1099187909273	21.09356119533825
33	20.431097564524197	23.643623481254345	34.9226885829778	21.002590371243663
34	20.309159904348924	23.720841477059885	34.31048862971903	21.659509988872156
35	23.168339212744	26.055024177717172	31.7682982299283	19.008338379610528
36	20.7177016052828	25.966748785447606	31.918874853244457	21.39667475602513
37	22.33854439942869	28.038034993443667	30.7731631780734	18.850257429054242
38	20.770844494105734	26.014776479784413	30.297266311421005	22.917112714688848
39	21.36233863016317	23.660745599324674	34.63580887305527	20.34110689745689
40	19.642040527655283	23.9512396382853	34.79937257698291	21.607347257076505
41	19.629482265743235	28.423543004236425	30.08153987569771	21.86543485432263
42	19.03762057080057	23.804154824378834	31.966810657859956	25.19141394696064
43	20.540048144087983	23.506370867527796	34.53375002258955	21.419830965794663
44	22.10652285312684	25.30900216032735	32.16964190269307	20.41483308385274
45	23.399135562761753	24.735120220853883	32.30343333691703	19.562310879467333
46	21.578095695793564	26.300737292493864	31.047484626083875	21.073682385628693
47	19.246179608261865	27.962042193922233	32.61337736688779	20.17840083092812
48	21.0318419325266	26.387328039872788	32.34986827608451	20.230961751516105
49	20.30125738831647	23.548640139329322	34.40703409690629	21.743068375447923
50	18.712637256442466	24.964017516631273	35.83080406875434	20.49254115817192
51	18.321340193207327	22.711800447380423	34.69648671902543	24.27037264038682
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1304.0
1	13718.0
2	26132.0
3	104186.5
4	182241.0
5	112089.0
6	41937.0
7	38375.0
8	34813.0
9	35294.0
10	35775.0
11	36128.0
12	36481.0
13	35478.5
14	34476.0
15	31974.5
16	29473.0
17	27202.5
18	24932.0
19	23516.0
20	22100.0
21	21721.0
22	21342.0
23	19984.5
24	18627.0
25	19876.5
26	22702.5
27	24279.0
28	27722.0
29	31165.0
30	34907.0
31	38649.0
32	44494.5
33	50340.0
34	57822.0
35	65304.0
36	72537.0
37	79770.0
38	84896.0
39	90022.0
40	98731.0
41	107440.0
42	122304.5
43	137169.0
44	148917.5
45	160666.0
46	179971.0
47	199276.0
48	224526.0
49	249776.0
50	245148.0
51	240520.0
52	216499.5
53	192479.0
54	182516.0
55	172553.0
56	172779.5
57	173006.0
58	167327.0
59	161648.0
60	152171.5
61	142695.0
62	133270.0
63	123845.0
64	109469.5
65	95094.0
66	78138.5
67	61183.0
68	48847.5
69	36512.0
70	32656.5
71	28801.0
72	30838.0
73	32875.0
74	27248.5
75	15343.5
76	9065.0
77	6708.0
78	4351.0
79	3316.0
80	2281.0
81	1575.0
82	869.0
83	671.5
84	474.0
85	304.5
86	135.0
87	115.0
88	95.0
89	67.0
90	39.0
91	28.0
92	17.0
93	11.5
94	6.0
95	4.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3264783.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.128392663047094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.88969814029744	21.130223155561982
2	8.846277509133985	4.799705797481379
3	2.976475507252776	2.422409889380868
4	1.5520791038160437	1.6842164548972753
5	0.9583596906178086	1.2999378999758124
6	0.6705132449599139	1.0913967957027857
7	0.5271860213047661	1.0011196574697645
8	0.42128044749647225	0.9142929120758796
9	0.35519903173566414	0.8672380925813303
>10	4.5859938793171215	29.373011775794765
>50	1.0065983072242244	18.691992198856088
>100	0.20167569420953255	7.973048371729067
>500	0.005414639146456063	1.0134135832719697
>1k	0.0021658556585824186	0.9492061324475146
>5k	6.016265718284496E-4	0.955799881845857
>10k+	4.813012574627596E-4	5.832987400927685
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	121712	3.728027253266144	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	32893	1.0075095343243334	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12405	0.37996399760719163	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	11684	0.35787983458624967	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8364	0.2561885430057679	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5450	0.1669329937089234	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5353	0.16396189271997558	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5104	0.15633504585143945	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	5010	0.1534558345837993	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04242242133703833	0.0
2	0.0	0.0	0.0	0.135384189393292	0.0
3	0.0	0.0	0.0	0.22326139287052157	0.0
4	0.0	0.0	0.0	0.28121317710855515	0.0
5	0.0	0.0	0.0	0.6154773533187351	0.0
6	0.0	0.0	0.0	0.7419176098380811	0.0
7	0.0	0.0	0.0	1.074466511250518	0.0
8	0.0	0.0	0.0	1.3954373077781892	0.0
9	0.0	0.0	0.0	1.726761012906524	0.0
10	0.0	0.0	0.0	1.9347380821328708	0.0
11	3.0629907102554747E-5	0.0	0.0	2.1252254744036585	0.0
12	3.0629907102554747E-5	0.0	0.0	2.2974880719484267	0.0
13	3.0629907102554747E-5	0.0	0.0	2.379269923912248	0.0
14	3.0629907102554747E-5	0.0	0.0	2.443776508270228	0.0
15	3.0629907102554747E-5	0.0	0.0	2.6326405154645807	0.0
16	3.0629907102554747E-5	0.0	0.0	2.8366050668604927	0.0
17	3.0629907102554747E-5	0.0	0.0	3.1091193503519223	0.0
18	3.0629907102554747E-5	0.0	0.0	3.2528042445700067	0.0
19	3.0629907102554747E-5	0.0	0.0	3.425097472021877	0.0
20	6.125981420510949E-5	0.0	0.0	3.629613361745635	0.0
21	6.125981420510949E-5	0.0	0.0	3.8359670458955466	0.0
22	6.125981420510949E-5	0.0	0.0	4.059871666815222	0.0
23	6.125981420510949E-5	0.0	0.0	4.250757247878343	0.0
24	9.188972130766425E-5	0.0	0.0	4.425500867898418	0.0
25	9.188972130766425E-5	0.0	0.0	4.5967526785088015	0.0
26	9.188972130766425E-5	0.0	0.0	4.7812059790803865	0.0
27	9.188972130766425E-5	0.0	0.0	4.983485885585657	0.0
28	9.188972130766425E-5	0.0	0.0	5.186225240697468	0.0
29	9.188972130766425E-5	0.0	0.0	5.390955539770943	0.0
30	9.188972130766425E-5	0.0	0.0	5.598013711784214	0.0
31	9.188972130766425E-5	0.0	0.0	5.7974144070218445	0.0
32	9.188972130766425E-5	0.0	0.0	6.027873828061467	0.0
33	9.188972130766425E-5	0.0	0.0	6.260936791204806	0.0
34	9.188972130766425E-5	0.0	0.0	6.5281827306745965	0.0
35	9.188972130766425E-5	0.0	0.0	6.75591608998209	0.0
36	9.188972130766425E-5	0.0	0.0	7.006989438501732	0.0
37	9.188972130766425E-5	0.0	0.0	7.291388125948953	0.0
38	9.188972130766425E-5	0.0	0.0	7.592939561373604	0.0
39	9.188972130766425E-5	0.0	0.0	7.929286571266758	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	37200	0.0	44.141125	1
CGTTTTA	1960	0.0	42.589287	1
CGCACTT	10040	0.0	41.436756	34
CCCGCAC	10150	0.0	41.253696	32
ACCCGCA	10055	0.0	41.2183	31
CCGCACT	10130	0.0	41.09082	33
GCACTTA	10170	0.0	40.88496	35
CTTACTG	10100	0.0	40.633667	38
TAGGGCG	1125	0.0	40.6	4
ACTACGG	240	0.0	40.312496	1
CTAGCGG	875	0.0	40.114285	1
CAACGCA	10760	0.0	40.044147	16
TCAACGC	10795	0.0	39.97684	15
CGCAAGC	10780	0.0	39.907238	19
ATCAACG	10810	0.0	39.900555	14
AACGCAA	10780	0.0	39.865494	17
ACGCAAG	10790	0.0	39.8494	18
TGACCCG	10420	0.0	39.731285	29
CACTTAC	10475	0.0	39.694508	36
GCTTATG	10775	0.0	39.61253	24
>>END_MODULE