##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043388_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	23966
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31002253192022	33.0	31.0	34.0	31.0	34.0
2	32.64954518901777	34.0	31.0	34.0	31.0	34.0
3	32.862346657765166	34.0	31.0	34.0	31.0	34.0
4	36.233330551614785	37.0	37.0	37.0	35.0	37.0
5	36.18843361428691	37.0	37.0	37.0	35.0	37.0
6	36.27309521822582	37.0	37.0	37.0	35.0	37.0
7	36.24146707836101	37.0	37.0	37.0	35.0	37.0
8	36.239547692564464	37.0	37.0	37.0	35.0	37.0
9	37.8291329383293	39.0	38.0	39.0	35.0	39.0
10	37.63089376616874	39.0	37.0	39.0	35.0	39.0
11	37.263289660352164	39.0	37.0	39.0	35.0	39.0
12	37.24313610948844	39.0	37.0	39.0	35.0	39.0
13	37.20829508470333	39.0	37.0	39.0	35.0	39.0
14	38.56471668196612	40.0	37.0	41.0	35.0	41.0
15	38.60948844195944	40.0	37.0	41.0	35.0	41.0
16	38.39956605190687	40.0	37.0	41.0	34.0	41.0
17	38.38754902778937	40.0	37.0	41.0	34.0	41.0
18	38.235750646749565	40.0	37.0	41.0	34.0	41.0
19	38.028373529166316	40.0	36.0	41.0	34.0	41.0
20	37.81736626888092	40.0	35.0	41.0	34.0	41.0
21	37.76020195276642	40.0	35.0	41.0	34.0	41.0
22	37.64061587248602	39.0	35.0	41.0	34.0	41.0
23	37.70495702244847	39.0	35.0	41.0	34.0	41.0
24	37.58862555286656	39.0	35.0	41.0	34.0	41.0
25	37.432445965117246	39.0	35.0	41.0	34.0	41.0
26	37.2904531419511	39.0	35.0	41.0	33.0	41.0
27	37.35600433948093	39.0	35.0	41.0	33.0	41.0
28	37.28523741967788	39.0	35.0	41.0	33.0	41.0
29	37.22160560794459	39.0	35.0	41.0	33.0	41.0
30	37.134607360427275	39.0	35.0	41.0	33.0	41.0
31	37.071517983810395	39.0	35.0	41.0	33.0	41.0
32	36.80697655011266	39.0	35.0	41.0	32.0	41.0
33	36.45890010848702	39.0	35.0	41.0	31.0	41.0
34	36.21038137361262	39.0	35.0	41.0	30.0	41.0
35	36.02883251272636	39.0	35.0	41.0	30.0	41.0
36	35.976883918885086	39.0	35.0	41.0	30.0	41.0
37	35.94083284653259	38.0	35.0	41.0	30.0	41.0
38	35.81223399816407	38.0	35.0	41.0	30.0	41.0
39	35.76954852708003	38.0	35.0	41.0	30.0	41.0
40	35.68993574230159	38.0	35.0	41.0	30.0	41.0
41	35.60606692814821	38.0	35.0	41.0	29.0	41.0
42	35.52507719268964	38.0	35.0	41.0	29.0	41.0
43	35.406617708420264	37.0	35.0	40.0	29.0	41.0
44	35.18576316448302	37.0	35.0	40.0	28.0	41.0
45	35.10398063923892	37.0	35.0	40.0	27.0	41.0
46	35.01835934240174	36.0	35.0	40.0	26.0	41.0
47	34.92159726278895	36.0	35.0	40.0	27.0	41.0
48	34.83180338813319	36.0	35.0	40.0	26.0	41.0
49	34.82458482850705	36.0	35.0	40.0	27.0	41.0
50	34.75678043895519	35.0	35.0	40.0	27.0	41.0
51	34.631311023950595	35.0	35.0	40.0	27.0	41.0
52	34.250855378452805	35.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	6.0
19	14.0
20	30.0
21	34.0
22	63.0
23	80.0
24	128.0
25	218.0
26	329.0
27	404.0
28	447.0
29	388.0
30	408.0
31	436.0
32	529.0
33	713.0
34	1884.0
35	2493.0
36	1931.0
37	2350.0
38	3589.0
39	7469.0
40	21.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.327630810314613	16.994909455061336	22.924142535258284	31.753317199365767
2	35.14145038804974	19.26896436618543	23.295501961111576	22.29408328465326
3	28.56964032379204	20.804473003421514	30.5849954101644	20.040891262622047
4	23.11608111491279	23.412334140031714	29.696236334807647	23.77534841024785
5	21.12576149545189	32.65459400817825	27.24693315530335	18.97271134106651
6	81.1649837269465	1.9193857965451053	14.512225653008429	2.403404823499958
7	80.21780856212969	1.886005173996495	14.094967871150796	3.8012183927230243
8	72.0562463489944	3.187849453392306	17.47892848201619	7.276975715597096
9	42.93999833096887	16.07276975715597	22.71134106651089	18.275890845364266
10	29.996661937745138	16.081114912793122	32.003671868480346	21.91855128098139
11	28.340148543770344	12.42593674372027	37.92038721522156	21.313527497287822
12	22.715513644329466	13.594258532921637	39.914879412501044	23.77534841024785
13	17.428857548193275	19.36076107819411	40.87874488859217	22.331636485020447
14	17.82525244095802	17.87532337478094	41.81757489777184	22.48184928648919
15	23.257948760744387	14.583159475924226	38.99691229241425	23.16197947091713
16	20.733539180505716	18.088124843528334	36.322289910706836	24.856046065259115
17	22.41508804139197	21.12576149545189	34.18175748977718	22.277392973378955
18	22.36084452975048	27.08420262037887	30.159392472669616	20.395560377201036
19	24.267712592839857	22.39422515229909	30.46399065342569	22.874071601435368
20	25.106400734373697	25.80739380789452	31.398648084786778	17.687557372945008
21	19.782191437870317	19.42752232329133	33.309688725694734	27.480597513143618
22	22.37336226320621	22.594508887590752	32.0996411583076	22.932487690895435
23	25.949261453726113	20.02837352916632	32.45848285070517	21.563882166402404
24	26.954852708003003	19.769673704414586	28.686472502712174	24.589001084870233
25	20.395560377201036	19.102061253442375	32.50020862889093	28.00216974046566
26	20.237002420095134	17.87115079696236	33.52249019444213	28.369356588500377
27	22.552783109404988	20.512392556121174	32.4876908954352	24.447133439038637
28	24.05073854627389	23.39147125093883	27.8185763164483	24.73921388633898
29	23.691896853876322	19.001919385796544	32.38754902778937	24.91863473253776
30	27.79771342735542	20.41225068847534	28.08144871901861	23.70858716515063
31	27.99382458482851	21.563882166402404	26.25803221230076	24.184261036468328
32	26.74205123925561	20.87540682633731	25.594592339147127	26.787949595259953
33	21.935241592255693	22.52774764249353	30.480680964699992	25.05632980055078
34	22.94917800216974	24.054911124092467	28.46949845614621	24.52641241759159
35	24.413752816490028	28.628056413252107	25.22323291329383	21.734957856964034
36	24.192606192105483	26.328966035216556	25.565384294417093	23.91304347826087
37	24.29692063756989	28.87423850454811	25.811566385713093	21.017274472168907
38	25.302511891846784	22.339981640657598	24.497204372861553	27.860302094634065
39	25.498623049319868	19.611115747308688	30.797796878911793	24.09246432445965
40	19.490110990569974	21.484603187849455	31.732454310272885	27.292831511307686
41	20.658432779771342	25.11474589001085	27.367937912042063	26.85888341817575
42	20.97972127180172	17.996328131519654	28.440290411416175	32.58366018526245
43	23.02428440290411	19.990820328799135	29.64616540098473	27.338729867312022
44	24.609863973963115	20.307936243010932	29.604439622798967	25.477760160226985
45	26.516732037052492	21.99783025953434	27.9771342735542	23.50830342985897
46	23.54168405240758	20.178586330635067	30.322123007594094	25.957606609363264
47	22.16890595009597	22.673787866143705	30.480680964699992	24.676625219060337
48	22.93666026871401	20.37052491028958	29.550196111157472	27.14261870983894
49	21.438704831845115	17.65834932821497	33.53083535007928	27.372110489860635
50	20.725194024868564	18.70149378285905	34.08996077776851	26.48335141450388
51	19.644496369857297	16.64024034048235	33.747809396645245	29.967453893015104
52	18.84753400650922	18.25085537845281	34.89944087457231	28.00216974046566
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	25.0
2	48.0
3	549.5
4	1051.0
5	622.0
6	193.0
7	163.5
8	134.0
9	138.0
10	142.0
11	136.5
12	131.0
13	134.0
14	120.5
15	104.0
16	106.0
17	108.0
18	89.0
19	70.0
20	79.0
21	88.0
22	81.0
23	74.0
24	77.5
25	81.0
26	78.0
27	75.0
28	79.0
29	83.0
30	101.0
31	119.0
32	136.5
33	154.0
34	174.0
35	194.0
36	212.0
37	230.0
38	259.5
39	302.0
40	315.0
41	410.0
42	505.0
43	614.5
44	724.0
45	898.5
46	1073.0
47	1313.5
48	1554.0
49	1814.0
50	2074.0
51	2214.0
52	2354.0
53	1880.0
54	1406.0
55	1408.0
56	1410.0
57	1353.0
58	1296.0
59	1377.0
60	1458.0
61	1479.0
62	1500.0
63	1467.0
64	1234.5
65	1035.0
66	848.5
67	662.0
68	570.0
69	478.0
70	418.0
71	358.0
72	374.5
73	391.0
74	304.0
75	217.0
76	161.0
77	105.0
78	81.5
79	58.0
80	45.0
81	32.0
82	22.5
83	13.0
84	9.0
85	5.0
86	3.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	23966.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.97579904865225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.64538008034643	72.49436701994493
2	7.246830489904524	11.591421180005007
3	1.1895445296603537	2.8540432279062005
4	0.3443418375332603	1.1015605441041476
5	0.14086711535451557	0.5632980055078027
6	0.08347680909897219	0.4005674705833264
7	0.036521103980800336	0.2044563131102395
8	0.05217300568685762	0.3338062254861053
9	0.036521103980800336	0.2628724025703079
>10	0.1773882193353159	2.5118918467829423
>50	0.02608650284342881	1.4437119252274053
>100	0.015651901706057285	3.2170574981223403
>500	0.005217300568685762	3.020946340649253
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	724	3.020946340649253	No Hit
CGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG	296	1.2350830342985897	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	282	1.1766669448385214	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	193	0.805307518985229	No Hit
CGGTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT	92	0.3838771593090211	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	76	0.3171159142118	No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGCT	73	0.3045981807560711	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGCTT	54	0.22531920220312107	Illumina Single End Adapter 1 (95% over 23bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	51	0.21280146874739211	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	49	0.2044563131102395	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	47	0.19611115747308686	No Hit
CGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	41	0.17107569056162897	Illumina Single End Adapter 1 (95% over 21bp)
GATCCTATTGTCATCATGGGCCGCCGCCCCGCCCGGTGTTACCGGTATTGTA	35	0.14604022365017108	No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGCT	25	0.1043144454644079	Illumina Single End Adapter 1 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.23783693565885003	0.0
2	0.0	0.0	0.0	0.7468914295251606	0.0
3	0.0	0.0	0.0	1.2726362346657765	0.0
4	0.0	0.0	0.0	2.787281982808979	0.0
5	0.0	0.0	0.0	4.794291913544187	0.0
6	0.0	0.0	0.0	5.511975298339314	0.0
7	0.0	0.0	0.0	7.460569139614454	0.0
8	0.0	0.0	0.0	9.45923391471251	0.0
9	0.0	0.0	0.0	11.921054827672537	0.0
10	0.0	0.0	0.0	12.96002670449804	0.0
11	0.0	0.0	0.0	13.965617958774931	0.0
12	0.0	0.0	0.0	14.583159475924226	0.0
13	0.0	0.0	0.0	14.937828590503212	0.0
14	0.0	0.0	0.0	15.113076858883419	0.0
15	0.0	0.0	0.0	15.538679796378203	0.0
16	0.0	0.0	0.0	15.955937578235835	0.0
17	0.0	0.0	0.0	16.60268714011516	0.0
18	0.0	0.0	0.0	16.869732120504047	0.0
19	0.0	0.0	0.0	17.257781857631645	0.0
20	0.0	0.0	0.0	17.499791371109072	0.0
21	0.0	0.0	0.0	17.81690728532087	0.0
22	0.0	0.0	0.0	18.121505466076943	0.0
23	0.0	0.0	0.0	18.426103646833013	0.0
24	0.0	0.0	0.0	18.626387382124676	0.0
25	0.0	0.0	0.0	18.88508720687641	0.0
26	0.0	0.0	0.0	19.13544187599099	0.0
27	0.0	0.0	0.0	19.39414170074272	0.0
28	0.0	0.0	0.0	19.648668947675873	0.0
29	0.0	0.0	0.0	19.899023616790455	0.0
30	0.0	0.0	0.0	20.157723441542185	0.0
31	0.0	0.0	0.0	20.399732955019612	0.0
32	0.0	0.0	0.0	20.666777935408497	0.0
33	0.0	0.0	0.0	20.879579404155887	0.0
34	0.0	0.0	0.0	21.288492030376368	0.0
35	0.0	0.0	0.0	21.51381123257949	0.0
36	0.0	0.0	0.0	21.814236835516983	0.0
37	0.0	0.0	0.0	22.177251105733124	0.0
38	0.0	0.0	0.0	22.5861637319536	0.0
39	0.0	0.0	0.0	23.02845698072269	0.0
40	0.0	0.0	0.0	23.383126095301677	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATGAC	105	0.0	46.000004	26
TGGCAGG	35	9.6466465E-8	46.000004	1
AGCTTAT	105	0.0	46.000004	23
ACGCAAG	105	0.0	46.000004	18
TATGACC	105	0.0	46.000004	27
CTTATGA	105	0.0	46.000004	25
AAGCTTA	105	0.0	46.000004	22
CGCAAGC	105	0.0	46.000004	19
GCTTATG	105	0.0	46.000004	24
GCGGGAA	20	6.18788E-4	46.0	4
AGGGGGC	25	3.317319E-5	46.0	4
GCAAGCT	110	0.0	46.0	20
TTACTGG	110	0.0	46.0	39
TGACCCG	110	0.0	46.0	29
TACGGCG	25	3.317319E-5	46.0	14
ACCCGCA	110	0.0	46.0	31
CCGCACT	110	0.0	46.0	33
CACTTAC	110	0.0	46.0	36
GAATTCC	110	0.0	46.0	46
GTAGGGC	20	6.18788E-4	46.0	3
>>END_MODULE