FastQCFastQC Report
Thu 26 May 2016
SRR1043385_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043385_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2841328
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1947426.85390775017879No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC144340.5080018920730025TruSeq Adapter, Index 23 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC135980.47857903065045637No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC130170.4581308458579932No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT122880.43247382913905047No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT121310.42694824391974456Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG118660.4176216191865212No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT86010.3027105635111469No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG77520.27283016955451816No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC63330.22288873371888074TruSeq Adapter, Index 23 (95% over 24bp)
CGTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT61960.21806704470585586No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCC53270.1874827545429461No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTC49430.17396794738235077No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC38080.13402183767590364No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC37570.1322269023498871No Hit
GGGCCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC33110.11653001694982065TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGT32200.11332728921124206No Hit
TCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC31250.1099837822314073TruSeq Adapter, Index 23 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT452700.044.3886681
CGGTCTA6550.042.9389330
CTTACGG2800.042.5892871
CACGACG6750.041.66666825
CGTTCTG14750.041.644071
CGACGGT6750.041.33333227
TCACGAC6900.041.08695624
CGCACTT36850.040.97014634
TACGGGA6100.040.942623
CACTTAC37600.040.6316536
ACGGGAT8950.040.4748574
CCGCACT37250.040.469833
ATTGCGG4950.040.4545441
ACCCGCA38250.040.41176631
CCCGCAC38200.040.4057632
GCACTTA38000.040.26315735
GTTTTTT507300.039.895032
ACTTACT38050.039.85545337
CTAGCGG6250.039.61
CGTTTCT15300.039.5588231