FastQCFastQC Report
Thu 26 May 2016
SRR1043391_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043391_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3599051
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060982.9479437774013206No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC252840.7025185250222906No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107860.2996901127547234No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC87030.241813744789946No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC74800.20783256475109688No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC52120.14481595287202098No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC47030.1306733358321402No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43990.12222666475134696No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA41270.11466911694221617No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA302.1667965E-644.9999961
CGTTTTT328750.043.7749021
TATACGG2100.040.7142871
CACTTAC80250.040.54205336
TTATCGG1450.040.344831
CGCACTT80550.040.3072634
ACCCGCA80700.040.1208231
CCGCACT80950.040.080333
GCACTTA81350.039.93853835
ACTTACT81150.039.92606437
CTTACTG81150.039.92606438
CCCGCAC82150.039.6317732
ACTACGG1950.039.230771
TATGACC83750.039.22387727
AACGCAA86150.039.12362317
CAACGCA86000.039.11337316
TCAACGC86300.039.0816915
CGCAAGC86600.038.99826419
ATCAACG86600.038.97228214
TTATGAC84700.038.91676726