FastQCFastQC Report
Thu 26 May 2016
SRR1043384_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043384_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2953388
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120963.79550536536344No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC281110.9518221107419683No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA201790.6832492039650733No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT91980.31143893047577903Illumina Single End Adapter 1 (95% over 21bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC84330.2855364753970694No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCTT83110.28140562635183725Illumina Single End Adapter 1 (95% over 22bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC75840.2567898291724623No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT71000.24040187066514795Illumina Single End Adapter 1 (95% over 21bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC55190.1868701301691481No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC45180.1529768523472026No Hit
CGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC44270.14989564527248028No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA38520.13042647969044366No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29750.10073176975053735No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT245400.045.156481
TACGCGG2700.043.4444471
TACGGGT1200.042.1666683
CACTTAC76200.041.8950136
ATTGCGG6050.041.8181841
TCGGCGT28550.041.73034
GCACTTA76550.041.64336835
CTTACTG75850.041.5425238
ACTTACT76250.041.50557737
CGACGGT9550.041.42408427
CGCACTT77600.041.2577334
ACCCGCA78850.041.2162331
CGGTCTA9450.041.1322830
TACGGGA3700.041.0270233
CCGCACT77950.041.0134733
CCCGCAC79200.041.0050532
TCACGAC10350.040.8888924
ATAACGG1350.040.888891
CTTCTTA29800.040.7516818
CACGACG9800.040.60204325
Produced by FastQC (version 0.11.5)