##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043391_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3599051
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.577411656572803	31.0	31.0	33.0	30.0	34.0
2	30.647267293517096	31.0	31.0	34.0	26.0	34.0
3	31.612341142151084	31.0	31.0	34.0	28.0	34.0
4	35.23629340067701	37.0	35.0	37.0	33.0	37.0
5	35.56795055140925	37.0	35.0	37.0	33.0	37.0
6	35.800619107648096	37.0	35.0	37.0	35.0	37.0
7	33.82604080909106	37.0	35.0	37.0	32.0	37.0
8	34.806039703244	37.0	35.0	37.0	32.0	37.0
9	36.84441037373463	39.0	37.0	39.0	32.0	39.0
10	36.82289053419915	39.0	35.0	39.0	32.0	39.0
11	36.692729555652306	39.0	35.0	39.0	33.0	39.0
12	36.719788355319224	39.0	35.0	39.0	33.0	39.0
13	36.75051006501436	39.0	35.0	39.0	33.0	39.0
14	37.931977901952486	40.0	36.0	41.0	33.0	41.0
15	38.03589474003008	40.0	36.0	41.0	33.0	41.0
16	37.91471168371885	40.0	36.0	41.0	33.0	41.0
17	37.80330203712034	40.0	36.0	41.0	33.0	41.0
18	37.64538318573424	39.0	36.0	41.0	33.0	41.0
19	37.47683764414564	39.0	36.0	41.0	33.0	41.0
20	37.181252780246794	39.0	35.0	41.0	32.0	41.0
21	37.10862057803571	39.0	35.0	41.0	32.0	41.0
22	37.07473164453629	39.0	35.0	41.0	32.0	41.0
23	37.015322372480966	38.0	35.0	41.0	32.0	41.0
24	36.906100524832794	38.0	35.0	40.0	32.0	41.0
25	36.81176676851759	38.0	35.0	40.0	32.0	41.0
26	36.540452191424905	38.0	35.0	40.0	31.0	41.0
27	36.41747255040287	38.0	35.0	40.0	31.0	41.0
28	36.26480313838287	38.0	35.0	40.0	31.0	41.0
29	36.19487359306662	38.0	35.0	40.0	31.0	41.0
30	36.042607898582155	38.0	35.0	40.0	30.0	41.0
31	35.834371060593476	38.0	35.0	40.0	30.0	41.0
32	35.70823864402033	38.0	35.0	40.0	29.0	41.0
33	35.37951948999889	38.0	34.0	40.0	27.0	41.0
34	35.26543302665063	38.0	34.0	40.0	26.0	41.0
35	35.138711565910015	38.0	34.0	40.0	25.0	41.0
36	35.0167880366241	38.0	34.0	40.0	25.0	41.0
37	34.88327172913082	37.0	34.0	40.0	24.0	41.0
38	34.76872014317107	37.0	34.0	40.0	24.0	41.0
39	34.63887007991829	37.0	34.0	40.0	23.0	41.0
40	34.531023039128925	37.0	34.0	40.0	23.0	41.0
41	34.41194470431233	36.0	34.0	40.0	23.0	41.0
42	34.29964232237887	36.0	34.0	40.0	23.0	41.0
43	34.12964973266564	36.0	33.0	40.0	23.0	41.0
44	33.90515055218723	36.0	33.0	40.0	22.0	41.0
45	33.674087419155775	35.0	33.0	40.0	21.0	41.0
46	33.65519910665339	35.0	33.0	40.0	22.0	41.0
47	33.61386459930687	35.0	33.0	40.0	22.0	41.0
48	33.504847805713226	35.0	33.0	39.0	22.0	41.0
49	33.437814301603396	35.0	33.0	39.0	21.0	41.0
50	33.37183329716639	35.0	33.0	39.0	21.0	41.0
51	32.920026973777254	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	4.0
11	6.0
12	29.0
13	26.0
14	74.0
15	187.0
16	398.0
17	800.0
18	1584.0
19	2956.0
20	5073.0
21	8361.0
22	13149.0
23	20293.0
24	31937.0
25	49010.0
26	65134.0
27	76298.0
28	81607.0
29	85012.0
30	93383.0
31	109002.0
32	133171.0
33	177353.0
34	305047.0
35	352488.0
36	370454.0
37	422172.0
38	527428.0
39	666273.0
40	336.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.6438144388618	17.611225848147193	24.258589278118038	28.48637043487297
2	31.16993896446591	20.92960060860488	26.681366838091485	21.219093588837723
3	25.80785879388761	22.041004698182938	32.50873633077164	19.64240017715781
4	23.94706271180931	24.390818579675585	29.687992751422527	21.97412595709258
5	22.04995150110404	33.55356731538397	27.819527981126136	16.57695320238585
6	82.62525315701278	2.2609849096331227	13.604058403173502	1.5097035301805948
7	78.47115809139687	7.086006839025065	12.946079397041052	1.4967556725370104
8	77.16431359266652	3.9031678072914224	15.74945728749051	3.1830613125515583
9	44.25316562616089	18.776977597705617	21.98146122408379	14.988395552049694
10	28.07954096788292	19.091143748727095	30.312685205072114	22.516630078317867
11	24.415797386588853	16.10021641816134	38.4895073729158	20.994478822334
12	22.610488153682734	17.7537078524311	38.572112481873695	21.063691512012472
13	19.491777137917747	20.774531953006502	39.84492023036073	19.88877067871503
14	18.5782863315913	18.670921862457632	41.7800692460318	20.970722559919267
15	23.09611617062387	16.780062299756242	39.137094750810704	20.986726778809192
16	24.155145342480562	16.556003235297304	35.11395087204933	24.174900550172808
17	23.40494758201537	19.085642298483684	36.430020024723184	21.07939009477776
18	25.550902168377164	21.715863431776878	32.196542922009165	20.536691477836797
19	26.369062288920052	20.261896816688623	31.792353039731864	21.576687854659465
20	25.96020450946652	21.608890788154987	34.40723679658888	18.02366790578961
21	24.075846660689166	18.594818467423774	34.16697901752434	23.16235585436272
22	24.957718020667112	20.319217482608607	33.371046978773016	21.35201751795126
23	24.375314492625975	19.694802879981417	34.17425871431108	21.755623913081532
24	26.280288887265	18.576563655252453	32.780946977411546	22.362200480070996
25	21.88235176439567	20.31424394930775	32.61190241538672	25.19150187090986
26	20.49292994180966	21.07127684492384	34.536632017718006	23.899161195548494
27	21.16908040480671	21.5930532798785	34.448497673414465	22.789368641900325
28	23.88971426078708	21.958010597793695	32.690728750440044	21.461546390979176
29	22.426634132164285	19.839757758364634	35.66195644351803	22.071651665953052
30	26.959495711508396	20.226109605004208	31.676850369722466	21.137544313764934
31	27.5041948558106	20.838965605099787	30.94607439572265	20.71076514336696
32	28.00818882533201	19.911165471120025	30.06361954859767	22.017026154950294
33	23.462184892628642	22.19185001824092	33.265935937001174	21.08002915212927
34	23.714390265656142	23.514726520963443	31.522392986373355	21.248490227007064
35	27.631283913453853	24.422493596228563	28.52918727742397	19.417035212893623
36	25.295473723489888	24.110439112977282	28.52082396164989	22.073263201882938
37	24.39812606156456	27.50072171803067	27.821750789305295	20.279401431099476
38	23.812054900027814	23.84806439253014	28.423298252789415	23.91658245465263
39	23.555709546766636	21.66646151999513	32.45033204586431	22.327496887373922
40	21.74150908114389	21.986351402077936	32.08973698900071	24.182402527777462
41	22.342945404219055	25.29308420469729	28.282955701377947	24.081014689705704
42	21.23198587627683	21.525035349596326	29.670904913545264	27.572073860581582
43	22.37061936604955	21.081223911525566	32.8441580850063	23.703998637418586
44	24.671198046373892	23.08575232748855	30.183428909454186	22.05962071668337
45	24.94577042670415	23.772572269745552	30.18020583759441	21.101451465955886
46	23.11414870197727	24.35719860596585	29.845117504586625	22.68353518747025
47	22.671809874325206	24.945270294863843	30.691423933698076	21.69149589711288
48	24.221774017650763	23.982210866142214	30.448665495431992	21.347349620775034
49	22.604431001394534	23.21498083800424	31.395053862809945	22.78553429779128
50	22.6507487668277	22.639829221647595	32.74849397799587	21.96092803352884
51	22.377537856507175	21.158799916978115	32.09170973125971	24.371952495255
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1400.0
1	13183.0
2	24966.0
3	93096.5
4	161227.0
5	99358.5
6	37490.0
7	34339.5
8	31189.0
9	30653.0
10	30117.0
11	29755.5
12	29394.0
13	28085.0
14	26776.0
15	25077.0
16	23378.0
17	22469.5
18	21561.0
19	20193.0
20	18825.0
21	17904.0
22	16983.0
23	16850.0
24	16717.0
25	18425.5
26	22663.5
27	25193.0
28	27452.5
29	29712.0
30	32646.5
31	35581.0
32	40931.0
33	46281.0
34	54089.0
35	61897.0
36	66986.5
37	72076.0
38	77546.5
39	83017.0
40	91954.5
41	100892.0
42	112570.0
43	124248.0
44	132445.5
45	140643.0
46	150397.0
47	160151.0
48	180390.5
49	200630.0
50	203429.0
51	206228.0
52	200472.5
53	194717.0
54	195852.0
55	196987.0
56	201785.0
57	206583.0
58	211613.5
59	216644.0
60	217285.5
61	217927.0
62	212850.0
63	207773.0
64	197184.5
65	186596.0
66	161756.5
67	136917.0
68	113177.5
69	89438.0
70	76195.0
71	62952.0
72	60436.5
73	57921.0
74	48025.5
75	29092.5
76	20055.0
77	14982.0
78	9909.0
79	7679.0
80	5449.0
81	3894.5
82	2340.0
83	1703.5
84	1067.0
85	791.5
86	516.0
87	341.0
88	166.0
89	150.5
90	135.0
91	101.5
92	68.0
93	57.0
94	46.0
95	27.5
96	9.0
97	4.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3599051.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.245749226840484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.69910490869239	18.838730127661265
2	8.720937827354955	4.228913431698307
3	2.792556926706969	2.0312290483984063
4	1.4513828179290633	1.407594553426126
5	0.8962512944145749	1.0865142064303481
6	0.6258069615482005	0.9103895192465205
7	0.48086940453745447	0.8161327295292662
8	0.3984500058068597	0.7728575136181004
9	0.34588253331422397	0.7547563048212876
>10	4.934991938818259	29.513156113107293
>50	1.3512586579342087	22.5773520815699
>100	0.29392361299020187	10.170596471105252
>500	0.005762945253886269	0.9327218209697992
>1k	0.002084469559916289	1.0964698859249016
>5k	3.6784756939699215E-4	0.6360548020542072
>10k+	3.6784756939699215E-4	4.226531390439006
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	106098	2.9479437774013206	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	25284	0.7025185250222906	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10786	0.2996901127547234	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8703	0.241813744789946	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	7480	0.20783256475109688	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5212	0.14481595287202098	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4703	0.1306733358321402	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4399	0.12222666475134696	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	4127	0.11466911694221617	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03059139756563605	0.0
2	0.0	0.0	0.0	0.10374957176211173	0.0
3	0.0	0.0	0.0	0.1739347400189661	0.0
4	0.0	0.0	0.0	0.2208082074969207	0.0
5	0.0	0.0	0.0	0.4787095264835091	0.0
6	0.0	0.0	0.0	0.5857655254121156	0.0
7	0.0	0.0	0.0	0.881093377115245	0.0
8	0.0	0.0	0.0	1.2028726461503325	0.0
9	0.0	0.0	0.0	1.4922544859742193	0.0
10	0.0	0.0	0.0	1.6706070572492582	0.0
11	0.0	0.0	0.0	1.8434026080764068	0.0
12	0.0	0.0	0.0	1.9813834257975227	0.0
13	0.0	0.0	0.0	2.0565699124574786	0.0
14	0.0	0.0	0.0	2.1110564979490425	0.0
15	0.0	0.0	0.0	2.2609571245308833	0.0
16	0.0	0.0	0.0	2.432530130859496	0.0
17	0.0	0.0	0.0	2.666897468249269	2.7785102239451455E-5
18	0.0	0.0	0.0	2.7925417005760687	2.7785102239451455E-5
19	0.0	0.0	0.0	2.9380244959018365	2.7785102239451455E-5
20	0.0	0.0	0.0	3.1125982932723097	2.7785102239451455E-5
21	0.0	0.0	0.0	3.30203712034089	2.7785102239451455E-5
22	0.0	0.0	0.0	3.4788337258905195	2.7785102239451455E-5
23	0.0	0.0	0.0	3.640848657048761	2.7785102239451455E-5
24	5.557020447890291E-5	0.0	0.0	3.780857787233357	2.7785102239451455E-5
25	5.557020447890291E-5	0.0	0.0	3.921700470485136	2.7785102239451455E-5
26	5.557020447890291E-5	0.0	0.0	4.067961248673608	2.7785102239451455E-5
27	5.557020447890291E-5	0.0	0.0	4.225197142246665	2.7785102239451455E-5
28	5.557020447890291E-5	0.0	0.0	4.389712732606457	2.7785102239451455E-5
29	5.557020447890291E-5	0.0	0.0	4.566453767951607	2.7785102239451455E-5
30	5.557020447890291E-5	0.0	0.0	4.735414974669712	2.7785102239451455E-5
31	5.557020447890291E-5	0.0	0.0	4.916712766782132	2.7785102239451455E-5
32	5.557020447890291E-5	0.0	0.0	5.113320150228491	2.7785102239451455E-5
33	5.557020447890291E-5	0.0	0.0	5.301703143411972	2.7785102239451455E-5
34	5.557020447890291E-5	0.0	0.0	5.524595233576851	2.7785102239451455E-5
35	5.557020447890291E-5	0.0	0.0	5.716895926176095	2.7785102239451455E-5
36	5.557020447890291E-5	0.0	0.0	5.945122755970949	2.7785102239451455E-5
37	5.557020447890291E-5	0.0	0.0	6.203802057820242	2.7785102239451455E-5
38	5.557020447890291E-5	0.0	0.0	6.5038811620063175	2.7785102239451455E-5
39	5.557020447890291E-5	0.0	0.0	6.826382843699631	2.7785102239451455E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCGA	30	2.1667965E-6	44.999996	1
CGTTTTT	32875	0.0	43.774902	1
TATACGG	210	0.0	40.714287	1
CACTTAC	8025	0.0	40.542053	36
TTATCGG	145	0.0	40.34483	1
CGCACTT	8055	0.0	40.30726	34
ACCCGCA	8070	0.0	40.12082	31
CCGCACT	8095	0.0	40.0803	33
GCACTTA	8135	0.0	39.938538	35
ACTTACT	8115	0.0	39.926064	37
CTTACTG	8115	0.0	39.926064	38
CCCGCAC	8215	0.0	39.63177	32
ACTACGG	195	0.0	39.23077	1
TATGACC	8375	0.0	39.223877	27
AACGCAA	8615	0.0	39.123623	17
CAACGCA	8600	0.0	39.113373	16
TCAACGC	8630	0.0	39.08169	15
CGCAAGC	8660	0.0	38.998264	19
ATCAACG	8660	0.0	38.972282	14
TTATGAC	8470	0.0	38.916767	26
>>END_MODULE