##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043376_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2760023
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.327675167924326	33.0	31.0	34.0	31.0	34.0
2	32.65424418564628	34.0	31.0	34.0	31.0	34.0
3	32.765746517329745	34.0	31.0	34.0	31.0	34.0
4	36.21255547508119	37.0	37.0	37.0	35.0	37.0
5	36.13701081476495	37.0	37.0	37.0	35.0	37.0
6	36.30927713283549	37.0	37.0	37.0	35.0	37.0
7	36.24064980617915	37.0	37.0	37.0	35.0	37.0
8	36.22145902407335	37.0	37.0	37.0	35.0	37.0
9	37.8169863077228	39.0	38.0	39.0	35.0	39.0
10	37.630220110484586	39.0	37.0	39.0	35.0	39.0
11	37.27511328709942	39.0	37.0	39.0	35.0	39.0
12	37.28296032315673	39.0	37.0	39.0	35.0	39.0
13	37.25945291035618	39.0	37.0	39.0	34.0	39.0
14	38.600529053562234	40.0	38.0	41.0	35.0	41.0
15	38.65539272679974	40.0	38.0	41.0	35.0	41.0
16	38.51863299689894	40.0	38.0	41.0	34.0	41.0
17	38.51326565032248	40.0	38.0	41.0	34.0	41.0
18	38.29647832644873	40.0	37.0	41.0	34.0	41.0
19	38.08148772673271	40.0	37.0	41.0	34.0	41.0
20	37.813945391034785	40.0	35.0	41.0	34.0	41.0
21	37.72597510962771	40.0	35.0	41.0	34.0	41.0
22	37.67119078355506	39.0	35.0	41.0	34.0	41.0
23	37.69267393786211	39.0	35.0	41.0	34.0	41.0
24	37.61109019743676	39.0	35.0	41.0	34.0	41.0
25	37.506160637067154	39.0	35.0	41.0	34.0	41.0
26	37.43216451457108	39.0	35.0	41.0	34.0	41.0
27	37.464046132948894	39.0	35.0	41.0	34.0	41.0
28	37.43994597146473	39.0	35.0	41.0	34.0	41.0
29	37.350449253502596	39.0	35.0	41.0	33.0	41.0
30	37.25951885183566	39.0	35.0	41.0	33.0	41.0
31	37.13720103057112	39.0	35.0	41.0	33.0	41.0
32	36.84069009569848	39.0	35.0	41.0	32.0	41.0
33	36.45074008441234	39.0	35.0	41.0	31.0	41.0
34	36.19988529081098	39.0	35.0	41.0	30.0	41.0
35	35.95883621259678	39.0	35.0	41.0	30.0	41.0
36	35.9469359494468	39.0	35.0	41.0	30.0	41.0
37	35.80773348627892	38.0	35.0	41.0	30.0	41.0
38	35.67336576543021	38.0	35.0	41.0	29.0	41.0
39	35.625934638950476	38.0	35.0	41.0	29.0	41.0
40	35.60665653873174	38.0	35.0	40.0	29.0	41.0
41	35.5869338770003	38.0	35.0	40.0	29.0	41.0
42	35.53672704901372	38.0	35.0	40.0	29.0	41.0
43	35.46688234119788	38.0	35.0	40.0	29.0	41.0
44	35.32903348993831	38.0	35.0	40.0	28.0	41.0
45	35.227105353832194	37.0	35.0	40.0	27.0	41.0
46	35.179490170915244	37.0	35.0	40.0	26.0	41.0
47	35.079812740690926	37.0	35.0	40.0	26.0	41.0
48	34.987832710089734	37.0	35.0	40.0	26.0	41.0
49	34.9452504562462	37.0	35.0	40.0	27.0	41.0
50	34.82978909958359	36.0	35.0	40.0	26.0	41.0
51	34.710109662129625	36.0	35.0	40.0	26.0	41.0
52	34.325782792389774	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	3.0
13	11.0
14	12.0
15	43.0
16	92.0
17	239.0
18	517.0
19	1045.0
20	1957.0
21	3236.0
22	5380.0
23	8603.0
24	14722.0
25	26300.0
26	38940.0
27	46329.0
28	47815.0
29	47857.0
30	50426.0
31	57095.0
32	67212.0
33	86707.0
34	193630.0
35	261962.0
36	254982.0
37	247528.0
38	383387.0
39	912451.0
40	1539.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.307509393943455	16.867395670253472	23.70136045967733	30.12373447612574
2	35.58908748224199	19.272230702425304	24.043314131802525	21.09536768353017
3	25.47801232091182	20.56566195281706	34.73402214401837	19.222303582252756
4	23.097017669780286	23.404478875719516	32.46436714476655	21.03413630973365
5	21.295619637952292	30.227936506326213	29.656310835090867	18.820133020630625
6	75.84146943703006	2.1326996188075245	18.760568299611997	3.2652626445504254
7	75.51092871327522	2.0056716918663358	16.973517974306738	5.509881620551712
8	65.28043425725075	3.6151872647438084	23.186835761875898	7.91754271612954
9	39.66408975577377	15.160815688854768	27.07263671353463	18.102457841836824
10	28.54313170578651	16.283378797930308	36.80683095756811	18.366658538715075
11	25.361890100191193	13.35039599307687	39.687748978903436	21.5999649278285
12	20.79087746732545	14.256475398936894	42.716202002664474	22.236445131073182
13	16.0399750291936	17.088951794966924	44.49995525399607	22.371117921843407
14	17.122248618942667	17.662606434801447	43.27213939883834	21.943005547417542
15	20.54577081422872	14.629008526378223	43.231161479451444	21.594059179941617
16	19.056181778195327	19.518134450328855	39.98426824703997	21.441415524435847
17	20.433126825392396	19.65066957775352	38.882973076673636	21.03323052018045
18	19.93233389721752	26.249455167583747	35.94495408190439	17.87325685329434
19	21.148519414512126	21.58688532668025	34.38119899725473	22.883396261552893
20	20.542763592912088	26.42525080406939	34.13337497549839	18.89861062752013
21	18.009378907349685	20.514720348344923	36.994184468752614	24.481716275552774
22	19.600271447013302	22.2029671491868	33.96638361346989	24.230377790330007
23	20.258309441624217	20.205338868552907	37.393094187983216	22.14325750183966
24	22.631296913105434	18.799734639892492	34.27043905068907	24.298529396313
25	17.51318739010508	19.727263142372365	37.77019249477269	24.989356972749867
26	17.945321470147167	18.065211775409118	36.80947586306346	27.17999089138025
27	21.410980995448227	21.804709598434506	36.09433689501863	20.689972511098638
28	21.507393235491154	22.330828402516936	32.67994505842886	23.48183330356305
29	23.852880936137126	20.350663744468797	34.67612407577763	21.120331243616448
30	25.009393037666715	22.446443381087768	30.94727833789791	21.59688524334761
31	27.472452222318438	19.530851735655826	31.81995222503581	21.176743816989934
32	25.565402897004848	23.147596958431144	29.644970349884765	21.642029794679246
33	21.72083348580791	24.35657239088225	34.726812059174875	19.195782064134974
34	22.28952439889088	25.48199779494591	33.35508435980425	18.87339344635896
35	20.625154210671433	28.973635364632834	33.15218749988678	17.24902292480896
36	25.63623564006532	27.555784861213112	28.45273390837685	18.35524559034472
37	23.342269249205533	27.221657210827594	32.56795323807084	16.868120301896035
38	24.529505732379768	22.499667575233975	30.870141299547143	22.100685392839118
39	26.18195573007906	19.69842280299838	34.42540152745104	19.69421993947152
40	22.387168512726163	19.623350964828916	35.49071873676415	22.49876178568077
41	22.12260550002663	22.674231337927257	31.35285466824008	23.85030849380603
42	21.00004239095109	18.614699949964187	34.439241991823984	25.946015667260742
43	23.42397146690444	20.294070013184673	33.652871733315266	22.62908678659562
44	21.838404969813656	21.52250180523858	34.159824030451915	22.479269194495842
45	22.824121393191287	21.869890214682993	33.0234929201677	22.282495471958025
46	23.815018932813242	19.8298709829592	33.200592893609944	23.154517190617614
47	20.41997476107989	20.459757038256566	35.766223687266375	23.35404451339717
48	22.212387360540113	19.136253574698472	31.903429790258997	26.747929274502425
49	21.722826222824953	17.33496423761686	36.24422695028266	24.697982589275526
50	20.926890826634413	17.352029312799207	37.599288121874345	24.121791738692032
51	19.219803603085918	16.35174779340607	36.60150658164805	27.826942021859963
52	17.354964070951585	17.35561623942989	40.84581903846454	24.443600651153993
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	614.0
1	4175.5
2	7737.0
3	89322.0
4	170907.0
5	97277.0
6	23647.0
7	21569.5
8	19492.0
9	19079.0
10	18666.0
11	18127.0
12	17588.0
13	16993.0
14	15152.5
15	13907.0
16	12987.5
17	12068.0
18	11319.5
19	10571.0
20	9921.0
21	9271.0
22	8750.5
23	8230.0
24	8480.5
25	8731.0
26	9552.5
27	10374.0
28	10913.5
29	11453.0
30	12905.0
31	14357.0
32	16960.0
33	19563.0
34	22891.5
35	26220.0
36	28753.0
37	31286.0
38	35281.5
39	45900.5
40	52524.0
41	68271.5
42	84019.0
43	116972.5
44	149926.0
45	200084.0
46	250242.0
47	276273.0
48	302304.0
49	287310.0
50	272316.0
51	241400.5
52	210485.0
53	182158.5
54	153832.0
55	140444.0
56	127056.0
57	121837.0
58	116618.0
59	114518.0
60	112418.0
61	111022.0
62	109626.0
63	107445.5
64	88496.0
65	71727.0
66	57998.5
67	44270.0
68	37840.0
69	31410.0
70	27817.0
71	24224.0
72	22919.5
73	21615.0
74	18095.5
75	14576.0
76	11247.0
77	7918.0
78	5801.0
79	3684.0
80	2830.0
81	1976.0
82	1438.0
83	900.0
84	635.0
85	370.0
86	304.0
87	238.0
88	163.5
89	55.0
90	21.0
91	15.0
92	9.0
93	7.0
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2760023.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.354222539252067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.9856977196916	21.42438413046409
2	11.287690149996529	6.626827372342432
3	4.343509592358203	3.825010415263762
4	2.1722826015076637	2.550626676112055
5	1.3201764072698963	1.9376376025035402
6	0.8890908945235035	1.5659143185279365
7	0.6648275748266165	1.3660847607184228
8	0.520802657648569	1.223020568932
9	0.44855825067847904	1.185037084101032
>10	4.844467892539131	30.375142839955032
>50	0.331805772472994	6.5139624075188545
>100	0.17657575814895254	9.7790863294387
>500	0.00831185297974132	1.5883253472297485
>1k	0.005334472807893678	3.1394698392797085
>5k	3.7217252148095427E-4	0.8740960498339057
>10k+	4.962300286412724E-4	6.025374257778952
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	127018	4.6020630987495394	No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	13502	0.4891988218938755	TruSeq Adapter, Index 15 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	13255	0.4802496211082299	No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCTT	11684	0.4233298055849535	TruSeq Adapter, Index 21 (95% over 23bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	9209	0.3336566398178566	No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	7590	0.2749977083524304	TruSeq Adapter, Index 15 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	7204	0.2610123176509761	TruSeq Adapter, Index 21 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT	4900	0.17753475242778774	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG	4850	0.17572317332138176	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	3778	0.13688291728003718	No Hit
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	3485	0.126267063716498	TruSeq Adapter, Index 15 (95% over 23bp)
GGGCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT	3375	0.12228158968240481	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT	3328	0.12057870532238318	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3251	0.11778887349851795	No Hit
CGTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	2835	0.10271653533322006	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2802	0.10152089312299209	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.43695288046512654	0.0
2	0.0	0.0	0.0	1.4037926495540074	0.0
3	0.0	0.0	0.0	2.2707057151335333	0.0
4	0.0	0.0	0.0	2.8322227749551363	0.0
5	0.0	0.0	0.0	5.719771175819911	0.0
6	0.0	0.0	0.0	6.752661119128355	0.0
7	0.0	0.0	0.0	9.763795446632148	0.0
8	0.0	0.0	0.0	13.158948313111884	0.0
9	0.0	0.0	0.0	16.419609546732037	0.0
10	0.0	0.0	0.0	18.187203512434497	0.0
11	0.0	0.0	0.0	19.683531622743722	0.0
12	0.0	0.0	0.0	20.74276192625931	0.0
13	0.0	0.0	0.0	21.28192409990786	0.0
14	0.0	0.0	0.0	21.625471961646696	0.0
15	0.0	0.0	0.0	22.5055008599566	0.0
16	0.0	0.0	0.0	23.50299254752587	0.0
17	0.0	0.0	0.0	24.670772671097307	0.0
18	0.0	0.0	0.0	25.389788418429845	0.0
19	0.0	0.0	0.0	26.190433920297043	0.0
20	3.6231582128119945E-5	0.0	0.0	27.059919428207664	0.0
21	3.6231582128119945E-5	0.0	0.0	27.932303462688534	0.0
22	3.6231582128119945E-5	0.0	0.0	28.739289491428153	0.0
23	3.6231582128119945E-5	0.0	0.0	29.465768944679084	0.0
24	1.0869474638435984E-4	0.0	0.0	30.062321944418578	0.0
25	1.0869474638435984E-4	0.0	0.0	30.633657762996904	0.0
26	1.0869474638435984E-4	0.0	0.0	31.214522487674923	0.0
27	1.4492632851247978E-4	0.0	0.0	31.81252475069954	0.0
28	1.4492632851247978E-4	0.0	0.0	32.402121286670436	0.0
29	1.8115791064059973E-4	0.0	0.0	33.0368986055551	0.0
30	1.8115791064059973E-4	0.0	0.0	33.612582213988794	0.0
31	1.8115791064059973E-4	0.0	0.0	34.18112820074325	0.0
32	1.8115791064059973E-4	0.0	0.0	34.81793448822709	0.0
33	1.8115791064059973E-4	0.0	0.0	35.41673384605853	0.0
34	1.8115791064059973E-4	0.0	0.0	36.05172855443596	0.0
35	1.8115791064059973E-4	0.0	0.0	36.62092670966872	0.0
36	2.1738949276871967E-4	0.0	0.0	37.177842358560056	0.0
37	2.1738949276871967E-4	0.0	0.0	37.882546630951985	0.0
38	2.1738949276871967E-4	0.0	0.0	38.5820697870996	0.0
39	2.1738949276871967E-4	0.0	0.0	39.41778746046681	0.0
40	2.1738949276871967E-4	0.0	0.0	40.155063925191925	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	33085	0.0	45.311775	1
CTAACGG	175	0.0	44.685715	1
ACATACG	185	0.0	43.513515	16
CGGTCTA	460	0.0	43.500004	30
TCACGAC	475	0.0	42.610523	24
CGCACTT	3360	0.0	42.166664	34
CACGACG	470	0.0	42.085106	25
CCGCACT	3365	0.0	42.035664	33
ACCCGCA	3465	0.0	42.017315	31
CACTTAC	3350	0.0	41.8806	36
TAGCATA	1015	0.0	41.69458	29
CCCGCAC	3510	0.0	41.478634	32
ACTTACT	3400	0.0	41.19706	37
TTGACGG	330	0.0	41.121212	1
GTTGATC	1195	0.0	40.80335	15
GCACTTA	3450	0.0	40.733337	35
TAGTAGG	565	0.0	40.707966	1
TACGGGT	215	0.0	40.65116	3
GTTTTTT	37720	0.0	40.567074	2
TTATGAC	3930	0.0	40.49873	26
>>END_MODULE