##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043384_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2953388
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32941184835856	33.0	31.0	34.0	31.0	34.0
2	32.676443799460145	34.0	31.0	34.0	31.0	34.0
3	32.832184596131626	34.0	31.0	34.0	31.0	34.0
4	36.218409839817866	37.0	37.0	37.0	35.0	37.0
5	36.182248996745436	37.0	37.0	37.0	35.0	37.0
6	36.2964585079915	37.0	37.0	37.0	35.0	37.0
7	36.24751742744265	37.0	37.0	37.0	35.0	37.0
8	36.230150931743474	37.0	37.0	37.0	35.0	37.0
9	37.83168991002875	39.0	38.0	39.0	35.0	39.0
10	37.66823526065657	39.0	37.0	39.0	35.0	39.0
11	37.44339958041409	39.0	37.0	39.0	35.0	39.0
12	37.46209979860418	39.0	37.0	39.0	35.0	39.0
13	37.45946248850473	39.0	37.0	39.0	35.0	39.0
14	38.87443674857486	41.0	38.0	41.0	35.0	41.0
15	38.927626847539166	41.0	38.0	41.0	35.0	41.0
16	38.82238703482238	41.0	38.0	41.0	35.0	41.0
17	38.81549630458308	41.0	38.0	41.0	35.0	41.0
18	38.622333740097815	40.0	38.0	41.0	35.0	41.0
19	38.40387277255816	40.0	37.0	41.0	35.0	41.0
20	38.180422619716744	40.0	35.0	41.0	34.0	41.0
21	38.10890915788918	40.0	35.0	41.0	34.0	41.0
22	38.04183195706084	40.0	35.0	41.0	34.0	41.0
23	38.043342764310005	40.0	35.0	41.0	34.0	41.0
24	37.950286586117365	40.0	35.0	41.0	34.0	41.0
25	37.850078282975346	40.0	35.0	41.0	34.0	41.0
26	37.74509309308496	40.0	35.0	41.0	34.0	41.0
27	37.79707644237737	40.0	35.0	41.0	34.0	41.0
28	37.77252599387551	40.0	35.0	41.0	34.0	41.0
29	37.730828120111546	40.0	35.0	41.0	34.0	41.0
30	37.69157591213887	40.0	35.0	41.0	34.0	41.0
31	37.61356922964406	40.0	35.0	41.0	34.0	41.0
32	37.442430185265195	40.0	35.0	41.0	33.0	41.0
33	37.1998362558526	40.0	35.0	41.0	33.0	41.0
34	37.02511522360083	40.0	35.0	41.0	33.0	41.0
35	36.84333924293049	40.0	35.0	41.0	32.0	41.0
36	36.74298737585444	39.0	35.0	41.0	32.0	41.0
37	36.70438222136746	39.0	35.0	41.0	32.0	41.0
38	36.534853192333685	39.0	35.0	41.0	32.0	41.0
39	36.47903120077687	39.0	35.0	41.0	31.0	41.0
40	36.386920716140246	39.0	35.0	41.0	31.0	41.0
41	36.34729842472442	39.0	35.0	41.0	31.0	41.0
42	36.2728273426993	39.0	35.0	41.0	31.0	41.0
43	36.17787740723535	38.0	35.0	41.0	31.0	41.0
44	36.00260175771013	38.0	35.0	41.0	31.0	41.0
45	35.916079770081005	38.0	35.0	41.0	31.0	41.0
46	35.86858685685728	38.0	35.0	40.0	31.0	41.0
47	35.76289705246991	37.0	35.0	40.0	31.0	41.0
48	35.65228002551646	37.0	35.0	40.0	30.0	41.0
49	35.62092620407478	37.0	35.0	40.0	31.0	41.0
50	35.52812938902711	37.0	35.0	40.0	30.0	41.0
51	35.40572285117973	36.0	35.0	40.0	30.0	41.0
52	35.02682614001276	36.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	7.0
13	10.0
14	24.0
15	50.0
16	104.0
17	202.0
18	404.0
19	847.0
20	1572.0
21	2732.0
22	4264.0
23	6835.0
24	11444.0
25	19812.0
26	29888.0
27	36574.0
28	39066.0
29	40424.0
30	42798.0
31	49830.0
32	61322.0
33	81721.0
34	174884.0
35	267182.0
36	274457.0
37	301271.0
38	454779.0
39	1048147.0
40	2736.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.13489050541277	17.769998388291683	23.481912975877194	26.613198130418354
2	34.43167643398023	20.33999596395733	24.380575799725605	20.84775180233684
3	28.264691263051112	20.744954608063686	31.069503905345318	19.92085022353988
4	23.560195951226184	24.106111354146492	29.051143974310182	23.28254872031714
5	21.60285069215423	33.14982657205894	26.424872045257853	18.82245069052898
6	80.20541154768692	2.3764571400709964	14.586603588827476	2.8315277234146006
7	80.10359627654748	2.3652158131610204	13.219055538926819	4.312132371364683
8	70.26259333348683	4.269672660686642	18.386781553930604	7.080952451895925
9	40.611257308555466	18.014971280441312	23.026029766491906	18.34774164451132
10	26.2933959235969	17.310458361718812	33.553430839429154	22.84271487525513
11	22.715098727292183	14.375049942642146	39.36590789967319	23.543943430392485
12	19.898164413209507	16.218796852970215	41.67528953188677	22.207749201933506
13	16.836968254763683	20.874839337059676	40.90966036294588	21.378532045230767
14	16.015234029528123	19.628677302135717	40.535479930168336	23.820608738167827
15	18.95768520763273	14.638306920729685	42.05407484556719	24.349933026070396
16	19.58608215378406	19.233368592274363	35.04876433438478	26.131784919556793
17	20.209603343685288	21.16311842534743	34.615465357074655	24.011812873892627
18	21.429659767020116	24.504501271082564	32.6151186366302	21.450720325267117
19	22.4686698801512	19.829023480829473	31.84248056807978	25.859826070939544
20	23.038185297698778	23.29869966289563	33.07509883564232	20.58801620376327
21	20.754570682890293	18.956872581591043	32.905124555256535	27.383432180262123
22	20.323743443123625	22.080031475715348	31.683070426235904	25.913154654925126
23	20.758769250772332	20.970830788233716	35.548055318163414	22.72234464283054
24	25.005417506944568	18.796412797776654	32.21564521830522	23.982524476973563
25	19.488126856342614	20.754976995911136	32.87641176845034	26.880484379295915
26	18.702046598685985	19.957824708436547	34.972512924140005	26.367615768737462
27	22.934101445526288	20.416924562570173	34.63896379344671	22.010010198456822
28	21.422176835552932	22.680155807499723	31.031005746620494	24.86666161032685
29	21.69477901311985	20.113781189603262	34.64394112795203	23.547498669324856
30	24.93912076571043	23.435627150919554	28.683227533937295	22.94202454943272
31	23.380605596013798	21.481633974269553	29.29310337822189	25.844657051494757
32	23.084403403819614	22.964845797436706	28.778609515580072	25.172141283163608
33	19.547008384946373	24.397234633580144	31.376134798407797	24.679622183065685
34	20.721490031110037	22.267544934834163	29.695420987692778	27.315544046363023
35	24.723978021174325	24.571204325337543	28.81463593676144	21.89018171672669
36	21.763208897713408	24.808863583112007	28.575588442832434	24.852339076342155
37	23.267074966106723	25.89073971994198	27.874393747113483	22.967791566837814
38	21.53533501185757	21.916355047152624	27.943602398330324	28.60470754265948
39	23.995255618293296	20.01768816017401	33.57845294962937	22.408603271903317
40	19.955014376709055	19.496862586290728	35.65058163708933	24.89754139991088
41	21.032353351472953	24.002670830923673	29.672701317944	25.292274499659374
42	20.51697914395264	19.731339058735255	31.809975526412376	27.941706270899726
43	22.692683792309037	21.24874889448999	32.75089490442841	23.30767240877257
44	22.018271896547287	22.310309380277836	31.231047190548615	24.44037153262626
45	23.49085186233573	21.44780841528441	30.516376446305056	24.5449632760748
46	23.93095658274497	20.313551758184158	30.624015537409914	25.131476121660956
47	20.180856697460676	23.753025339034355	32.402853942658396	23.663264020846565
48	20.939747842139266	21.765375900491232	30.047829814436845	27.24704644293266
49	20.93798715238228	18.902358917961337	34.225912748341905	25.933741181314478
50	20.49094125120032	19.35597354631359	34.65433596940192	25.498749233084173
51	20.111444889733416	17.67427781246487	33.37577047106578	28.838506826735937
52	18.423214288132815	19.32221570616526	36.21762531709346	26.03694468860847
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	914.0
1	5739.5
2	10565.0
3	74585.5
4	138606.0
5	78161.0
6	17716.0
7	16422.5
8	15129.0
9	14715.5
10	14302.0
11	14123.5
12	13945.0
13	13144.5
14	11615.5
15	10887.0
16	10504.5
17	10122.0
18	9383.5
19	8645.0
20	8351.0
21	8057.0
22	7908.0
23	7759.0
24	8115.0
25	8471.0
26	9692.5
27	10914.0
28	12014.5
29	13115.0
30	14612.0
31	16109.0
32	19550.5
33	22992.0
34	26990.0
35	30988.0
36	34220.0
37	37452.0
38	41857.0
39	52522.0
40	58782.0
41	74886.5
42	90991.0
43	122110.0
44	153229.0
45	193355.0
46	233481.0
47	256925.0
48	280369.0
49	276288.0
50	272207.0
51	246517.5
52	220828.0
53	190674.0
54	160520.0
55	154258.5
56	147997.0
57	145970.5
58	143944.0
59	140960.5
60	137977.0
61	134433.5
62	130890.0
63	134612.5
64	115802.5
65	93270.0
66	80542.0
67	67814.0
68	58287.0
69	48760.0
70	43041.0
71	37322.0
72	37200.5
73	37079.0
74	29369.5
75	21660.0
76	16500.5
77	11341.0
78	8627.5
79	5914.0
80	4314.5
81	2715.0
82	2166.5
83	1618.0
84	1145.5
85	673.0
86	459.0
87	245.0
88	149.0
89	44.0
90	35.0
91	31.5
92	28.0
93	19.0
94	10.0
95	7.5
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2953388.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.531208628633483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.36605227063731	17.752267258815994
2	11.40075441849203	5.59348570327686
3	4.433186279944936	3.262542525687743
4	2.211949094383929	2.170471388409962
5	1.3338858220811225	1.636091569412415
6	0.8446122469104909	1.243161554355609
7	0.5990077858651531	1.028606947566372
8	0.4729850733854496	0.9282316410758387
9	0.35499302358632356	0.7837567130974958
>10	4.6871166230691585	28.47669461198151
>50	1.0795748219857215	17.507264278107318
>100	0.20000768938282218	8.43434960242452
>500	0.008772943573374083	1.480685214971162
>1k	0.0058486290489160545	2.6471966631390074
>5k	8.355184355594364E-4	1.5763345078981261
>10k+	4.177592177797182E-4	5.478859819780016
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	112096	3.79550536536344	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	28111	0.9518221107419683	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	20179	0.6832492039650733	No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	9198	0.31143893047577903	Illumina Single End Adapter 1 (95% over 21bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	8433	0.2855364753970694	No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCTT	8311	0.28140562635183725	Illumina Single End Adapter 1 (95% over 22bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	7584	0.2567898291724623	No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	7100	0.24040187066514795	Illumina Single End Adapter 1 (95% over 21bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5519	0.1868701301691481	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4518	0.1529768523472026	No Hit
CGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	4427	0.14989564527248028	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA	3852	0.13042647969044366	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2975	0.10073176975053735	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2889901360742307	0.0
2	0.0	0.0	0.0	1.0037624585730016	0.0
3	0.0	0.0	0.0	1.6568767801589226	0.0
4	0.0	0.0	0.0	2.0809321362448823	0.0
5	0.0	0.0	0.0	4.36237974827554	0.0
6	0.0	0.0	0.0	4.996295779626653	0.0
7	0.0	0.0	0.0	6.96447605258774	0.0
8	0.0	0.0	0.0	9.178678859668963	0.0
9	0.0	0.0	0.0	11.087029540310992	0.0
10	0.0	0.0	0.0	12.302887395763781	0.0
11	0.0	0.0	0.0	13.400779037498628	0.0
12	0.0	0.0	0.0	14.205651272369225	0.0
13	0.0	0.0	0.0	14.585046055580912	0.0
14	0.0	0.0	0.0	14.831678059232312	0.0
15	0.0	0.0	0.0	15.63035402053506	0.0
16	0.0	0.0	0.0	16.519434628975265	0.0
17	0.0	0.0	0.0	17.598263418148917	0.0
18	0.0	0.0	0.0	18.193986025540838	0.0
19	3.3859418403541965E-5	0.0	0.0	18.847574378984408	0.0
20	3.3859418403541965E-5	0.0	0.0	19.5807323656763	0.0
21	3.3859418403541965E-5	0.0	0.0	20.297570112697688	0.0
22	6.771883680708393E-5	0.0	0.0	21.0069926470887	0.0
23	1.015782552106259E-4	0.0	0.0	21.613685706043363	0.0
24	2.031565104212518E-4	0.0	0.0	22.102006238259246	0.0
25	2.3701592882479375E-4	0.0	0.0	22.583622605631227	0.0
26	2.3701592882479375E-4	0.0	0.0	23.082574995225823	0.0
27	2.3701592882479375E-4	0.0	0.0	23.628185663380496	0.0
28	2.3701592882479375E-4	0.0	0.0	24.169733201326746	0.0
29	2.3701592882479375E-4	0.0	0.0	24.706371123604484	0.0
30	2.708753472283357E-4	0.0	0.0	25.246530425396188	0.0
31	2.708753472283357E-4	0.0	0.0	25.744805626622714	0.0
32	2.708753472283357E-4	0.0	0.0	26.294987316261867	0.0
33	2.708753472283357E-4	0.0	0.0	26.81848778419903	0.0
34	2.708753472283357E-4	0.0	0.0	27.423928044672763	0.0
35	2.708753472283357E-4	0.0	0.0	27.911605247938976	0.0
36	2.708753472283357E-4	0.0	0.0	28.425320343957516	0.0
37	2.708753472283357E-4	0.0	0.0	28.960705467754323	0.0
38	2.708753472283357E-4	0.0	0.0	29.553922478184376	0.0
39	2.708753472283357E-4	0.0	0.0	30.168403203371856	0.0
40	2.708753472283357E-4	0.0	0.0	30.74641733493872	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	24540	0.0	45.15648	1
TACGCGG	270	0.0	43.444447	1
TACGGGT	120	0.0	42.166668	3
CACTTAC	7620	0.0	41.89501	36
ATTGCGG	605	0.0	41.818184	1
TCGGCGT	2855	0.0	41.7303	4
GCACTTA	7655	0.0	41.643368	35
CTTACTG	7585	0.0	41.54252	38
ACTTACT	7625	0.0	41.505577	37
CGACGGT	955	0.0	41.424084	27
CGCACTT	7760	0.0	41.25773	34
ACCCGCA	7885	0.0	41.21623	31
CGGTCTA	945	0.0	41.13228	30
TACGGGA	370	0.0	41.027023	3
CCGCACT	7795	0.0	41.01347	33
CCCGCAC	7920	0.0	41.00505	32
TCACGAC	1035	0.0	40.88889	24
ATAACGG	135	0.0	40.88889	1
CTTCTTA	2980	0.0	40.75168	18
CACGACG	980	0.0	40.602043	25
>>END_MODULE