FastQCFastQC Report
Thu 26 May 2016
SRR1043380_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043380_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences352939
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204765.801569109676176No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT34260.970705986020247TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCTT31420.8902388231394094TruSeq Adapter, Index 20 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT22860.6477039941746308TruSeq Adapter, Index 23 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC10980.3111019184618305TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCG10890.30855190273673355No Hit
TCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT10650.30175186080314165TruSeq Adapter, Index 23 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC10300.2918351329833201TruSeq Adapter, Index 20 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC8630.24451817452874292TruSeq Adapter, Index 23 (95% over 22bp)
CGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG8160.23120142574212543TruSeq Adapter, Index 20 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT6130.17368440438716037TruSeq Adapter, Index 20 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC6090.17255106406489507TruSeq Adapter, Index 20 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT5660.16036765560054286TruSeq Adapter, Index 20 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC5540.1569676346337469TruSeq Adapter, Index 20 (95% over 23bp)
TGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCTTG5120.14506756124996106TruSeq Adapter, Index 23 (96% over 25bp)
AGAGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT4970.14081753504146607No Hit
ATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC4760.13486749834957315TruSeq Adapter, Index 20 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC4700.1331674878661752TruSeq Adapter, Index 23 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC4080.11560071287106272TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCC4020.11390070238766473No Hit
GTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC3980.11276736206539939TruSeq Adapter, Index 23 (95% over 22bp)
ATAGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT3900.11050068142086876No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCGGACCTTATCGTAT3850.10908400601803711No Hit
CGTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG3680.10426730964840951TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCT3530.10001728343991453No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATG1450.046.0000045
CTTAATC301.857712E-646.010
CTTAAGG405.5915734E-946.01
GAAATTG253.412312E-546.045
AGACTGG405.5915734E-946.01
GGCAGGT301.857712E-646.020
AGCCGAA206.3054817E-446.036
GTCGCAG253.412312E-546.039
TCGCAGT253.412312E-546.040
TCCGCGG206.3054817E-446.01
ATGATCG253.412312E-546.026
TTAATCA301.857712E-646.011
ATTAGAC405.5915734E-946.031
TACTTGG206.3054817E-446.01
GTCTGCA206.3054817E-446.08
AAATAGG301.857712E-646.01
ACGTTTT501.6370905E-1146.028
CAACGCA301.857712E-646.016
GGATGGA206.3054817E-446.08
ACGTTGG253.412312E-546.01
Produced by FastQC (version 0.11.5)