##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043387_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	23589
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33163762770783	33.0	31.0	34.0	31.0	34.0
2	32.63991691042435	34.0	31.0	34.0	31.0	34.0
3	32.75204544491076	34.0	31.0	34.0	31.0	34.0
4	36.21895798889313	37.0	37.0	37.0	35.0	37.0
5	36.23239645597524	37.0	37.0	37.0	35.0	37.0
6	36.333333333333336	37.0	37.0	37.0	35.0	37.0
7	36.267794310907625	37.0	37.0	37.0	35.0	37.0
8	36.204841239560814	37.0	37.0	37.0	35.0	37.0
9	37.80473949722328	39.0	38.0	39.0	35.0	39.0
10	37.58103353257874	39.0	37.0	39.0	35.0	39.0
11	37.24163805163423	39.0	37.0	39.0	35.0	39.0
12	37.25732332867014	39.0	37.0	39.0	35.0	39.0
13	37.2379922845394	39.0	37.0	39.0	35.0	39.0
14	38.56475475857391	40.0	37.0	41.0	35.0	41.0
15	38.61473568188563	40.0	37.0	41.0	35.0	41.0
16	38.483615244393576	40.0	37.0	41.0	34.0	41.0
17	38.426809105939206	40.0	37.0	41.0	34.0	41.0
18	38.28767645936665	40.0	37.0	41.0	34.0	41.0
19	38.12866166433507	40.0	37.0	41.0	34.0	41.0
20	37.93285005723006	40.0	35.0	41.0	34.0	41.0
21	37.838696002373986	40.0	35.0	41.0	34.0	41.0
22	37.7826529314511	40.0	35.0	41.0	34.0	41.0
23	37.7626859977108	40.0	35.0	41.0	34.0	41.0
24	37.63071770740599	39.0	35.0	41.0	34.0	41.0
25	37.592606723472805	39.0	35.0	41.0	34.0	41.0
26	37.55703929797787	39.0	35.0	41.0	34.0	41.0
27	37.501759294586456	39.0	35.0	41.0	34.0	41.0
28	37.42396879901649	39.0	35.0	41.0	34.0	41.0
29	37.36199075840434	39.0	35.0	41.0	33.0	41.0
30	37.248505659417525	39.0	35.0	41.0	33.0	41.0
31	37.13154436389843	39.0	35.0	41.0	33.0	41.0
32	36.918309381491376	39.0	35.0	41.0	33.0	41.0
33	36.53758107592522	39.0	35.0	41.0	31.0	41.0
34	36.23048878714655	39.0	35.0	41.0	31.0	41.0
35	36.09254313451185	39.0	35.0	41.0	30.0	41.0
36	35.996015091780066	39.0	35.0	41.0	30.0	41.0
37	35.930857603120096	38.0	35.0	41.0	30.0	41.0
38	35.77985501716902	38.0	35.0	41.0	30.0	41.0
39	35.70643096358472	38.0	35.0	41.0	29.0	41.0
40	35.58285641612616	38.0	35.0	40.0	29.0	41.0
41	35.51384119716817	38.0	35.0	40.0	29.0	41.0
42	35.396455975242695	37.0	35.0	40.0	29.0	41.0
43	35.27648480223833	37.0	35.0	40.0	29.0	41.0
44	35.105260926703124	37.0	35.0	40.0	27.0	41.0
45	35.04807325448302	36.0	35.0	40.0	27.0	41.0
46	34.91517232608419	36.0	35.0	40.0	27.0	41.0
47	34.73457967696808	36.0	35.0	40.0	26.0	41.0
48	34.67378863029378	36.0	35.0	40.0	26.0	41.0
49	34.61236169400992	35.0	34.0	40.0	26.0	41.0
50	34.5214294798423	35.0	34.0	40.0	26.0	41.0
51	34.14260884310484	35.0	34.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	5.0
19	14.0
20	17.0
21	28.0
22	46.0
23	65.0
24	109.0
25	168.0
26	314.0
27	403.0
28	437.0
29	442.0
30	392.0
31	486.0
32	513.0
33	716.0
34	1671.0
35	2513.0
36	1891.0
37	2361.0
38	3804.0
39	7189.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.780363728856674	17.35130781296367	22.281571919114842	31.58675653906482
2	34.89338250879647	19.356479715121456	23.892492263343083	21.857645512738987
3	28.076645894272755	20.865657721819492	30.734664462249356	20.323031921658398
4	22.964093433379965	23.49824070541354	29.76811225571241	23.76955360549409
5	21.832209928356438	32.209928356437324	27.190639704947223	18.76722201025902
6	81.10560006782822	2.0306074865403367	14.472847513671628	2.3909449319598117
7	80.1475263894188	1.8567976599262368	14.040442579168255	3.9552333714867096
8	72.57620077154606	2.942049260248421	17.50392131925898	6.977828648946543
9	43.49908855822629	15.452117512399846	22.900504472423588	18.148289456950273
10	29.975836194836575	16.45258383144686	31.32816143117555	22.243418542541015
11	28.004578405188855	11.963203187926576	38.10674466912544	21.925473737759123
12	23.048878714655135	13.608037644664886	40.40442579168256	22.938657848997416
13	17.66925261774556	18.88592140404426	40.82411293399466	22.620713044215524
14	18.580694391453644	17.41065750985629	41.866971893679256	22.14167620501081
15	23.087032091228963	14.761117470007207	39.32341345542414	22.828436983339692
16	21.429479842299376	18.18220356946034	35.753953113739456	24.634363474500827
17	22.39603204883632	21.175123998473865	34.30412480393404	22.124719148755776
18	22.612234516088005	26.56746788757472	30.569333163762767	20.250964432574506
19	24.532621136970622	22.637670100470558	29.619738013480863	23.20997074907796
20	24.986222391792783	25.600915681037772	31.641866971893677	17.770994955275764
21	20.632498198312774	18.96646742125567	32.77375047691721	27.62728390351435
22	22.824197719275936	21.73894611895375	32.02764000169571	23.40921616007461
23	26.224087498410277	19.526050277671796	32.01068294544067	22.239179278477256
24	26.46996481410827	19.420068676077832	28.746449616346602	25.363516893467292
25	20.250964432574506	19.148755775997287	32.97299588791386	27.62728390351435
26	20.8953325702658	17.571749544279115	32.78646826910848	28.746449616346602
27	22.94289711306117	20.577387765483913	31.794480478188987	24.68523464326593
28	24.56653524948069	22.62919157234304	27.17368264869219	25.63059052948408
29	23.773792869557845	18.88592140404426	32.15481792360846	25.185467802789436
30	27.54249862223918	20.250964432574506	27.805332994192206	24.40120395099411
31	28.60655390224257	21.145449150027556	26.135062953071348	24.11293399465853
32	26.597142736021027	21.10729577345373	25.087964729322987	27.207596761202257
33	22.120479884692017	22.794522870829624	29.640934333799652	25.444062910678706
34	22.56984187545042	24.16380516342363	28.653185806943917	24.613167154182033
35	24.257068972826318	28.835474161685532	24.659799058883376	22.247657806604774
36	23.922167111789395	26.07571325617873	25.664504641994153	24.33761499003773
37	25.193946330916955	28.32252320997075	25.045572088685404	21.437958370426895
38	25.72385433888677	22.17135105345712	24.617406418245793	27.487388189410318
39	26.1096273686888	19.492136165161728	30.51846199499767	23.87977447115181
40	20.323031921658398	21.204798846920177	31.0059773623299	27.466191869091528
41	21.149688414091315	24.528381872906863	27.025308406460642	27.296621306541187
42	21.616007461104754	18.262749586671752	28.50905082877612	31.612192123447368
43	23.70596464453771	19.424307940141592	29.590063165034554	27.27966425028615
44	24.16380516342363	20.161939887235576	30.145406757386915	25.52884819195388
45	26.580185679765993	21.0182712281148	28.66590359913519	23.735639492984017
46	23.544872610114883	19.9754122684302	30.45487303404129	26.024842087413624
47	22.472338801983977	21.662639365806097	30.929670609182246	24.935351223027684
48	23.49400144134978	20.38662088261478	29.200050871168763	26.919326804866678
49	21.671117893933616	17.478485734876426	34.151511297638734	26.69888507355123
50	20.89957183432956	18.788418330577812	34.24477510704142	26.067234728051208
51	20.00508711687651	16.660307770571027	34.19814320234008	29.136461910212386
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	31.5
2	61.0
3	542.0
4	1023.0
5	610.5
6	198.0
7	183.0
8	168.0
9	154.0
10	140.0
11	140.0
12	140.0
13	128.5
14	117.0
15	119.5
16	122.0
17	101.0
18	80.0
19	74.5
20	69.0
21	63.5
22	58.0
23	57.0
24	56.0
25	54.0
26	63.5
27	75.0
28	75.0
29	75.0
30	95.0
31	115.0
32	125.5
33	136.0
34	165.0
35	194.0
36	208.5
37	223.0
38	270.5
39	318.0
40	347.0
41	376.0
42	453.0
43	530.0
44	644.5
45	759.0
46	953.5
47	1148.0
48	1524.5
49	1901.0
50	2266.0
51	2631.0
52	2110.0
53	1589.0
54	1486.5
55	1384.0
56	1387.5
57	1391.0
58	1434.5
59	1478.0
60	1502.5
61	1527.0
62	1483.5
63	1440.0
64	1384.0
65	1328.0
66	1093.0
67	858.0
68	698.0
69	538.0
70	473.0
71	408.0
72	378.0
73	348.0
74	302.0
75	195.5
76	135.0
77	107.0
78	79.0
79	58.5
80	38.0
81	26.5
82	15.0
83	11.0
84	7.0
85	5.0
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	23589.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.30861842384162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.58804898648648	72.75001059816016
2	7.258234797297297	11.657976175335962
3	1.1665962837837838	2.8106320742719064
4	0.390625	1.2548221628725253
5	0.1900337837837838	0.7630675314765357
6	0.04222972972972973	0.20348467506040951
7	0.07390202702702703	0.41544787824833607
8	0.0316722972972973	0.20348467506040951
9	0.0316722972972973	0.22892025944296068
>10	0.17947635135135137	2.543558438255119
>50	0.026393581081081082	1.3141718597651448
>100	0.01583614864864865	2.874221035228284
>500	0.005278716216216216	2.9802026368222476
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	703	2.9802026368222476	No Hit
CGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT	293	1.2421043706812498	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	253	1.0725338081309086	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	132	0.5595828564161262	No Hit
CGGTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC	83	0.3518589172919581	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	60	0.2543558438255119	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	58	0.2458773156979948	No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	57	0.2416380516342363	Illumina Single End Adapter 1 (95% over 21bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	52	0.22044173131544365	No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGCT	50	0.21196320318792658	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG	50	0.21196320318792658	No Hit
GATCCTATTGTCATCATGGGCCGCCGCCCCGCCCGGTGTTACCGGTATTGT	37	0.15685277035906567	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	37	0.15685277035906567	No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	27	0.11446012972148034	Illumina Single End Adapter 1 (95% over 21bp)
GGGCCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC	26	0.11022086565772182	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC	26	0.11022086565772182	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22468099537920216	0.0
2	0.0	0.0	0.0	0.6613251939463309	0.0
3	0.0	0.0	0.0	1.2463436347450083	0.0
4	0.0	0.0	0.0	2.8826995633558012	0.0
5	0.0	0.0	0.0	4.883632201449828	0.0
6	0.0	0.0	0.0	5.625503412607571	0.0
7	0.0	0.0	0.0	7.787528085124422	0.0
8	0.0	0.0	0.0	9.864767476366103	0.0
9	0.0	0.0	0.0	12.370172538047395	0.0
10	0.0	0.0	0.0	13.45542413836958	0.0
11	0.0	0.0	0.0	14.324473271440079	0.0
12	0.0	0.0	0.0	14.977319937258892	0.0
13	0.0	0.0	0.0	15.371571495188435	0.0
14	0.0	0.0	0.0	15.553859849930053	0.0
15	0.0	0.0	0.0	16.04561448132604	0.0
16	0.0	0.0	0.0	16.494976472084446	0.0
17	0.0	0.0	0.0	17.177497986349568	0.0
18	0.0	0.0	0.0	17.444571622366357	0.0
19	0.0	0.0	0.0	17.821866124040866	0.0
20	0.0	0.0	0.0	18.10165755224893	0.0
21	0.0	0.0	0.0	18.44503794141337	0.0
22	0.0	0.0	0.0	18.775700538386538	0.0
23	0.0	0.0	0.0	19.051252702530842	0.0
24	0.0	0.0	0.0	19.33952265886642	0.0
25	0.0	0.0	0.0	19.551485862054346	0.0
26	0.0	0.0	0.0	19.79736317775234	0.0
27	0.0	0.0	0.0	20.12378651066175	0.0
28	0.0	0.0	0.0	20.488363220144983	0.0
29	0.0	0.0	0.0	20.831743609309424	0.0
30	0.0	0.0	0.0	21.10729577345373	0.0
31	0.0	0.0	0.0	21.365890881343	0.0
32	0.0	0.0	0.0	21.666878629869856	0.0
33	0.0	0.0	0.0	21.85340624867523	0.0
34	0.0	0.0	0.0	22.154393997202085	0.0
35	0.0	0.0	0.0	22.417228369155115	0.0
36	0.0	0.0	0.0	22.66310568485311	0.0
37	0.0	0.0	0.0	23.095510619356478	0.0
38	0.0	0.0	0.0	23.489762177286025	0.0
39	0.0	0.0	0.0	23.867056678960534	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAACTT	30	2.0760235E-6	45.000004	14
TCGATCA	30	2.0760235E-6	45.000004	16
CAACTTC	30	2.0760235E-6	45.000004	15
CTGGGCC	30	2.0760235E-6	45.000004	9
TCGGCGT	30	2.0760235E-6	45.000004	4
GCAGGGC	30	2.0760235E-6	45.000004	3
CCTCGAT	30	2.0760235E-6	45.000004	14
AGGGCGA	30	2.0760235E-6	45.000004	5
AGGGAAT	30	2.0760235E-6	45.000004	5
AACTTCT	30	2.0760235E-6	45.000004	16
ATCAGAA	30	2.0760235E-6	45.000004	19
GACAAGT	30	2.0760235E-6	45.000004	29
TTCTTAG	30	2.0760235E-6	45.000004	19
GCGTCCC	30	2.0760235E-6	45.000004	7
AAGGACT	30	2.0760235E-6	45.000004	24
CCCCCAA	30	2.0760235E-6	45.000004	11
GGGCGAT	30	2.0760235E-6	45.000004	6
ACAGGGA	30	2.0760235E-6	45.000004	3
CGGTCGG	30	2.0760235E-6	45.000004	1
CCCCAAC	30	2.0760235E-6	45.000004	12
>>END_MODULE