##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043386_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2900872
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.388629005347354	33.0	31.0	34.0	31.0	34.0
2	32.72122796179907	34.0	31.0	34.0	31.0	34.0
3	32.78846153846154	34.0	31.0	34.0	31.0	34.0
4	36.25	37.0	37.0	37.0	35.0	37.0
5	36.184484872135	37.0	37.0	37.0	35.0	37.0
6	36.35951224321514	37.0	37.0	37.0	35.0	37.0
7	36.30042311415326	37.0	37.0	37.0	35.0	37.0
8	36.27973347324529	37.0	37.0	37.0	35.0	37.0
9	37.86239551417643	39.0	38.0	39.0	35.0	39.0
10	37.71295114020887	39.0	37.0	39.0	35.0	39.0
11	37.5736509573673	39.0	37.0	39.0	35.0	39.0
12	37.60815196258228	39.0	37.0	39.0	35.0	39.0
13	37.60877281038253	39.0	37.0	39.0	35.0	39.0
14	39.073775747430425	41.0	39.0	41.0	35.0	41.0
15	39.11732506639383	41.0	39.0	41.0	35.0	41.0
16	39.06181210339512	41.0	38.0	41.0	35.0	41.0
17	39.05463357225	41.0	38.0	41.0	35.0	41.0
18	38.72257962433365	40.0	38.0	41.0	35.0	41.0
19	38.39192525557832	40.0	37.0	41.0	35.0	41.0
20	38.034657165155856	40.0	35.0	41.0	35.0	41.0
21	37.961314735707056	40.0	35.0	41.0	34.0	41.0
22	37.8977900438213	40.0	35.0	41.0	34.0	41.0
23	37.899798060721054	40.0	35.0	41.0	34.0	41.0
24	37.80467390494996	40.0	35.0	41.0	34.0	41.0
25	37.71538489116376	40.0	35.0	41.0	34.0	41.0
26	37.62951278098448	40.0	35.0	41.0	34.0	41.0
27	37.648631859661506	40.0	35.0	41.0	34.0	41.0
28	37.60702781784236	40.0	35.0	41.0	34.0	41.0
29	37.551976784911574	40.0	35.0	41.0	34.0	41.0
30	37.48952039248888	40.0	35.0	41.0	34.0	41.0
31	37.32955573358632	40.0	35.0	41.0	33.0	41.0
32	37.05679292295558	40.0	35.0	41.0	33.0	41.0
33	36.72933000835611	40.0	35.0	41.0	32.0	41.0
34	36.42610635698507	40.0	35.0	41.0	31.0	41.0
35	36.20927983034067	40.0	35.0	41.0	30.0	41.0
36	36.13638140531537	40.0	35.0	41.0	30.0	41.0
37	36.063903543486234	40.0	35.0	41.0	29.0	41.0
38	35.951387031209926	39.0	35.0	41.0	29.0	41.0
39	35.896998902399005	39.0	35.0	41.0	28.0	41.0
40	35.8100853812233	39.0	35.0	41.0	28.0	41.0
41	35.73397757639772	39.0	35.0	41.0	27.0	41.0
42	35.63015155442915	39.0	35.0	41.0	27.0	41.0
43	35.507034436541836	39.0	35.0	41.0	26.0	41.0
44	35.33292196277533	38.0	35.0	41.0	24.0	41.0
45	35.25088249326409	38.0	35.0	41.0	24.0	41.0
46	35.2203864906828	38.0	35.0	41.0	23.0	41.0
47	35.14553313624317	38.0	35.0	41.0	23.0	41.0
48	35.06425447244828	38.0	35.0	41.0	23.0	41.0
49	35.0293270437303	38.0	35.0	40.0	24.0	41.0
50	34.944046135093174	38.0	35.0	40.0	24.0	41.0
51	34.83430120322441	37.0	35.0	40.0	24.0	41.0
52	34.5031269907807	37.0	35.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	2.0
12	3.0
13	7.0
14	19.0
15	30.0
16	90.0
17	187.0
18	574.0
19	1078.0
20	2097.0
21	3359.0
22	5397.0
23	8912.0
24	16324.0
25	31321.0
26	47945.0
27	56190.0
28	54738.0
29	49267.0
30	47565.0
31	51429.0
32	60513.0
33	76929.0
34	146247.0
35	215336.0
36	298330.0
37	237508.0
38	384223.0
39	1098760.0
40	6490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.277133220631587	16.320471913272975	22.582726849030223	34.81966801706521
2	39.36109556023155	18.228863596877076	22.33400853260675	20.07603231028463
3	23.186648704251684	19.319053029571798	38.97810727257183	18.51619099360468
4	21.24661136375545	21.91182513395972	37.16068823443434	19.680875267850496
5	20.217438066898506	28.6197046956915	33.55725450829957	17.60560272911042
6	71.61456968801106	1.7570578777691674	23.194922078602573	3.433450355617208
7	71.37119459252253	1.5881776238317307	21.475680416095575	5.564947367550171
8	62.406924538552545	3.5685131918954025	27.163970006260186	6.860592263291865
9	31.898167171802132	20.752553025435112	30.86623608349489	16.48304371926786
10	21.28208345628487	17.216616245046318	44.354628539280604	17.146671759388212
11	19.95110435758627	14.111653323552368	44.762230115634196	21.175012203227165
12	18.09879925760254	14.857670383250277	47.606581745075275	19.436948614071905
13	15.084016116533236	18.589582718575656	45.82011891596734	20.50628224892377
14	14.36568038851766	19.913046835572203	45.60787239147401	20.11340038443613
15	16.319644575837884	16.56870761619265	46.64263021601781	20.469017591951662
16	15.876570906954873	20.940358623200197	42.42562236458555	20.757448105259385
17	16.193854813311308	20.726698730588595	42.459853450962335	20.619593005137766
18	16.93990634540235	25.257129580346877	39.91013736559214	17.89282670865864
19	17.376016590873363	20.940841236703996	39.15333044684495	22.52981172557769
20	17.65389855188371	25.034506865521816	38.77620246601711	18.53539211657736
21	16.335674238642724	20.07372265994501	39.57775455104534	24.012848550366925
22	15.516127564401325	22.932587166893263	38.038596670242605	23.51268859846281
23	15.618476099600395	20.40972507577032	42.51300988116677	21.45878894346252
24	19.74475261231795	19.589488953666347	39.13485324412797	21.53090518988773
25	16.59180411958887	20.737488589637877	40.519816110466095	22.150891180307163
26	16.835386049436167	19.001217564925305	40.466246011544115	23.697150374094413
27	19.622306671924854	19.616205058341077	41.5801869231045	19.181301346629564
28	18.559936460484984	20.00729435838603	38.43499471882937	22.99777446229961
29	18.496748563880104	18.332280776263136	41.21881282593648	21.952157833920282
30	19.873472528260468	18.11255374246089	39.38726010661622	22.62671362266243
31	19.06147530811425	17.926609653924753	39.16005256350504	23.851862474455956
32	17.814850155401547	17.137915771533525	42.22668218383989	22.820551889225033
33	15.890773532923893	17.133985918716853	41.238806813951115	25.736433734408138
34	16.26021417008403	16.515792492740115	42.91809497282196	24.30589836435389
35	19.51723481766862	17.890068917208342	38.730319710762835	23.862376554360207
36	17.694955172099974	18.120758172025514	38.61328593609094	25.57100071978357
37	19.64295563540894	20.112710936573556	38.19648023077199	22.04785319724552
38	18.96571100000276	18.168881632833163	35.83294954068983	27.03245782647425
39	21.812027555852172	17.59801880262211	38.06862212465769	22.52133151686803
40	17.81340231489014	17.10527041524066	40.14168843023753	24.93963883963167
41	19.426710313312686	20.97410709607318	35.885623357390465	23.713559233223666
42	18.81841046416388	17.683234558436222	38.37332360752215	25.12503136987775
43	21.00144370382423	19.213326199846115	37.48186752121431	22.303362575115347
44	18.921172668080494	19.318053330171068	38.979830892228264	22.780943109520173
45	20.257943128824714	18.884494041791573	36.90931554373995	23.948247285643763
46	21.336032751531263	17.715431773618416	37.091157417493775	23.857378057356545
47	18.771424592329478	18.81437719416782	38.156182003204556	24.258016210298145
48	18.85853632976567	17.285767865662464	35.83508682906381	28.020608975508054
49	18.91369215877157	15.710517389253988	40.442942673789126	24.93284777818532
50	18.71544142588849	15.905665606755488	40.768637844068955	24.610255123287068
51	17.316724074692026	15.15265065125245	40.317945776304505	27.21267949775102
52	15.93000311630434	16.11770529689004	43.17219098257352	24.780100604232107
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	670.0
1	4005.0
2	7340.0
3	128303.0
4	249266.0
5	140335.0
6	31404.0
7	29396.0
8	27388.0
9	26821.0
10	26254.0
11	25598.0
12	24942.0
13	24118.5
14	22054.5
15	20814.0
16	19565.5
17	18317.0
18	17176.5
19	16036.0
20	15080.5
21	14125.0
22	13734.5
23	13344.0
24	13870.0
25	14396.0
26	16255.0
27	18114.0
28	19581.5
29	21049.0
30	22886.5
31	24724.0
32	28817.5
33	32911.0
34	35913.0
35	38915.0
36	42851.0
37	46787.0
38	51380.5
39	59956.0
40	63938.0
41	75324.5
42	86711.0
43	104411.0
44	122111.0
45	155485.5
46	188860.0
47	240443.5
48	292027.0
49	309974.0
50	327921.0
51	299835.0
52	271749.0
53	224212.0
54	176675.0
55	151558.5
56	126442.0
57	111830.0
58	97218.0
59	92649.0
60	88080.0
61	82603.5
62	77127.0
63	77902.5
64	65502.0
65	52326.0
66	44251.0
67	36176.0
68	31350.0
69	26524.0
70	23174.0
71	19824.0
72	19267.0
73	18710.0
74	15131.5
75	11553.0
76	8484.5
77	5416.0
78	4301.0
79	3186.0
80	2534.0
81	1882.0
82	1418.5
83	955.0
84	670.0
85	385.0
86	289.5
87	194.0
88	132.5
89	49.5
90	28.0
91	25.0
92	22.0
93	17.0
94	12.0
95	8.5
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2900872.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.537374398954647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.59109664162376	23.083536193728285
2	10.027338829577396	6.124172001279613
3	3.6982786022317504	3.3880715492398092
4	1.9004705553234236	2.3214152352840256
5	1.165493345511402	1.7795553325685947
6	0.818264457921296	1.4992588865340173
7	0.6511463453809241	1.391900981618582
8	0.5249953995646871	1.2825584859388512
9	0.44840155950812455	1.2323705673397267
>10	4.777440166951714	29.47392690626234
>50	0.232529512903168	4.79282069502586
>100	0.15242362232865359	8.667599757882376
>500	0.006796800658106189	1.3840901628960856
>1k	0.0038515203729268103	2.3074050147582956
>5k	6.796800658106136E-4	1.416780303205912
>10k+	7.929600767790492E-4	9.854537926437585
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	205825	7.095280315711965	No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	14650	0.5050205593352619	TruSeq Adapter, Index 23 (96% over 25bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	14211	0.48988717875176846	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT	13073	0.4506575953713229	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC	12774	0.4403503498258455	TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCTT	12602	0.43442109820771135	Illumina Single End Adapter 1 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	11738	0.404636950544526	Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	9055	0.3121475197802592	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT	8835	0.3045635932919481	No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	6109	0.21059184962314778	TruSeq Adapter, Index 23 (96% over 25bp)
CGTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG	6035	0.20804089253162494	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCG	5727	0.19742339544798942	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT	5195	0.17908408230352804	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3969	0.136820928327758	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3899	0.13440786080874992	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTC	3351	0.11551698937422954	No Hit
GGGCCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT	3160	0.10893276228665036	TruSeq Adapter, Index 23 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	3074	0.10596813647758328	TruSeq Adapter, Index 23 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4443146750356445	0.0
2	0.0	0.0	0.0	1.3443199148394	0.0
3	0.0	0.0	0.0	2.345122432151436	0.0
4	0.0	0.0	0.0	2.997305637753062	0.0
5	0.0	0.0	0.0	5.942247710343648	0.0
6	0.0	0.0	0.0	7.340310086070671	0.0
7	0.0	0.0	0.0	10.472437253350027	0.0
8	0.0	0.0	0.0	13.489150848434539	0.0
9	0.0	0.0	0.0	16.507070977278556	0.0
10	0.0	0.0	0.0	18.04367790098977	0.0
11	0.0	0.0	0.0	19.410198037004047	0.0
12	0.0	0.0	0.0	20.4256857937889	0.0
13	0.0	0.0	0.0	20.93049951876539	0.0
14	0.0	0.0	0.0	21.25633257861774	0.0
15	0.0	0.0	0.0	22.01813799436859	0.0
16	0.0	0.0	0.0	22.900907037608	0.0
17	0.0	0.0	0.0	23.963690917765415	0.0
18	0.0	0.0	0.0	24.55685738633073	0.0
19	3.447239312868682E-5	0.0	0.0	25.218796279187774	0.0
20	3.447239312868682E-5	0.0	0.0	25.896695890063402	0.0
21	6.894478625737364E-5	0.0	0.0	26.596795722113903	0.0
22	2.0683435877212093E-4	0.0	0.0	27.319440499270563	0.0
23	2.0683435877212093E-4	0.0	0.0	27.9233278820989	0.0
24	2.4130675190080775E-4	0.0	0.0	28.431313067243227	0.0
25	2.4130675190080775E-4	0.0	0.0	28.906377116949663	0.0
26	2.4130675190080775E-4	0.0	0.0	29.398953142365468	0.0
27	2.4130675190080775E-4	0.0	0.0	29.909696118960092	0.0
28	3.102515381581814E-4	0.0	0.0	30.4205425127341	0.0
29	3.102515381581814E-4	0.0	0.0	30.97420361877394	0.0
30	3.102515381581814E-4	0.0	0.0	31.498838969799426	0.0
31	3.102515381581814E-4	0.0	0.0	31.98934665162751	0.0
32	3.102515381581814E-4	0.0	0.0	32.4897134378904	0.0
33	3.102515381581814E-4	0.0	0.0	32.9704999048562	0.0
34	3.102515381581814E-4	0.0	0.0	33.501202397072326	0.0
35	3.102515381581814E-4	0.0	0.0	33.96575236687451	0.0
36	3.102515381581814E-4	0.0	0.0	34.45729422049646	0.0
37	3.102515381581814E-4	0.0	0.0	34.960625632568416	0.0
38	3.102515381581814E-4	0.0	0.0	35.465990915834965	0.0
39	3.102515381581814E-4	0.0	0.0	36.01561875187875	0.0
40	3.102515381581814E-4	0.0	0.0	36.521707955401	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	47490	0.0	45.433353	1
CGGGTAT	200	0.0	43.699997	5
ATAGCGG	270	0.0	43.444447	1
TACGGGA	815	0.0	42.6135	3
CGACGGT	575	0.0	42.4	27
CGTTCTG	1530	0.0	42.39216	1
CTAGCGG	630	0.0	42.349205	1
TCACGAC	585	0.0	42.068375	24
CGGTCTA	580	0.0	42.034485	30
GCGTCAA	55	4.7293724E-11	41.81818	22
ATTGCGG	490	0.0	41.775513	1
CACGACG	580	0.0	41.63793	25
CCGCACT	3920	0.0	41.247448	33
CGCACTT	3915	0.0	41.123882	34
TCTACGG	235	0.0	41.106384	1
GTTTTTT	52975	0.0	41.00274	2
CCCGCAC	4010	0.0	40.952614	32
CGTTTCT	1485	0.0	40.734005	1
CACTTAC	3945	0.0	40.69455	36
ACTTACT	3885	0.0	40.671814	37
>>END_MODULE