SRR1043385_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043385_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2841328
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	194742	6.85390775017879	No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	14434	0.5080018920730025	TruSeq Adapter, Index 23 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	13598	0.47857903065045637	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC	13017	0.4581308458579932	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT	12288	0.43247382913905047	No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	12131	0.42694824391974456	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG	11866	0.4176216191865212	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT	8601	0.3027105635111469	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	7752	0.27283016955451816	No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	6333	0.22288873371888074	TruSeq Adapter, Index 23 (95% over 24bp)
CGTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT	6196	0.21806704470585586	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCC	5327	0.1874827545429461	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTC	4943	0.17396794738235077	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3808	0.13402183767590364	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3757	0.1322269023498871	No Hit
GGGCCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC	3311	0.11653001694982065	TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGT	3220	0.11332728921124206	No Hit
TCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	3125	0.1099837822314073	TruSeq Adapter, Index 23 (95% over 24bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	45270	44.388668	1
CGGTCTA	655	42.93893	30
CTTACGG	280	42.589287	1
CACGACG	675	41.666668	25
CGTTCTG	1475	41.64407	1
CGACGGT	675	41.333332	27
TCACGAC	690	41.086956	24
CGCACTT	3685	40.970146	34
TACGGGA	610	40.94262	3
CACTTAC	3760	40.63165	36
ACGGGAT	895	40.474857	4
CCGCACT	3725	40.4698	33
ATTGCGG	495	40.454544	1
ACCCGCA	3825	40.411766	31
CCCGCAC	3820	40.40576	32
GCACTTA	3800	40.263157	35
GTTTTTT	50730	39.89503	2
ACTTACT	3805	39.855453	37
CTAGCGG	625	39.6	1
CGTTTCT	1530	39.558823	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content