FastQCFastQC Report
Thu 26 May 2016
SRR1043390_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043390_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2970474
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170063.938967316327293No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC226200.7614946301499357No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121070.4075780498331243No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC87880.2958450402191704No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC63570.21400624950765434No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50160.16886193920566211No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC40130.1350962842967149No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC38220.12866633405981673No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA31840.1071882803889211No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT335550.044.168531
CGGTCTA5050.041.88118430
TATACGG2100.041.7857131
GTTGATC22950.041.764715
TACGGGA4550.041.538463
CACTTAC65800.041.4437736
ACTTACT65950.041.1789237
CGCACTT66150.041.15646734
GCACTTA66150.041.15646735
TCACGAC5250.041.1428624
CTTACTG66100.041.05143738
CGTTTTA16200.040.9722251
CCGCACT66900.040.7959633
GGTACCT22200.040.7432447
TACGCGG3550.040.563381
CCCGCAC68100.040.53964632
CACGACG5350.040.37383325
ACCCGCA68000.040.3345631
TTATCGG451.9301297E-840.01
CATATGC23500.039.9255332