##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043380_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	352939
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.143999387996224	33.0	31.0	34.0	30.0	34.0
2	32.44606291738799	34.0	31.0	34.0	31.0	34.0
3	32.50651812352843	34.0	31.0	34.0	31.0	34.0
4	36.05004547528043	37.0	35.0	37.0	35.0	37.0
5	35.915047642793795	37.0	35.0	37.0	35.0	37.0
6	36.218117011721574	37.0	36.0	37.0	35.0	37.0
7	36.1118181895455	37.0	36.0	37.0	35.0	37.0
8	36.084589688303076	37.0	35.0	37.0	35.0	37.0
9	37.694595950008356	39.0	38.0	39.0	35.0	39.0
10	37.55757510504648	39.0	37.0	39.0	35.0	39.0
11	37.10650282343408	39.0	35.0	39.0	34.0	39.0
12	36.939116957887904	39.0	35.0	39.0	33.0	39.0
13	36.97116498885077	39.0	35.0	39.0	33.0	39.0
14	38.37096212093308	40.0	37.0	41.0	34.0	41.0
15	38.48845267879152	40.0	37.0	41.0	34.0	41.0
16	38.52429456648316	40.0	37.0	41.0	34.0	41.0
17	38.55310691082595	40.0	37.0	41.0	35.0	41.0
18	38.28003989357934	40.0	36.0	41.0	34.0	41.0
19	38.02835334151227	40.0	36.0	41.0	34.0	41.0
20	37.70514168170704	39.0	35.0	41.0	34.0	41.0
21	37.63185706311856	39.0	35.0	41.0	34.0	41.0
22	37.59054964172279	39.0	35.0	41.0	34.0	41.0
23	37.61452828959112	39.0	35.0	41.0	34.0	41.0
24	37.53072060611041	39.0	35.0	41.0	34.0	41.0
25	37.45133011653572	39.0	35.0	41.0	33.0	41.0
26	37.39110157845973	39.0	35.0	41.0	33.0	41.0
27	37.44182139123191	39.0	35.0	41.0	33.0	41.0
28	37.42123143092716	39.0	35.0	41.0	33.0	41.0
29	37.347646477153276	39.0	35.0	41.0	33.0	41.0
30	37.23395827607604	39.0	35.0	41.0	33.0	41.0
31	37.10312263592292	39.0	35.0	41.0	33.0	41.0
32	36.91474164090678	39.0	35.0	41.0	32.0	41.0
33	36.70986487750008	39.0	35.0	41.0	31.0	41.0
34	36.546145367896436	39.0	35.0	41.0	31.0	41.0
35	36.44799809598826	39.0	35.0	41.0	31.0	41.0
36	36.40777301460026	39.0	35.0	41.0	31.0	41.0
37	36.396286610434096	39.0	35.0	41.0	31.0	41.0
38	36.35392801588943	39.0	35.0	41.0	31.0	41.0
39	36.31191225679225	39.0	35.0	41.0	31.0	41.0
40	36.22421438265536	39.0	35.0	41.0	31.0	41.0
41	36.22125919776505	39.0	35.0	41.0	31.0	41.0
42	36.060288038442906	39.0	35.0	41.0	30.0	41.0
43	35.87965342452945	38.0	35.0	41.0	29.0	41.0
44	35.692490770359754	38.0	35.0	41.0	28.0	41.0
45	35.6286752101638	38.0	35.0	41.0	28.0	41.0
46	35.577513394665935	38.0	35.0	41.0	28.0	41.0
47	35.41206554107084	38.0	35.0	40.0	28.0	41.0
48	35.39432876502738	38.0	35.0	40.0	28.0	41.0
49	35.227583236763294	37.0	35.0	40.0	28.0	41.0
50	34.790077038808406	37.0	35.0	39.0	27.0	41.0
51	34.51739252392056	36.0	35.0	39.0	27.0	40.0
52	34.125472107078	36.0	34.0	39.0	26.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	2.0
16	14.0
17	25.0
18	63.0
19	137.0
20	251.0
21	413.0
22	727.0
23	1161.0
24	1989.0
25	3311.0
26	4564.0
27	5090.0
28	4959.0
29	5232.0
30	5862.0
31	7072.0
32	8944.0
33	11632.0
34	20893.0
35	43317.0
36	33731.0
37	26385.0
38	44853.0
39	122234.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.760715024409315	19.276702206330274	24.86350332493717	29.099079444323237
2	31.9950473027917	19.957273069850597	28.022406138171185	20.025273489186514
3	20.448576099552614	22.94504149442255	37.78953303545372	18.816849370571116
4	23.180492946373167	23.664145928899895	35.3358512377493	17.819509886977634
5	19.335919238168636	29.81875054896172	30.976174353075177	19.869155859794468
6	71.20833911809122	1.9660621240497649	22.404438160701993	4.421160597157016
7	70.48101796627746	2.0533293288641947	17.70107582330091	9.764576881557437
8	55.5186590317307	3.90662409084856	29.083212679811528	11.491504197609219
9	32.54386735384868	19.250635378918172	28.6854102266964	19.520087040536747
10	27.241251321049813	18.746298935510104	43.71973627170701	10.292713471733075
11	24.86265331969547	13.259798435423686	40.46138284519421	21.416165399686633
12	16.191466514043505	22.9320080807165	44.21698933810092	16.65953606713908
13	9.867994185964147	28.2082739510227	42.135326501180096	19.788405361833064
14	7.560229954751388	21.495215887164637	52.38100634953916	18.56354780854482
15	7.495629556382265	18.278229382414523	53.067810584831946	21.15833047637127
16	7.117093888745647	24.537951317366456	39.497193566026986	28.847761227860907
17	12.13382482525309	21.126313612267275	43.587702124163094	23.152159438316534
18	14.533672957649907	30.153369279110553	39.85277909213774	15.460178671101804
19	15.811514171004054	21.384715205743767	35.95833840975352	26.84543221349865
20	9.89944437990701	34.206477606611905	35.26331745712432	20.630760556356766
21	12.095007919215501	23.736113039363744	34.67454715970749	29.494331881713272
22	8.968121970085482	29.84255069572929	31.573161367828433	29.616165966356796
23	8.98682208540286	22.840207514613006	44.77232609601093	23.40064430397321
24	14.802557949107353	21.46744904926914	40.83623515678347	22.893757844840042
25	10.21054629836884	26.82786543850354	39.44449324104165	23.51709502208597
26	16.622135836504327	23.139126024610484	34.36826193761528	25.87047620126991
27	23.332927219717856	22.7620070323767	39.03875740567067	14.866308342234777
28	15.709513542000176	25.247705694185115	30.911573954706057	28.131206809108654
29	22.644989644102807	23.156976134686165	27.8699718648265	26.328062356384528
30	27.28800160934326	20.638693938612622	25.277455877644577	26.795848574399546
31	25.478907119927243	23.39611094268415	26.84004884696789	24.284933090420722
32	25.136638342603113	20.432425999960333	26.7717650925514	27.659170564885148
33	21.944868660023403	22.002952351539502	29.866067507416293	26.186111481020802
34	18.312796262243616	21.642266793978564	34.51077948314015	25.534157460637672
35	19.356036028888845	20.20320791978217	33.312838762505706	27.127917288823284
36	17.365040417749242	24.478450950447527	30.931690745426266	27.224817886376968
37	14.150037258563094	21.536299473846757	42.214376988658096	22.099286278932055
38	12.352276172369729	24.131082141673208	37.62519868872525	25.891442997231817
39	22.209786960352922	21.83096795763574	36.85367726434313	19.10556781766821
40	14.273288018609447	18.493564043644938	40.704201009239554	26.52894692850606
41	22.856640949285854	21.774867611683604	31.52017770776253	23.848313731268007
42	22.000119000733836	16.87798741425572	36.292673804821796	24.829219780188645
43	24.28833311138752	23.128075956468397	31.66609527425419	20.91749565788989
44	14.542173010066895	22.910757949674025	35.30865107001493	27.23841797024415
45	19.769988581596255	20.33382539192325	34.60824675085497	25.287939275625533
46	24.081215167493532	16.031665528604094	36.23600678870853	23.651112515193844
47	16.322650656345715	15.0731429510482	40.717517758026176	27.886688634579915
48	17.661692247102188	14.92325869342861	32.657201386075215	34.75784767339399
49	20.261008276217705	13.480799798265423	41.81005782868994	24.44813409682693
50	18.634948248847536	14.451222449205103	38.43242033325872	28.481408968688644
51	16.023448811267667	12.336976078019148	39.68617806476473	31.95339704594845
52	14.404188825831094	11.980823881747272	50.067008746553945	23.5479785458677
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	133.0
1	501.0
2	869.0
3	11274.5
4	21680.0
5	11738.0
6	1796.0
7	1800.0
8	1804.0
9	2065.5
10	2327.0
11	2394.0
12	2461.0
13	2378.5
14	2113.0
15	1930.0
16	1780.0
17	1630.0
18	1503.5
19	1377.0
20	1300.5
21	1224.0
22	1137.0
23	1050.0
24	1084.5
25	1119.0
26	1151.0
27	1183.0
28	1180.5
29	1178.0
30	1224.5
31	1271.0
32	1500.0
33	1729.0
34	2157.5
35	2586.0
36	2980.5
37	3375.0
38	3684.5
39	4665.0
40	5336.0
41	9489.5
42	13643.0
43	19195.5
44	24748.0
45	33771.0
46	42794.0
47	52315.0
48	61836.0
49	61990.5
50	62145.0
51	51979.5
52	41814.0
53	30643.0
54	19472.0
55	14417.0
56	9362.0
57	7169.5
58	4977.0
59	4165.5
60	3354.0
61	2836.0
62	2318.0
63	1994.0
64	1306.0
65	942.0
66	681.5
67	421.0
68	389.0
69	357.0
70	313.0
71	269.0
72	243.5
73	218.0
74	170.0
75	122.0
76	87.5
77	53.0
78	49.5
79	46.0
80	32.0
81	18.0
82	12.5
83	7.0
84	4.0
85	1.0
86	1.5
87	2.0
88	1.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	352939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.861944863052948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.83039890181813	20.73165012595374
2	11.49927555136335	6.637829138570036
3	4.679862096697301	4.052097654047065
4	2.5204721076626484	2.9098290800088877
5	1.5020495805959357	2.167603608836585
6	1.0013644432728246	1.734079520973718
7	0.7105489202427462	1.4355476630983561
8	0.5466529610925578	1.2621974097822424
9	0.43972084588310256	1.1422078928111796
>10	4.6210663437327	28.386173625354406
>50	0.42373099691650745	8.360038778599854
>100	0.209866767340723	10.178341444218592
>500	0.006995558911357433	1.3074819135865696
>1k	0.006995558911357433	3.7888997169365055
>5k	0.0	0.0
>10k+	9.993655587653477E-4	5.906022427222282
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20476	5.801569109676176	No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	3426	0.970705986020247	TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCTT	3142	0.8902388231394094	TruSeq Adapter, Index 20 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	2286	0.6477039941746308	TruSeq Adapter, Index 23 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1098	0.3111019184618305	TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCG	1089	0.30855190273673355	No Hit
TCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	1065	0.30175186080314165	TruSeq Adapter, Index 23 (95% over 23bp)
ACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1030	0.2918351329833201	TruSeq Adapter, Index 20 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	863	0.24451817452874292	TruSeq Adapter, Index 23 (95% over 22bp)
CGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	816	0.23120142574212543	TruSeq Adapter, Index 20 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	613	0.17368440438716037	TruSeq Adapter, Index 20 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	609	0.17255106406489507	TruSeq Adapter, Index 20 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT	566	0.16036765560054286	TruSeq Adapter, Index 20 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	554	0.1569676346337469	TruSeq Adapter, Index 20 (95% over 23bp)
TGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCTTG	512	0.14506756124996106	TruSeq Adapter, Index 23 (96% over 25bp)
AGAGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT	497	0.14081753504146607	No Hit
ATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	476	0.13486749834957315	TruSeq Adapter, Index 20 (95% over 23bp)
GGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	470	0.1331674878661752	TruSeq Adapter, Index 23 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	408	0.11560071287106272	TruSeq Adapter, Index 20 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCC	402	0.11390070238766473	No Hit
GTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	398	0.11276736206539939	TruSeq Adapter, Index 23 (95% over 22bp)
ATAGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT	390	0.11050068142086876	No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCGGACCTTATCGTAT	385	0.10908400601803711	No Hit
CGTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	368	0.10426730964840951	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCT	353	0.10001728343991453	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9228223574045373	0.0
2	0.0	0.0	0.0	3.0784356503531773	0.0
3	0.0	0.0	0.0	5.036281057066518	0.0
4	0.0	0.0	0.0	6.507073460286338	0.0
5	0.0	0.0	0.0	13.206248105196648	0.0
6	0.0	0.0	0.0	14.547273041517089	0.0
7	0.0	0.0	0.0	20.85629528048756	0.0
8	0.0	0.0	0.0	27.85920513176498	0.0
9	0.0	0.0	0.0	33.781474985762415	0.0
10	0.0	0.0	0.0	36.42924131365477	0.0
11	0.0	0.0	0.0	39.19289168949875	0.0
12	0.0	0.0	0.0	41.321021479632456	0.0
13	0.0	0.0	0.0	42.06250938547454	0.0
14	5.666701611326603E-4	0.0	0.0	42.609062755886995	0.0
15	5.666701611326603E-4	0.0	0.0	44.55670809969995	0.0
16	5.666701611326603E-4	0.0	0.0	46.61173743904754	0.0
17	5.666701611326603E-4	0.0	0.0	50.13472583080929	0.0
18	5.666701611326603E-4	0.0	0.0	51.13829868617523	0.0
19	5.666701611326603E-4	0.0	0.0	52.7374418808916	0.0
20	5.666701611326603E-4	0.0	0.0	53.793998396323445	0.0
21	5.666701611326603E-4	0.0	0.0	54.61425345456297	0.0
22	0.001416675402831651	0.0	0.0	55.54727587486789	0.0
23	0.001416675402831651	0.0	0.0	56.1269794497066	0.0
24	0.007650047175290915	0.0	0.0	56.617432474166925	0.0
25	0.007650047175290915	0.0	0.0	57.09881877604912	0.0
26	0.007650047175290915	0.0	0.0	57.62213866985513	0.0
27	0.008500052416989905	0.0	0.0	58.05620801328275	0.0
28	0.009066722578122565	0.0	0.0	58.51011081235001	0.0
29	0.009066722578122565	0.0	0.0	59.15469812063841	0.0
30	0.009066722578122565	0.0	0.0	59.66243458501327	0.0
31	0.009066722578122565	0.0	0.0	60.041820257891594	0.0
32	0.009066722578122565	0.0	0.0	60.41865591504481	0.0
33	0.009066722578122565	0.0	0.0	60.76914140970536	0.0
34	0.009066722578122565	0.0	0.0	61.283677916013815	0.0
35	0.009066722578122565	0.0	0.0	61.63076338970757	0.0
36	0.009633392739255226	0.0	0.0	61.95234870615035	0.0
37	0.009633392739255226	0.0	0.0	62.26486730001502	0.0
38	0.009633392739255226	0.0	0.0	62.59098597774686	0.0
39	0.009633392739255226	0.0	0.0	62.94260481272968	0.0
40	0.009633392739255226	0.0	0.0	63.297340333598726	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGATG	145	0.0	46.000004	5
CTTAATC	30	1.857712E-6	46.0	10
CTTAAGG	40	5.5915734E-9	46.0	1
GAAATTG	25	3.412312E-5	46.0	45
AGACTGG	40	5.5915734E-9	46.0	1
GGCAGGT	30	1.857712E-6	46.0	20
AGCCGAA	20	6.3054817E-4	46.0	36
GTCGCAG	25	3.412312E-5	46.0	39
TCGCAGT	25	3.412312E-5	46.0	40
TCCGCGG	20	6.3054817E-4	46.0	1
ATGATCG	25	3.412312E-5	46.0	26
TTAATCA	30	1.857712E-6	46.0	11
ATTAGAC	40	5.5915734E-9	46.0	31
TACTTGG	20	6.3054817E-4	46.0	1
GTCTGCA	20	6.3054817E-4	46.0	8
AAATAGG	30	1.857712E-6	46.0	1
ACGTTTT	50	1.6370905E-11	46.0	28
CAACGCA	30	1.857712E-6	46.0	16
GGATGGA	20	6.3054817E-4	46.0	8
ACGTTGG	25	3.412312E-5	46.0	1
>>END_MODULE