FastQCFastQC Report
Thu 26 May 2016
SRR1043375_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043375_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2724864
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1202874.414422150977076No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC131500.4825928927095077TruSeq Adapter, Index 15 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC126740.46512413096580235No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT111680.4098553175497933TruSeq Adapter, Index 21 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG78840.28933554114994364No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC75630.2775551366967306TruSeq Adapter, Index 15 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG72400.26570133408493046No Hit
CGTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC46890.17208198280721534No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC45720.16778819052987598No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT36590.1342819311349117No Hit
GGGCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC34340.12602463829387447No Hit
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC34110.12518055947012402TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT32320.11861142427658776No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC31480.11552870161593387No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC28290.10382169532130778No Hit
CGTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT27980.10268402386320932No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGTA253.8922182E-545.00000444
CGTTTTT307600.044.4294551
CGGTCTA3700.042.56756630
TCACGAC4000.041.62500424
TATACGG1200.041.2500041
CACTTAC35350.041.18104636
CGCACTT35200.041.16477234
ATTACGG2250.041.01
CTTGCGG7250.040.9655151
CACGACG3900.040.96153625
CCGCACT35500.040.6901433
TAGTAGG5200.040.6730771
ACCCGCA37000.040.5608131
ACTTACT35750.040.40559437
CGACGGT3900.040.38461327
CGGGTAC4350.040.344835
CCCGCAC37150.040.27590632
CTAGCGG7550.040.2317921
TACGGGA4650.040.1612853
AATGCGG3150.040.01
Produced by FastQC (version 0.11.5)