FastQCFastQC Report
Thu 26 May 2016
SRR1043381_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043381_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2563143
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1008043.9328277821409108No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC165450.6454965641792129No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC104510.407741589134902TruSeq Adapter, Index 27 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT93170.36349903224283625TruSeq Adapter, Index 27 (100% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG88790.3464106372527791No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC77960.30415782498284333TruSeq Adapter, Index 27 (96% over 25bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC49480.1930442429470381No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG45750.17849179698518577TruSeq Adapter, Index 27 (100% over 23bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC44810.17482442454439726No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC36480.14232526238294158No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC34610.13502953210179847No Hit
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC33620.13116708665884033TruSeq Adapter, Index 27 (96% over 25bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA32680.1274997142180518No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC28890.11271318065359599No Hit
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG27330.10662690298590442TruSeq Adapter, Index 27 (95% over 24bp)
GGGCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC25940.10120387352558949TruSeq Adapter, Index 27 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT235600.044.121391
ACGTAGG2450.043.1632651
ACGGGTA1950.042.692314
TCACGAC4200.040.71428324
TAGTAGG5650.040.6194651
CTAGCGG6400.040.4296881
CGCACTT47150.040.22799734
CACGACG4150.040.12048325
CCCGCAC48000.039.937532
CTTGCGG6650.039.924811
ACCCGCA48250.039.77720331
GCACTTA47700.039.7169835
CATATGC8900.039.69101332
CGGTCTA4200.039.64285330
TATGCAA1250.039.6000029
CCGCACT47850.039.59247633
GTTTTTT270850.039.4757232
TTGTAGG8500.039.4411741
ATAACGG1200.039.3749961
CGACGGT4300.039.24418627
Produced by FastQC (version 0.11.5)