FastQCFastQC Report
Thu 26 May 2016
SRR1043374_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043374_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences629924
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440326.99004959328427No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT54900.8715337088283666TruSeq Adapter, Index 14 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCTT48830.7751728779979806Illumina PCR Primer Index 8 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT33950.5389539055505109TruSeq Adapter, Index 14 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC26060.4137007004019533No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG16440.2609838647201885No Hit
TCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT15930.25288764993872276TruSeq Adapter, Index 14 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC13430.2132003225785968TruSeq Adapter, Index 14 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT11950.1897054247814022No Hit
ACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC10940.17367174452791131No Hit
AGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC9450.15001809742127623No Hit
CGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG8560.13588940888107137No Hit
TCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC8540.13557191026219037No Hit
ACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT8050.12779319409960566Illumina PCR Primer Index 8 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT7740.12287196550695005No Hit
CGTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG7650.1214432217219855No Hit
TGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCTTG7240.11493450003492484TruSeq Adapter, Index 14 (96% over 25bp)
GGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC7190.11414075348772233TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT7180.11398200417828183No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC7100.1127120097027578No Hit
TGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC7010.11128326591779325No Hit
ATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC6330.10048831287583898No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC6320.1003295635663985No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATGG551.8189894E-1246.0000041
TTCACGG351.0185795E-746.0000041
TCGATGG351.0185795E-746.0000041
TGGCCGC551.8189894E-1246.00000436
GTCACGG551.8189894E-1246.0000041
TGGATAG551.8189894E-1246.00000441
GTGACGG351.0185795E-746.0000041
CGTTTTC551.8189894E-1246.0000041
CTAATGG351.0185795E-746.0000041
CCTTCCG206.309271E-446.014
GTCATAA301.8600531E-646.044
AACGTGG301.8600531E-646.01
CGGGTAG206.309271E-446.05
CTGTCCG206.309271E-446.036
TGCCCCG206.309271E-446.042
CAAAAGT206.309271E-446.036
CGACGGT301.8600531E-646.027
CATGCGG800.046.01
TTAGCGC206.309271E-446.01
TCCGAGG501.6370905E-1146.01
Produced by FastQC (version 0.11.5)