##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043383_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2895708
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35115488163862	33.0	31.0	34.0	31.0	34.0
2	32.639744062591944	34.0	31.0	34.0	31.0	34.0
3	32.76837892494685	34.0	31.0	34.0	31.0	34.0
4	36.210624137516625	37.0	37.0	37.0	35.0	37.0
5	36.21385236356704	37.0	37.0	37.0	35.0	37.0
6	36.33463146145951	37.0	37.0	37.0	35.0	37.0
7	36.293239511718724	37.0	37.0	37.0	35.0	37.0
8	36.1912830989865	37.0	37.0	37.0	35.0	37.0
9	37.80555739736189	39.0	38.0	39.0	35.0	39.0
10	37.6283237121975	39.0	37.0	39.0	35.0	39.0
11	37.448296927728904	39.0	37.0	39.0	35.0	39.0
12	37.47446980151314	39.0	37.0	39.0	35.0	39.0
13	37.48117213475945	39.0	37.0	39.0	35.0	39.0
14	38.89874082607777	41.0	38.0	41.0	35.0	41.0
15	38.964222566640004	41.0	38.0	41.0	35.0	41.0
16	38.89575226507645	41.0	38.0	41.0	35.0	41.0
17	38.860287363228615	41.0	38.0	41.0	35.0	41.0
18	38.690612105916756	40.0	38.0	41.0	35.0	41.0
19	38.496211289259826	40.0	37.0	41.0	35.0	41.0
20	38.29108667034107	40.0	35.0	41.0	35.0	41.0
21	38.21796258462525	40.0	35.0	41.0	35.0	41.0
22	38.16551047274103	40.0	35.0	41.0	34.0	41.0
23	38.133016174282766	40.0	35.0	41.0	34.0	41.0
24	38.04357759829375	40.0	35.0	41.0	34.0	41.0
25	38.014389227090575	40.0	35.0	41.0	34.0	41.0
26	37.97165874459718	40.0	35.0	41.0	34.0	41.0
27	37.96726120175101	40.0	35.0	41.0	34.0	41.0
28	37.88263802841999	40.0	35.0	41.0	34.0	41.0
29	37.87409952937244	40.0	35.0	41.0	34.0	41.0
30	37.78967043638378	40.0	35.0	41.0	34.0	41.0
31	37.69795089836406	40.0	35.0	41.0	34.0	41.0
32	37.538995299249784	40.0	35.0	41.0	33.0	41.0
33	37.26908859594959	40.0	35.0	41.0	33.0	41.0
34	37.05263790409806	40.0	35.0	41.0	33.0	41.0
35	36.876352518969455	40.0	35.0	41.0	33.0	41.0
36	36.77127873390549	39.0	35.0	41.0	32.0	41.0
37	36.67762978863891	39.0	35.0	41.0	32.0	41.0
38	36.51379179116126	39.0	35.0	41.0	32.0	41.0
39	36.41953297777262	39.0	35.0	41.0	31.0	41.0
40	36.3048463450044	39.0	35.0	41.0	31.0	41.0
41	36.224240151285976	38.0	35.0	41.0	31.0	41.0
42	36.1287039300924	38.0	35.0	41.0	31.0	41.0
43	36.02642497102608	38.0	35.0	40.0	31.0	41.0
44	35.90768095401884	38.0	35.0	40.0	31.0	41.0
45	35.850482852552815	38.0	35.0	40.0	31.0	41.0
46	35.74476950023966	37.0	35.0	40.0	31.0	41.0
47	35.57983816047751	37.0	35.0	40.0	30.0	41.0
48	35.5004461775842	37.0	35.0	40.0	30.0	41.0
49	35.450068515195596	37.0	35.0	40.0	30.0	41.0
50	35.381936300207066	37.0	35.0	40.0	30.0	41.0
51	34.99272233250038	36.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	0.0
11	0.0
12	2.0
13	7.0
14	9.0
15	43.0
16	89.0
17	164.0
18	375.0
19	751.0
20	1451.0
21	2367.0
22	3804.0
23	6082.0
24	9952.0
25	17181.0
26	27841.0
27	35672.0
28	38840.0
29	39627.0
30	41810.0
31	47443.0
32	58222.0
33	77691.0
34	168731.0
35	262669.0
36	262025.0
37	295741.0
38	472896.0
39	1023954.0
40	266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.38192524936906	17.82583050500948	23.505719499341783	26.286524746279667
2	34.192743190957096	20.446709405782627	24.45464114475631	20.905906258503965
3	28.325266221594163	20.819329849556652	30.84496088694026	20.01044304190892
4	23.74700764027312	24.247023525852747	28.662420382165603	23.34354845170853
5	21.74255829662383	33.282568546276075	26.186963602683694	18.787909554416398
6	80.46867985307911	2.3580761596127786	14.327238796176964	2.84600519113115
7	80.32522616230642	2.3797634291855396	12.997788451045478	4.297221957462562
8	70.56916650435748	4.244868612442968	18.15604335796289	7.029921525236661
9	40.7317657719632	18.042495997524615	22.812452084257114	18.41328614625508
10	26.55806455623288	17.27826148216602	33.229697193225284	22.933976768375818
11	22.91719330816505	14.373617781903425	39.23897022766107	23.47021868227045
12	20.071844260540082	16.200079566033594	41.51661700696341	22.211459166462916
13	16.920801406771677	20.88774144354334	40.859195747637536	21.332261402047443
14	16.147346348457788	19.607398259769283	40.399204615935034	23.846050775837895
15	19.08863048346035	14.665843379235751	41.948393967900074	24.29713216940382
16	19.69974182479725	19.09726395064696	34.93784594303017	26.265148281525626
17	20.40485435686195	21.036271613021754	34.48724802362669	24.071626006489605
18	21.577486404015875	24.539525394135044	32.48020863982142	21.402779562027664
19	22.629698850850986	19.863466896524095	31.584054745851446	25.922779506773473
20	23.09152027759705	23.388822353635106	32.75433848993061	20.76531887883723
21	20.82672009746839	19.063179022194227	32.74066307790703	27.369437802430358
22	20.449644784626074	22.1336543601772	31.449787064165307	25.966913791031416
23	20.86760819806417	21.02228539617945	35.232696114387224	22.877410291369156
24	25.018372018173103	18.716562581586267	32.14450490173733	24.120560498503302
25	19.373880239305898	20.805240031108106	32.88698307978567	26.933896649800324
26	18.802724584108617	19.810319272523337	34.834106201315876	26.55284994205217
27	22.81925525640016	20.52475594914957	34.529172140284864	22.126816654165406
28	21.421531452756977	22.68388249091414	30.932020770050016	24.962565286278863
29	21.640683383821848	20.161390582199587	34.47208765524701	23.725838378731556
30	25.024208241991253	23.344170061345963	28.5336781194789	23.097943577183887
31	23.489419513293466	21.517811878822034	29.095060689820933	25.89770791806356
32	23.156547552446586	22.95165810917399	28.613934830445608	25.277859507933808
33	19.58664340465268	24.245193230809186	31.294073849987637	24.8740895145505
34	20.72408543955399	22.37763614287076	29.52825353937621	27.370024878199047
35	24.75042373056952	24.604794406065807	28.603263865002965	22.041517998361712
36	21.76086124705944	24.91031554286551	28.393470612368375	24.935352597706675
37	23.238703626194354	25.970125440824837	27.720819916925326	23.070351016055486
38	21.659918748713615	21.8925388885896	27.805842301779048	28.641700060917746
39	24.0275953238379	20.025776079632337	33.46404402653859	22.482584569991175
40	20.033718869444016	19.53194175655833	35.47426052626853	24.96007884772912
41	21.1932280464743	23.947683951558652	29.497656531666866	25.361431470300182
42	20.67439120242787	19.70305707619691	31.724538523911942	27.89801319746328
43	22.828613934830447	21.05540337630728	32.66268560227758	23.453297086584694
44	21.97745767183708	22.251311250996302	31.257744220066385	24.513486857100233
45	23.55876352173631	21.404989729627435	30.47406713660355	24.562179612032704
46	24.028424136687814	20.29327542694222	30.555567066845136	25.12273336952483
47	20.405199695549413	23.53538409259497	32.34304011316058	23.716376098695033
48	21.055714181126	21.646726810852478	30.021051846387824	27.276507161633702
49	21.082098056848274	18.82044045877554	34.12623095975147	25.971230524624723
50	20.662131679022885	19.27835955835326	34.58777611554756	25.471732647076294
51	20.114251851360702	17.663037847738792	33.22382643553839	28.998883865362114
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	788.0
1	5356.0
2	9924.0
3	70608.0
4	131292.0
5	74824.5
6	18357.0
7	16548.0
8	14739.0
9	14379.0
10	14019.0
11	13680.5
12	13342.0
13	12556.0
14	11770.0
15	11064.0
16	10358.0
17	9883.0
18	9408.0
19	8783.5
20	8159.0
21	7722.0
22	7285.0
23	7411.5
24	7538.0
25	8013.5
26	10008.5
27	11528.0
28	12226.5
29	12925.0
30	15249.0
31	17573.0
32	20889.5
33	24206.0
34	29354.0
35	34502.0
36	36660.5
37	38819.0
38	44958.5
39	51098.0
40	60164.0
41	69230.0
42	88101.0
43	106972.0
44	140892.5
45	174813.0
46	217823.0
47	260833.0
48	289673.0
49	318513.0
50	284390.5
51	250268.0
52	211605.5
53	172943.0
54	161392.5
55	149842.0
56	147252.5
57	144663.0
58	144275.5
59	143888.0
60	138534.5
61	133181.0
62	129927.5
63	126674.0
64	124093.0
65	121512.0
66	99471.5
67	77431.0
68	67675.0
69	57919.0
70	48845.5
71	39772.0
72	38688.5
73	37605.0
74	31766.0
75	19865.0
76	13803.0
77	10484.0
78	7165.0
79	5407.5
80	3650.0
81	2710.0
82	1770.0
83	1260.0
84	750.0
85	526.5
86	303.0
87	201.5
88	100.0
89	66.5
90	33.0
91	27.5
92	22.0
93	14.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2895708.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.32423219855744
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.92507125900241	17.495221342017643
2	11.411033803845916	5.5512927174067235
3	4.548717232055407	3.3193216247428556
4	2.2313379369870727	2.171023283708947
5	1.3431845457362601	1.6335966388001344
6	0.8841720443792273	1.2904083666572133
7	0.6283436043569561	1.0698783012999975
8	0.48863921257731696	0.9508618934440741
9	0.3714372883287069	0.8131434163658998
>10	4.8890761214010245	29.408741306753683
>50	1.0642367535597477	17.07311550634115
>100	0.19807865296589014	8.265652542409002
>500	0.009054373471191828	1.442261842306771
>1k	0.006323689408451435	2.7514703451763793
>5k	8.623212829706503E-4	1.5500749003267942
>10k+	4.3116064148532513E-4	5.2139359722426635
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	106066	3.6628693224593083	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	26678	0.9212945504173764	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	16401	0.5663899813102702	No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	8862	0.30603914483090144	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	8031	0.2773414999026145	No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGCT	7734	0.26708494088492346	Illumina Single End Adapter 1 (95% over 21bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	7312	0.2525116482739282	No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC	7090	0.24484512941221975	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	5311	0.18340937691231296	No Hit
CGCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG	4432	0.15305410628419716	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4401	0.1519835563530577	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	3776	0.1303998883865362	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2756148064652928	0.0
2	0.0	0.0	0.0	0.989878813747795	0.0
3	0.0	0.0	0.0	1.644986303867655	0.0
4	0.0	0.0	0.0	2.074276826254581	0.0
5	0.0	0.0	0.0	4.364044993486912	0.0
6	0.0	0.0	0.0	5.009034060064067	0.0
7	0.0	0.0	0.0	7.00281243827071	0.0
8	0.0	0.0	0.0	9.265264315324611	0.0
9	0.0	0.0	0.0	11.248475329694845	0.0
10	0.0	0.0	0.0	12.490693122372836	0.0
11	0.0	0.0	0.0	13.609659537494803	0.0
12	0.0	0.0	0.0	14.464856263131503	0.0
13	0.0	0.0	0.0	14.8479404691357	0.0
14	0.0	0.0	0.0	15.107773297583872	0.0
15	0.0	0.0	0.0	15.919560950206305	0.0
16	0.0	0.0	0.0	16.835295547755507	0.0
17	0.0	0.0	0.0	17.952086329146447	0.0
18	0.0	0.0	0.0	18.557603183746426	0.0
19	0.0	0.0	0.0	19.230530150139447	0.0
20	0.0	0.0	0.0	19.99379771717314	0.0
21	0.0	0.0	0.0	20.728022300591082	0.0
22	0.0	0.0	0.0	21.439661733848855	0.0
23	0.0	0.0	0.0	22.06323980180322	0.0
24	3.453386874643438E-5	0.0	0.0	22.575169872100364	0.0
25	3.453386874643438E-5	0.0	0.0	23.06586161311845	0.0
26	3.453386874643438E-5	0.0	0.0	23.57005609681639	0.0
27	3.453386874643438E-5	0.0	0.0	24.128088881890026	0.0
28	3.453386874643438E-5	0.0	0.0	24.67562337086474	0.0
29	3.453386874643438E-5	0.0	0.0	25.22246718246453	0.0
30	3.453386874643438E-5	0.0	0.0	25.77131395845161	0.0
31	3.453386874643438E-5	0.0	0.0	26.274610561562145	0.0
32	3.453386874643438E-5	0.0	0.0	26.835785928691706	0.0
33	3.453386874643438E-5	0.0	0.0	27.36038992881879	0.0
34	3.453386874643438E-5	0.0	0.0	27.976992155286375	0.0
35	3.453386874643438E-5	0.0	0.0	28.469548725216768	0.0
36	3.453386874643438E-5	0.0	0.0	28.98044277945152	0.0
37	3.453386874643438E-5	0.0	0.0	29.516581091739912	0.0
38	3.453386874643438E-5	0.0	0.0	30.11729773858414	0.0
39	3.453386874643438E-5	0.0	0.0	30.744363727281893	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	23915	0.0	44.021534	1
ACGTAGG	210	0.0	43.92857	1
TACGGGA	410	0.0	42.80488	3
TACGCGG	190	0.0	42.63158	1
ACGGGTA	240	0.0	41.250004	4
ATTGCGG	530	0.0	40.75472	1
CACTTAC	7015	0.0	40.734142	36
GCACTTA	7035	0.0	40.586357	35
CGCACTT	7060	0.0	40.538246	34
ACTTACT	7010	0.0	40.345936	37
ACCCGCA	7205	0.0	40.222065	31
CTTACTG	7015	0.0	40.18888	38
TACGGGT	140	0.0	40.17857	3
CACGACG	690	0.0	40.108696	25
TATCGGA	45	1.9301297E-8	40.0	41
CCGCACT	7160	0.0	39.940643	33
TCGGCGT	2395	0.0	39.832985	4
AGGGCGC	1510	0.0	39.784767	5
CCCGCAC	7330	0.0	39.751022	32
ACTACGG	210	0.0	39.642857	1
>>END_MODULE