FastQCFastQC Report
Thu 26 May 2016
SRR1043387_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043387_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences23589
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7032.9802026368222476No Hit
CGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT2931.2421043706812498No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC2531.0725338081309086No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG1320.5595828564161262No Hit
CGGTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC830.3518589172919581No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC600.2543558438255119No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC580.2458773156979948No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC570.2416380516342363Illumina Single End Adapter 1 (95% over 21bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC520.22044173131544365No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGCT500.21196320318792658Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG500.21196320318792658No Hit
GATCCTATTGTCATCATGGGCCGCCGCCCCGCCCGGTGTTACCGGTATTGT370.15685277035906567No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC370.15685277035906567No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC270.11446012972148034Illumina Single End Adapter 1 (95% over 21bp)
GGGCCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC260.11022086565772182No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC260.11022086565772182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACTT302.0760235E-645.00000414
TCGATCA302.0760235E-645.00000416
CAACTTC302.0760235E-645.00000415
CTGGGCC302.0760235E-645.0000049
TCGGCGT302.0760235E-645.0000044
GCAGGGC302.0760235E-645.0000043
CCTCGAT302.0760235E-645.00000414
AGGGCGA302.0760235E-645.0000045
AGGGAAT302.0760235E-645.0000045
AACTTCT302.0760235E-645.00000416
ATCAGAA302.0760235E-645.00000419
GACAAGT302.0760235E-645.00000429
TTCTTAG302.0760235E-645.00000419
GCGTCCC302.0760235E-645.0000047
AAGGACT302.0760235E-645.00000424
CCCCCAA302.0760235E-645.00000411
GGGCGAT302.0760235E-645.0000046
ACAGGGA302.0760235E-645.0000043
CGGTCGG302.0760235E-645.0000041
CCCCAAC302.0760235E-645.00000412
Produced by FastQC (version 0.11.5)