##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043375_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2724864
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.287975106280534	33.0	31.0	34.0	31.0	34.0
2	32.57705118493987	34.0	31.0	34.0	31.0	34.0
3	32.671778114430666	34.0	31.0	34.0	31.0	34.0
4	36.17236970358888	37.0	37.0	37.0	35.0	37.0
5	36.147395246148065	37.0	37.0	37.0	35.0	37.0
6	36.32336659737881	37.0	37.0	37.0	35.0	37.0
7	36.26731132269354	37.0	37.0	37.0	35.0	37.0
8	36.17203684293968	37.0	37.0	37.0	35.0	37.0
9	37.77044138716648	39.0	38.0	39.0	35.0	39.0
10	37.57022258725573	39.0	37.0	39.0	35.0	39.0
11	37.26191252113867	39.0	37.0	39.0	35.0	39.0
12	37.28078685761932	39.0	37.0	39.0	35.0	39.0
13	37.26829155510146	39.0	37.0	39.0	34.0	39.0
14	38.601097889656145	40.0	38.0	41.0	35.0	41.0
15	38.66556165738914	40.0	38.0	41.0	35.0	41.0
16	38.57228324055806	40.0	38.0	41.0	34.0	41.0
17	38.5419297990652	40.0	38.0	41.0	34.0	41.0
18	38.348731900014094	40.0	37.0	41.0	34.0	41.0
19	38.150929000493235	40.0	37.0	41.0	34.0	41.0
20	37.90776347003006	40.0	35.0	41.0	34.0	41.0
21	37.817582088500565	40.0	35.0	41.0	34.0	41.0
22	37.783686818865085	40.0	35.0	41.0	34.0	41.0
23	37.76552407753194	40.0	35.0	41.0	34.0	41.0
24	37.68888428927095	39.0	35.0	41.0	34.0	41.0
25	37.65341352816141	39.0	35.0	41.0	34.0	41.0
26	37.637123540844605	39.0	35.0	41.0	34.0	41.0
27	37.609588588641486	39.0	35.0	41.0	34.0	41.0
28	37.5390988320885	39.0	35.0	41.0	34.0	41.0
29	37.47774934822435	39.0	35.0	41.0	34.0	41.0
30	37.339030131412066	39.0	35.0	41.0	33.0	41.0
31	37.210984107830704	39.0	35.0	41.0	33.0	41.0
32	36.926940573914884	39.0	35.0	41.0	33.0	41.0
33	36.521406572951896	39.0	35.0	41.0	31.0	41.0
34	36.23815280322247	39.0	35.0	41.0	31.0	41.0
35	36.00337374635945	39.0	35.0	41.0	30.0	41.0
36	35.96198856163097	39.0	35.0	41.0	30.0	41.0
37	35.78796409655675	38.0	35.0	41.0	30.0	41.0
38	35.657235003288235	38.0	35.0	40.0	29.0	41.0
39	35.56265890701334	38.0	35.0	40.0	29.0	41.0
40	35.51601657917606	38.0	35.0	40.0	29.0	41.0
41	35.45409495666573	38.0	35.0	40.0	29.0	41.0
42	35.38246899661781	38.0	35.0	40.0	28.0	41.0
43	35.29279112645622	38.0	35.0	40.0	27.0	41.0
44	35.19935086668546	37.0	35.0	40.0	27.0	41.0
45	35.12916167559188	37.0	35.0	40.0	26.0	41.0
46	35.03122761356163	37.0	35.0	40.0	26.0	41.0
47	34.876506497205	37.0	35.0	40.0	25.0	41.0
48	34.812329349281285	37.0	35.0	40.0	25.0	41.0
49	34.75503841659621	36.0	35.0	40.0	26.0	41.0
50	34.670085185902856	36.0	35.0	40.0	26.0	41.0
51	34.24145608734968	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	1.0
12	3.0
13	6.0
14	16.0
15	38.0
16	102.0
17	199.0
18	454.0
19	955.0
20	1810.0
21	2972.0
22	4758.0
23	7612.0
24	12402.0
25	22537.0
26	36291.0
27	45809.0
28	48499.0
29	48094.0
30	51248.0
31	57229.0
32	65845.0
33	85438.0
34	188560.0
35	262509.0
36	244358.0
37	247403.0
38	401701.0
39	887867.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.593036569898533	16.990682837748967	23.830987528184895	29.585293064167605
2	35.255043921458096	19.41920771091695	24.121020351841413	21.204728015783537
3	25.600250140924462	20.722979201897783	34.37030251785043	19.306468139327322
4	23.366046892615557	23.537651787391958	31.950328530157833	21.145972789834648
5	21.507422021796316	30.30499870819241	29.267405639327322	18.920173630683955
6	76.25518190999624	2.114747745208568	18.34425497933108	3.2858153654641113
7	75.89483365041337	1.9821906708004509	16.568459930477264	5.554515748308907
8	65.62573398158587	3.5597372933107856	22.80510146561443	8.009427259488914
9	39.90000968855693	15.183326580702744	26.702947376456223	18.213716354284102
10	28.899387272172117	16.293216835776025	36.40519306651635	18.402202825535515
11	25.588396338312663	13.380704504885383	39.424242824596014	21.606656332205937
12	21.00178210729049	14.253628804960542	42.437273933671555	22.307315154077415
13	16.17034831830139	17.10683542371289	44.25237369644871	22.470442561537016
14	17.21928140266817	17.650348788049605	43.05796546176249	22.07240434751973
15	20.657030956407365	14.677833462514092	42.9625478555994	21.702587725479145
16	19.193801965896277	19.503358699736943	39.78690312617437	21.51593620819241
17	20.68528924746336	19.63749383455468	38.6475802095077	21.029636708474257
18	20.067717141112364	26.40924464487035	35.65256834836528	17.870469865652012
19	21.288915703682825	21.653410959225855	34.01193600854941	23.0457373285419
20	20.600073985343855	26.61053909479519	33.75540944428786	19.033977475573096
21	18.124243998966556	20.57577919485156	36.652361365558065	24.647615440623827
22	19.68795506858324	22.35561114242766	33.5536746054115	24.4027591835776
23	20.403733911123638	20.27473664740699	37.019021866779404	22.30250757468997
24	22.694196847989478	18.81994844513341	34.02459719090567	24.46125751597144
25	17.48549652386321	19.72160812429538	37.63108911123638	25.161806240605035
26	18.048313603908305	18.0460749600714	36.571072904922964	27.33453853109733
27	21.49006335729049	21.81664846392334	35.874194088218715	20.819094090567454
28	21.563608312194663	22.43124794485156	32.387487962702	23.617655780251784
29	24.00633572904923	20.380503393930855	34.3236212889891	21.289539588030813
30	25.179825488538143	22.45330409150695	30.58435209977452	21.782518320180383
31	27.73070509207065	19.495872087561068	31.41114565717775	21.36227716319053
32	25.721503898910186	23.243655463171738	29.25573533211199	21.779105305806088
33	21.933681827790306	24.355380672209694	34.421534432544156	19.289403067455844
34	22.463396338312663	25.622673278372794	33.0416857501879	18.872244633126645
35	20.706134324502067	29.112829117343104	32.92619374765126	17.254842810503572
36	25.775231350995863	27.648939543404733	28.175020845077036	18.40080826052236
37	23.492144929068022	27.247304819616687	32.34139391910936	16.919156332205937
38	24.792760299229613	22.46783692690718	30.61349116873356	22.12591160512965
39	26.450310914599772	19.65701774473882	34.241892439402484	19.650778901258924
40	22.604100608323936	19.570481315764752	35.25775965332581	22.56765842258549
41	22.369630190717775	22.543437030251784	31.172858535325066	23.914074243705375
42	21.19852587138294	18.55784362081924	34.29829892427659	25.94533158352123
43	23.651675826756858	20.206402961762496	33.45256864195791	22.689352569522736
44	21.994749095734687	21.463860214674934	34.03226729847801	22.509123391112364
45	23.057701228391583	21.760315377207817	32.89896303081548	22.28302036358512
46	24.094156625798572	19.780730341037202	32.955516312006765	23.16959672115746
47	20.668994856257047	20.335510322717024	35.56364647923713	23.431848341788804
48	22.426440365464114	18.998232572341227	31.63181722096956	26.943509841225104
49	21.872027374577225	17.165333756106726	36.14701504368659	24.815623825629462
50	21.14325705796693	17.191353403325817	37.44190535747839	24.223484181228862
51	19.35817714205186	16.409956607008645	36.29282048571966	27.939045765219845
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	547.0
1	3949.5
2	7352.0
3	84462.5
4	161573.0
5	92993.0
6	24413.0
7	21672.5
8	18932.0
9	18577.5
10	18223.0
11	17776.5
12	17330.0
13	16461.0
14	15592.0
15	14426.0
16	13260.0
17	12274.0
18	11288.0
19	10407.0
20	9526.0
21	9052.0
22	8578.0
23	8111.0
24	7644.0
25	7880.5
26	9420.0
27	10723.0
28	11083.5
29	11444.0
30	13233.0
31	15022.0
32	17844.5
33	20667.0
34	24369.0
35	28071.0
36	30814.0
37	33557.0
38	38544.0
39	43531.0
40	53306.5
41	63082.0
42	80348.0
43	97614.0
44	138252.5
45	178891.0
46	232104.5
47	285318.0
48	308163.0
49	331008.0
50	291632.0
51	252256.0
52	213556.5
53	174857.0
54	154743.5
55	134630.0
56	126848.0
57	119066.0
58	117223.0
59	115380.0
60	112544.0
61	109708.0
62	107157.0
63	104606.0
64	99582.0
65	94558.0
66	75168.5
67	55779.0
68	46452.5
69	37126.0
70	32456.0
71	27786.0
72	25175.5
73	22565.0
74	19513.0
75	13181.5
76	9902.0
77	7081.5
78	4261.0
79	3361.0
80	2461.0
81	1865.0
82	1269.0
83	873.0
84	477.0
85	372.0
86	267.0
87	176.5
88	86.0
89	62.0
90	38.0
91	24.5
92	11.0
93	8.0
94	5.0
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2724864.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.91887002745105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.11985419602568	20.856246898935872
2	11.627466339754715	6.725063756558572
3	4.469552673294577	3.877632385195567
4	2.2571252186628654	2.610940433367737
5	1.3730502805644962	1.985353130239993
6	0.9145038914793214	1.5867851506373216
7	0.689080322668219	1.3949197002801377
8	0.5489937033917157	1.2701022043419232
9	0.46188884784473605	1.202157320215593
>10	5.002935781248865	30.61187763345397
>50	0.3383561179548499	6.518261747904347
>100	0.18248911182969096	9.992307523995676
>500	0.00869425251353349	1.6956967060758579
>1k	0.005114266184431463	3.018466590288234
>5k	3.8356996383235974E-4	0.838845013644338
>10k+	5.114266184431462E-4	5.8153438048648045
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	120287	4.414422150977076	No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	13150	0.4825928927095077	TruSeq Adapter, Index 15 (95% over 22bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	12674	0.46512413096580235	No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	11168	0.4098553175497933	TruSeq Adapter, Index 21 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	7884	0.28933554114994364	No Hit
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	7563	0.2775551366967306	TruSeq Adapter, Index 15 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG	7240	0.26570133408493046	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	4689	0.17208198280721534	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCC	4572	0.16778819052987598	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTT	3659	0.1342819311349117	No Hit
GGGCCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTC	3434	0.12602463829387447	No Hit
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	3411	0.12518055947012402	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCT	3232	0.11861142427658776	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	3148	0.11552870161593387	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTC	2829	0.10382169532130778	No Hit
CGTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCT	2798	0.10268402386320932	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.42273669438181133	0.0
2	0.0	0.0	0.0	1.3893904429725668	0.0
3	3.669907929349868E-5	0.0	0.0	2.261470664223976	0.0
4	3.669907929349868E-5	0.0	0.0	2.8382700934798946	0.0
5	3.669907929349868E-5	0.0	0.0	5.752213688462984	0.0
6	3.669907929349868E-5	0.0	0.0	6.801183471909057	0.0
7	3.669907929349868E-5	0.0	0.0	9.88342904453213	0.0
8	3.669907929349868E-5	0.0	0.0	13.365841377771515	0.0
9	3.669907929349868E-5	0.0	0.0	16.72428422115746	0.0
10	3.669907929349868E-5	0.0	0.0	18.54830186020293	0.0
11	3.669907929349868E-5	0.0	0.0	20.07079986377302	0.0
12	3.669907929349868E-5	0.0	0.0	21.15709261086058	0.0
13	3.669907929349868E-5	0.0	0.0	21.714992014280345	0.0
14	3.669907929349868E-5	0.0	0.0	22.076477945321308	0.0
15	3.669907929349868E-5	0.0	0.0	22.98573433389703	0.0
16	3.669907929349868E-5	0.0	0.0	23.994665421833897	0.0
17	3.669907929349868E-5	0.0	0.0	25.196009782506575	0.0
18	3.669907929349868E-5	0.0	0.0	25.941551578354	0.0
19	3.669907929349868E-5	0.0	0.0	26.770657177752724	0.0
20	3.669907929349868E-5	0.0	0.0	27.659655674558437	0.0
21	3.669907929349868E-5	0.0	0.0	28.552030486659152	0.0
22	3.669907929349868E-5	0.0	0.0	29.374640349022922	0.0
23	3.669907929349868E-5	0.0	0.0	30.12627419203307	0.0
24	1.1009723788049605E-4	0.0	0.0	30.746451933013905	0.0
25	1.1009723788049605E-4	0.0	0.0	31.335839146467492	0.0
26	1.1009723788049605E-4	0.0	0.0	31.93495161593386	0.0
27	1.1009723788049605E-4	0.0	0.0	32.54929420330703	0.0
28	1.1009723788049605E-4	0.0	0.0	33.142791713641486	0.0
29	1.1009723788049605E-4	0.0	0.0	33.77280480787298	0.0
30	1.1009723788049605E-4	0.0	0.0	34.33841101794438	0.0
31	1.1009723788049605E-4	0.0	0.0	34.91678850760992	0.0
32	1.1009723788049605E-4	0.0	0.0	35.56445385898159	0.0
33	1.1009723788049605E-4	0.0	0.0	36.1683739078354	0.0
34	1.1009723788049605E-4	0.0	0.0	36.80671769306652	0.0
35	1.1009723788049605E-4	0.0	0.0	37.377131482525364	0.0
36	1.1009723788049605E-4	0.0	0.0	37.94119633126644	0.0
37	1.4679631717399473E-4	0.0	0.0	38.65539711339722	0.0
38	1.8349539646749343E-4	0.0	0.0	39.359579046880874	0.0
39	1.8349539646749343E-4	0.0	0.0	40.196941939120634	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGTA	25	3.8922182E-5	45.000004	44
CGTTTTT	30760	0.0	44.429455	1
CGGTCTA	370	0.0	42.567566	30
TCACGAC	400	0.0	41.625004	24
TATACGG	120	0.0	41.250004	1
CACTTAC	3535	0.0	41.181046	36
CGCACTT	3520	0.0	41.164772	34
ATTACGG	225	0.0	41.0	1
CTTGCGG	725	0.0	40.965515	1
CACGACG	390	0.0	40.961536	25
CCGCACT	3550	0.0	40.69014	33
TAGTAGG	520	0.0	40.673077	1
ACCCGCA	3700	0.0	40.56081	31
ACTTACT	3575	0.0	40.405594	37
CGACGGT	390	0.0	40.384613	27
CGGGTAC	435	0.0	40.34483	5
CCCGCAC	3715	0.0	40.275906	32
CTAGCGG	755	0.0	40.231792	1
TACGGGA	465	0.0	40.161285	3
AATGCGG	315	0.0	40.0	1
>>END_MODULE