##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043389_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	728934
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.625457174449263	31.0	31.0	34.0	30.0	34.0
2	30.724172558832485	31.0	31.0	34.0	26.0	34.0
3	31.599136547341736	31.0	31.0	34.0	28.0	34.0
4	35.19160308066299	37.0	35.0	37.0	33.0	37.0
5	35.50182595406443	37.0	35.0	37.0	33.0	37.0
6	35.86016841030875	37.0	35.0	37.0	35.0	37.0
7	33.97250231159474	37.0	35.0	37.0	32.0	37.0
8	34.918478216134794	37.0	35.0	37.0	32.0	37.0
9	36.922950500319644	39.0	37.0	39.0	32.0	39.0
10	36.820177135378515	39.0	35.0	39.0	32.0	39.0
11	36.35517481692444	38.0	35.0	39.0	32.0	39.0
12	36.155304869851044	38.0	35.0	39.0	32.0	39.0
13	36.211219397092194	39.0	35.0	39.0	32.0	39.0
14	37.55573481275397	40.0	35.0	41.0	33.0	41.0
15	37.75256196034209	40.0	35.0	41.0	33.0	41.0
16	37.73241610351555	40.0	35.0	41.0	33.0	41.0
17	37.70971583161164	40.0	36.0	41.0	33.0	41.0
18	37.435265744223756	39.0	36.0	41.0	33.0	41.0
19	37.15724194508694	38.0	35.0	41.0	33.0	41.0
20	36.86019310390241	38.0	35.0	40.0	33.0	41.0
21	36.77198209988833	38.0	35.0	40.0	33.0	41.0
22	36.69455122137258	37.0	35.0	40.0	33.0	41.0
23	36.60579147083275	37.0	35.0	40.0	33.0	41.0
24	36.431631670356985	37.0	35.0	40.0	32.0	41.0
25	36.33561063141519	36.0	35.0	40.0	32.0	41.0
26	36.09134023107716	36.0	35.0	40.0	31.0	41.0
27	35.938139804152364	36.0	35.0	40.0	31.0	41.0
28	35.89722389132624	36.0	35.0	40.0	31.0	41.0
29	35.85326929461378	36.0	35.0	40.0	31.0	41.0
30	35.6877755736459	36.0	35.0	40.0	31.0	41.0
31	35.28319024767674	35.0	35.0	40.0	30.0	41.0
32	35.109909813508494	35.0	34.0	40.0	29.0	41.0
33	34.917239420852916	35.0	34.0	40.0	27.0	41.0
34	34.75929233648039	35.0	34.0	40.0	25.0	41.0
35	34.55409817624092	35.0	34.0	40.0	24.0	41.0
36	34.3732889397394	35.0	34.0	40.0	23.0	41.0
37	34.2770003868663	35.0	34.0	40.0	23.0	41.0
38	34.18633237028318	35.0	34.0	40.0	23.0	41.0
39	33.985503488656036	35.0	34.0	40.0	22.0	41.0
40	33.950682777864664	35.0	34.0	40.0	22.0	41.0
41	33.88374256105491	35.0	33.0	40.0	21.0	41.0
42	33.77506879909566	35.0	33.0	40.0	21.0	41.0
43	33.61672661722461	35.0	33.0	40.0	21.0	41.0
44	33.42022323008668	35.0	33.0	40.0	19.0	41.0
45	33.274027552563055	35.0	33.0	40.0	19.0	41.0
46	33.25766804676418	35.0	33.0	40.0	20.0	41.0
47	33.255789961779804	35.0	33.0	40.0	20.0	41.0
48	33.160731972990696	35.0	33.0	39.0	19.0	41.0
49	33.043639067460155	35.0	33.0	39.0	18.0	41.0
50	32.93289653109884	35.0	33.0	39.0	18.0	41.0
51	32.424091893093205	35.0	32.0	39.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	0.0
12	1.0
13	11.0
14	16.0
15	31.0
16	90.0
17	169.0
18	319.0
19	702.0
20	1096.0
21	1911.0
22	3103.0
23	4971.0
24	7818.0
25	12005.0
26	15182.0
27	16457.0
28	16559.0
29	17126.0
30	18753.0
31	22412.0
32	28076.0
33	37900.0
34	71573.0
35	102704.0
36	76461.0
37	64206.0
38	80884.0
39	128309.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.148869444970323	17.76676626416109	24.964262882510624	31.120101408357957
2	31.11584862278341	20.692545552820967	29.010033830223314	19.181571994172312
3	21.781533033168984	21.838602671846836	38.31965582617905	18.06020846880513
4	23.399237791075738	24.13291189600156	34.62782638757418	17.84002392534852
5	19.496003753426237	31.94061465098349	31.607113949959803	16.95626764563047
6	79.23803801167185	2.0086866575026	17.301703583589187	1.4515717472363754
7	76.17452334504907	6.5464088655488695	15.643117209514168	1.635950579887891
8	75.2301305742358	3.867839886738717	18.355708472920732	2.54632106610475
9	42.91389892637743	23.63794252977636	23.475513558154784	9.972644985691433
10	34.80795243465115	21.552294172037524	33.36186815267226	10.27788524063907
11	31.956802673493073	21.984157687801638	33.56586467361928	12.493174965086002
12	18.125783678632086	34.129976102088804	33.54295450616928	14.20128571310983
13	13.031083746951028	37.0886801822936	34.52054644179034	15.359689628965038
14	11.452339992372423	25.011592270356438	47.917232561521345	15.618835175749792
15	13.159353247344752	21.614714089341422	46.55881053703079	18.667122126283036
16	14.608867195109571	22.13849264816842	35.015927367909846	28.236712788812156
17	17.335863054817032	22.97587984646072	41.70418721036472	17.98406988835752
18	20.717650706373966	25.07661873365764	35.476188516381455	18.729542043586935
19	22.776136111088245	24.603873601725258	34.279509530355284	18.34048075683121
20	16.188571256108233	29.6113228358123	36.71114257257859	17.48896333550088
21	18.04786167197579	25.824560248252926	34.33452136956158	21.793056710209704
22	13.448405479782807	29.636565175996733	33.541994199749226	23.373035144471242
23	13.48750366974239	24.0167148191743	47.02812600317724	15.467655507906066
24	14.265351869990972	26.121432118682897	42.36487802736599	17.248337983960138
25	14.029253677287656	32.98048931727701	35.14186469556915	17.84839230986619
26	14.087832368911316	33.19546076873901	34.85473307597121	17.861973786378467
27	13.021755056013301	29.853182867035972	41.71982648634856	15.405235590602167
28	12.513067026644386	25.531255230240323	39.78000751782741	22.175670225287885
29	14.50199880922004	31.64854431265382	37.47870177546939	16.370755102656755
30	21.082155586102445	29.44422951872186	33.658328463207916	15.81528643196778
31	21.353647929716544	28.1002120905322	34.7556843280736	15.790455651677654
32	19.751170887899317	31.560058935376865	32.91326786787281	15.775502308851008
33	15.533230717733018	29.55932910249762	34.735106333358026	20.172333846411338
34	15.092450070925489	29.750155706826682	36.16952975166476	18.98786447058307
35	22.805905610110106	28.179231590239993	32.021006017005654	16.993856782644244
36	17.238872106390975	32.78376368779615	32.64122677773296	17.33613742807991
37	17.54932545333322	31.654168964542745	32.74740923046531	18.049096351658726
38	17.382369322874226	32.93988207437162	32.35834794370958	17.31940065904458
39	20.240927162129903	29.128563079785003	31.13491756455317	19.495592193531923
40	14.556599088532021	28.434947471238825	33.41070659346388	23.597746846765276
41	17.684454285298806	28.361278250157078	31.583792222615493	22.370475241928624
42	17.29072865307422	28.30571766442504	34.054386268166944	20.349167414333806
43	16.67805315707595	30.94203316075255	33.29437233000518	19.085541352166313
44	14.191957022172103	37.12105622731276	30.04743913715096	18.639547613364176
45	17.86650094521589	31.830453785939465	32.022789443214336	18.280255825630302
46	17.563181303108376	31.720292920895442	30.553932180416883	20.1625935955793
47	17.48704272266076	28.120378525353463	34.61671427042777	19.775864481558
48	18.520195244013863	27.333201634167153	34.81083335391133	19.335769767907657
49	18.652305970087827	31.633042223301423	31.726191946047244	17.98845986056351
50	16.453753014676227	30.760398060729777	31.484331914823567	21.301517009770432
51	16.003506490299532	27.964397325409436	35.00440369086913	21.027692493421902
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	341.0
1	3673.5
2	7006.0
3	27672.5
4	48339.0
5	28238.5
6	8138.0
7	8312.5
8	8487.0
9	9923.0
10	11359.0
11	12708.0
12	14057.0
13	13868.5
14	13680.0
15	12410.5
16	11141.0
17	9868.0
18	8595.0
19	7970.0
20	7345.0
21	6970.0
22	6595.0
23	5998.0
24	5401.0
25	5408.0
26	6112.0
27	6809.0
28	6967.0
29	7125.0
30	8141.0
31	9157.0
32	10849.5
33	12542.0
34	15487.0
35	18432.0
36	20872.5
37	23313.0
38	24786.0
39	26259.0
40	30892.0
41	35525.0
42	45235.0
43	54945.0
44	61293.0
45	67641.0
46	66504.5
47	65368.0
48	59748.0
49	54128.0
50	47855.0
51	41582.0
52	37654.5
53	33727.0
54	29391.0
55	25055.0
56	24277.5
57	23500.0
58	21825.5
59	20151.0
60	17013.5
61	13876.0
62	11685.5
63	9495.0
64	7948.0
65	6401.0
66	5562.5
67	4724.0
68	4107.5
69	3491.0
70	2990.0
71	2489.0
72	2356.0
73	2223.0
74	1976.5
75	1654.5
76	1579.0
77	1221.5
78	864.0
79	672.0
80	480.0
81	375.5
82	271.0
83	171.0
84	71.0
85	43.0
86	15.0
87	17.0
88	19.0
89	15.5
90	12.0
91	12.0
92	12.0
93	9.0
94	6.0
95	9.0
96	12.0
97	8.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	728934.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.19179384234069
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.48439707656175	26.05037927605644
2	9.435211499250688	6.26343189884022
3	3.510311772723862	3.495406340477757
4	1.9071264118945335	2.5320378677954505
5	1.1897650192272347	1.9745217619509439
6	0.7826234862206577	1.5586006446486018
7	0.5669433928045611	1.3172507749972318
8	0.4092970575317388	1.0868242843096105
9	0.2973530267119478	0.8882712324917096
>10	2.4522460544683797	17.1683456310491
>50	0.6320613396643723	14.947301333702823
>100	0.3289213944821972	15.850237196826564
>500	0.002494978972052065	0.5664397413402782
>1k	8.316596573506882E-4	0.8172245878352309
>5k	0.0	0.0
>10k+	4.158298286753441E-4	5.48372742767802
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39731	5.450562053628998	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4250	0.5830431836078438	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1671	0.22923886113146047	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	879	0.12058704903324582	No Hit
CTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	748	0.10261560031498052	No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATT	737	0.10110654736917198	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09726532168893205	0.0
2	0.0	0.0	0.0	0.30647493463057013	0.0
3	0.0	0.0	0.0	0.5058071101087341	0.0
4	0.0	0.0	0.0	0.6465605939632395	0.0
5	0.0	0.0	0.0	1.3984805208702022	0.0
6	0.0	0.0	0.0	1.6345787135735197	0.0
7	0.0	0.0	0.0	2.3318983611685007	0.0
8	0.0	0.0	0.0	3.0869735805985177	0.0
9	0.0	0.0	0.0	3.717208965420738	0.0
10	0.0	0.0	0.0	4.10174309333904	0.0
11	0.0	0.0	0.0	4.516732653436388	0.0
12	0.0	0.0	0.0	4.885764692002294	0.0
13	0.0	0.0	0.0	5.047782103729555	0.0
14	0.0	0.0	0.0	5.173033498231664	0.0
15	0.0	0.0	0.0	5.591727097377815	0.0
16	0.0	0.0	0.0	6.057064151212592	0.0
17	0.0	0.0	0.0	6.70280162538721	0.0
18	0.0	0.0	0.0	6.999810682448617	0.0
19	0.0	0.0	0.0	7.344423500618712	0.0
20	0.0	0.0	0.0	7.71139773971306	0.0
21	0.0	0.0	0.0	8.087289109850824	0.0
22	1.3718663143713971E-4	0.0	0.0	8.48115192870685	0.0
23	1.3718663143713971E-4	0.0	0.0	8.81067421741886	0.0
24	0.0017834262086828163	0.0	0.0	9.084773107030266	0.0
25	0.0017834262086828163	0.0	0.0	9.331571856985681	0.0
26	0.0017834262086828163	0.0	0.0	9.609923532171637	0.0
27	0.0017834262086828163	0.0	0.0	9.87839776989412	0.0
28	0.0017834262086828163	0.0	0.0	10.179522425898641	0.0
29	0.0017834262086828163	0.0	0.0	10.568446526022932	0.0
30	0.0017834262086828163	0.0	0.0	10.883838591696916	0.0
31	0.0017834262086828163	0.0	0.0	11.170695838031975	0.0
32	0.0017834262086828163	0.0	0.0	11.472917987087994	0.0
33	0.0017834262086828163	0.0	0.0	11.788858799287727	0.0
34	0.0017834262086828163	0.0	0.0	12.159262704168004	0.0
35	0.0017834262086828163	0.0	0.0	12.477124129207857	0.0
36	0.0017834262086828163	0.0	0.0	12.778248785212378	0.0
37	0.0017834262086828163	0.0	0.0	13.113807285707622	0.0
38	0.0017834262086828163	0.0	0.0	13.441683334842386	0.0
39	0.0017834262086828163	0.0	0.0	13.774772475971762	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGCTAT	25	3.889396E-5	45.000004	31
CGGAACG	25	3.889396E-5	45.000004	34
AGCCGGC	25	3.889396E-5	45.000004	33
ACCGGTC	25	3.889396E-5	45.000004	40
TTAATCG	100	0.0	45.000004	19
TACCATA	30	2.1644046E-6	45.000004	36
GCGAAAC	25	3.889396E-5	45.000004	9
CGGCCCA	25	3.889396E-5	45.000004	36
GTCCTCA	25	3.889396E-5	45.000004	38
CGGCATA	25	3.889396E-5	45.000004	5
CGTGACA	30	2.1644046E-6	45.000004	28
CGGCACT	25	3.889396E-5	45.000004	39
GTTAGTG	25	3.889396E-5	45.000004	13
TAACGTA	25	3.889396E-5	45.000004	18
GAATACG	25	3.889396E-5	45.000004	25
ACGGGAT	215	0.0	45.000004	4
TACGCAG	25	3.889396E-5	45.000004	28
ACGGCAC	25	3.889396E-5	45.000004	38
CGGATTA	30	2.1644046E-6	45.000004	4
GTTCGCA	25	3.889396E-5	45.000004	10
>>END_MODULE