FastQCFastQC Report
Thu 26 May 2016
SRR1043389_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043389_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences728934
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT397315.450562053628998No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42500.5830431836078438No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16710.22923886113146047No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8790.12058704903324582No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7480.10261560031498052No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT7370.10110654736917198No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTAT253.889396E-545.00000431
CGGAACG253.889396E-545.00000434
AGCCGGC253.889396E-545.00000433
ACCGGTC253.889396E-545.00000440
TTAATCG1000.045.00000419
TACCATA302.1644046E-645.00000436
GCGAAAC253.889396E-545.0000049
CGGCCCA253.889396E-545.00000436
GTCCTCA253.889396E-545.00000438
CGGCATA253.889396E-545.0000045
CGTGACA302.1644046E-645.00000428
CGGCACT253.889396E-545.00000439
GTTAGTG253.889396E-545.00000413
TAACGTA253.889396E-545.00000418
GAATACG253.889396E-545.00000425
ACGGGAT2150.045.0000044
TACGCAG253.889396E-545.00000428
ACGGCAC253.889396E-545.00000438
CGGATTA302.1644046E-645.0000044
GTTCGCA253.889396E-545.00000410