##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043381_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2563143
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26954407147787	33.0	31.0	34.0	31.0	34.0
2	32.555413022215305	34.0	31.0	34.0	31.0	34.0
3	32.661841340885	34.0	31.0	34.0	31.0	34.0
4	36.150176560574266	37.0	37.0	37.0	35.0	37.0
5	36.1371788464397	37.0	37.0	37.0	35.0	37.0
6	36.30551982468399	37.0	37.0	37.0	35.0	37.0
7	36.25037502784667	37.0	37.0	37.0	35.0	37.0
8	36.14829956814739	37.0	37.0	37.0	35.0	37.0
9	37.73657458830818	39.0	38.0	39.0	35.0	39.0
10	37.54858273611734	39.0	37.0	39.0	35.0	39.0
11	37.25651280478694	39.0	37.0	39.0	35.0	39.0
12	37.248876086898	39.0	37.0	39.0	34.0	39.0
13	37.19619584237009	39.0	37.0	39.0	34.0	39.0
14	38.50280963645025	40.0	37.0	41.0	34.0	41.0
15	38.60464398591885	40.0	38.0	41.0	35.0	41.0
16	38.52540143097752	40.0	38.0	41.0	34.0	41.0
17	38.473795648545554	40.0	37.0	41.0	34.0	41.0
18	38.31946754433912	40.0	37.0	41.0	34.0	41.0
19	38.16220827320208	40.0	37.0	41.0	34.0	41.0
20	37.909434627720735	40.0	35.0	41.0	34.0	41.0
21	37.85524763932406	40.0	35.0	41.0	34.0	41.0
22	37.83816002462601	40.0	35.0	41.0	34.0	41.0
23	37.809867416683346	40.0	35.0	41.0	34.0	41.0
24	37.73390208817846	40.0	35.0	41.0	34.0	41.0
25	37.69589211370571	40.0	35.0	41.0	34.0	41.0
26	37.668983353640435	40.0	35.0	41.0	34.0	41.0
27	37.64046172999321	39.0	35.0	41.0	34.0	41.0
28	37.571393012407036	39.0	35.0	41.0	34.0	41.0
29	37.53612459390678	39.0	35.0	41.0	34.0	41.0
30	37.44338610838334	39.0	35.0	41.0	33.0	41.0
31	37.352973283191766	40.0	35.0	41.0	33.0	41.0
32	37.150009968230414	39.0	35.0	41.0	33.0	41.0
33	36.81357770518461	39.0	35.0	41.0	32.0	41.0
34	36.59062135823089	39.0	35.0	41.0	31.0	41.0
35	36.47747160419844	39.0	35.0	41.0	31.0	41.0
36	36.43436047071896	39.0	35.0	41.0	31.0	41.0
37	36.3442820006531	39.0	35.0	41.0	31.0	41.0
38	36.257025066490634	39.0	35.0	41.0	31.0	41.0
39	36.194857641575204	39.0	35.0	41.0	31.0	41.0
40	36.11247441129894	38.0	35.0	41.0	31.0	41.0
41	36.0264792093145	38.0	35.0	41.0	31.0	41.0
42	35.91305830380904	38.0	35.0	41.0	30.0	41.0
43	35.787187839305105	38.0	35.0	40.0	30.0	41.0
44	35.658273065529315	38.0	35.0	40.0	30.0	41.0
45	35.600336383884944	38.0	35.0	40.0	30.0	41.0
46	35.50631119683919	37.0	35.0	40.0	29.0	41.0
47	35.374508952485286	37.0	35.0	40.0	29.0	41.0
48	35.32548788733207	37.0	35.0	40.0	29.0	41.0
49	35.27066105948829	37.0	35.0	40.0	29.0	41.0
50	35.198625671685114	37.0	35.0	40.0	29.0	41.0
51	34.8217766234658	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	5.0
13	8.0
14	14.0
15	48.0
16	85.0
17	238.0
18	435.0
19	862.0
20	1564.0
21	2627.0
22	4054.0
23	6338.0
24	10241.0
25	17458.0
26	28099.0
27	35501.0
28	39054.0
29	40556.0
30	44033.0
31	50211.0
32	59623.0
33	78337.0
34	179649.0
35	247217.0
36	226342.0
37	237065.0
38	380389.0
39	872820.0
40	267.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.04750690851037	18.257272419057383	23.824616886377388	26.870603786054854
2	34.16013074572897	20.146788532672584	24.787809341890014	20.905271379708427
3	27.0840136504284	20.623663993776393	32.55924464612392	19.73307770967129
4	23.296398211102538	23.79258589942114	30.532319109780452	22.37869677969587
5	21.30801909998779	32.051196519273404	27.6952163808262	18.94556799991261
6	78.64886976653273	2.167456127106447	16.012684426893074	3.170989679467747
7	78.73852531832988	2.10120933556965	14.254179341534984	4.906086004565489
8	68.78937304707542	3.7516049631253505	19.967399399877415	7.491622589921826
9	40.07884070455687	17.354864711020806	23.974159849840607	18.592134734581723
10	28.891833190734967	15.97729818429951	34.57766499957279	20.55320362539273
11	26.226121601486923	13.543684453032858	37.369237689820665	22.860956255659556
12	22.44135422799274	15.614540429464919	39.94747854489586	21.99662679764648
13	17.643182608227477	19.378630064729123	41.454651574258634	21.523535752784763
14	17.275743101340815	18.84268649856836	41.32703481623928	22.554535583851546
15	21.102997374707535	15.351191876535955	41.282753244746786	22.263057504009726
16	20.492067746512777	19.24005020398784	36.38954205832449	23.878339991174897
17	20.87421575776303	20.12696911565215	36.815425436661165	22.18338968992366
18	21.422956112866117	25.953799690458162	34.0397316887899	18.58351250788583
19	23.08521217895373	21.186605663437426	31.64638102517105	24.081801132437793
20	21.36263954059528	25.67675701277689	33.86190314001208	19.098700306615747
21	19.394001817300087	20.26129638494614	35.13307685135008	25.211624946403692
22	20.450439167849783	22.333010682587744	32.29644229760103	24.92010785196144
23	20.694085347559618	20.675904543757408	36.06743751714204	22.562572591540935
24	24.957991028982775	18.643282875750593	32.10737754389825	24.291348551368376
25	18.102384455334718	20.44271427696387	34.395466815546385	27.059434452155028
26	16.840769321103036	19.831862677969976	35.14302557446073	28.18434242646626
27	21.023992808828847	21.54979257887679	34.188572389445305	23.237642222849058
28	21.050951897728687	22.813982676737115	30.210682743803215	25.924382681730982
29	21.45143677118288	19.253861372541444	32.42203029639782	26.872671559877855
30	23.906157401284283	21.56219922181478	30.76625065398224	23.765392722918698
31	24.047390254855074	18.68873488525611	29.565420267226607	27.698454592662213
32	22.598544053141005	19.90661465240137	28.70514832765866	28.789692966798963
33	17.73494494844806	20.530419098739323	34.411540830925155	27.323095121887462
34	17.945389703188624	21.89113131807316	32.174638715046335	27.988840263691884
35	19.57136219087269	22.8196007791996	32.25832503297709	25.35071199695062
36	19.557863139122553	21.42935450733728	31.172548702901086	27.840233650639078
37	19.34086393150909	23.507506214050483	35.14158203424467	22.01004782019575
38	18.42690790174407	19.66054956746463	32.8745996614313	29.037942869360002
39	21.0939850020073	17.4586045335746	36.04558934089904	25.401821123519053
40	16.904753265814666	18.027203320298554	37.12754224013253	27.940501173754257
41	18.769690181156495	22.585552191196513	30.512889838764362	28.131867788882637
42	18.4223431934933	17.523446799495776	32.61530862694747	31.43890138006346
43	20.981193792152837	18.519528563174198	33.1115353298665	27.387742314806466
44	20.473730884308836	19.53562481687522	32.74191100535553	27.248733293460415
45	20.987163026019225	20.13184594070639	33.23400216062857	25.64698887264581
46	21.36974019787425	18.939715809847517	32.72131129632642	26.969232695951806
47	18.250522893182318	20.370654309962415	35.30388277204978	26.074940024805482
48	20.19594692921932	18.44095315790028	31.468396417991507	29.89470349488889
49	19.45404528736789	16.54753558424169	36.69260747449518	27.305811653895233
50	19.14961436018201	16.492329924627693	37.451597511336665	26.906458203853628
51	17.76721002300691	15.845194747230257	34.88439778818427	31.50319744157856
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	567.0
1	4257.5
2	7948.0
3	69434.0
4	130920.0
5	74286.0
6	17652.0
7	15738.0
8	13824.0
9	13488.5
10	13153.0
11	12845.5
12	12538.0
13	11790.5
14	11043.0
15	10165.5
16	9288.0
17	8951.0
18	8614.0
19	7735.0
20	6856.0
21	6724.5
22	6593.0
23	6641.0
24	6689.0
25	7029.5
26	8038.0
27	8706.0
28	9746.5
29	10787.0
30	12907.5
31	15028.0
32	17501.0
33	19974.0
34	23668.5
35	27363.0
36	30340.5
37	33318.0
38	36502.5
39	39687.0
40	45867.0
41	52047.0
42	63100.0
43	74153.0
44	88855.5
45	103558.0
46	136516.5
47	169475.0
48	225250.5
49	281026.0
50	296812.5
51	312599.0
52	268177.5
53	223756.0
54	199333.0
55	174910.0
56	158727.0
57	142544.0
58	134077.0
59	125610.0
60	121732.0
61	117854.0
62	111517.0
63	105180.0
64	101498.0
65	97816.0
66	79303.0
67	60790.0
68	49286.0
69	37782.0
70	31427.0
71	25072.0
72	23576.0
73	22080.0
74	18408.0
75	11250.0
76	7764.0
77	5761.0
78	3758.0
79	2623.5
80	1489.0
81	1107.5
82	726.0
83	496.0
84	266.0
85	178.0
86	90.0
87	76.5
88	63.0
89	53.0
90	43.0
91	32.5
92	22.0
93	15.5
94	9.0
95	8.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2563143.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.605624254015247
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.2597901716261	18.959112017262523
2	11.903014485081163	6.333742617603404
3	4.624150011757646	3.690851931210723
4	2.3707485491986686	2.5230098040292623
5	1.382595453568311	1.8392407566474132
6	0.9121108457140906	1.4560367063448698
7	0.6417750369122383	1.1952377841385644
8	0.49263597802867204	1.0485510180352127
9	0.4215425417143208	1.009386222474039
>10	5.227335064868831	32.41006283168145
>50	0.5701049901479589	9.872640522371109
>100	0.1779038249899281	8.647192088993906
>500	0.009627709231682467	1.7765860062454897
>1k	0.00577662553900948	3.1777331481659536
>5k	4.4435581069303685E-4	1.024289593947313
>10k+	4.4435581069303685E-4	5.036326950848976
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	100804	3.9328277821409108	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	16545	0.6454965641792129	No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	10451	0.407741589134902	TruSeq Adapter, Index 27 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGCT	9317	0.36349903224283625	TruSeq Adapter, Index 27 (100% over 25bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	8879	0.3464106372527791	No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	7796	0.30415782498284333	TruSeq Adapter, Index 27 (96% over 25bp)
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4948	0.1930442429470381	No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	4575	0.17849179698518577	TruSeq Adapter, Index 27 (100% over 23bp)
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	4481	0.17482442454439726	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCC	3648	0.14232526238294158	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3461	0.13502953210179847	No Hit
TCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC	3362	0.13116708665884033	TruSeq Adapter, Index 27 (96% over 25bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	3268	0.1274997142180518	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2889	0.11271318065359599	No Hit
GCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG	2733	0.10662690298590442	TruSeq Adapter, Index 27 (95% over 24bp)
GGGCCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTC	2594	0.10120387352558949	TruSeq Adapter, Index 27 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3758276459799551	0.0
2	0.0	0.0	0.0	1.322672983910769	0.0
3	0.0	0.0	0.0	2.135230067148029	0.0
4	0.0	0.0	0.0	2.676557648168674	0.0
5	0.0	0.0	0.0	5.540268334618864	0.0
6	0.0	0.0	0.0	6.334020380446975	0.0
7	0.0	0.0	0.0	8.849525758024425	0.0
8	0.0	0.0	0.0	11.679996004904915	0.0
9	0.0	0.0	0.0	14.146928204942135	0.0
10	0.0	0.0	0.0	15.708136455905894	0.0
11	0.0	0.0	0.0	17.110555283103597	0.0
12	0.0	0.0	0.0	18.12540306959073	0.0
13	0.0	0.0	0.0	18.613319662617343	0.0
14	0.0	0.0	0.0	18.94221274427529	0.0
15	0.0	0.0	0.0	19.915353922898568	0.0
16	0.0	0.0	0.0	20.947953352583138	0.0
17	0.0	0.0	0.0	22.246125167421404	0.0
18	0.0	0.0	0.0	22.955878778515284	0.0
19	0.0	0.0	0.0	23.7457293643	0.0
20	0.0	0.0	0.0	24.6540282770021	0.0
21	0.0	0.0	0.0	25.504624595662435	0.0
22	0.0	0.0	0.0	26.317883941707507	0.0
23	0.0	0.0	0.0	27.061346167576293	0.0
24	1.1704380130176116E-4	0.0	0.0	27.650115502724585	0.0
25	1.1704380130176116E-4	0.0	0.0	28.229716406770905	0.0
26	1.1704380130176116E-4	0.0	0.0	28.810448734229812	0.0
27	1.1704380130176116E-4	0.0	0.0	29.421729493828476	0.0
28	1.1704380130176116E-4	0.0	0.0	30.04268587433475	0.0
29	1.1704380130176116E-4	0.0	0.0	30.646475830650104	0.0
30	1.1704380130176116E-4	0.0	0.0	31.20910538350767	0.0
31	1.1704380130176116E-4	0.0	0.0	31.76849672452922	0.0
32	1.1704380130176116E-4	0.0	0.0	32.421250004389144	0.0
33	1.1704380130176116E-4	0.0	0.0	32.995271820573414	0.0
34	1.1704380130176116E-4	0.0	0.0	33.64759593982856	0.0
35	1.1704380130176116E-4	0.0	0.0	34.1661389941958	0.0
36	1.1704380130176116E-4	0.0	0.0	34.76247716182827	0.0
37	1.1704380130176116E-4	0.0	0.0	35.460214275988506	0.0
38	1.1704380130176116E-4	0.0	0.0	36.27405884104008	0.0
39	1.5605840173568155E-4	0.0	0.0	37.07604296755975	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	23560	0.0	44.12139	1
ACGTAGG	245	0.0	43.163265	1
ACGGGTA	195	0.0	42.69231	4
TCACGAC	420	0.0	40.714283	24
TAGTAGG	565	0.0	40.619465	1
CTAGCGG	640	0.0	40.429688	1
CGCACTT	4715	0.0	40.227997	34
CACGACG	415	0.0	40.120483	25
CCCGCAC	4800	0.0	39.9375	32
CTTGCGG	665	0.0	39.92481	1
ACCCGCA	4825	0.0	39.777203	31
GCACTTA	4770	0.0	39.71698	35
CATATGC	890	0.0	39.691013	32
CGGTCTA	420	0.0	39.642853	30
TATGCAA	125	0.0	39.600002	9
CCGCACT	4785	0.0	39.592476	33
GTTTTTT	27085	0.0	39.475723	2
TTGTAGG	850	0.0	39.441174	1
ATAACGG	120	0.0	39.374996	1
CGACGGT	430	0.0	39.244186	27
>>END_MODULE