##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043374_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	629924
Sequences flagged as poor quality	0
Sequence length	52
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22667020148462	33.0	31.0	34.0	30.0	34.0
2	32.55151415091344	34.0	31.0	34.0	31.0	34.0
3	32.60706529676596	34.0	31.0	34.0	31.0	34.0
4	36.13877229634051	37.0	35.0	37.0	35.0	37.0
5	36.019567439881634	37.0	35.0	37.0	35.0	37.0
6	36.272259828169744	37.0	37.0	37.0	35.0	37.0
7	36.1884290803335	37.0	37.0	37.0	35.0	37.0
8	36.175357662194166	37.0	37.0	37.0	35.0	37.0
9	37.75734056806853	39.0	38.0	39.0	35.0	39.0
10	37.564099796165884	39.0	37.0	39.0	35.0	39.0
11	37.165175481486656	39.0	37.0	39.0	34.0	39.0
12	37.126504149706946	39.0	37.0	39.0	33.0	39.0
13	37.14628272617014	39.0	37.0	39.0	34.0	39.0
14	38.52690800795016	40.0	37.0	41.0	34.0	41.0
15	38.63895327055327	40.0	38.0	41.0	35.0	41.0
16	38.663424476603524	40.0	38.0	41.0	35.0	41.0
17	38.64983712320851	40.0	37.0	41.0	35.0	41.0
18	38.362321486401534	40.0	37.0	41.0	35.0	41.0
19	38.07725535143922	40.0	37.0	41.0	34.0	41.0
20	37.75600866136232	39.0	35.0	41.0	34.0	41.0
21	37.6943313796585	39.0	35.0	41.0	34.0	41.0
22	37.65026098386472	39.0	35.0	41.0	34.0	41.0
23	37.66388961208019	39.0	35.0	41.0	34.0	41.0
24	37.55992945180689	39.0	35.0	41.0	34.0	41.0
25	37.46524501368419	39.0	35.0	41.0	34.0	41.0
26	37.42172230300798	39.0	35.0	41.0	34.0	41.0
27	37.46683409427169	39.0	35.0	41.0	34.0	41.0
28	37.39343793854497	39.0	35.0	41.0	33.0	41.0
29	37.35783364342365	39.0	35.0	41.0	33.0	41.0
30	37.19093414443647	39.0	35.0	41.0	33.0	41.0
31	37.08081927343616	39.0	35.0	41.0	33.0	41.0
32	36.75447038055385	39.0	35.0	41.0	32.0	41.0
33	36.52490617915812	39.0	35.0	41.0	31.0	41.0
34	36.342681021837556	39.0	35.0	41.0	31.0	41.0
35	36.03540109600523	39.0	35.0	41.0	30.0	41.0
36	36.05967704040487	39.0	35.0	41.0	30.0	41.0
37	35.954724696947565	39.0	35.0	41.0	30.0	41.0
38	35.80986118960382	38.0	35.0	41.0	29.0	41.0
39	35.79246385278224	38.0	35.0	41.0	29.0	41.0
40	35.77407274528356	38.0	35.0	40.0	29.0	41.0
41	35.77250588959938	38.0	35.0	41.0	29.0	41.0
42	35.649271975666906	38.0	35.0	40.0	29.0	41.0
43	35.50715165639029	38.0	35.0	40.0	28.0	41.0
44	35.276831808281635	37.0	35.0	40.0	28.0	41.0
45	35.040415986690455	37.0	35.0	40.0	28.0	41.0
46	34.84403515344708	37.0	35.0	39.0	27.0	41.0
47	34.57918415554892	36.0	35.0	39.0	26.0	41.0
48	34.374056235355376	36.0	35.0	39.0	26.0	40.0
49	34.07034816898547	35.0	35.0	37.0	26.0	40.0
50	33.7676211733479	35.0	34.0	37.0	26.0	40.0
51	33.59291438332243	35.0	34.0	37.0	26.0	40.0
52	33.20007175468787	35.0	33.0	37.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	1.0
14	4.0
15	8.0
16	13.0
17	46.0
18	111.0
19	230.0
20	453.0
21	762.0
22	1254.0
23	2044.0
24	3721.0
25	6645.0
26	9286.0
27	10127.0
28	9550.0
29	9620.0
30	10732.0
31	12726.0
32	15730.0
33	20585.0
34	37227.0
35	73376.0
36	68508.0
37	52687.0
38	107293.0
39	177020.0
40	162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.281265676494307	19.257719978918093	24.64662403718544	29.81439030740216
2	33.85868771470844	19.34820708529918	26.620036702840345	20.173068497152038
3	21.321937249572965	21.829617541163696	38.535918618754	18.312526590509332
4	22.38936760625091	24.251338256678583	35.961163568938474	17.39813056813203
5	20.056387754713267	28.388345260698117	32.25055721007614	19.304709774512478
6	70.74996348765883	1.6578190384871825	23.132949371670232	4.4592681021837555
7	70.94554263688953	1.5787618823858116	19.147547958166385	8.328147522558277
8	58.242740394079284	2.9521021583556113	28.519630939605413	10.28552650795969
9	35.50809303979528	16.088766263866752	30.5317784367638	17.871362259574173
10	28.141013836589813	17.624348334084747	43.120122427467436	11.114515401858002
11	24.059092842946132	14.753208323543793	40.692686736812696	20.49501209669738
12	16.658517535448723	21.11016567077933	44.27740489328871	17.953911900483234
13	11.960649221175888	24.464697328566622	43.63240644903195	19.942247001225542
14	10.305052673020873	21.910579688978352	47.71099370717737	20.0733739308234
15	10.577307738711337	17.530844990824292	51.61448047700993	20.277366793454448
16	10.935922428737435	24.431042474965235	40.52044373606975	24.112591360227583
17	13.287634698789061	21.970745677256303	43.18489214571917	21.55672747823547
18	15.626647024085447	28.69266768689556	40.159765305020926	15.520919983998068
19	15.769521402581898	22.97197757189756	36.61870320864104	24.639797816879497
20	12.207980645284193	31.210272985312514	35.70287844247878	20.878867926924517
21	13.00712466900769	24.32261669661737	36.72919272801163	25.94106590636331
22	11.47884506702396	27.465376775611027	32.382477886221196	28.67330027114382
23	11.154202729218127	23.091357052596823	44.205015208183845	21.549425010001205
24	16.027806529041598	21.317016020980308	40.356138200798824	22.299039249179266
25	12.160990849689805	26.253167048723334	38.783885040100074	22.80195706148678
26	15.825242410195518	23.227563960096774	36.948743022967854	23.99845060673986
27	22.23982575675796	23.3115423447908	38.12856789072968	16.320064007721566
28	17.136987954102402	23.190892869616018	33.89599380242696	25.776125373854626
29	24.756319810008826	22.05170782507096	30.866421981064384	22.32555038385583
30	24.390243902439025	24.970472628444064	27.747950546415122	22.89133292270179
31	30.88166191477067	19.492192708961717	27.908604847568913	21.717540528698702
32	28.516455953416603	23.306779865507586	28.293571922962137	19.883192258113677
33	22.93610022796401	23.48426159346207	29.588807538687206	23.990830639886717
34	30.49447234904528	20.883312907588852	29.7820054482763	18.840209295089565
35	28.84062204329411	18.477784621636893	32.70188149681549	19.979711838253504
36	26.49525974562011	24.664403959842772	27.887808688032205	20.952527606504912
37	24.129260037718836	20.946971380674494	36.09737047643843	18.826398105168245
38	20.773299636146582	23.57982867774525	33.77359808484833	21.873273601259836
39	24.94126275550701	21.92248588718639	35.20535810669224	17.93089325061436
40	17.220331341558666	20.373727624284836	39.31394898432192	23.091992049834584
41	22.379683898375042	23.07881585715102	31.212019227716358	23.329481016757576
42	22.607330408112723	18.69955740692528	36.8384122529067	21.854699932055297
43	25.932969691581842	21.422901810377123	33.32608378153555	19.318044716505483
44	18.98213117772938	23.93796711984303	33.861227703659495	23.218673998768104
45	21.803741403724892	20.271969316933472	34.464316330223966	23.45997294911767
46	26.43953873800649	18.165683479276865	33.33814872905303	22.056629053663617
47	18.69003244835885	16.900133984417167	38.9858141617084	25.424019405515587
48	20.466278471688646	15.408525472914192	32.94587918542554	31.17931686997162
49	22.14870365313911	15.622837040658874	38.792775001428744	23.435684304773275
50	19.614905925159228	15.347724487398478	39.41586604098272	25.62150354645957
51	17.33240835402366	14.212031927661114	38.594973361865875	29.860586356449346
52	16.380547494618398	14.089001212844723	47.185692242238744	22.344759050298133
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	169.0
1	981.5
2	1794.0
3	24664.0
4	47534.0
5	25817.0
6	4100.0
7	3953.5
8	3807.0
9	4102.0
10	4397.0
11	4605.0
12	4813.0
13	4657.5
14	4287.5
15	4073.0
16	3710.5
17	3348.0
18	3054.5
19	2761.0
20	2586.5
21	2412.0
22	2279.0
23	2146.0
24	2150.5
25	2155.0
26	2250.0
27	2345.0
28	2336.5
29	2328.0
30	2541.0
31	2754.0
32	3105.0
33	3456.0
34	3903.0
35	4350.0
36	5921.0
37	7492.0
38	8064.5
39	9672.0
40	10707.0
41	14234.5
42	17762.0
43	23527.0
44	29292.0
45	34953.0
46	40614.0
47	51320.5
48	62027.0
49	77360.5
50	92694.0
51	96832.5
52	100971.0
53	86895.5
54	72820.0
55	55674.0
56	38528.0
57	28783.0
58	19038.0
59	14533.5
60	10029.0
61	7807.5
62	5586.0
63	4541.0
64	2818.5
65	2141.0
66	1734.0
67	1327.0
68	1100.5
69	874.0
70	840.0
71	806.0
72	763.0
73	720.0
74	577.5
75	435.0
76	353.5
77	272.0
78	220.5
79	169.0
80	141.5
81	114.0
82	101.0
83	88.0
84	55.0
85	22.0
86	17.0
87	12.0
88	9.5
89	3.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	629924.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.33842755989714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.06696535404355	22.167368354151645
2	11.764100658838512	7.138086312910209
3	4.641450631433987	4.224429412637966
4	2.3771114823636466	2.8847129803795677
5	1.491509554772372	2.262502729117802
6	0.9341146295377865	1.7003741412523372
7	0.6811783893918008	1.4466116855351387
8	0.5120596576186609	1.2428067863207584
9	0.37646464797309137	1.027921090625443
>10	3.485294768797261	23.315409005386893
>50	0.46051899317180733	9.406307785788085
>100	0.19398574027441118	10.357991298984112
>500	0.009988425650985943	2.0957133171447895
>1k	0.004205652905678291	2.8314458538258336
>5k	5.257066132097864E-4	0.8756062489440559
>10k+	5.257066132097864E-4	7.022712996995387
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44032	6.99004959328427	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	5490	0.8715337088283666	TruSeq Adapter, Index 14 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCTT	4883	0.7751728779979806	Illumina PCR Primer Index 8 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	3395	0.5389539055505109	TruSeq Adapter, Index 14 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	2606	0.4137007004019533	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCG	1644	0.2609838647201885	No Hit
TCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	1593	0.25288764993872276	TruSeq Adapter, Index 14 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	1343	0.2132003225785968	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	1195	0.1897054247814022	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	1094	0.17367174452791131	No Hit
AGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	945	0.15001809742127623	No Hit
CGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	856	0.13588940888107137	No Hit
TCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	854	0.13557191026219037	No Hit
ACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	805	0.12779319409960566	Illumina PCR Primer Index 8 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	774	0.12287196550695005	No Hit
CGTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	765	0.1214432217219855	No Hit
TGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCTTG	724	0.11493450003492484	TruSeq Adapter, Index 14 (96% over 25bp)
GGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	719	0.11414075348772233	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	718	0.11398200417828183	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	710	0.1127120097027578	No Hit
TGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	701	0.11128326591779325	No Hit
ATCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	633	0.10048831287583898	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC	632	0.1003295635663985	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7972390320102107	0.0
2	0.0	0.0	0.0	2.633174795689639	0.0
3	0.0	0.0	0.0	4.358621039998476	0.0
4	0.0	0.0	0.0	5.502251065207866	0.0
5	0.0	0.0	0.0	11.14832900476883	0.0
6	0.0	0.0	0.0	12.720105917539259	0.0
7	0.0	0.0	0.0	17.85850356550949	0.0
8	0.0	0.0	0.0	24.074650275271303	0.0
9	1.5874930944050392E-4	0.0	0.0	28.61265803493755	0.0
10	1.5874930944050392E-4	0.0	0.0	31.28139267594186	0.0
11	1.5874930944050392E-4	0.0	0.0	33.652472361745225	0.0
12	1.5874930944050392E-4	0.0	0.0	35.327118827033104	0.0
13	4.762479283215118E-4	0.0	0.0	36.00386713317797	0.0
14	4.762479283215118E-4	0.0	0.0	36.47741632323899	0.0
15	4.762479283215118E-4	0.0	0.0	37.99236098322972	0.0
16	4.762479283215118E-4	0.0	0.0	39.678437398797314	0.0
17	4.762479283215118E-4	0.0	0.0	41.7089998158508	0.0
18	4.762479283215118E-4	0.0	0.0	42.69737301642738	0.0
19	4.762479283215118E-4	0.0	0.0	43.807348188035384	0.0
20	4.762479283215118E-4	0.0	0.0	44.938913265727294	0.0
21	4.762479283215118E-4	0.0	0.0	45.99634241591049	0.0
22	0.0015874930944050393	0.0	0.0	47.066947758777246	0.0
23	0.0019049917132860472	0.0	0.0	47.851486846032216	0.0
24	0.004921228592655622	0.0	0.0	48.49727903683619	0.0
25	0.004921228592655622	0.0	0.0	49.091318952762556	0.0
26	0.004921228592655622	0.0	0.0	49.70694877477283	0.0
27	0.004921228592655622	0.0	0.0	50.29574996348766	0.0
28	0.004921228592655622	0.0	0.0	50.91217353204514	0.0
29	0.004921228592655622	0.0	0.0	51.617972961817614	0.0
30	0.004921228592655622	0.0	0.0	52.24884271753418	0.0
31	0.004921228592655622	0.0	0.0	52.769381703189595	0.0
32	0.004921228592655622	0.0	0.0	53.31087559769115	0.0
33	0.004921228592655622	0.0	0.0	53.844590776030124	0.0
34	0.004921228592655622	0.0	0.0	54.473714289342844	0.0
35	0.004921228592655622	0.0	0.0	54.96155091725351	0.0
36	0.004921228592655622	0.0	0.0	55.428432636318036	0.0
37	0.004921228592655622	0.0	0.0	55.896108101929755	0.0
38	0.004921228592655622	0.0	0.0	56.362037325137635	0.0
39	0.004921228592655622	0.0	0.0	56.835586515198656	0.0
40	0.004921228592655622	0.0	0.0	57.30437322597647	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATGG	55	1.8189894E-12	46.000004	1
TTCACGG	35	1.0185795E-7	46.000004	1
TCGATGG	35	1.0185795E-7	46.000004	1
TGGCCGC	55	1.8189894E-12	46.000004	36
GTCACGG	55	1.8189894E-12	46.000004	1
TGGATAG	55	1.8189894E-12	46.000004	41
GTGACGG	35	1.0185795E-7	46.000004	1
CGTTTTC	55	1.8189894E-12	46.000004	1
CTAATGG	35	1.0185795E-7	46.000004	1
CCTTCCG	20	6.309271E-4	46.0	14
GTCATAA	30	1.8600531E-6	46.0	44
AACGTGG	30	1.8600531E-6	46.0	1
CGGGTAG	20	6.309271E-4	46.0	5
CTGTCCG	20	6.309271E-4	46.0	36
TGCCCCG	20	6.309271E-4	46.0	42
CAAAAGT	20	6.309271E-4	46.0	36
CGACGGT	30	1.8600531E-6	46.0	27
CATGCGG	80	0.0	46.0	1
TTAGCGC	20	6.309271E-4	46.0	1
TCCGAGG	50	1.6370905E-11	46.0	1
>>END_MODULE