FastQCFastQC Report
Thu 26 May 2016
SRR1043376_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043376_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2760023
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270184.6020630987495394No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT135020.4891988218938755TruSeq Adapter, Index 15 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC132550.4802496211082299No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCTT116840.4233298055849535TruSeq Adapter, Index 21 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA92090.3336566398178566No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT75900.2749977083524304TruSeq Adapter, Index 15 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC72040.2610123176509761TruSeq Adapter, Index 21 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT49000.17753475242778774No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG48500.17572317332138176No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC37780.13688291728003718No Hit
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT34850.126267063716498TruSeq Adapter, Index 15 (95% over 23bp)
GGGCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT33750.12228158968240481No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT33280.12057870532238318No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC32510.11778887349851795No Hit
CGTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG28350.10271653533322006No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC28020.10152089312299209No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT330850.045.3117751
CTAACGG1750.044.6857151
ACATACG1850.043.51351516
CGGTCTA4600.043.50000430
TCACGAC4750.042.61052324
CGCACTT33600.042.16666434
CACGACG4700.042.08510625
CCGCACT33650.042.03566433
ACCCGCA34650.042.01731531
CACTTAC33500.041.880636
TAGCATA10150.041.6945829
CCCGCAC35100.041.47863432
ACTTACT34000.041.1970637
TTGACGG3300.041.1212121
GTTGATC11950.040.8033515
GCACTTA34500.040.73333735
TAGTAGG5650.040.7079661
TACGGGT2150.040.651163
GTTTTTT377200.040.5670742
TTATGAC39300.040.4987326