##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043390_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2970474
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.529862237474557	31.0	31.0	33.0	30.0	34.0
2	30.57000431580953	31.0	31.0	34.0	26.0	34.0
3	31.532069629291488	31.0	31.0	34.0	28.0	34.0
4	35.15537924250473	37.0	35.0	37.0	32.0	37.0
5	35.47180281665485	37.0	35.0	37.0	33.0	37.0
6	35.76950378963088	37.0	35.0	37.0	35.0	37.0
7	33.754804115437466	37.0	35.0	37.0	32.0	37.0
8	34.750655282624926	37.0	35.0	37.0	31.0	37.0
9	36.78804830474867	39.0	37.0	39.0	32.0	39.0
10	36.7426141417161	39.0	35.0	39.0	32.0	39.0
11	36.42755095651401	39.0	35.0	39.0	32.0	39.0
12	36.40480610165246	39.0	35.0	39.0	32.0	39.0
13	36.41605784127382	39.0	35.0	39.0	32.0	39.0
14	37.58995332058116	40.0	35.0	41.0	33.0	41.0
15	37.65227266759447	40.0	35.0	41.0	33.0	41.0
16	37.40932053268266	40.0	35.0	41.0	32.0	41.0
17	37.337788177913694	40.0	35.0	41.0	32.0	41.0
18	37.191608813946864	39.0	35.0	41.0	32.0	41.0
19	37.01402873750115	38.0	35.0	41.0	32.0	41.0
20	36.747295886111104	38.0	35.0	40.0	32.0	41.0
21	36.64173630201779	38.0	35.0	40.0	32.0	41.0
22	36.593728139010814	38.0	35.0	40.0	32.0	41.0
23	36.550255952417025	38.0	35.0	40.0	32.0	41.0
24	36.41537680518328	37.0	35.0	40.0	31.0	41.0
25	36.30597978639099	37.0	35.0	40.0	31.0	41.0
26	36.02819482681888	37.0	35.0	40.0	31.0	41.0
27	35.90287576999496	37.0	35.0	40.0	30.0	41.0
28	35.71394060341885	36.0	35.0	40.0	30.0	41.0
29	35.558916523086886	36.0	34.0	40.0	30.0	41.0
30	35.35180176631743	36.0	34.0	40.0	29.0	41.0
31	35.143233032842566	36.0	34.0	40.0	27.0	41.0
32	35.0091672238168	36.0	34.0	40.0	26.0	41.0
33	34.630119637472	36.0	33.0	40.0	24.0	41.0
34	34.42473793744702	36.0	33.0	40.0	23.0	41.0
35	34.33344442671439	36.0	33.0	40.0	23.0	41.0
36	34.25368375552185	36.0	34.0	40.0	23.0	41.0
37	34.13931412966416	36.0	34.0	40.0	22.0	41.0
38	34.017291516438114	35.0	33.0	40.0	22.0	41.0
39	33.91209820385568	35.0	33.0	40.0	21.0	41.0
40	33.83163158472352	35.0	33.0	40.0	21.0	41.0
41	33.74232933868467	35.0	33.0	40.0	21.0	41.0
42	33.624115208549206	35.0	33.0	40.0	21.0	41.0
43	33.45056007896383	35.0	33.0	40.0	20.0	41.0
44	33.207780643762575	35.0	33.0	40.0	19.0	41.0
45	32.99283885332778	35.0	33.0	39.0	20.0	41.0
46	32.97798903474664	35.0	33.0	39.0	20.0	41.0
47	32.96460463885562	35.0	33.0	39.0	20.0	41.0
48	32.875706368747885	35.0	33.0	39.0	20.0	41.0
49	32.829730204674405	35.0	33.0	39.0	20.0	41.0
50	32.77372230829154	35.0	33.0	39.0	20.0	41.0
51	32.35513221122285	35.0	32.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	7.0
12	10.0
13	26.0
14	89.0
15	167.0
16	388.0
17	927.0
18	1896.0
19	3404.0
20	5773.0
21	9413.0
22	14246.0
23	21298.0
24	32660.0
25	47838.0
26	62425.0
27	72659.0
28	78358.0
29	81866.0
30	90081.0
31	102837.0
32	122267.0
33	162283.0
34	301061.0
35	296795.0
36	295851.0
37	312601.0
38	380762.0
39	472239.0
40	241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.991904995633693	16.882053167272293	23.139169034975563	30.986872802118448
2	32.888050863262904	19.517726800503894	26.867496567887816	20.72672576834539
3	25.750940758949582	21.11982801398026	34.088869318499334	19.040361908570823
4	23.276992156807296	23.443867881018317	31.680263823214744	21.598876138959643
5	20.985842663494108	32.16900737054086	30.1991197364461	16.646030229518928
6	79.92549337243821	2.0546552503068534	16.52779994034622	1.4920514369087223
7	75.99006084550815	7.267459671419443	15.187407800909888	1.5550716821625101
8	75.04745033957543	3.5316922484425044	18.314955794933738	3.105901617048323
9	45.552460651061075	16.831589840543966	23.783544309763357	13.832405198631598
10	31.958636904413236	17.946058440504782	30.448978849840124	19.646325805241858
11	28.76399524116353	14.706036814326604	38.001544534643294	18.528423409866573
12	23.913018595685404	16.413676739806508	38.64921894620185	21.02408571830624
13	18.776902272162623	19.488707862785535	41.8934486549958	19.840941210056037
14	20.612972879075862	17.644490407928163	42.17882398566694	19.563712727329037
15	26.416861416730125	15.33230050153612	39.33382349079642	18.917014590937338
16	24.71097205361838	16.199468502333296	37.348854088606736	21.740705355441587
17	24.761805691616892	18.807368790300806	37.064791679711725	19.366033838370576
18	25.37409854454205	24.035995602048697	32.45125188774586	18.138653965663394
19	26.62753486480609	22.2573569066755	31.643603007466147	19.47150522105226
20	26.52987368345927	23.508941670588598	33.90482461721597	16.056360028736155
21	22.506340738885445	19.370645896917463	36.58089584355898	21.542117520638122
22	24.73362837042169	19.595357508599637	35.739413979048464	19.93160014193021
23	27.016664680451676	18.933409280808384	34.98455128710098	19.065374751638963
24	27.088942707460156	16.917535719888477	33.24651217280474	22.747009399846625
25	20.51689393679258	19.168758925343226	35.48026341923882	24.834083718625376
26	19.00198419511499	19.166301405095616	36.922356499333105	24.909357900456293
27	20.223304428855464	22.453184239283022	35.25528249026923	22.068228841592287
28	24.07437331550453	23.20114567574064	32.851558370818935	19.8729226379359
29	23.61771219004105	20.045083713912327	37.01560761009859	19.321596485948035
30	28.322146566507566	19.35071641764917	33.47630714828677	18.85082986755649
31	29.449845378212363	19.79471963060441	32.01465490019438	18.740780090988846
32	29.208604418015444	19.69409595909609	30.456721721853146	20.64057790103532
33	24.03087857358792	21.711854741027864	34.18154813002908	20.075718555355138
34	22.237494756729063	24.622804306652743	33.306603592557956	19.833097344060242
35	24.614253482777496	28.592271805779145	28.441588783473616	18.351885927969743
36	25.06293608360147	26.295096338160175	28.28073903356838	20.361228544669977
37	24.684982935383378	28.797121267514882	27.54213637284824	18.975759424253503
38	24.164291624838324	24.713429573865987	28.068382352446108	23.053896448849578
39	23.40209003680894	21.448933739194487	32.22808885046629	22.920887373530284
40	20.943021214796023	23.11661371215503	31.651884514053986	24.288480558994962
41	20.394085253733916	25.976123675884722	28.311474868993976	25.318316201387386
42	19.622794207254465	20.789746013599174	30.14535727294701	29.442102506199348
43	21.065594245228205	20.129985988768123	32.623076317113025	26.181343448890647
44	23.904703424436637	22.28371633618069	31.023567282527974	22.788012956854697
45	24.594660650118467	23.51658354861884	31.102881223670025	20.785874577592665
46	21.306229241528456	24.467172579191065	30.6389485314465	23.587649647833985
47	20.47733796020433	24.224652361878945	33.01930937621403	22.278700301702692
48	23.702614464896847	22.983469978192034	32.6042577716553	20.70965778525582
49	21.82143321234254	21.83971312322545	33.319699145658234	23.01915451877377
50	20.13018797673368	22.06772387167839	35.374691042574355	22.427397109013576
51	19.191718224094874	20.099923446561053	35.11712272182823	25.591235607515838
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1033.0
1	14154.0
2	27275.0
3	104449.0
4	181623.0
5	109957.0
6	38291.0
7	33784.5
8	29278.0
9	28928.5
10	28579.0
11	28802.0
12	29025.0
13	27732.5
14	26440.0
15	24694.5
16	22949.0
17	21159.5
18	19370.0
19	17970.5
20	16571.0
21	16267.5
22	15964.0
23	15499.0
24	15034.0
25	15465.0
26	16567.0
27	17238.0
28	19525.0
29	21812.0
30	24881.5
31	27951.0
32	30740.0
33	33529.0
34	38750.0
35	43971.0
36	46223.5
37	48476.0
38	50875.0
39	53274.0
40	59636.5
41	65999.0
42	75332.0
43	84665.0
44	92963.0
45	101261.0
46	113748.5
47	126236.0
48	145839.5
49	165443.0
50	165849.0
51	166255.0
52	154801.0
53	143347.0
54	146948.5
55	150550.0
56	157907.0
57	165264.0
58	175063.0
59	184862.0
60	192808.5
61	200755.0
62	199544.0
63	198333.0
64	182128.0
65	165923.0
66	137842.0
67	109761.0
68	89120.5
69	68480.0
70	59291.5
71	50103.0
72	47016.0
73	43929.0
74	37217.0
75	24108.5
76	17712.0
77	13087.5
78	8463.0
79	6688.5
80	4914.0
81	3660.5
82	2407.0
83	1695.5
84	984.0
85	721.0
86	458.0
87	298.5
88	139.0
89	109.0
90	79.0
91	62.5
92	46.0
93	30.0
94	14.0
95	9.5
96	5.0
97	4.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2970474.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.915965858878543
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.9862781785474	20.721600350559083
2	8.690141425343217	4.678070998267284
3	2.9107301123131477	2.350353369822912
4	1.5546072843665986	1.6737502635990102
5	1.0130038575195244	1.3632988621953894
6	0.7716833478218947	1.246236158630353
7	0.5911412152668072	1.113779573752815
8	0.4870439294162519	1.048740622075351
9	0.4172080619936919	1.0106602157403988
>10	5.554017588379207	34.537134813375864
>50	0.865846047425649	15.545744102269824
>100	0.14923301188292118	6.238738134400618
>500	0.0053329057198973	0.9485502422268217
>1k	0.0029330981459435154	1.3549128549827152
>5k	3.999679289922975E-4	0.7234790388353556
>10k+	3.999679289922975E-4	5.444950399266157
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	117006	3.938967316327293	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	22620	0.7614946301499357	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12107	0.4075780498331243	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8788	0.2958450402191704	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	6357	0.21400624950765434	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5016	0.16886193920566211	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4013	0.1350962842967149	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3822	0.12866633405981673	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	3184	0.1071882803889211	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04130653895640898	0.0
2	0.0	0.0	0.0	0.13260509938817847	0.0
3	0.0	0.0	0.0	0.21733905093934502	0.0
4	0.0	0.0	0.0	0.27409766926086543	0.0
5	0.0	0.0	0.0	0.6001735749917353	0.0
6	0.0	0.0	0.0	0.7498129928085552	0.0
7	0.0	0.0	0.0	1.1186766825765855	0.0
8	0.0	0.0	0.0	1.5490120431957997	0.0
9	0.0	0.0	0.0	1.960798175644695	0.0
10	0.0	0.0	0.0	2.203015411008479	0.0
11	0.0	0.0	0.0	2.4232832874484003	0.0
12	0.0	0.0	0.0	2.60833792855955	0.0
13	0.0	0.0	0.0	2.707312031682486	0.0
14	0.0	0.0	0.0	2.7791187534380035	0.0
15	0.0	0.0	0.0	2.9472400701032897	0.0
16	0.0	0.0	0.0	3.1370414284050288	0.0
17	0.0	0.0	0.0	3.39474440779485	0.0
18	0.0	0.0	0.0	3.540579718927013	0.0
19	0.0	0.0	0.0	3.716174590317909	0.0
20	0.0	0.0	0.0	3.909409744034117	0.0
21	6.732932185233738E-5	0.0	0.0	4.110152117136861	0.0
22	6.732932185233738E-5	0.0	0.0	4.309716227107189	0.0
23	6.732932185233738E-5	0.0	0.0	4.494333227626298	0.0
24	2.019879655570121E-4	0.0	0.0	4.651446200168727	0.0
25	2.019879655570121E-4	0.0	0.0	4.813878189137491	0.0
26	2.019879655570121E-4	0.0	0.0	4.981023230635919	0.0
27	2.019879655570121E-4	0.0	0.0	5.160455873372398	0.0
28	2.019879655570121E-4	0.0	0.0	5.3342328530732805	0.0
29	2.019879655570121E-4	0.0	0.0	5.524943157220026	0.0
30	2.019879655570121E-4	0.0	0.0	5.710738420871551	0.0
31	2.019879655570121E-4	0.0	0.0	5.905892460260551	0.0
32	2.019879655570121E-4	0.0	0.0	6.128651521609009	0.0
33	2.019879655570121E-4	0.0	0.0	6.3480104522039245	0.0
34	2.019879655570121E-4	0.0	0.0	6.605141132358001	0.0
35	2.019879655570121E-4	0.0	0.0	6.832747904879827	0.0
36	2.019879655570121E-4	0.0	0.0	7.082236707003664	0.0
37	2.019879655570121E-4	0.0	0.0	7.4337294317337905	0.0
38	2.019879655570121E-4	0.0	0.0	7.803973372599794	0.0
39	2.019879655570121E-4	0.0	0.0	8.235587990334203	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	33555	0.0	44.16853	1
CGGTCTA	505	0.0	41.881184	30
TATACGG	210	0.0	41.785713	1
GTTGATC	2295	0.0	41.7647	15
TACGGGA	455	0.0	41.53846	3
CACTTAC	6580	0.0	41.44377	36
ACTTACT	6595	0.0	41.17892	37
CGCACTT	6615	0.0	41.156467	34
GCACTTA	6615	0.0	41.156467	35
TCACGAC	525	0.0	41.14286	24
CTTACTG	6610	0.0	41.051437	38
CGTTTTA	1620	0.0	40.972225	1
CCGCACT	6690	0.0	40.79596	33
GGTACCT	2220	0.0	40.743244	7
TACGCGG	355	0.0	40.56338	1
CCCGCAC	6810	0.0	40.539646	32
CACGACG	535	0.0	40.373833	25
ACCCGCA	6800	0.0	40.33456	31
TTATCGG	45	1.9301297E-8	40.0	1
CATATGC	2350	0.0	39.92553	32
>>END_MODULE