FastQCFastQC Report
Thu 26 May 2016
SRR1043382_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043382_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2603862
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1060204.071644349815774No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC172360.6619398416659562No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT108770.41772567056165033TruSeq Adapter, Index 27 (96% over 26bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA106490.4089694461534444No Hit
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCTT99690.3828543909009003TruSeq Adapter, Index 27 (100% over 26bp)
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT77260.29671311306052317TruSeq Adapter, Index 27 (96% over 26bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC51090.1962085548312468No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC46050.17685269034994944TruSeq Adapter, Index 27 (100% over 24bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC44910.17247457814584644No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCG39010.14981592726496257No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC36220.1391010737128158No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAA33820.1298839953883885No Hit
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT33490.1286166471187797TruSeq Adapter, Index 27 (96% over 26bp)
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC30620.11759455762248537No Hit
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC27530.10572756927978517TruSeq Adapter, Index 27 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG206.3129334E-446.010
CGTACAC206.3129334E-446.046
CGTTTTT249200.045.326241
ATTGCGG4800.043.1251
ACGGGTA1400.042.7142874
ATGACGG2450.042.24491
CTAGCGG6400.042.0468751
TACGGGA5700.041.964913
ACGTAGG2250.041.911111
ACTACGG1600.041.68751
ACATACG3200.041.687516
CCCGCAC49400.041.5769232
ACCCGCA49450.041.39534831
CCGCACT48200.041.3713733
GTCGGCG15700.041.3121033
CGCACTT48350.041.1954534
TAGTAGG5200.041.1346131
CATACGA3250.041.04615417
GCGCGAC19350.041.0077488
GCACTTA48250.040.89948335