FastQCFastQC Report
Thu 26 May 2016
SRR1043388_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043388_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences23966
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7243.020946340649253No Hit
CGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG2961.2350830342985897No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC2821.1766669448385214No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA1930.805307518985229No Hit
CGGTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT920.3838771593090211No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC760.3171159142118No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGCT730.3045981807560711Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGCTT540.22531920220312107Illumina Single End Adapter 1 (95% over 23bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC510.21280146874739211No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC490.2044563131102395No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC470.19611115747308686No Hit
CGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC410.17107569056162897Illumina Single End Adapter 1 (95% over 21bp)
GATCCTATTGTCATCATGGGCCGCCGCCCCGCCCGGTGTTACCGGTATTGTA350.14604022365017108No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGCT250.1043144454644079Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGAC1050.046.00000426
TGGCAGG359.6466465E-846.0000041
AGCTTAT1050.046.00000423
ACGCAAG1050.046.00000418
TATGACC1050.046.00000427
CTTATGA1050.046.00000425
AAGCTTA1050.046.00000422
CGCAAGC1050.046.00000419
GCTTATG1050.046.00000424
GCGGGAA206.18788E-446.04
AGGGGGC253.317319E-546.04
GCAAGCT1100.046.020
TTACTGG1100.046.039
TGACCCG1100.046.029
TACGGCG253.317319E-546.014
ACCCGCA1100.046.031
CCGCACT1100.046.033
CACTTAC1100.046.036
GAATTCC1100.046.046
GTAGGGC206.18788E-446.03
Produced by FastQC (version 0.11.5)