##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043377_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2853005
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31617750407027	33.0	31.0	34.0	31.0	34.0
2	32.6161191445511	34.0	31.0	34.0	31.0	34.0
3	32.6724243385483	34.0	31.0	34.0	31.0	34.0
4	36.19073187744151	37.0	37.0	37.0	35.0	37.0
5	36.17850126445625	37.0	37.0	37.0	35.0	37.0
6	36.34922511527319	37.0	37.0	37.0	35.0	37.0
7	36.296631446492384	37.0	37.0	37.0	35.0	37.0
8	36.20623272654622	37.0	37.0	37.0	35.0	37.0
9	37.85996624611594	39.0	38.0	39.0	35.0	39.0
10	37.693417291592546	39.0	38.0	39.0	35.0	39.0
11	37.44639774553497	39.0	37.0	39.0	35.0	39.0
12	37.45573491809513	39.0	37.0	39.0	35.0	39.0
13	37.45098098320893	39.0	37.0	39.0	35.0	39.0
14	38.729846950846564	40.0	38.0	41.0	35.0	41.0
15	38.826838368667424	41.0	38.0	41.0	35.0	41.0
16	38.78339785594487	41.0	38.0	41.0	35.0	41.0
17	38.69155995170005	41.0	38.0	41.0	34.0	41.0
18	38.44191229948773	40.0	38.0	41.0	34.0	41.0
19	38.166451162896664	40.0	37.0	41.0	34.0	41.0
20	37.762205814570954	40.0	35.0	41.0	34.0	41.0
21	37.729540256676735	39.0	35.0	41.0	34.0	41.0
22	37.72705060103294	39.0	35.0	41.0	34.0	41.0
23	37.7136664674615	39.0	35.0	41.0	34.0	41.0
24	37.64756037931935	39.0	35.0	41.0	34.0	41.0
25	37.62720535014835	39.0	35.0	41.0	34.0	41.0
26	37.61011424795961	39.0	35.0	41.0	34.0	41.0
27	37.58091731349928	39.0	35.0	41.0	34.0	41.0
28	37.5043920357658	39.0	35.0	41.0	34.0	41.0
29	37.4596486862098	39.0	35.0	41.0	34.0	41.0
30	37.35381150751576	39.0	35.0	41.0	34.0	41.0
31	37.1888945164835	39.0	35.0	41.0	33.0	41.0
32	36.89417579008799	39.0	35.0	41.0	33.0	41.0
33	36.358837085809526	39.0	35.0	41.0	31.0	41.0
34	36.04695890823886	39.0	35.0	41.0	30.0	41.0
35	35.839223205006654	39.0	35.0	41.0	29.0	41.0
36	35.68342712333136	38.0	35.0	41.0	29.0	41.0
37	35.55307894658439	38.0	35.0	41.0	28.0	41.0
38	35.38452158338313	38.0	35.0	41.0	27.0	41.0
39	35.26638298916406	38.0	35.0	40.0	26.0	41.0
40	35.15267340926497	38.0	35.0	40.0	26.0	41.0
41	35.09162234205689	38.0	35.0	40.0	25.0	41.0
42	35.01798104104269	37.0	35.0	40.0	25.0	41.0
43	34.899578164076125	37.0	35.0	40.0	24.0	41.0
44	34.80464072092408	37.0	35.0	40.0	23.0	41.0
45	34.7568139558115	37.0	35.0	40.0	23.0	41.0
46	34.67727080744689	37.0	35.0	40.0	23.0	41.0
47	34.54386515270741	36.0	35.0	40.0	23.0	41.0
48	34.458930496090964	36.0	34.0	40.0	23.0	41.0
49	34.388330199211005	36.0	34.0	40.0	23.0	41.0
50	34.30871204221514	36.0	34.0	40.0	23.0	41.0
51	33.90491464263119	35.0	34.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	3.0
13	11.0
14	26.0
15	37.0
16	97.0
17	197.0
18	442.0
19	855.0
20	1672.0
21	2975.0
22	4586.0
23	7409.0
24	12912.0
25	24774.0
26	42205.0
27	55374.0
28	58284.0
29	57058.0
30	56890.0
31	60499.0
32	68932.0
33	87865.0
34	188759.0
35	254023.0
36	296592.0
37	259115.0
38	399219.0
39	912031.0
40	160.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.8162165856702	15.912625459822188	22.278509851893006	34.99264810261461
2	39.45131536748095	17.565268900685417	22.618782651975724	20.364633079857906
3	24.071426443346578	19.370137802071852	37.72120974200887	18.837226012572707
4	21.533681153730893	21.813281084330384	36.35426506437949	20.29877269755924
5	20.17420228846427	28.851333944384955	33.14459666211591	17.829867105034864
6	72.5124912154027	1.7045185690175795	22.244125054109613	3.538865161470099
7	71.30709550105941	1.6400251664473071	20.979528602298277	6.0733507301950045
8	61.122746016919	3.0813125108438295	27.12876423280015	8.667177239437015
9	37.58945392664927	13.874003024880786	30.514457563165852	18.022085485304093
10	25.93518062534065	13.002045211978247	40.92376283953235	20.13901132314875
11	23.558283283765714	10.479091344039004	43.44955581921518	22.513069552980102
12	20.90045408262516	11.457603474231556	45.88751158865828	21.75443085448501
13	16.477433442983802	14.19159798177711	46.64254005863992	22.688428516599167
14	14.66523893228368	15.331378669157608	48.501281981629894	21.50210041692882
15	17.77175294119709	14.363241564595928	46.68260307991048	21.182402414296504
16	18.1187204368727	18.40396354019709	43.641248438050404	19.836067584879803
17	17.470106081132002	19.42260879318473	43.95989491781472	19.147390207868543
18	18.76901722920219	25.884952883012822	38.94034535516061	16.405684532624374
19	19.696635652583854	21.432559704592176	37.349776814271266	21.521027828552704
20	18.133196401688746	26.2820079179672	37.01038028324521	18.574415397098846
21	17.2242600345951	20.89712426020985	37.3661455202497	24.512470184945347
22	18.42849907378361	22.462841810652275	35.421669432755984	23.686989682808125
23	18.25401637922121	19.523800343847977	38.78762918396568	23.434554092965136
24	21.745037250197598	18.08118107048533	36.690436925277034	23.48334475404004
25	16.261310442848856	17.568879129198862	40.58440135926856	25.585409068683724
26	16.348341485556457	17.34168008818772	40.55765762765925	25.752320798596568
27	20.368874222092145	18.731127355192157	39.73512138955242	21.16487703316328
28	22.39000632666259	20.522606865392806	34.52335344662908	22.564033361315524
29	21.186328099670348	18.17266356000077	37.758188296199975	22.88282004412891
30	24.175702461089273	16.404597958994113	35.670494794085535	23.74920478583108
31	24.945417200460568	17.56901933224793	35.48395463730347	22.001608829988033
32	24.823545700060112	16.177048410360303	37.86684565922597	21.132560230353608
33	18.796146519196427	18.691730298404664	42.18499441816611	20.3271287642328
34	22.802308443202868	21.699155802390813	36.90964439249142	18.588891361914893
35	26.102442862876163	22.52852693913961	32.29044463644473	19.078585561539498
36	27.85382430104399	19.489836155211783	31.852240006589543	20.804099537154684
37	26.76451671132718	21.57251739832212	32.085888387857715	19.577077502492983
38	26.681691760091553	17.36172912420413	33.570007763743845	22.386571351960477
39	26.130869031074255	15.605791086941665	39.126885511942675	19.136454370041413
40	20.339116124927926	15.504284079418017	42.17430393567484	21.982295859979214
41	21.727511869064372	19.505433744420355	35.68307801773919	23.083976368776078
42	20.527584073634642	15.816691523498907	37.59194253077019	26.063781872096264
43	22.806794940772974	18.051002364173915	37.95468286946571	21.187519825587405
44	22.2083031750733	18.658607328062867	37.64998659308343	21.4831029037804
45	22.17419878338804	19.79695093419044	36.0519872905936	21.976862991827915
46	23.18036596500882	17.929551472920657	35.92871375970249	22.96136880236803
47	21.963017940732666	17.667862481839325	36.282270798684195	24.086848778743818
48	22.465961328493993	15.884970408393956	33.8928953857424	27.75617287736965
49	21.540516052372848	15.027768966405597	38.041118049214774	25.39059693200678
50	21.69631669064723	13.860508481408198	39.88878393132855	24.554390896616024
51	19.448441204975104	13.31631034645926	40.0104451271554	27.224803321410235
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	703.0
1	3918.0
2	7133.0
3	127260.0
4	247387.0
5	140672.0
6	33957.0
7	30106.5
8	26256.0
9	24953.5
10	23651.0
11	22752.5
12	21854.0
13	20604.5
14	19355.0
15	18005.0
16	16655.0
17	15549.5
18	14444.0
19	13385.0
20	12326.0
21	11506.0
22	10686.0
23	10294.0
24	9902.0
25	9674.5
26	9942.0
27	10437.0
28	10606.0
29	10775.0
30	12589.0
31	14403.0
32	16347.0
33	18291.0
34	21839.0
35	25387.0
36	28224.0
37	31061.0
38	32808.5
39	34556.0
40	38582.5
41	42609.0
42	51258.5
43	59908.0
44	75221.5
45	90535.0
46	133979.0
47	177423.0
48	239311.5
49	301200.0
50	328376.0
51	355552.0
52	307133.0
53	258714.0
54	220324.5
55	181935.0
56	161730.5
57	141526.0
58	131519.5
59	121513.0
60	118475.5
61	115438.0
62	113946.0
63	112454.0
64	109374.5
65	106295.0
66	87607.0
67	68919.0
68	54870.5
69	40822.0
70	33755.5
71	26689.0
72	24449.5
73	22210.0
74	18840.0
75	11533.5
76	7597.0
77	5710.5
78	3824.0
79	2919.0
80	2014.0
81	1461.5
82	909.0
83	675.5
84	442.0
85	307.0
86	172.0
87	144.0
88	116.0
89	73.0
90	30.0
91	21.5
92	13.0
93	11.0
94	9.0
95	4.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2853005.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.377772492856007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.55820727452284	21.60976278293278
2	9.925316014693967	5.831673515987594
3	3.8335260183569244	3.3786136563820164
4	2.1770660851945736	2.558294086110355
5	1.524660366757625	2.239556269173996
6	1.1521950725171506	2.030935482467915
7	0.9392934506584875	1.9316044508232302
8	0.8189364573451964	1.9246823143994125
9	0.6695066433858186	1.770175246665983
>10	4.957628710023941	25.27763802282785
>50	0.2157336427114039	4.452086140448331
>100	0.21376337590110422	12.471160560142446
>500	0.007923852571525747	1.587700453358544
>1k	0.004922393264129622	2.901631476105754
>5k	6.002918614792222E-4	1.2946733431396644
>10k+	7.203502337750667E-4	8.739812199034244
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	189272	6.6341278756959765	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	14402	0.5048010781614474	No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	13020	0.4563609247092101	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT	10888	0.38163269955713364	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG	10128	0.35499412023462984	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	10084	0.3534518866949059	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTC	9793	0.3432521148753683	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT	9330	0.3270236119460008	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCT	6879	0.241114193630926	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCC	5656	0.19824711137905474	No Hit
GGGCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTC	5049	0.17697129868331812	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	4811	0.16862921726390245	No Hit
CGTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT	4695	0.16456332884099398	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTC	4240	0.14861523200975812	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3847	0.13484028243904234	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3433	0.12032926686073105	No Hit
AGGCCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTC	2973	0.10420591621816296	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3931644003427964	0.0
2	0.0	0.0	0.0	1.1642461194424827	0.0
3	0.0	0.0	0.0	2.009004540826252	0.0
4	0.0	0.0	0.0	2.605533463839005	0.0
5	0.0	0.0	0.0	5.388423784746259	0.0
6	0.0	0.0	0.0	6.980814965273457	0.0
7	0.0	0.0	0.0	10.828267037737403	0.0
8	0.0	0.0	0.0	15.369268543167642	0.0
9	0.0	0.0	0.0	19.879951139237402	0.0
10	0.0	0.0	0.0	22.162211422692916	0.0
11	0.0	0.0	0.0	23.81695790929213	0.0
12	0.0	0.0	0.0	24.85775524403217	0.0
13	0.0	0.0	0.0	25.423719902348576	0.0
14	0.0	0.0	0.0	25.803494911505588	0.0
15	0.0	0.0	0.0	26.507910080774483	0.0
16	0.0	0.0	0.0	27.202721341182368	0.0
17	0.0	0.0	0.0	28.202824740931053	0.0
18	0.0	0.0	0.0	28.729462443984502	0.0
19	0.0	0.0	0.0	29.3895734497486	0.0
20	0.0	0.0	0.0	30.096337020089344	0.0
21	3.505076226645239E-5	0.0	0.0	30.896931481017383	0.0
22	3.505076226645239E-5	0.0	0.0	31.55139931405658	0.0
23	3.505076226645239E-5	0.0	0.0	32.14323143492563	0.0
24	3.505076226645239E-5	0.0	0.0	32.61203538023943	0.0
25	3.505076226645239E-5	0.0	0.0	33.066468513023985	0.0
26	3.505076226645239E-5	0.0	0.0	33.524932483469186	0.0
27	3.505076226645239E-5	0.0	0.0	33.99731160653416	0.0
28	3.505076226645239E-5	0.0	0.0	34.43656775925734	0.0
29	3.505076226645239E-5	0.0	0.0	34.904390283227684	0.0
30	3.505076226645239E-5	0.0	0.0	35.32577054719498	0.0
31	3.505076226645239E-5	0.0	0.0	35.76674418726921	0.0
32	3.505076226645239E-5	0.0	0.0	36.2596981077846	0.0
33	3.505076226645239E-5	0.0	0.0	36.707506646500796	0.0
34	3.505076226645239E-5	0.0	0.0	37.20820678547707	0.0
35	3.505076226645239E-5	0.0	0.0	37.647007278290786	0.0
36	3.505076226645239E-5	0.0	0.0	38.13838391450418	0.0
37	3.505076226645239E-5	0.0	0.0	38.75906281271852	0.0
38	3.505076226645239E-5	0.0	0.0	39.445216534846594	0.0
39	3.505076226645239E-5	0.0	0.0	40.13228157679359	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	44545	0.0	44.43428	1
CGCACTT	3740	0.0	41.991978	34
AATGCGG	265	0.0	41.603775	1
ACCCGCA	3900	0.0	41.480774	31
GCACTTA	3770	0.0	41.47878	35
CCGCACT	3790	0.0	41.319263	33
GTTTTTT	48545	0.0	41.009373	2
ACGTAGG	220	0.0	40.90909	1
CCCGCAC	3990	0.0	40.82707	32
CACTTAC	3850	0.0	40.79221	36
TAGTAGG	670	0.0	40.63433	1
CTTACGG	255	0.0	40.588234	1
TTATGAC	4485	0.0	40.183945	26
ACTTACT	3840	0.0	40.136723	37
CTTACTG	3825	0.0	40.117645	38
ATAGCGG	320	0.0	40.07813	1
CAACGCA	4690	0.0	40.010662	16
TATACGG	180	0.0	40.0	1
AACGCAA	4675	0.0	39.99465	17
CTTATGA	4515	0.0	39.916943	25
>>END_MODULE