##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043378_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2915973
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36075608381834	33.0	31.0	34.0	31.0	34.0
2	32.68083757977183	34.0	31.0	34.0	31.0	34.0
3	32.74877510868585	34.0	31.0	34.0	31.0	34.0
4	36.20835995395019	37.0	37.0	37.0	35.0	37.0
5	36.146287362743074	37.0	37.0	37.0	35.0	37.0
6	36.32314496739167	37.0	37.0	37.0	35.0	37.0
7	36.258215010907165	37.0	37.0	37.0	35.0	37.0
8	36.24215416260713	37.0	37.0	37.0	35.0	37.0
9	37.88416936645161	39.0	38.0	39.0	35.0	39.0
10	37.726210427874335	39.0	38.0	39.0	35.0	39.0
11	37.43339770292798	39.0	37.0	39.0	35.0	39.0
12	37.432514635766516	39.0	37.0	39.0	35.0	39.0
13	37.41784954798964	39.0	37.0	39.0	35.0	39.0
14	38.693678919523606	41.0	38.0	41.0	35.0	41.0
15	38.78578711119753	41.0	38.0	41.0	35.0	41.0
16	38.70609947348621	41.0	38.0	41.0	34.0	41.0
17	38.635643059795136	41.0	38.0	41.0	34.0	41.0
18	38.36564673266865	40.0	38.0	41.0	34.0	41.0
19	38.06568476457087	40.0	37.0	41.0	34.0	41.0
20	37.636349170585596	39.0	35.0	41.0	34.0	41.0
21	37.60499942900706	39.0	35.0	41.0	34.0	41.0
22	37.58748383472687	39.0	35.0	41.0	34.0	41.0
23	37.607875312974436	39.0	35.0	41.0	34.0	41.0
24	37.53269971978479	39.0	35.0	41.0	34.0	41.0
25	37.44649556082995	39.0	35.0	41.0	34.0	41.0
26	37.36576607533746	39.0	35.0	41.0	34.0	41.0
27	37.39114491114972	39.0	35.0	41.0	34.0	41.0
28	37.38360574669244	39.0	35.0	41.0	34.0	41.0
29	37.28995364497545	39.0	35.0	41.0	33.0	41.0
30	37.2261334381354	39.0	35.0	41.0	33.0	41.0
31	37.070789064233445	39.0	35.0	41.0	33.0	41.0
32	36.76260651247457	39.0	35.0	41.0	32.0	41.0
33	36.21445877585286	39.0	35.0	41.0	30.0	41.0
34	35.95130750524782	39.0	35.0	41.0	29.0	41.0
35	35.75318152808685	39.0	35.0	41.0	29.0	41.0
36	35.604711017557435	38.0	35.0	41.0	28.0	41.0
37	35.51801919976625	38.0	35.0	41.0	27.0	41.0
38	35.33346090653103	38.0	35.0	41.0	26.0	41.0
39	35.249482419761776	38.0	35.0	41.0	26.0	41.0
40	35.158408188278834	38.0	35.0	40.0	26.0	41.0
41	35.149427652450825	38.0	35.0	40.0	25.0	41.0
42	35.08373088502534	37.0	35.0	40.0	25.0	41.0
43	34.97166160317671	37.0	35.0	40.0	24.0	41.0
44	34.81059563994591	37.0	35.0	40.0	23.0	41.0
45	34.73821945539276	37.0	35.0	40.0	23.0	41.0
46	34.71432485828915	37.0	35.0	40.0	23.0	41.0
47	34.63372500362658	37.0	35.0	40.0	23.0	41.0
48	34.52518558985285	36.0	35.0	40.0	23.0	41.0
49	34.470571229568996	36.0	35.0	40.0	23.0	41.0
50	34.35666551096324	36.0	34.0	40.0	23.0	41.0
51	34.22857276113325	36.0	34.0	40.0	23.0	41.0
52	33.87044358778356	35.0	34.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	9.0
13	8.0
14	17.0
15	50.0
16	120.0
17	237.0
18	581.0
19	1165.0
20	2154.0
21	3550.0
22	5683.0
23	9201.0
24	16193.0
25	30478.0
26	47037.0
27	57002.0
28	59573.0
29	58030.0
30	58444.0
31	62914.0
32	72499.0
33	90920.0
34	196464.0
35	253688.0
36	312099.0
37	261092.0
38	382326.0
39	932794.0
40	1644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.48056069106264	15.806387782054223	22.18542489933892	35.527626627544215
2	39.76580030062007	17.491485689339374	22.53059270439061	20.212121305649948
3	23.91819128640766	19.220205399707062	38.15278810880622	18.70881520507906
4	21.376535379442814	21.65966557303514	36.8343602632809	20.129438784241145
5	19.924395733431002	28.65856439685827	33.6167035840181	17.80033628569263
6	71.96290912158652	1.7600300139953284	22.722501202857504	3.5545596615606523
7	70.78951005376251	1.6596861493573498	21.5030111732859	6.047792623594252
8	60.70591188601541	3.1147064804783855	27.583417267581012	8.595964365925198
9	37.3007911938828	13.853763392185044	30.97463522467458	17.870810189257583
10	25.609187739392645	12.972376630373464	41.36656958071971	20.051866049514175
11	23.302067611737147	10.541627100113752	43.67595310381817	22.480352184330926
12	20.641480562405757	11.508096954258493	46.186573058118164	21.663849425217585
13	16.34559030553438	14.23689451171187	46.876840080480854	22.540675102272896
14	14.57359173078763	15.383887299367998	48.655731723167534	21.386789246676837
15	17.662920747208567	14.430414822085117	46.87131876735484	21.035345663351478
16	18.00592803842834	18.485596402984527	43.75362872015619	19.754846838430947
17	17.291963951655244	19.538006696221125	44.11357718332783	19.0564521687958
18	18.642833798529683	25.75682285124039	39.133146980441865	16.46719636978806
19	19.535160304982245	21.320739252386765	37.69849720830749	21.445603234323503
20	18.062650099983777	26.06773107981453	37.43896119751452	18.43065762268718
21	17.14285420338254	20.76188634119726	37.755836559529186	24.339422895891012
22	18.29718587929312	22.330590852521613	35.90300733237242	23.469215935812848
23	18.100064712533346	19.408101515343247	39.256124799509465	23.235708972613946
24	21.69015282377443	18.0205715210669	36.994272580713194	23.29500307444548
25	16.321035894365277	17.561753829682235	40.707509980373615	25.40970029557887
26	16.262016143496528	17.426327335678348	40.80946565691795	25.502190863907177
27	20.343775473915567	18.723938801902488	39.95397076721904	20.978314956962908
28	22.24811409433489	20.470834263554565	34.885919725594164	22.395131916516373
29	21.0661415589239	18.04869249475218	38.14939301564178	22.735772930682145
30	23.928410859771336	16.408828202455922	36.14584222830595	23.51691870946679
31	24.69168953210472	17.636583054781372	35.8336308326586	21.838096580455307
32	24.656572608868462	16.275459340672906	38.14308294349777	20.924885106960865
33	18.700721851676953	18.769892588168684	42.31771007481894	20.211675485335427
34	22.682720313253927	21.723109233178768	37.05212633999012	18.542044113577184
35	25.944238852691708	22.505558179036637	32.53006800817428	19.02013496009737
36	27.652896648905873	19.512697819904368	32.187437949528345	20.64696758166142
37	26.429394236503562	21.704659130931596	32.41494348541636	19.451003147148484
38	26.26080556987325	17.597007928399886	33.83861921903941	22.30356728268746
39	25.831343431506397	15.758993653233414	39.332634424255644	19.077028491004548
40	20.13773790086534	15.683890077171498	42.24963674217834	21.928735279784828
41	21.566283364077787	19.698193364616202	35.78033815813795	22.95518511316806
42	20.32340491492891	15.982966920475599	37.68052722024518	26.01310094435031
43	22.540332163569417	18.269064905607838	38.13862474035253	21.051978190470212
44	22.056445652960434	18.844378874564338	37.69801709412262	21.401158378352612
45	22.02633563479497	19.97309302932503	36.03541596578569	21.965155370094305
46	22.996817871770418	18.033671779539798	35.94011330008885	23.02939704860093
47	21.6718056031383	17.90609858184558	36.41312865379755	24.00896716121857
48	22.28960967745586	16.045381764508793	34.0772016750498	27.587806882985543
49	21.311822846096312	15.232376980170942	38.290375116642025	25.165425057090722
50	21.483738018150376	14.023620932018233	40.026022188819994	24.4666188610114
51	19.199560489757623	13.374986668257902	40.27866513167303	27.14678771031145
52	15.658444025373349	14.531718915092833	43.80846461884249	26.001372440691323
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	785.0
1	4076.0
2	7367.0
3	134042.5
4	260718.0
5	146967.5
6	33217.0
7	30000.5
8	26784.0
9	25700.0
10	24616.0
11	23705.0
12	22794.0
13	21496.5
14	19100.5
15	18002.0
16	16614.0
17	15226.0
18	14328.0
19	13430.0
20	12478.5
21	11527.0
22	11073.0
23	10619.0
24	10395.5
25	10172.0
26	10818.0
27	11464.0
28	11540.0
29	11616.0
30	12357.5
31	13099.0
32	15696.0
33	18293.0
34	21058.5
35	23824.0
36	26632.5
37	29441.0
38	31275.5
39	35489.0
40	37868.0
41	44216.0
42	50564.0
43	66169.0
44	81774.0
45	113492.0
46	145210.0
47	200818.0
48	256426.0
49	290559.5
50	324693.0
51	313535.5
52	302378.0
53	259367.5
54	216357.0
55	188993.0
56	161629.0
57	146068.0
58	130507.0
59	122874.5
60	115242.0
61	113908.0
62	112574.0
63	114883.5
64	101251.0
65	85309.0
66	71095.0
67	56881.0
68	45693.0
69	34505.0
70	29013.0
71	23521.0
72	22931.5
73	22342.0
74	17424.0
75	12506.0
76	9231.0
77	5956.0
78	4585.0
79	3214.0
80	2453.5
81	1693.0
82	1194.5
83	696.0
84	528.5
85	361.0
86	246.0
87	131.0
88	106.5
89	57.0
90	32.0
91	23.5
92	15.0
93	12.5
94	10.0
95	5.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2915973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.078711131429408
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.66814205744502	22.459214756664245
2	9.601861207777453	5.776232191856319
3	3.6892882101022506	3.329071030567615
4	2.0594064886678174	2.4777717149932257
5	1.4151545636142142	2.1283012662638
6	1.0634730743988916	1.9192739640538439
7	0.895271643968564	1.8850032014163247
8	0.7764096766186892	1.8682721906128057
9	0.625725069866102	1.6938903261776372
>10	4.78183388630884	25.130720464797502
>50	0.20511573622484852	4.32666197179589
>100	0.20370785010212583	12.047981940255289
>500	0.008903294707920555	1.8084371656278742
>1k	0.004451647353960252	2.835586078431178
>5k	4.5657921579079504E-4	0.9751339847391007
>10k+	7.990136276338914E-4	9.338447751747305
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	199646	6.846634039478418	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	15115	0.5183518503086276	No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT	13680	0.46914014635937984	Illumina Single End Adapter 1 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	11738	0.40254145014374276	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCTT	11023	0.3780213328449886	Illumina Single End Adapter 1 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT	10507	0.36032569574546813	No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	10285	0.35271245652823263	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTC	9895	0.3393378470925485	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT	7215	0.24743027456015537	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCG	6260	0.21467962837790336	No Hit
GGGCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT	5032	0.17256675559067247	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT	4914	0.16852007888961937	Illumina Single End Adapter 1 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG	4830	0.1656393937803951	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCT	4686	0.16070107645029635	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3958	0.13573513883701943	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3510	0.12037148492115668	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTC	2981	0.10223002750711341	No Hit
AGGCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT	2945	0.10099544817458873	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.39019565681849594	0.0
2	0.0	0.0	0.0	1.175319524563499	0.0
3	0.0	0.0	0.0	2.010512443016448	0.0
4	0.0	0.0	0.0	2.6044136897015164	0.0
5	0.0	0.0	0.0	5.389864720969639	0.0
6	0.0	0.0	0.0	6.986724499849621	0.0
7	0.0	0.0	0.0	10.794235749096442	0.0
8	0.0	0.0	0.0	15.239372929721915	0.0
9	0.0	0.0	0.0	19.605737090158243	0.0
10	0.0	0.0	0.0	21.785626958823006	0.0
11	0.0	0.0	0.0	23.398947795469986	0.0
12	0.0	0.0	0.0	24.426735089796786	0.0
13	0.0	0.0	0.0	24.974854019567395	0.0
14	0.0	0.0	0.0	25.33473389499834	0.0
15	0.0	0.0	0.0	26.013341001442743	0.0
16	0.0	0.0	0.0	26.68807290053783	0.0
17	0.0	0.0	0.0	27.664590858694506	0.0
18	0.0	0.0	0.0	28.180953664522956	0.0
19	0.0	0.0	0.0	28.823689382583446	0.0
20	0.0	0.0	0.0	29.510595605652043	0.0
21	0.0	0.0	0.0	30.294999302119738	0.0
22	0.0	0.0	0.0	30.933311110905347	0.0
23	0.0	0.0	0.0	31.516238319079086	0.0
24	3.4293870347907885E-5	0.0	0.0	31.96891740767147	0.0
25	3.4293870347907885E-5	0.0	0.0	32.42674057681604	0.0
26	3.4293870347907885E-5	0.0	0.0	32.86655946402796	0.0
27	3.4293870347907885E-5	0.0	0.0	33.32822354665149	0.0
28	6.858774069581577E-5	0.0	0.0	33.76773378903028	0.0
29	6.858774069581577E-5	0.0	0.0	34.23245002611478	0.0
30	6.858774069581577E-5	0.0	0.0	34.651555415636565	0.0
31	6.858774069581577E-5	0.0	0.0	35.09003684190491	0.0
32	6.858774069581577E-5	0.0	0.0	35.58023342465791	0.0
33	6.858774069581577E-5	0.0	0.0	36.018646263185566	0.0
34	6.858774069581577E-5	0.0	0.0	36.51384975100935	0.0
35	6.858774069581577E-5	0.0	0.0	36.95263982211083	0.0
36	6.858774069581577E-5	0.0	0.0	37.440881654254	0.0
37	6.858774069581577E-5	0.0	0.0	38.04952926518867	0.0
38	1.0288161104372366E-4	0.0	0.0	38.72333522978436	0.0
39	1.0288161104372366E-4	0.0	0.0	39.389939481606994	0.0
40	1.0288161104372366E-4	0.0	0.0	39.97314104074352	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	47155	0.0	45.42445	1
ACTACGG	180	0.0	43.444447	1
CGGTCTA	395	0.0	43.088608	30
TACGGGA	260	0.0	42.46154	3
TAGTAGG	640	0.0	42.046875	1
GTTTTTT	51785	0.0	41.776188	2
TCACGAC	435	0.0	41.770115	24
CACGACG	420	0.0	41.61905	25
GTCGGCG	1830	0.0	41.475407	3
GCACTTA	3950	0.0	41.34177	35
CGCACTT	3965	0.0	41.18537	34
CTTCTTA	1855	0.0	41.164417	18
CACTTAC	3970	0.0	41.075565	36
GCGATAG	230	0.0	41.0	8
CGTTCTG	1175	0.0	40.91064	1
CGTTCAG	1535	0.0	40.905537	38
CCCGCAC	4030	0.0	40.86352	32
ACTTACT	3890	0.0	40.856045	37
ACCCGCA	4020	0.0	40.850742	31
GGCGATA	480	0.0	40.729168	7
>>END_MODULE