FastQCFastQC Report
Thu 26 May 2016
SRR1043383_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043383_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2895708
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060663.6628693224593083No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC266780.9212945504173764No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG164010.5663899813102702No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC88620.30603914483090144No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC80310.2773414999026145No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT77340.26708494088492346Illumina Single End Adapter 1 (95% over 21bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC73120.2525116482739282No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC70900.24484512941221975No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC53110.18340937691231296No Hit
CGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG44320.15305410628419716No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC44010.1519835563530577No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA37760.1303998883865362No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT239150.044.0215341
ACGTAGG2100.043.928571
TACGGGA4100.042.804883
TACGCGG1900.042.631581
ACGGGTA2400.041.2500044
ATTGCGG5300.040.754721
CACTTAC70150.040.73414236
GCACTTA70350.040.58635735
CGCACTT70600.040.53824634
ACTTACT70100.040.34593637
ACCCGCA72050.040.22206531
CTTACTG70150.040.1888838
TACGGGT1400.040.178573
CACGACG6900.040.10869625
TATCGGA451.9301297E-840.041
CCGCACT71600.039.94064333
TCGGCGT23950.039.8329854
AGGGCGC15100.039.7847675
CCCGCAC73300.039.75102232
ACTACGG2100.039.6428571