FastQCFastQC Report
Thu 26 May 2016
SRR1043386_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043386_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2900872
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2058257.095280315711965No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT146500.5050205593352619TruSeq Adapter, Index 23 (96% over 25bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC142110.48988717875176846No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT130730.4506575953713229No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC127740.4403503498258455TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCTT126020.43442109820771135Illumina Single End Adapter 1 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC117380.404636950544526Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA90550.3121475197802592No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT88350.3045635932919481No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT61090.21059184962314778TruSeq Adapter, Index 23 (96% over 25bp)
CGTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG60350.20804089253162494No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCG57270.19742339544798942No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT51950.17908408230352804No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC39690.136820928327758No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC38990.13440786080874992No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTC33510.11551698937422954No Hit
GGGCCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT31600.10893276228665036TruSeq Adapter, Index 23 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT30740.10596813647758328TruSeq Adapter, Index 23 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT474900.045.4333531
CGGGTAT2000.043.6999975
ATAGCGG2700.043.4444471
TACGGGA8150.042.61353
CGACGGT5750.042.427
CGTTCTG15300.042.392161
CTAGCGG6300.042.3492051
TCACGAC5850.042.06837524
CGGTCTA5800.042.03448530
GCGTCAA554.7293724E-1141.8181822
ATTGCGG4900.041.7755131
CACGACG5800.041.6379325
CCGCACT39200.041.24744833
CGCACTT39150.041.12388234
TCTACGG2350.041.1063841
GTTTTTT529750.041.002742
CCCGCAC40100.040.95261432
CGTTTCT14850.040.7340051
CACTTAC39450.040.6945536
ACTTACT38850.040.67181437
Produced by FastQC (version 0.11.5)