##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043385_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2841328
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.336114661876415	33.0	31.0	34.0	31.0	34.0
2	32.651029377812065	34.0	31.0	34.0	31.0	34.0
3	32.70446425051948	34.0	31.0	34.0	31.0	34.0
4	36.22180226992449	37.0	37.0	37.0	35.0	37.0
5	36.20196154755804	37.0	37.0	37.0	35.0	37.0
6	36.37332155949612	37.0	37.0	37.0	35.0	37.0
7	36.326841885202974	37.0	37.0	37.0	35.0	37.0
8	36.23514075108541	37.0	37.0	37.0	35.0	37.0
9	37.82362261590355	39.0	38.0	39.0	35.0	39.0
10	37.67105557682886	39.0	37.0	39.0	35.0	39.0
11	37.57495861090307	39.0	37.0	39.0	35.0	39.0
12	37.61766927295969	39.0	37.0	39.0	35.0	39.0
13	37.63157368667046	39.0	37.0	39.0	35.0	39.0
14	39.09240890175298	41.0	39.0	41.0	35.0	41.0
15	39.143785581953225	41.0	39.0	41.0	35.0	41.0
16	39.117576006712355	41.0	39.0	41.0	35.0	41.0
17	39.085542746208816	41.0	39.0	41.0	35.0	41.0
18	38.77932959517521	40.0	38.0	41.0	35.0	41.0
19	38.47243612845824	40.0	37.0	41.0	35.0	41.0
20	38.13674908352714	40.0	35.0	41.0	35.0	41.0
21	38.06505443933259	40.0	35.0	41.0	35.0	41.0
22	38.01707089079473	40.0	35.0	41.0	35.0	41.0
23	37.986799482495506	40.0	35.0	41.0	35.0	41.0
24	37.89808040465585	40.0	35.0	41.0	34.0	41.0
25	37.873739321894554	40.0	35.0	41.0	34.0	41.0
26	37.82299861191668	40.0	35.0	41.0	34.0	41.0
27	37.81026442564885	40.0	35.0	41.0	34.0	41.0
28	37.713777853172886	40.0	35.0	41.0	34.0	41.0
29	37.69948348096383	40.0	35.0	41.0	34.0	41.0
30	37.58891792851793	40.0	35.0	41.0	34.0	41.0
31	37.415776003333654	40.0	35.0	41.0	33.0	41.0
32	37.1517501675273	40.0	35.0	41.0	33.0	41.0
33	36.80305054537878	40.0	35.0	41.0	32.0	41.0
34	36.467841797919846	40.0	35.0	41.0	31.0	41.0
35	36.25495050201877	40.0	35.0	41.0	30.0	41.0
36	36.16797603092638	40.0	35.0	41.0	30.0	41.0
37	36.050435570972446	40.0	35.0	41.0	29.0	41.0
38	35.94161990449536	39.0	35.0	41.0	29.0	41.0
39	35.845952667203505	39.0	35.0	41.0	28.0	41.0
40	35.73184757268432	39.0	35.0	41.0	27.0	41.0
41	35.61864276141297	39.0	35.0	41.0	26.0	41.0
42	35.5003695455083	39.0	35.0	41.0	26.0	41.0
43	35.37023286294296	38.0	35.0	41.0	25.0	41.0
44	35.256677511360884	38.0	35.0	41.0	24.0	41.0
45	35.19802747166114	38.0	35.0	41.0	24.0	41.0
46	35.11047123035426	38.0	35.0	40.0	23.0	41.0
47	34.97923682165523	38.0	35.0	40.0	23.0	41.0
48	34.92712597771183	38.0	35.0	40.0	23.0	41.0
49	34.87970800977571	38.0	35.0	40.0	24.0	41.0
50	34.82225212998992	37.0	35.0	40.0	24.0	41.0
51	34.4331291565071	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	4.0
12	0.0
13	7.0
14	12.0
15	28.0
16	84.0
17	185.0
18	430.0
19	872.0
20	1689.0
21	2944.0
22	4540.0
23	7249.0
24	13353.0
25	26145.0
26	43966.0
27	55522.0
28	55751.0
29	50032.0
30	47489.0
31	50664.0
32	58394.0
33	74113.0
34	141880.0
35	213528.0
36	283347.0
37	235207.0
38	401189.0
39	1071979.0
40	722.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.59038308847131	16.380192642313734	22.635683032722728	34.39374123649223
2	39.0490291863523	18.30295551939093	22.42613313211287	20.2218821621439
3	23.28523845187884	19.45132698512808	38.636616399092254	18.626818163900825
4	21.44715428841725	22.02762229492688	36.70734248210696	19.817880934548914
5	20.399545564609227	28.7804153550734	33.139538976140734	17.680500104176637
6	71.99425761474916	1.7231027181655902	22.795573055979457	3.4870666111057926
7	71.72990939448033	1.5603267204631075	21.096262029586164	5.6135018554704
8	62.67808574018909	3.5044528474009335	26.844735982610946	6.9727254297990235
9	32.055574013278296	20.698842231519908	30.51615301014174	16.729430745060057
10	21.441769482439195	17.175876913893784	44.09293823170011	17.289415371966914
11	20.064490970419467	14.069195812662247	44.57503674338197	21.291276473536318
12	18.24298356261579	14.784107994571553	47.39938507627419	19.573523366538463
13	15.156469087694205	18.59063789889798	45.59716442452262	20.655728588885196
14	14.405517420023314	19.919382767494636	45.41513686557835	20.259962946903702
15	16.36213770462263	16.592734101800286	46.4843552029192	20.560772990657888
16	15.941418942128468	20.947176813095847	42.25816238040803	20.853241864367646
17	16.30836003446276	20.70890794726973	42.17175912108704	20.810972897180473
18	17.05547546780942	25.417375255514322	39.6474465461221	17.879702730554165
19	17.425584093071972	21.052479685555486	38.85169188492142	22.670244336451123
20	17.638125552558524	25.194416132174812	38.430621174324116	18.736837140942544
21	16.337712506264676	20.17419319416836	39.25453168377604	24.233562615790927
22	15.61590214153382	23.02543036214052	37.61086365248926	23.747803843836405
23	15.72071228664906	20.45772258605835	42.114145216602935	21.707419910689648
24	19.871271461795327	19.557826481138395	38.87242162819639	21.698480428869882
25	16.571230072698402	20.76574756592692	40.33476599674518	22.3282563646295
26	16.96372259732069	18.882473266022085	40.16730908927093	23.986495047386292
27	19.748300794558038	19.57123570386805	41.325746270757904	19.354717230816014
28	18.717726358942016	19.996529791703036	38.07694852547823	23.20879532387672
29	18.627768423779305	18.30485603914789	40.90640010586599	22.16097543120682
30	19.96172212430244	18.03807937696739	39.114984260880824	22.885214237849343
31	19.18011577684801	17.868792339356805	38.846764611477454	24.104327272317732
32	17.899622993191915	17.003527927785882	42.09788521423785	22.998963864784354
33	15.944656864677361	16.99092818569345	41.040844281265656	26.023570668363526
34	16.344857052758428	16.341337571727024	42.880230652708875	24.433574722805673
35	19.56451349509807	17.719847902107748	38.587766002376355	24.12787260041783
36	17.690741793978027	17.99732378662372	38.471517543909044	25.840416875489208
37	19.725107414561077	19.892951464948787	38.154905030323846	22.227036090166287
38	19.122044339829827	17.97891690082947	35.66364038224379	27.23539837709691
39	21.97669540440245	17.352590056480633	37.89981304516761	22.770901493949307
40	17.91876897000276	16.934581294380653	39.93305243182061	25.21359730379597
41	19.602911033150697	20.809529909957597	35.72931389828982	23.858245158601893
42	18.986861073413557	17.50709527375931	38.207697245794925	25.298346407032206
43	21.24767010355721	18.990239775203708	37.28168659162194	22.48040352961714
44	19.059784720384272	19.084949009758816	38.94696423644155	22.90830203341536
45	20.38603075744863	18.631428684051965	36.942338230573874	24.040202327925535
46	21.559918460663464	17.49952838954179	37.00741343484455	23.93313971495019
47	19.000375880574154	18.527885552108028	38.055585275617595	24.41615329170022
48	19.037330431403905	16.983748444389384	35.6656464864317	28.31327463777501
49	19.00231159514143	15.485681343371832	40.358487298896854	25.153519762589887
50	18.913409504288136	15.637195001773819	40.719128520184924	24.730266973753118
51	17.477813191578022	15.047365175720648	40.07224790661269	27.402573726088647
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	546.0
1	3696.5
2	6847.0
3	121534.5
4	236222.0
5	134431.5
6	32641.0
7	30056.5
8	27472.0
9	26615.0
10	25758.0
11	24855.0
12	23952.0
13	23128.0
14	22304.0
15	20869.0
16	19434.0
17	18298.5
18	17163.0
19	15857.0
20	14551.0
21	13820.0
22	13089.0
23	12868.0
24	12647.0
25	13465.5
26	15904.5
27	17525.0
28	19364.0
29	21203.0
30	23604.5
31	26006.0
32	29831.5
33	33657.0
34	37539.0
35	41421.0
36	45567.5
37	49714.0
38	54326.0
39	58938.0
40	66204.0
41	73470.0
42	85305.0
43	97140.0
44	115054.0
45	132968.0
46	178926.0
47	224884.0
48	277208.5
49	329533.0
50	334304.5
51	339076.0
52	277842.0
53	216608.0
54	181294.5
55	145981.0
56	126594.5
57	107208.0
58	100069.5
59	92931.0
60	87706.5
61	82482.0
62	77647.0
63	72812.0
64	70340.5
65	67869.0
66	55388.0
67	42907.0
68	36721.5
69	30536.0
70	26609.5
71	22683.0
72	20598.5
73	18514.0
74	16064.5
75	10253.5
76	6892.0
77	5271.5
78	3651.0
79	2983.0
80	2315.0
81	1637.5
82	960.0
83	726.0
84	492.0
85	352.5
86	213.0
87	175.5
88	138.0
89	85.5
90	33.0
91	32.5
92	32.0
93	19.5
94	7.0
95	4.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2841328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.906225258643044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.53805268208271	22.29151793850968
2	10.489442076850995	6.273992351755887
3	3.8697388246680746	3.4718784294792004
4	1.9248519673373907	2.3026022609893695
5	1.2650561730160097	1.8916527437526847
6	0.8567931621691369	1.5374069584737173
7	0.6759912884458031	1.4151443421598373
8	0.5694215203365989	1.3623398603444254
9	0.4743121989209028	1.2766398717465746
>10	4.925432206216885	29.576820154391132
>50	0.24113755805576062	4.913258320607658
>100	0.15682424001086706	8.83213633214045
>500	0.007482609348708373	1.4871908722084908
>1k	0.004038233616763239	2.4881471591710422
>5k	5.938578848181233E-4	1.215106941827609
>10k+	8.314010387453726E-4	9.664165462442297
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	194742	6.85390775017879	No Hit
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	14434	0.5080018920730025	TruSeq Adapter, Index 23 (95% over 24bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	13598	0.47857903065045637	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC	13017	0.4581308458579932	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTT	12288	0.43247382913905047	No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGCT	12131	0.42694824391974456	Illumina Single End Adapter 1 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG	11866	0.4176216191865212	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCT	8601	0.3027105635111469	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	7752	0.27283016955451816	No Hit
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	6333	0.22288873371888074	TruSeq Adapter, Index 23 (95% over 24bp)
CGTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCT	6196	0.21806704470585586	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCC	5327	0.1874827545429461	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTC	4943	0.17396794738235077	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3808	0.13402183767590364	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3757	0.1322269023498871	No Hit
GGGCCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTC	3311	0.11653001694982065	TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGT	3220	0.11332728921124206	No Hit
TCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC	3125	0.1099837822314073	TruSeq Adapter, Index 23 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.438456946892439	0.0
2	0.0	0.0	0.0	1.357147080520095	0.0
3	0.0	0.0	0.0	2.3779725536791245	0.0
4	0.0	0.0	0.0	3.052938625881982	0.0
5	0.0	0.0	0.0	6.078178936046807	0.0
6	0.0	0.0	0.0	7.505856416436258	0.0
7	0.0	0.0	0.0	10.70650766120631	0.0
8	0.0	0.0	0.0	13.817376944865218	0.0
9	0.0	0.0	0.0	16.95851376539421	0.0
10	0.0	0.0	0.0	18.564276985972757	0.0
11	0.0	0.0	0.0	19.95697082491004	0.0
12	0.0	0.0	0.0	20.994865781071386	0.0
13	0.0	0.0	0.0	21.512229492687926	0.0
14	0.0	0.0	0.0	21.85471019185395	0.0
15	0.0	0.0	0.0	22.63033342155499	0.0
16	0.0	0.0	0.0	23.535262384349853	0.0
17	0.0	0.0	0.0	24.61982565898763	0.0
18	0.0	0.0	0.0	25.225246786009922	0.0
19	0.0	0.0	0.0	25.903098832658532	0.0
20	0.0	0.0	0.0	26.606643090836396	0.0
21	0.0	0.0	0.0	27.33411982002782	0.0
22	0.0	0.0	0.0	28.071732654589685	0.0
23	0.0	0.0	0.0	28.678596768834854	0.0
24	1.05584430942151E-4	0.0	0.0	29.18751372597602	0.0
25	1.05584430942151E-4	0.0	0.0	29.673906004516198	0.0
26	1.05584430942151E-4	0.0	0.0	30.183280494191447	0.0
27	1.05584430942151E-4	0.0	0.0	30.712012129539428	0.0
28	1.4077924125620132E-4	0.0	0.0	31.230326101034446	0.0
29	1.4077924125620132E-4	0.0	0.0	31.79115540338884	0.0
30	1.4077924125620132E-4	0.0	0.0	32.30939898526323	0.0
31	1.4077924125620132E-4	0.0	0.0	32.80314697915904	0.0
32	1.4077924125620132E-4	0.0	0.0	33.30625679259839	0.0
33	1.4077924125620132E-4	0.0	0.0	33.78304088792283	0.0
34	1.4077924125620132E-4	0.0	0.0	34.320113693315236	0.0
35	1.4077924125620132E-4	0.0	0.0	34.789577268094355	0.0
36	1.4077924125620132E-4	0.0	0.0	35.2829029242664	0.0
37	1.4077924125620132E-4	0.0	0.0	35.78358429579408	0.0
38	1.4077924125620132E-4	0.0	0.0	36.296583850931675	0.0
39	1.4077924125620132E-4	0.0	0.0	36.848544061086926	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	45270	0.0	44.388668	1
CGGTCTA	655	0.0	42.93893	30
CTTACGG	280	0.0	42.589287	1
CACGACG	675	0.0	41.666668	25
CGTTCTG	1475	0.0	41.64407	1
CGACGGT	675	0.0	41.333332	27
TCACGAC	690	0.0	41.086956	24
CGCACTT	3685	0.0	40.970146	34
TACGGGA	610	0.0	40.94262	3
CACTTAC	3760	0.0	40.63165	36
ACGGGAT	895	0.0	40.474857	4
CCGCACT	3725	0.0	40.4698	33
ATTGCGG	495	0.0	40.454544	1
ACCCGCA	3825	0.0	40.411766	31
CCCGCAC	3820	0.0	40.40576	32
GCACTTA	3800	0.0	40.263157	35
GTTTTTT	50730	0.0	39.89503	2
ACTTACT	3805	0.0	39.855453	37
CTAGCGG	625	0.0	39.6	1
CGTTTCT	1530	0.0	39.558823	1
>>END_MODULE