##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043379_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	344577
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06922400508449	33.0	31.0	34.0	30.0	34.0
2	32.33614837902704	34.0	31.0	34.0	30.0	34.0
3	32.396146579719485	34.0	31.0	34.0	30.0	34.0
4	35.99942248031645	37.0	35.0	37.0	35.0	37.0
5	35.92958613024084	37.0	35.0	37.0	35.0	37.0
6	36.223627810329766	37.0	36.0	37.0	35.0	37.0
7	36.1250837983963	37.0	36.0	37.0	35.0	37.0
8	36.012566131807986	37.0	35.0	37.0	35.0	37.0
9	37.6266814093802	39.0	37.0	39.0	35.0	39.0
10	37.48370320712061	39.0	37.0	39.0	35.0	39.0
11	37.09358430771642	39.0	35.0	39.0	34.0	39.0
12	36.93278135220865	39.0	35.0	39.0	33.0	39.0
13	36.96591763234343	39.0	35.0	39.0	33.0	39.0
14	38.36108039712459	40.0	37.0	41.0	34.0	41.0
15	38.49722123066832	40.0	37.0	41.0	34.0	41.0
16	38.561317209215936	40.0	37.0	41.0	35.0	41.0
17	38.57004965508435	40.0	37.0	41.0	35.0	41.0
18	38.33859775899146	40.0	37.0	41.0	35.0	41.0
19	38.1120997628977	40.0	37.0	41.0	34.0	41.0
20	37.80804870899683	39.0	35.0	41.0	34.0	41.0
21	37.74059789248847	39.0	35.0	41.0	34.0	41.0
22	37.70637332149273	39.0	35.0	41.0	34.0	41.0
23	37.71192795804711	39.0	35.0	41.0	34.0	41.0
24	37.62814117018838	39.0	35.0	41.0	34.0	41.0
25	37.619507396024694	39.0	35.0	41.0	34.0	41.0
26	37.61479437106946	39.0	35.0	41.0	34.0	41.0
27	37.61453318126282	39.0	35.0	41.0	34.0	41.0
28	37.55256444858479	39.0	35.0	41.0	34.0	41.0
29	37.50234055087832	39.0	35.0	41.0	34.0	41.0
30	37.32635085916936	39.0	35.0	41.0	33.0	41.0
31	37.185470881689724	39.0	35.0	41.0	33.0	41.0
32	37.016597161157016	39.0	35.0	41.0	33.0	41.0
33	36.79301578457065	39.0	35.0	41.0	32.0	41.0
34	36.58018381958169	39.0	35.0	41.0	31.0	41.0
35	36.47296830606802	39.0	35.0	41.0	31.0	41.0
36	36.41008250695781	39.0	35.0	41.0	31.0	41.0
37	36.369780919794415	39.0	35.0	41.0	31.0	41.0
38	36.30784701242393	39.0	35.0	41.0	31.0	41.0
39	36.23742443633789	39.0	35.0	41.0	31.0	41.0
40	36.11940437115652	39.0	35.0	41.0	30.0	41.0
41	36.07607008012723	39.0	35.0	41.0	30.0	41.0
42	35.88289119703288	38.0	35.0	41.0	29.0	41.0
43	35.69217330233881	38.0	35.0	40.0	28.0	41.0
44	35.557158487072556	38.0	35.0	40.0	28.0	41.0
45	35.51700780957522	38.0	35.0	40.0	28.0	41.0
46	35.423823992895635	38.0	35.0	40.0	28.0	41.0
47	35.22068216973275	38.0	35.0	40.0	27.0	41.0
48	35.21482861595521	37.0	35.0	40.0	27.0	41.0
49	35.07146443320361	37.0	35.0	40.0	27.0	41.0
50	34.674058338194364	37.0	35.0	39.0	26.0	41.0
51	34.04485209401672	36.0	34.0	39.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	3.0
16	9.0
17	21.0
18	45.0
19	124.0
20	196.0
21	399.0
22	612.0
23	969.0
24	1702.0
25	2856.0
26	4235.0
27	5053.0
28	5064.0
29	5224.0
30	5976.0
31	7120.0
32	8493.0
33	11312.0
34	20840.0
35	42052.0
36	31304.0
37	26075.0
38	46168.0
39	118712.0
40	11.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.068550715805173	19.496658221529586	24.93433978472156	28.500451277943682
2	31.72382370268474	19.877995339213005	28.11563162950516	20.282549328597092
3	20.771554688792346	22.939139872945667	37.24682726937665	19.042478168885328
4	23.445557886916422	23.917440804232434	34.619548025550166	18.017453283300974
5	19.552088502714923	30.023187850611038	30.44892723542198	19.975796411252055
6	71.89481596276013	1.922066765918793	21.71386946894308	4.469247802377988
7	71.23255469749867	1.9644375567725068	17.075718924942755	9.727288820786065
8	56.022021202808084	3.8409412119787447	28.389590715573004	11.747446869640168
9	32.671362278968125	19.383475971988844	28.292079854430213	19.653081894612814
10	27.644038923085407	18.839330541504513	43.131143401910165	10.385487133499915
11	25.12994192879966	13.10418280964777	40.40432182066708	21.36155344088549
12	16.5080664118615	22.862524196333474	43.719981310418284	16.909428081386743
13	10.011405288222953	28.171061910690497	41.97233129315073	19.845201507935815
14	7.642703952962618	21.37345208763208	52.05425782916445	18.929586130240846
15	7.554770051396349	18.24323736058994	52.8224460715602	21.37954651645351
16	7.141799946020773	24.326928378852912	39.18427521279714	29.346996462329173
17	12.105567115622923	21.225734741436604	43.253902611027435	23.41479553191304
18	14.698601473690932	30.034215864668855	39.67734352554001	15.589839136100203
19	15.951151701941802	21.581243089353034	35.717415846095356	26.75018936260981
20	9.869492159952637	34.238791329659264	34.943133174878184	20.948583335509916
21	12.108179013689249	23.904961735693327	34.18278062668141	29.804078623936014
22	9.028460982596052	29.90333075045636	31.108286391720863	29.959921875226726
23	8.998859471177704	23.042745162909885	44.10857370050816	23.84982166540425
24	14.81729773026058	21.46283704367964	40.64258496649516	23.077280259564624
25	10.236318732823142	26.812584705305348	39.047585880659476	23.903510681212037
26	16.48426911836832	23.144028765704036	34.20483665479704	26.166865461130605
27	23.610397675991145	22.56215591870612	38.818609483511665	15.008836921791065
28	15.879759821462255	25.427408097464426	30.55311294717871	28.1397191338946
29	22.73802372183866	23.233994143544113	27.24296746445642	26.785014670160805
30	27.642297657707854	20.693197746802603	24.638324670538083	27.02617992495146
31	25.72400363344042	23.138224547778872	26.264666533169656	24.873105285611054
32	25.424505988501846	20.441875110642904	26.273663070953663	27.85995582990159
33	22.47538286072489	21.642477588463535	29.377468606436295	26.504670944375274
34	18.706413951018206	21.69529597158255	33.97673089033801	25.621559187061237
35	19.60577751852271	20.10348920560571	32.93313250739312	27.357600768478452
36	17.585619469668607	24.465939398160643	30.34009814932511	27.608342982845635
37	14.304495076572147	21.505498045429615	41.90558278701132	22.28442409098692
38	12.38474999782342	23.99202500457082	37.51875487917069	26.10447011843507
39	22.31895918764166	21.747824143805303	36.630129114827746	19.30308755372529
40	14.302753811194597	18.450738151414633	40.696564193199194	26.549943844191574
41	22.972514126015376	21.626515989169327	31.359318816984306	24.041651067830994
42	22.433012069871175	16.681032106031456	35.84830096030785	25.037654863789516
43	24.58202375666397	22.922597851858946	31.513420802897468	20.98195758857962
44	14.748227536951102	22.91853489931133	35.05486436993763	27.278373193799933
45	19.764232667879746	20.209996604532513	34.582691241725364	25.443079485862373
46	24.401802790087558	15.818815533248012	36.179431592938585	23.599950083725844
47	16.58439187757744	14.967917185418644	40.628074421682236	27.819616515321684
48	17.766130647141278	14.857056623047967	32.34110227902617	35.035710450784585
49	20.553896516598613	13.27947019098779	41.37130452699977	24.795328765413828
50	18.935100137269753	14.251676693453133	38.17492171561073	28.638301453666383
51	16.308111104339524	12.175507941621175	39.125942822649215	32.390438131390084
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	129.0
1	449.0
2	769.0
3	10268.0
4	19767.0
5	10803.5
6	1840.0
7	1862.5
8	1885.0
9	2039.5
10	2194.0
11	2279.5
12	2365.0
13	2254.0
14	2143.0
15	1934.5
16	1726.0
17	1539.0
18	1352.0
19	1271.5
20	1191.0
21	1104.0
22	1017.0
23	1000.5
24	984.0
25	929.5
26	946.0
27	1017.0
28	1080.5
29	1144.0
30	1207.0
31	1270.0
32	1642.5
33	2015.0
34	2450.0
35	2885.0
36	3247.0
37	3609.0
38	4016.0
39	4423.0
40	6318.0
41	8213.0
42	12831.5
43	17450.0
44	22371.5
45	27293.0
46	36951.5
47	46610.0
48	56614.5
49	66619.0
50	63969.5
51	61320.0
52	45957.0
53	30594.0
54	22338.0
55	14082.0
56	10258.5
57	6435.0
58	5209.5
59	3984.0
60	3115.5
61	2247.0
62	2125.5
63	2004.0
64	1634.5
65	1265.0
66	977.5
67	690.0
68	548.5
69	407.0
70	345.0
71	283.0
72	238.5
73	194.0
74	155.0
75	110.0
76	104.0
77	65.5
78	27.0
79	21.5
80	16.0
81	16.5
82	17.0
83	10.5
84	4.0
85	2.5
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	344577.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.361060718665616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.64778533964534	21.33016036433912
2	10.962645689938803	6.437498114790223
3	4.525369378682967	3.9860893530570207
4	2.3654501101801992	2.778084972479004
5	1.4526560782439457	2.132576165832958
6	1.0317555244488847	1.8176061960097432
7	0.7398246910464057	1.5205426372487083
8	0.5598673335308824	1.315063901935812
9	0.4558919715806648	1.2046924672858872
>10	4.626903606301244	28.750291014442585
>50	0.4139019215662722	8.305156914446389
>100	0.20395167149642396	10.04937783077341
>500	0.007998104764565646	1.732185733296156
>1k	0.004998815477853529	3.181104353792653
>5k	0.0	0.0
>10k+	9.997630955707057E-4	5.459569980270327
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18599	5.3976324595083245	No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	3281	0.9521819506235182	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	3018	0.8758564849075824	TruSeq Adapter, Index 20 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	2323	0.6741599120080563	TruSeq Adapter, Index 23 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1120	0.3250362038093082	TruSeq Adapter, Index 23 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	1095	0.3177809314028504	TruSeq Adapter, Index 20 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	956	0.27744161682294527	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCC	928	0.2693157117277125	No Hit
GCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	862	0.25016179257466403	TruSeq Adapter, Index 23 (95% over 21bp)
CGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT	812	0.23565124776174845	TruSeq Adapter, Index 20 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	646	0.18747623898286886	TruSeq Adapter, Index 20 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	590	0.17122442879240343	TruSeq Adapter, Index 20 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	565	0.16396915638594567	TruSeq Adapter, Index 20 (95% over 23bp)
TGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCTT	542	0.1572943057720045	TruSeq Adapter, Index 23 (95% over 24bp)
GGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	493	0.1430739718553473	TruSeq Adapter, Index 23 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	481	0.13959144110024757	No Hit
ATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	478	0.13872080841147263	TruSeq Adapter, Index 20 (95% over 22bp)
AGAGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	458	0.1329165904863064	No Hit
TGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	436	0.12653195076862356	TruSeq Adapter, Index 20 (95% over 22bp)
ATAGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	406	0.11782562388087423	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC	363	0.10534655534176686	No Hit
GCAGAGAATATCAACTGTCTCTTATACACATCTGACGCGGACCTTATCGTA	361	0.10476613354925024	No Hit
GTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG	345	0.10012275920911727	TruSeq Adapter, Index 23 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9083601052885132	0.0
2	0.0	0.0	0.0	3.0855222490183616	0.0
3	0.0	0.0	0.0	5.113806202967696	0.0
4	0.0	0.0	0.0	6.597074093743924	0.0
5	0.0	0.0	0.0	13.260606482731001	0.0
6	0.0	0.0	0.0	14.63852781816546	0.0
7	0.0	0.0	0.0	20.89054115625825	0.0
8	0.0	0.0	0.0	28.126949854459234	0.0
9	0.0	0.0	0.0	34.21847656692118	0.0
10	0.0	0.0	0.0	36.996665476801994	0.0
11	0.0	0.0	0.0	39.857564492116424	0.0
12	0.0	0.0	0.0	42.04749591528164	0.0
13	0.0	0.0	0.0	42.76925041427605	0.0
14	8.706326887749328E-4	0.0	0.0	43.326165124195754	0.0
15	8.706326887749328E-4	0.0	0.0	45.2659347547863	0.0
16	8.706326887749328E-4	0.0	0.0	47.34906856812846	0.0
17	8.706326887749328E-4	0.0	0.0	51.00079227574678	0.0
18	8.706326887749328E-4	0.0	0.0	52.050775298409356	0.0
19	8.706326887749328E-4	0.0	0.0	53.660575139954204	0.0
20	8.706326887749328E-4	0.0	0.0	54.732033768939885	0.0
21	8.706326887749328E-4	0.0	0.0	55.592218865449524	0.0
22	0.0017412653775498656	0.0	0.0	56.50928529762579	0.0
23	0.0017412653775498656	0.0	0.0	57.08506371580227	0.0
24	0.005223796132649596	0.0	0.0	57.596415315009416	0.0
25	0.005223796132649596	0.0	0.0	58.10138227449888	0.0
26	0.005223796132649596	0.0	0.0	58.61853809163119	0.0
27	0.00638463971768284	0.0	0.0	59.068074769935315	0.0
28	0.006674850613941151	0.0	0.0	59.52225482257957	0.0
29	0.006674850613941151	0.0	0.0	60.155785209111464	0.0
30	0.006674850613941151	0.0	0.0	60.683969040301584	0.0
31	0.006674850613941151	0.0	0.0	61.05108582406835	0.0
32	0.006674850613941151	0.0	0.0	61.41733197514634	0.0
33	0.006674850613941151	0.0	0.0	61.7740011666478	0.0
34	0.006674850613941151	0.0	0.0	62.262135894154284	0.0
35	0.006674850613941151	0.0	0.0	62.61067918056051	0.0
36	0.006965061510199462	0.0	0.0	62.95544972531539	0.0
37	0.006965061510199462	0.0	0.0	63.280195718228434	0.0
38	0.006965061510199462	0.0	0.0	63.61132635085917	0.0
39	0.006965061510199462	0.0	0.0	63.984827774343614	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCAAA	20	7.026402E-4	45.000004	9
GTGCAAG	20	7.026402E-4	45.000004	1
ATGGGCA	20	7.026402E-4	45.000004	4
AGCAAGG	20	7.026402E-4	45.000004	1
CCTTAAC	20	7.026402E-4	45.000004	33
GTACCGG	30	2.1610613E-6	45.000004	1
GTTCTTC	20	7.026402E-4	45.000004	35
AATTTGG	40	6.7902874E-9	45.000004	1
GATAATC	20	7.026402E-4	45.000004	8
GATGTGG	20	7.026402E-4	45.000004	10
GGTGTCT	20	7.026402E-4	45.000004	7
CGAGTTG	20	7.026402E-4	45.000004	10
ATTAGAG	20	7.026402E-4	45.000004	36
AATCAGG	30	2.1610613E-6	45.000004	1
GGTCGGA	20	7.026402E-4	45.000004	7
CACGGGT	60	0.0	45.000004	3
CACGGGA	60	0.0	45.000004	3
CAACGCA	30	2.1610613E-6	45.000004	16
CATGCGG	40	6.7902874E-9	45.000004	1
ACGTTGG	30	2.1610613E-6	45.000004	1
>>END_MODULE