##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1043373_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	612803
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.147037465547655	33.0	31.0	34.0	30.0	34.0
2	32.44482321398557	34.0	31.0	34.0	31.0	34.0
3	32.496009321103195	34.0	31.0	34.0	31.0	34.0
4	36.077693810245705	37.0	35.0	37.0	35.0	37.0
5	36.02164643449853	37.0	35.0	37.0	35.0	37.0
6	36.268858017992734	37.0	37.0	37.0	35.0	37.0
7	36.19413579894354	37.0	37.0	37.0	35.0	37.0
8	36.107496210038136	37.0	37.0	37.0	35.0	37.0
9	37.69278218285485	39.0	38.0	39.0	35.0	39.0
10	37.49229687191479	39.0	37.0	39.0	35.0	39.0
11	37.14827603650765	39.0	37.0	39.0	34.0	39.0
12	37.10880495036741	39.0	37.0	39.0	33.0	39.0
13	37.13669319503984	39.0	37.0	39.0	33.0	39.0
14	38.5101247872481	40.0	37.0	41.0	34.0	41.0
15	38.64012578267404	40.0	38.0	41.0	35.0	41.0
16	38.68856222962355	40.0	38.0	41.0	35.0	41.0
17	38.66170041595749	40.0	37.0	41.0	35.0	41.0
18	38.41873489522734	40.0	37.0	41.0	35.0	41.0
19	38.14775547769838	40.0	37.0	41.0	34.0	41.0
20	37.85729182135205	40.0	35.0	41.0	34.0	41.0
21	37.79904961300777	40.0	35.0	41.0	34.0	41.0
22	37.76931738258462	39.0	35.0	41.0	34.0	41.0
23	37.74135896854291	39.0	35.0	41.0	34.0	41.0
24	37.6470709183865	39.0	35.0	41.0	34.0	41.0
25	37.611429774332045	39.0	35.0	41.0	34.0	41.0
26	37.60604957873901	39.0	35.0	41.0	34.0	41.0
27	37.60936875309031	39.0	35.0	41.0	34.0	41.0
28	37.52977873802837	39.0	35.0	41.0	34.0	41.0
29	37.508770355236514	39.0	35.0	41.0	34.0	41.0
30	37.29849070582226	39.0	35.0	41.0	33.0	41.0
31	37.17872138354414	39.0	35.0	41.0	33.0	41.0
32	36.87577900238739	39.0	35.0	41.0	32.0	41.0
33	36.60055352209437	39.0	35.0	41.0	31.0	41.0
34	36.36706739359958	39.0	35.0	41.0	31.0	41.0
35	36.093933939618445	39.0	35.0	41.0	30.0	41.0
36	36.07544349489151	39.0	35.0	41.0	30.0	41.0
37	35.949553119028465	39.0	35.0	41.0	30.0	41.0
38	35.810733628915	38.0	35.0	40.0	29.0	41.0
39	35.73909559842233	38.0	35.0	40.0	29.0	41.0
40	35.695965914004994	38.0	35.0	40.0	29.0	41.0
41	35.65203172960968	38.0	35.0	40.0	29.0	41.0
42	35.49815356648058	38.0	35.0	40.0	28.0	41.0
43	35.34130544400077	38.0	35.0	40.0	28.0	41.0
44	35.14927635798128	37.0	35.0	40.0	28.0	41.0
45	34.95750836728932	37.0	35.0	40.0	27.0	41.0
46	34.698198279055426	37.0	35.0	39.0	27.0	41.0
47	34.3897794234036	36.0	35.0	39.0	26.0	40.0
48	34.209282591632224	36.0	34.0	39.0	26.0	40.0
49	33.906875455896916	35.0	34.0	37.0	26.0	40.0
50	33.639278854705346	35.0	34.0	37.0	25.0	40.0
51	33.1860581622479	35.0	33.0	37.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	6.0
16	18.0
17	42.0
18	84.0
19	178.0
20	396.0
21	630.0
22	1028.0
23	1679.0
24	2925.0
25	5573.0
26	8463.0
27	10146.0
28	10054.0
29	9736.0
30	10717.0
31	12567.0
32	15129.0
33	20116.0
34	36714.0
35	71225.0
36	63513.0
37	51559.0
38	106100.0
39	174190.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.604797953012632	19.336883141890624	24.85007416739148	29.208244737705265
2	33.46018214662787	19.53466285249909	26.58146255811411	20.423692442758927
3	21.579365636264836	22.01180477249622	37.96946163775308	18.439367953485867
4	22.549497962640523	24.399358358232583	35.45625592564005	17.594887753486844
5	20.34569021365757	28.48011514303944	31.66710998477488	19.507084658528107
6	71.32438973046803	1.588601883476419	22.56467412855355	4.522334257502003
7	71.40794023527953	1.5375251100272027	18.5431533461814	8.511381308511872
8	58.59860346636684	2.8798814627212987	28.04441231521386	10.477102755697999
9	35.77103897990055	16.03973870885097	30.05925232089269	18.12996999035579
10	28.45775885561918	17.645474973196933	42.74881160829827	11.147954562885626
11	24.36100998200074	14.566834692388909	40.453620494677736	20.618534830932617
12	16.791856436734154	21.173036032787046	43.81408054464485	18.221026985833948
13	11.924876346884725	24.457778437768745	43.29955956481936	20.317785650527167
14	10.329910264799617	21.835891795568884	47.57483236864049	20.259365570991005
15	10.57729808763991	17.532224874878224	51.34080609918685	20.549670938295016
16	10.955559943407588	24.450271947102088	40.238380034040304	24.355788075450022
17	13.516905106535052	21.890395445192013	42.823223776646	21.769475671626935
18	15.753023402300576	28.816928115560792	39.76220743044665	15.66784105169198
19	15.88699794224245	22.952890243683534	36.20510996192904	24.955001852144978
20	12.262342057724913	31.21818920599279	35.26924639729244	21.250222338989854
21	13.028004105724026	24.308464547334136	36.243295153581165	26.420236193360676
22	11.599159925783653	27.473592655388433	31.91808786836879	29.009159550459117
23	11.213881133088448	23.013105353596504	43.85340802835495	21.91960548496009
24	16.085593575749467	21.209099824902946	40.02656644957678	22.678740149770807
25	12.268869440913312	26.101047155447997	38.67311354546241	22.956969858176283
26	15.983766398010454	23.11656437713262	36.49672080587073	24.4029484189862
27	22.40964877782909	23.252986685770143	37.760259006564915	16.577105529835855
28	17.32236950537122	23.22720352217597	33.38968640819317	26.06074056425964
29	25.087670915449173	21.85449483765582	30.347109919501047	22.710724327393958
30	24.57641362721788	24.81172579115964	27.301922477533562	23.309938104088914
31	31.2953755121956	19.31632188484717	27.366543571098706	22.021759031858526
32	28.90178409700997	23.22034976982815	27.72326506234467	20.154601070817215
33	23.278769849364313	23.468716700146704	29.141502244603895	24.111011205885088
34	30.982061119152483	20.702085335744115	29.30844006964718	19.00741347545622
35	29.257200111618253	18.20046572879049	32.32099059567267	20.221343563918584
36	26.811063261766016	24.377654809131155	27.527280382113013	21.284001546989817
37	24.52207316217447	20.823168293888898	35.59871606372684	19.05604248020979
38	21.005608654004632	23.395610008436645	33.48743397143944	22.11134736611929
39	25.2585251704055	21.762621919279116	34.90240746210446	18.076445448210926
40	17.291038066066907	20.249411311628695	39.1647886841285	23.29476193817589
41	22.766043899915633	22.77714045133591	30.871421974109136	23.58539367463932
42	22.945546937596582	18.43479878525399	36.55775183868225	22.061902438467172
43	26.28919897585358	21.238962602989865	33.02692708749794	19.444911333658617
44	19.3824116396297	23.682488499566745	33.62826226372913	23.306837597074427
45	21.983084286467268	20.026501175744897	34.29128121109068	23.69913332669716
46	26.886128168432595	17.87246472357348	32.90535457561402	22.3360525323799
47	19.120337204615513	16.537778046125755	38.68600512725949	25.65587962199924
48	20.706164950236865	15.123783662938987	32.62076066859986	31.54929071822429
49	22.40605871707547	15.328253941315563	38.603760099085676	23.66192724252329
50	19.90084904936823	15.051656078707188	39.31426575914283	25.733229112781757
51	17.632746575979557	13.968436838592503	38.25405554476724	30.1447610406607
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	154.0
1	857.5
2	1561.0
3	22575.0
4	43589.0
5	23934.0
6	4279.0
7	3998.5
8	3718.0
9	3986.0
10	4254.0
11	4352.0
12	4450.0
13	4248.5
14	4047.0
15	3816.0
16	3585.0
17	3237.5
18	2890.0
19	2583.5
20	2277.0
21	2177.0
22	2077.0
23	1986.5
24	1896.0
25	1826.0
26	1832.5
27	1909.0
28	2181.0
29	2453.0
30	2629.0
31	2805.0
32	3004.0
33	3203.0
34	4295.0
35	5387.0
36	6701.5
37	8016.0
38	8534.0
39	9052.0
40	12102.0
41	15152.0
42	18697.0
43	22242.0
44	27271.5
45	32301.0
46	36265.0
47	40229.0
48	54470.0
49	68711.0
50	85746.5
51	102782.0
52	101876.0
53	100970.0
54	79046.0
55	57122.0
56	42242.0
57	27362.0
58	20308.0
59	13254.0
60	10072.0
61	6890.0
62	5711.5
63	4533.0
64	3482.0
65	2431.0
66	2000.5
67	1570.0
68	1317.5
69	1065.0
70	903.5
71	742.0
72	751.0
73	760.0
74	639.5
75	427.5
76	336.0
77	270.0
78	204.0
79	172.5
80	141.0
81	108.5
82	76.0
83	52.5
84	29.0
85	21.5
86	14.0
87	10.5
88	7.0
89	4.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	612803.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.85407961013513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.32288865636039	21.59133275581922
2	11.867762954234925	7.086022800598839
3	4.915125615380598	4.402096542461603
4	2.512540666908058	3.000383563743007
5	1.5210477656282773	2.270474054294238
6	0.9562619457062019	1.7128992153153406
7	0.6532108957667242	1.3650707059099223
8	0.5354679625630101	1.2788722546426368
9	0.4035028903729721	1.0841586670902903
>10	3.622840059666176	23.854833995780076
>50	0.47779507861532644	9.59159833597686
>100	0.19608930972073946	10.330694764101073
>500	0.009942556549220593	2.06030137728349
>1k	0.004418914021875818	2.8610716260499887
>5k	5.523642527344773E-4	0.8673911673212069
>10k+	5.523642527344773E-4	6.642798173612201
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40283	6.573564424456146	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	5260	0.8583508892743671	TruSeq Adapter, Index 14 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCT	4809	0.784754643825177	Illumina PCR Primer Index 8 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	3278	0.5349190522892349	TruSeq Adapter, Index 14 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	2525	0.41204106376763816	No Hit
TCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	1602	0.2614216966953491	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCC	1471	0.24004451675334487	No Hit
GCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	1372	0.22388924336205926	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTC	1159	0.1891309278838387	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	1134	0.1850513133910898	No Hit
AGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	966	0.15763630399981723	No Hit
CGGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	907	0.14800841379692986	No Hit
TCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	819	0.13364817078245372	No Hit
TGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGCTT	807	0.13168995582593426	TruSeq Adapter, Index 14 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC	804	0.13120040208680442	No Hit
TGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	786	0.12826307965202519	No Hit
GGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG	731	0.11928792776797763	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTC	705	0.11504512869551879	No Hit
CGTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT	702	0.11455557495638892	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCT	700	0.114229205796969	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT	676	0.11031277588393008	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGC	631	0.10296946979698207	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8069477466657311	0.0
2	0.0	0.0	0.0	2.6406528688665034	0.0
3	0.0	0.0	0.0	4.424913063415159	0.0
4	0.0	0.0	0.0	5.5902141471239535	0.0
5	0.0	0.0	0.0	11.28143954908837	0.0
6	0.0	0.0	0.0	12.918670437318355	0.0
7	0.0	0.0	0.0	18.17549848809487	0.0
8	0.0	0.0	0.0	24.51489304066723	0.0
9	1.6318457970995574E-4	0.0	0.0	29.197148186284988	0.0
10	1.6318457970995574E-4	0.0	0.0	31.890672858977517	0.0
11	1.6318457970995574E-4	0.0	0.0	34.22812877874292	0.0
12	1.6318457970995574E-4	0.0	0.0	35.94205641943659	0.0
13	1.6318457970995574E-4	0.0	0.0	36.65745761688503	0.0
14	1.6318457970995574E-4	0.0	0.0	37.12938742140623	0.0
15	1.6318457970995574E-4	0.0	0.0	38.68861608053486	0.0
16	1.6318457970995574E-4	0.0	0.0	40.399606398793736	0.0
17	1.6318457970995574E-4	0.0	0.0	42.505829769110136	0.0
18	1.6318457970995574E-4	0.0	0.0	43.523775177340845	0.0
19	1.6318457970995574E-4	0.0	0.0	44.6541547609917	0.0
20	1.6318457970995574E-4	0.0	0.0	45.824188197512086	0.0
21	1.6318457970995574E-4	0.0	0.0	46.87036453803261	0.0
22	0.0021213995362294245	0.0	0.0	47.96745446742265	0.0
23	0.00228458411593938	0.0	0.0	48.778155459421704	0.0
24	0.004895537391298672	0.0	0.0	49.47250584608757	0.0
25	0.004895537391298672	0.0	0.0	50.08036840550715	0.0
26	0.004895537391298672	0.0	0.0	50.71140317524555	0.0
27	0.004895537391298672	0.0	0.0	51.296583078085455	0.0
28	0.004895537391298672	0.0	0.0	51.894654562722444	0.0
29	0.004895537391298672	0.0	0.0	52.590636795185404	0.0
30	0.004895537391298672	0.0	0.0	53.2379900228948	0.0
31	0.004895537391298672	0.0	0.0	53.78335288828547	0.0
32	0.004895537391298672	0.0	0.0	54.314518695241375	0.0
33	0.004895537391298672	0.0	0.0	54.84650042509583	0.0
34	0.004895537391298672	0.0	0.0	55.46986551958786	0.0
35	0.004895537391298672	0.0	0.0	55.97916459286263	0.0
36	0.004895537391298672	0.0	0.0	56.46561782497801	0.0
37	0.004895537391298672	0.0	0.0	56.94423819726731	0.0
38	0.004895537391298672	0.0	0.0	57.423184938716034	0.0
39	0.004895537391298672	0.0	0.0	57.90588492549808	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	35	1.210683E-7	45.000004	24
ATAGGTG	35	1.210683E-7	45.000004	23
CGACGGT	35	1.210683E-7	45.000004	27
GCGGTAC	25	3.888666E-5	45.000004	4
CGGCCAT	25	3.888666E-5	45.000004	7
ATCACGG	25	3.888666E-5	45.000004	1
ACGATGG	35	1.210683E-7	45.000004	1
GCCGATG	25	3.888666E-5	45.000004	8
CTAACGG	35	1.210683E-7	45.000004	1
CCATAGG	50	2.1827873E-11	45.000004	1
TTGGTGG	25	3.888666E-5	45.000004	1
GTCGTGG	25	3.888666E-5	45.000004	1
GACGGTC	35	1.210683E-7	45.000004	28
GCGATAC	25	3.888666E-5	45.000004	8
GCCTAAC	25	3.888666E-5	45.000004	36
CCAACGG	35	1.210683E-7	45.000004	1
TAAGCGG	50	2.1827873E-11	45.000004	1
AGTACGG	75	0.0	45.000004	1
TCAACGG	70	0.0	45.000004	1
CTAATGG	75	0.0	45.000004	1
>>END_MODULE