FastQCFastQC Report
Thu 26 May 2016
SRR1043379_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043379_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences344577
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185995.3976324595083245No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC32810.9521819506235182TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT30180.8758564849075824TruSeq Adapter, Index 20 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC23230.6741599120080563TruSeq Adapter, Index 23 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC11200.3250362038093082TruSeq Adapter, Index 23 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG10950.3177809314028504TruSeq Adapter, Index 20 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG9560.27744161682294527TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCC9280.2693157117277125No Hit
GCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG8620.25016179257466403TruSeq Adapter, Index 23 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT8120.23565124776174845TruSeq Adapter, Index 20 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG6460.18747623898286886TruSeq Adapter, Index 20 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG5900.17122442879240343TruSeq Adapter, Index 20 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC5650.16396915638594567TruSeq Adapter, Index 20 (95% over 23bp)
TGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCTT5420.1572943057720045TruSeq Adapter, Index 23 (95% over 24bp)
GGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG4930.1430739718553473TruSeq Adapter, Index 23 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC4810.13959144110024757No Hit
ATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG4780.13872080841147263TruSeq Adapter, Index 20 (95% over 22bp)
AGAGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC4580.1329165904863064No Hit
TGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG4360.12653195076862356TruSeq Adapter, Index 20 (95% over 22bp)
ATAGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC4060.11782562388087423No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC3630.10534655534176686No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCGGACCTTATCGTA3610.10476613354925024No Hit
GTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG3450.10012275920911727TruSeq Adapter, Index 23 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAA207.026402E-445.0000049
GTGCAAG207.026402E-445.0000041
ATGGGCA207.026402E-445.0000044
AGCAAGG207.026402E-445.0000041
CCTTAAC207.026402E-445.00000433
GTACCGG302.1610613E-645.0000041
GTTCTTC207.026402E-445.00000435
AATTTGG406.7902874E-945.0000041
GATAATC207.026402E-445.0000048
GATGTGG207.026402E-445.00000410
GGTGTCT207.026402E-445.0000047
CGAGTTG207.026402E-445.00000410
ATTAGAG207.026402E-445.00000436
AATCAGG302.1610613E-645.0000041
GGTCGGA207.026402E-445.0000047
CACGGGT600.045.0000043
CACGGGA600.045.0000043
CAACGCA302.1610613E-645.00000416
CATGCGG406.7902874E-945.0000041
ACGTTGG302.1610613E-645.0000041
Produced by FastQC (version 0.11.5)