SRR1043373_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1043373_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	612803
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40283	6.573564424456146	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	5260	0.8583508892743671	TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	4809	0.784754643825177	Illumina PCR Primer Index 8 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	3278	0.5349190522892349	TruSeq Adapter, Index 14 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	2525	0.41204106376763816	No Hit
TCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	1602	0.2614216966953491	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC	1471	0.24004451675334487	No Hit
GCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	1372	0.22388924336205926	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	1159	0.1891309278838387	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	1134	0.1850513133910898	No Hit
AGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	966	0.15763630399981723	No Hit
CGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	907	0.14800841379692986	No Hit
TCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	819	0.13364817078245372	No Hit
TGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCTT	807	0.13168995582593426	TruSeq Adapter, Index 14 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	804	0.13120040208680442	No Hit
TGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	786	0.12826307965202519	No Hit
GGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	731	0.11928792776797763	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTC	705	0.11504512869551879	No Hit
CGTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	702	0.11455557495638892	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	700	0.114229205796969	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	676	0.11031277588393008	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC	631	0.10296946979698207	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	35	1.210683E-7	45.000004	24
ATAGGTG	35	1.210683E-7	45.000004	23
CGACGGT	35	1.210683E-7	45.000004	27
GCGGTAC	25	3.888666E-5	45.000004	4
CGGCCAT	25	3.888666E-5	45.000004	7
ATCACGG	25	3.888666E-5	45.000004	1
ACGATGG	35	1.210683E-7	45.000004	1
GCCGATG	25	3.888666E-5	45.000004	8
CTAACGG	35	1.210683E-7	45.000004	1
CCATAGG	50	2.1827873E-11	45.000004	1
TTGGTGG	25	3.888666E-5	45.000004	1
GTCGTGG	25	3.888666E-5	45.000004	1
GACGGTC	35	1.210683E-7	45.000004	28
GCGATAC	25	3.888666E-5	45.000004	8
GCCTAAC	25	3.888666E-5	45.000004	36
CCAACGG	35	1.210683E-7	45.000004	1
TAAGCGG	50	2.1827873E-11	45.000004	1
AGTACGG	75	0.0	45.000004	1
TCAACGG	70	0.0	45.000004	1
CTAATGG	75	0.0	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content